scholarly journals Use of DNA Markers for Grape Phylloxera Population and Evolutionary Genetics: From RAPDs to SSRs and Beyond

Insects ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 317 ◽  
Author(s):  
Javier Tello ◽  
Astrid Forneck
Keyword(s):  
Molecular Level ◽  
Evolutionary Genetics ◽  
Reproductive Mode ◽  
Native Range ◽  
Daktulosphaira Vitifoliae ◽  
Grape Phylloxera ◽  
The World ◽  
Major Pest ◽  
Different Populations ◽  
Adequate Management

Grape phylloxera (Daktulosphaira vitifoliae Fitch) is a major pest of cultivated grapevines (Vitis spp.), occurring in virtually all viticultural regions around the world. Different grape phylloxera strains can be found at varying levels on leaves and roots on both own-rooted plants and in plants grafted onto partially resistant rootstocks. Considering its relevance for the adequate management of the pest in infested vineyards, the analysis of its genetic diversity has received considerable attention from the scientific community in the last decades. Here, we review 25 years of DNA-based molecular markers applied to the analysis of the genetic structure and the reproductive mode of grape phylloxera in its native range and in different introduced regions. The use given to RAPD, AFLP, mtDNA sequencing and microsatellite (SSR) genetic markers for the analysis of grape phylloxera diversity is discussed, and an overview of the main findings obtained after their application to different populations collected in diverse regions all around the world is shown. Lastly, we explore how recent advancements in molecular biology and in modern high throughput genotyping technologies may be applied to better understand grape phylloxera natural diversity at a molecular level.

Reproductive mode of grape phylloxera (Daktulosphaira vitifoliae, Homoptera: Phylloxeridae) in Europe: molecular evidence for predominantly asexual populations and a lack of gene flow between them

Genome ◽  
2006 ◽  
Vol 49 (6) ◽  
pp. 678-687 ◽  
Author(s):  
Sonja Vorwerk ◽  
Astrid Forneck
Keyword(s):  
Genetic Structure ◽  
Reproductive Mode ◽  
Genotypic Diversity ◽  
Molecular Evidence ◽  
Main Mode ◽  
Daktulosphaira Vitifoliae ◽  
Grape Phylloxera ◽  
Multiple Introductions ◽  
Important Pest ◽  
Asexual Populations

The genetic structure of European grape phylloxera populations, Daktulosphaira vitifoliae (Homoptera: Phylloxeridae), was analyzed using 6 polymorphic microsatellite markers. Genetic diversity data of 6 populations originating from northern and southern European viticultural regions was assessed for geographic differences, and the structure of 2 additional populations was examined in more detail, focusing on specific host plant and habitat charac ter istics. To test for "signatures" of clonal reproduction, different population genetic measures were applied to the data obtained from these populations. A total of 195 multilocus genotypes were detected in 360 individuals tested. Significant deviations from Hardy–Weinberg equilibrium, negative FISvalues (from –0.148 to –0.658 per population), and the presence of multicopy genotypes revealed that the current major reproductive mode at each of the locations tested was asexual. The high genotypic diversity detected within and among populations, however, together with the occurrence of unique D. vitifoliae genotypes, indicates sexual recombination events took place, probably prior to the multiple introductions into Europe. The absence of overlapping genotypes between the sampling sites suggests low migration rates among the populations studied and implies that the main mode of insect dispersal is through infested plant material carried by human agency. The specific features of European D. vitifoliae habitats are illustrated to discuss the role of habitat and life cycle in the genetic structure of this globally important pest aphid species.Key words: Daktulosphaira vitifoliae, microsatellites, genetic structure, asexual reproduction, parthenogenesis.

scholarly journals Where Did You Come From? Where Did You Go? Investigating the Origin of Invasive Leptocybe Species Using Distribution Modelling

Forests ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 115 ◽  
Author(s):  
Beryn Otieno ◽  
Helen Nahrung ◽  
Martin Steinbauer
Keyword(s):  
New South ◽  
Reproductive Mode ◽  
Invasive Population ◽  
Native Range ◽  
Niche Modelling ◽  
Distribution Modelling ◽  
South Wales ◽  
Eucalypt Plantations ◽  
Different Populations ◽  
Leptocybe Invasa

