scholarly journals Histomorphometric Evaluation of Peri-Implant Bone Response to Intravenous Administration of Zoledronate (Zometa®) in an Osteoporotic Rat Model

Materials ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5248
Author(s):  
Amani M. Basudan ◽  
Marwa Y. Shaheen ◽  
Abdurahman A. Niazy ◽  
Jeroen J. J. P. van den Beucken ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Femoral Condyle ◽  
Titanium Implants ◽  
Histological Evaluation ◽  
Bone Area ◽  
Treatment Regimens ◽  
Implant Placement ◽  
Bone Response ◽  
Female Wistar Rats ◽  
The Right ◽  
Post Implantation

We evaluated the response to peri-implant bone placed in the femoral condyle of osteoporotic rats, following intravenous zoledronate (ZOL) treatment in three settings: pre-implantation (ZOL-Pre), post-implantation (ZOL-Post), and pre- + post-implantation (ZOL-Pre+Post). Twenty-four female Wistar rats were ovariectomized (OVX). After 12 weeks, the rats received titanium implants in the right femoral condyle. ZOL (0.04 mg/kg, weekly) was administered to six rats 4 weeks pre-implantation and was stopped at implant placement. To another six rats, ZOL was given post-implantation and continued for 6 weeks. Additional six rats received ZOL treatment pre- and post-implantation. Control animals received weekly saline intravenous injections. At 6 weeks post-implantation, samples were retrieved for histological evaluation of the percentage of bone area (%BA) and of the percentage of bone-to-implant contact (%BIC). BA% for ZOL-Pre (29.6% ± 9.0%) and ZOL-Post (27.9% ± 5.6%) rats were significantly increased compared to that of the controls (17.3% ± 3.9%, p < 0.05). In contrast, ZOL-Pre+Post rats (20.4% ± 5.0%) showed similar BA% compared to Saline controls (p = 0.731). BIC% revealed a significant increase for ZOL-Post (65.8% ± 16.9%) and ZOL-Pre+Post (68.3% ± 10.0%) rats compared with that of Saline controls (43.3% ± 9.6%, p < 0.05), while ZOL-Pre rats (55.6% ± 19%) showed a BIC% comparable to that of Saline controls (p = 0.408). Our results suggest that receiving intravenous ZOL treatment before or after implant placement enhances peri-implant bone responses in terms of bone area. However, the effect of different ZOL treatment regimens on BIC% was found to be inconclusive.

scholarly journals Effect of Osteoporosis on Well-Integrated Bone Implants

2021 ◽  
Vol 11 (2) ◽  
pp. 723
Author(s):  
Amani M. Basudan ◽  
Marwa Y. Shaheen ◽  
Abdurahman A. Niazy ◽  
Jeroen J.J.P. van den Beucken ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Dental Implants ◽  
Femoral Condyle ◽  
Bone Implants ◽  
Bone Implant ◽  
Ovx Rats ◽  
Bone Response ◽  
Significant Difference ◽  
Female Wistar Rats ◽  
Edentulous Patients

The installation of dental implants has become a common treatment for edentulous patients. However, concern exists about the influence of osteoporosis on the final implant success. This study evaluated whether an ovariectomy (OVX)-induced osteoporotic condition, induced eight weeks postimplantation in a rat femoral condyle, influences the bone response to already-integrated implants. The implants were inserted in the femoral condyle of 16 female Wistar rats. Eight weeks postimplantation, rats were randomly ovariectomized (OVX) or sham-operated (SHAM). Fourteen weeks later, animals were sacrificed, and implants were used for histological and histomorphometric analyses. A significant reduction in the quantity and quality of trabecular bone around dental implants existed in OVX rats in comparison to the SHAM group. For histomorphometric analysis, the bone area (BA%) showed a significant difference between OVX (34.2 ± 4.3) and SHAM (52.6 ± 12.7) groups (p < 0.05). Bone–implant contact (BIC%) revealed significantly lower values for all implants in OVX (42.5 ± 20.4) versus SHAM (59.0 ± 19.0) rats. Therefore, induction of an osteoporotic condition eight weeks postimplantation in a rat model negatively affects the amount of bone present in close vicinity to bone implants.

scholarly journals Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

2017 ◽  
Vol 28 (5) ◽  
pp. 559-565 ◽  
Author(s):  
Suelen Cristina Sartoretto ◽  
José de Albuquerque Calasans-Maia ◽  
Yanan Osório da Costa ◽  
Rafael Seabra Louro ◽  
José Mauro Granjeiro ◽  
...  
Keyword(s):  
Bone Healing ◽  
Experimental Period ◽  
Bone Area ◽  
Histomorphometric Analysis ◽  
Implant Placement ◽  
Healing Period ◽  
Post Test ◽  
Bone To Implant Contact ◽  
Etched Surface ◽  
Post Implantation

Abstract The objective of this study was to evaluate the early osseointegration of two different implants surfaces, a sandblasted and acid-etched surface (TN) compared with same geometry and surface roughness modified to be hydrophilic/wettable by conditioning in an isotonic solution of 0.9% sodium-chloride (TA) through histological and histomorphometric analysis after sheep tibia implantation. Forty dental implants, divided in two groups (TN and TA) were placed in the left tibia of twenty healthy, skeletally mature Santa Ines sheep (n=5/experimental period). After 7, 14, 21 and 28 days post-implantation, the samples were removed and the sheep were kept alive. Analysis of resin sections (30 μm) allowed the quantification of bone area (BA) and bone-to-implant contact (BIC). TA group presented nearly 50% increase in BA at 14 days (p<0.001, ANOVA - Tukey’s post test) compared with 7 days. The TA presented higher values than the TN for BA and BIC at 14, 21, and 28 days after placement, stabilizing bone healing. TA hydrophilic surface promoted early osseointegration at 14 and 21 days compared to TN, accelerating bone healing period post-implant placement in sheep tibia.

Sequential Bone Response to Immediately Loaded Mini-Implants, in vivo Study

2006 ◽  
Vol 925 ◽  
Author(s):  
Glaucio Serra Guimarães ◽  
Liliane Siqueira de Morais ◽  
Carlos Nelson Elias ◽  
Marc Andre Meyers
Keyword(s):  
Dental Implants ◽  
Titanium Implants ◽  
Test Material ◽  
Removal Torque ◽  
Bone Implant ◽  
Subcutaneous Injections ◽  
Mini Implants ◽  
Bone Response ◽  
The Right

ABSTRACTThe use of osseointegrated titanium implants has been related like an excellent alternative to traditional orthodontic anchorage methodologies, and they are a necessity when dental elements lack quantity or quality, when extraoral devices are impractical, or when noncompliance during treatment is likely. In orthodontics, the implants can be use to anchor different movements. However, conventional dental implants can only be placed in limited areas such as the retromolar or the edentulous areas. Another limitation has been the direction of the force application and conventional dental implants are troublesome for patients because of the severity of the surgery, the discomfort of the initial healing, and the difficulty of oral hygiene. Due to these factors, mini-implants became widely used. They have little limitations related to the local of implantation, the surgical procedure of insertion is relatively simple and the control of direction and quantify of the force is simple to be done. These improvements were obtained due to decrease of the size, but these changes could result on significant changes to the bone-implant interface. Since, the orthodontic treatment has to be done as fast as possible. The purpose of this work is to analyze the bone healing reactions to immediately loaded mini-implant of titanium alloy grade 4 by histological, fluorescent and SEM observation, by histomorphometric analysis and by removal torque test. Material and method: Seventy two mini-implants were inserted in eighteen New Zealand rabbits. Four mini-implants were put in the right tibiae of each rabbit and two of then were loaded immediately with 100 gf. Subcutaneous injections of fluorescent labels were administrated in defined periods. The animals were euthanized after 1, 4 and 12 weeks, performing three time analysis and the tibias were dissected and prepared to microcopy analysis and to removal torque test. Results: The results indicated that all the mini-implants remained stable during experimental time. The SEM findings indicated no differences between load and unload group in one and four weeks period, although, the 12 weeks loaded group demonstrated more mature bone formation than the unload group in the same time. These findings suggest that the force can be applied after insertion of the mini-implant without compromises their stability.

scholarly journals Effect of Systemic Zoledronic Acid Dosing Regimens on Bone Regeneration in Osteoporotic Rats