Research Highlights: We present the first attempts to model the distributions of the two cryptic and globally invasive species of Leptocybe invasa sensu lato (Fisher & LaSalle) (Hymenoptera: Eulophidae) in its purported country of origin, namely Australia. Background and Objectives: Leptocybe invasa is an invasive eucalypt-galling wasp that spread quickly all over the world in the early to mid-2000’s, achieving significant pest status through its severe impacts on the growth and productivity of extra-limital eucalypt plantations. Until its discovery in Europe and the Middle East, the genus was undescribed, and its native range remains unclear. Molecular studies indicate the globally invasive population comprises two cryptic species with variable modes of reproduction. Collection records from Australia, the purported origin, represent only one of the invasive lineages, restricted to subtropical and tropical Queensland and northern New South Wales. To date, the original invasive lineage has not been found in Australia, despite searches over the seventeen years that it has been spreading overseas. Materials and Methods: To understand the distributions of the invasive populations, and to infer Leptocybe spp. native ranges within Australia, we used correlative niche modelling in Maximum Entropy (MaxEnt) and multivariate analysis, and created a CLIMEX model based on development rates of an invasive population. Results: We used the environmental conditions in the extra-limital range to infer possible origins, with our findings supporting the possibility that the invasive populations may have originated from different populations in Australia. Conclusions: We highlight the need for better understanding of the distribution, genetic diversity, and reproductive mode of the species within Australia. The variety of climatic niches occupied by invasive lineages of the wasp potentially present new threats to eucalypts in previously uninfested habitats.

Immunomodulatory Effect of “Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy” to Cure or Prevent Hospital Acquired (Nosocomial) Infections due to Clostridium difficile (C. diff), other Pathogenic Bacteria, and Autoimmune Diseases

2018 ◽  
Vol 11 (1) ◽  
pp. 3937-3949
Author(s):  
Malireddy S Reddy
Keyword(s):  
Immune System ◽  
Gastrointestinal Tract ◽  
Pathogenic Bacteria ◽  
Molecular Level ◽  
Host Immune System ◽  
Hospital Acquired Infections ◽  
The World ◽  
Probiotic Strains ◽  
The Individual ◽  
Hospital Acquired

The worldwide popularity of Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy to treat or prevent the hospital acquired infections (nosocomial infections) arose a great interest in the medical community around the world (Reddy and Reddy, 2016; 2017). The following questions were raised on this subject: Does Multiple Mixed Strain Probiotics directly inhibit the pathogenic bacteria (C. diff) in the gastrointestinal tract or indirectly through modulation of the host immune system or both? To be more specific, what is the exact and/or hypothetical mechanism at molecular level behind the breakthrough discovery of Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy?  To answer these questions, the specific immunomodulation regulatory functions of the individual Probiotic strains (on host) have beenresearched, investigated andoutlined in this article.  A detailed explanation(s) and hypotheses have been proposed outlining the possible cumulativedirect bacteriological and indirect immunomodulatory effects (at the molecular level) of the Multiple Mixed Strain Probiotics used in Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy to successfully treat C. diff infection.  A detailed scientific and research attempts were made to correlate the Probiotic induced immune activities in relation to the reduction of the symptoms associated with the hospital acquired Clostridium difficile infection during and after the Multiple Mixed Strain Probioitc Therapy.  Results of the clinical trials, microbiological tests on feces, and the clinical blood tests significantly revealed that the reasons for the success of Dr. Reddy’s Multiple Mixed Strain Probiotic Therapy are multifold. Presumably, it is predominantly due to the immunomodulatory effect they have exerted on the host immune system along with the direct inhibition of C. diff bacteria by multiple Probiotics, due to the production of bacteriocins, lactic acid and nutritional competency.In addition, the size of the individual cells of the Probiotic strains in the Multiple Mixed Strain Probiotics and their significant effect on immunomodulation has been thoroughly discussed. Results clearly proved that if Probiotics are absent in the GI tract during C. diff infection, the chances of patient survival is zero.  This is because of the excess immune stimulation and incurable damage to the epithelial cell barrier of the gastrointestinal tract caused by C. diff bacteria.  The results also revealed, without any doubt, as of to-datethe latest discovery of Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy is the best way to cure the deadly hospital acquired infections affecting millions of people around the world, with high degree of mortality.  This has been attested by several practicng medical professionals and scientists around the world (Reddy and Reddy, 2017).