2021 ◽  
Vol 11 (4) ◽  
pp. 1906
Author(s):  
Marwa Y. Shaheen ◽  
Amani M. Basudan ◽  
Abdurahman A. Niazy ◽  
Jeroen J. J. P. van den Beucken ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Femoral Condyle ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Favorable Effect ◽  
Osteoporotic Bone ◽  
Bovine Bone ◽  
Graft Placement ◽  
Regeneration Of Bone

The aim of this study was to evaluate the regeneration of bone defects created in the femoral condyle of osteoporotic rats, following intravenous (IV) zoledronate (ZA) treatment in three settings: pre-bone grafting (ZA-Pre), post-bone grafting (ZA-Post), and pre- plus post-bone grafting (ZA-Pre+Post). Twenty-four female Wistar rats were ovariectomized (OVX). After 12 weeks, bone defects were created in the left femoral condyle. All defects were grafted with a particulate inorganic cancellous bovine bone substitute. ZA (0.04 mg/kg, weekly) was administered to six rats 4 weeks pre-bone graft placement. To another six rats, ZA was given post-bone graft placement creation and continued for 6 weeks. Additional six rats received ZA treatment pre- and post-bone graft placement. Control animals received weekly saline intravenous injections. At 6 weeks post-bone graft placement, samples were retrieved for histological evaluation of the bone area percentage (BA%) and remaining bone graft percentage (RBG%). BA% for ZA-Pre (50.1 ± 3.5%) and ZA-Post (49.2 ± 8.2%) rats was significantly increased compared to that of the controls (35.4 ± 5.4%, p-value 0.031 and 0.043, respectively). In contrast, ZA-Pre+Post rats (40.7 ± 16.0%) showed similar BA% compared to saline controls (p = 0.663). For RBG%, all experimental groups showed similar results ranging from 36.3 to 47.1%. Our data indicate that pre- or post-surgical systemic IV administration of ZA improves the regeneration of bone defects grafted with inorganic cancellous bovine-bone particles in osteoporotic bone conditions. However, no favorable effect on bone repair was seen for continued pre- plus post-surgical ZA treatment.

scholarly journals A tissue-engineered urinary conduit in a porcine urinary diversion model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arkadiusz Jundziłł ◽  
Piotr Kwieciński ◽  
Daria Balcerczyk ◽  
Tomasz Kloskowski ◽  
Dariusz Grzanka ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Urinary Diversion ◽  
Porcine Model ◽  
Histological Evaluation ◽  
Metabolic Complications ◽  
Group 3 ◽  
Group 2 ◽  
The Right ◽  
Group 1

AbstractThe use of an ileal segment is a standard method for urinary diversion after radical cystectomy. Unfortunately, utilization of this method can lead to numerous surgical and metabolic complications. This study aimed to assess the tissue-engineered artificial conduit for urinary diversion in a porcine model. Tissue-engineered tubular polypropylene mesh scaffolds were used for the right ureter incontinent urostomy model. Eighteen male pigs were divided into three equal groups: Group 1 (control ureterocutaneostomy), Group 2 (the right ureter-artificial conduit-skin anastomoses), and Group 3 (4 weeks before urostomy reconstruction, the artificial conduit was implanted between abdomen muscles). Follow-up was 6 months. Computed tomography, ultrasound examination, and pyelogram were used to confirm the patency of created diversions. Morphological and histological analyses were used to evaluate the tissue-engineered urinary diversion. All animals survived the experimental procedures and follow-up. The longest average patency was observed in the 3rd Group (15.8 weeks) compared to the 2nd Group (10 weeks) and the 1st Group (5.8 weeks). The implant’s remnants created a retroperitoneal post-inflammation tunnel confirmed by computed tomography and histological evaluation, which constitutes urostomy. The simultaneous urinary diversion using a tissue-engineered scaffold connected directly with the skin is inappropriate for clinical application.

Long-Term (9–12 Years) Outcomes of Titanium Implants With an Oxidized Surface: A Retrospective Investigation on 209 Implants

2015 ◽  
Vol 41 (4) ◽  
pp. 437-443 ◽  
Author(s):  
Marco Mozzati ◽  
Giorgia Gallesio ◽  
Massimo Del Fabbro
Keyword(s):  
Survival Rate ◽  
Bone Remodeling ◽  
Titanium Implants ◽  
Pocket Depth ◽  
Long Term Study ◽  
Bone Response ◽  
Single Tooth ◽  
Clinical Records

The aim of this paper is to retrospectively assess the long-term clinical and radiological results in a group of patients treated with Brånemark TiUnite implants supporting mostly single-tooth and partial restorations. The clinical records of 90 consecutive patients (mean age 55.9 years; range 21–82 years), treated with 209 Brånemark System MkIII or MkIV TiUnite implants (72 maxillary/137 mandibular; 26 anterior intercanine/183 posterior sites), were analyzed. Indication types were single tooth (n = 21 implants), partial (n = 180) and full arches (n = 8). A delayed loading protocol was applied in 128 implants, while 81 were immediately loaded. Cumulative survival rate and marginal bone remodeling were evaluated. Marginal bone level was evaluated by an independent radiologist from periapical radiographs taken at implant insertion and at long-term follow up. Plaque, probing pocket depth and peri-implant mucosa conditions were also assessed. The results showed the mean follow-up duration was 11.0 years (range 9.6–12.4 years): 181 implants (90.5%) reached at least 10 years follow-up, 100 implants 11 years, and 17 implants 12 years. Overall, 6 implants failed in 4 patients (5 during the first year and 1 after 2 years) resulting in a 97.1% survival rate after 12 years. Mean bone levels at implant insertion and at the last follow up were −0.90 ± 1.16 mm (mean ± SD; n = 169) and −1.49 ± 0.95 mm (n = 195), respectively. Mean marginal bone remodeling from implant insertion to the last follow-up was −0.60 ± 1.17 mm (n = 168). At the last available follow-up, mean pocket depth was 1.65 ± 0.84 mm. Peri-implant mucosa was normal for the majority (97%) of implants. In conclusion, this retrospective long-term study showed excellent survival rate of TiUnite implants as well as favorable marginal bone response and soft tissue conditions.

scholarly journals Influence of the Thermal Treatment to Address a Better Osseointegration of Ti6Al4V Dental Implants: Histological and Histomorphometrical Study in a Rabbit Model

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio Scarano ◽  
Ezio Crocetta ◽  
Alessandro Quaranta ◽  
Felice Lorusso
Keyword(s):  
Thermal Treatment ◽  
Dental Implants ◽  
Bone Healing ◽  
Rabbit Model ◽  
Titanium Implants ◽  
Control Group ◽  
Left Femur ◽  
Bone Response ◽  
First Choice ◽  
Significant Difference

Background. Pure titanium continues to be the first choice for dental implants and represents the gold standard for their biocompatibility and physical and mechanical characteristics, while the titanium alloy (Ti6Al4V) has good mechanical properties. The surface structure of the titanium oxide layer formation on the surface influences and improves the bone response around dental implants. Purpose. The purpose of this study is to evaluate the influence of a thermal treatment of Ti6Al4V implant surfaces and the bone healing response in a rabbit model. Methods. Altogether sixteen implants with same design were inserted into the distal femoral metaphysis. A screw (13 mm long, 4 mm in diameter) was inserted in an implant bed. Each rabbit received two implants, one in the left femur and one in the right femur. The samples were histologically and histomorphometrically evaluated at 8 weeks. Results. A statistically significant difference (p = 0.000034) was present histologically in the percentages of bone-implant contact (BIC) between the test group (BIC = 69.25±4.49%.) and control group (BIC = 56.25 ± 4.8%) by one-way analysis of variance (ANOVA). Significance was set at p ≤ 0.05. Conclusions. The outcome of the present study indicates a novel approach to improving bone healing around titanium implants.

Bone Response to Submerged, Unloaded Implants Inserted in Poor Bone Sites: A Histological and Histomorphometrical Study of 8 Titanium Implants Retrieved From Man

2005 ◽  
Vol 31 (5) ◽  
pp. 225-233 ◽  
Author(s):  
Giovanna Iezzi ◽  
Marco Degidi ◽  
Antonio Scarano ◽  
Vittoria Perrotti ◽  
Adriano Piattelli
Keyword(s):  
Titanium Implants ◽  
Bone Response
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