scholarly journals Epigenetic polymorphism in wild populations of Mus musculus

1963 ◽  
Vol 4 (2) ◽  
pp. 193-220 ◽  
Author(s):  
R. J. Berry
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Stabilizing Selection ◽  
British Isles ◽  
Wild Populations ◽  
Local Populations ◽  
The World ◽  
Different Populations ◽  
Different Parts

It has been suggested (Berry & Searle, 1963) that the discontinuous (‘quasi-continuous’) variants studied by Grüneberg et al. in the skeleton of rodents can be regarded as constituting epigenetic polymorphism in different populations. Comparisons have been made between the incidences of skeletal variants in house mouse populations collected from: corn ricks on a single farm in Hampshire; eleven separated localities in different parts of the British Isles; and nine other places throughout the world. These showed that the method could profitably be used for genetically characterizing and hence comparing populations. There was evidence suggestive of genetical drift between local populations and stabilizing selection over a larger area.

Quelea quelea (weaver bird).

2021 ◽  
Author(s):  
Robert Cheke
Keyword(s):  
Bird Species ◽  
Breeding Population ◽  
Sub Saharan Africa ◽  
Grain Crops ◽  
The World ◽  
Major Pest ◽  
Sub Saharan ◽  
Economical Losses

Abstract The red-billed quelea is a small weaver bird native to sub-Saharan Africa and renowned for its attacks on small-grain crops within Africa. It is the most numerous bird species in the world, with peak post-breeding population estimated at 1,500,000,000. The red-billed quelea is mainly granivorous, except when feeding its chicks insects or when eating insects prior to migration or breeding, and it relies on a supply of grass seeds to survive. When unable to find grass seeds or when opportunities arise, quelea will attack crops. It is a major pest throughout much of sub-Saharan Africa and can cause significant economical losses.

scholarly journals Genetic diversity of rice stem borer (Chilo suppressalis Walker) from Northern Iran and comparison with other countries

2016 ◽  
Vol 48 (3) ◽  
pp. 360 ◽  
Author(s):  
M. Shayanmehr ◽  
E. Yoosefi-Lafooraki
Keyword(s):  
Genetic Diversity ◽  
Stem Borer ◽  
Chilo Suppressalis ◽  
Mazandaran Province ◽  
Northern Iran ◽  
Low Genetic Diversity ◽  
High Gene ◽  
Major Pest ◽  
Different Populations ◽  
Low Levels

Rice striped stem borer, <em>Chilo suppressalis</em> Walker (Lepidoptera: Crambidae) is considered the major pest of rice in Iran. Because of the serious damage on rice in Northern Iran, the present study was conducted to investigate genetic diversity within populations of <em>C. suppressalis</em>, from Mazandaran using a template of cytochrome oxidase I gene, 750 bps, (<em>COI</em>). Later the haplotypes from Iran were compared with those found in other countries. According to the results of this study, there is very low genetic diversity (two haplotypes) among different populations of this pest in populations of Northern Iran. The genetic similarity and low levels of genetic diversity of these populations suggest that the pest colonization occurred relatively recently and there is high gene flow between these populations of the province. In addition, haplotypes of Mazandaran province are different with those found in other countries. The similarity of Iranian population (Simorgh) with one population from China indicated that China might be the origin of <em>C. suppresalis</em>.

scholarly journals Contributions to the Knowledge of Morphology of the Granary Weevil (Sitophilus Granarius L.), Major Pest of the Stored Cereals

1970 ◽  
Vol 66 (1) ◽  
Author(s):  
Adrian DINUŢĂ ◽  
Horia BUNESCU ◽  
Ilonka BODIŞ
Keyword(s):  
Sexual Dimorphism ◽  
Scientific Literature ◽  
Sitophilus Granarius ◽  
Granary Weevil ◽  
Complex Study ◽  
Sexual Pheromones ◽  
The World ◽  
Major Pest

To solve the multiple practical aspects raised by the achieving of cereals stocks protection (especially wheat and corn) against granary weevil Sitophilus granarius L., the research aimed a complex study on the sexual dimorphism at this species, aspect which help to prevent the weevils damages using unpollutant methods (with the aid of sexual pheromones, ovogenesis inhibitors, etc.). Due to the summarily data in the scientific literature of the world refering to the sexual dimorphism of the species, a detailed study was made within the Entomology Laboratory at the Faculty of Agriculture of USAMV Cluj-Napoca and at the Center of Electronical Microscopy of Babeş-Bolyai University in Cluj-Napoca (Romania), during 2005-2009

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