scholarly journals Influence of Abscisic Acid-Biosynthesis Inhibitor Fluridone on the Feeding Behavior and Fecundity of Nilaparvata lugens

Insects ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 57
Author(s):  
Xu Ding ◽  
Xi Huang ◽  
Litong Sun ◽  
Jincai Wu ◽  
Jinglan Liu
Keyword(s):  
Abscisic Acid ◽  
Feeding Behavior ◽  
Brown Planthopper ◽  
Rice Cultivar ◽  
Nilaparvata Lugens ◽  
Electrical Penetration Graph ◽  
Callose Deposition ◽  
Abscisic Acid Biosynthesis ◽  
Biosynthesis Inhibitor ◽  
Deposition Area

Fluridone (FLU) was a pyrrolidone herbicide that was used for selective weeding in wheat, rice, corn and pasture and was also a biosynthesis inhibitor of abscisic acid (ABA), a significant plant hormone. ABA-promoted callose deposition facilitates rice resistance to pests but whether FLU had the opposite influence was unknown. The effects of FLU on the feeding behavior of the brown planthopper (Nilaparvata lugens Stål; BPH), after feeding with rice plants treated with FLU, were studied, using an electrical penetration graph (EPG). For susceptible rice cultivar (TN1), the duration for which BPH sucked phloem sap (N4 wave duration) after 15 μmol/L of FLU treatment was longer than that of the control but decreased after 30 and 60 μmol/L FLU treatments. Fecundity of BPH treated with 15 μmol/L FLU had no significant change, while the deposition area of callose was significantly decreased. For moderately-resistant rice cultivar (IR42), no differences in BPH feeding behavior and fecundity were observed but the deposition area of callose declined after treated with 15 μmol/L of FLU. These findings suggested that a low concentration of FLU (15 μmol/L) promoted BPH feeding behavior in TN1 but not in IR42 and the response in IR42 appeared to be more complicated, which provided supplementary evidence that ABA promoted plant resistance to BPH.

Candidate genes and molecular markers associated with brown planthopper (Nilaparvata lugens Stål) resistance in rice cultivar Rathu Heenati

2018 ◽  
Vol 38 (7) ◽  
Author(s):  
Lucia Kusumawati ◽  
Pantharika Chumwong ◽  
Watchareewan Jamboonsri ◽  
Samart Wanchana ◽  
Jonaliza L. Siangliw ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Candidate Genes ◽  
Brown Planthopper ◽  
Rice Cultivar ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål ◽  
Rathu Heenati

scholarly journals Phenotypic and transcriptomic responses of two Nilaparvata lugens populations to the Mudgo rice containing Bph1

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pin-Jun Wan ◽  
Ruo-Nan Zhou ◽  
Satyabrata Nanda ◽  
Jia-Chun He ◽  
San-Yue Yuan ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Model Systems ◽  
Plant Interactions ◽  
Electrical Penetration Graph ◽  
Incompatible Interaction ◽  
Rice Varieties ◽  
P450 Gene ◽  
Transcriptomic Responses

Abstract The Bph1 gene was the first reported brown planthopper (BPH, Nilaparvata lugens) resistance gene in Mudgo rice and was widely used as a commercial cultivar for controlling BPH infestations. However, rapid adaptations of BPH on the Mudgo rice resulted in its resistance breakdown and the emergence of virulent BPH populations. Thus, specific BPH populations and rice varieties can serve as good model systems for studying the roles of different bio-compounds and proteins in the insect-plant interactions. Although our understandings have been improved on the complexity of BPH and rice interactions, the underlying molecular mechanisms remain largely unknown. Here we analyzed the feeding performances and the transcriptomic responses of two BPH populations (Mugdo-BPH and TN1-BPH) during compatible (Mudog-BPH feeding on Mudgo rice) and incompatible (TN1-BPH feeding on Mudgo rice) interactions. The electrical penetration graph (EPG) results indicated that the BPH feeding and performances during the incompatible interaction are significantly affected in terms of decreased honeydew, loss of weight, decreased phloem sap ingestion (N4 waveform), but increased non-penetration (NP waveform) phase. Abundance of glucose and trehalose was reduced in BPH during the incompatible interaction. Transcriptomic surveys of insects in both interactions revealed that genes involved in cuticle formation, detoxification, metabolite transport, digestion, RNA processing, lipid or fatty acid metabolism, and proteolysis were significantly down-regulated during the incompatible interaction, whereas genes involved in insulin signaling were significantly upregulated. Knockdown of four genes, including the sugar transporter NlST45, the serine and arginine-rich protein NlSRp54, the cytochrome P450 gene NlCYP6AY1, and the cuticle protein NlCPR70 through RNA-interference revealed thess genes are important for BPH survival. Overall, the results of this study will be helpful for the future researches on BPH virulence shifts.

scholarly journals Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jinghua Zhu ◽  
Xiaoqing Liu ◽  
Kunmiao Zhu ◽  
Hanyu Zhou ◽  
Liang Li ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Target Genes ◽  
Transient Receptor Potential ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Survival Rates ◽  
Nilaparvata Lugens ◽  
Leg Extension ◽  
Significant Difference ◽  
Plant Sap

Abstract The vanilloid-type transient receptor potential (TRPV) channel is reported to be the molecular target of the commercial insecticide pymetrozine, which specifically disrupts the feeding of plant sap-sucking insects. However, the functions of TRPV channels in plant sap-sucking insects have not been fully elucidated. In the present study, RNA interference was used to investigate the effects of the knockdown of TRPV genes (Nan and Iav) on the mortality, locomotion, and feeding behavior of an important plant-feeding insect pest in rice, the brown planthopper, Nilaparvata lugens. Injecting dsRNA of Nan and Iav into fourth-instar nymphs significantly knocked down the target genes. The injection of dsNan or dsIav did not affect any morphological phenotype (including leg extension) of N. lugens nymphs and adults. Knockdown of Nan or Iav resulted in significantly decreased climbing activity against top plants but did not influence the leg-griping strength of adults. Knockdown of Nan resulted in a significantly elevated mortality of N. lugens in the observation period of 7 d after injection, whereas no significant difference in survival rates 7 d after injection was found between dsIav-injected and dsGFP-injected insects. Electropenetrographic (EPG) recordings indicated that knockdown of Nan and Iav reduced the ingestion activity in the rice phloem tissues of N. lugens. Knockdown of Nan and Iav significantly reduced the amount of honeydew excreted by N. lugens. Our findings indicated a relationship between TRPV and N. lugens locomotion and feeding behavior, which may help to fully elucidate the functions of TRPV in insects.

scholarly journals Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens [Stål]) in the rice cultivar ADR52

2011 ◽  
Vol 124 (3) ◽  
pp. 495-504 ◽  
Author(s):  
Khin Khin Marlar Myint ◽  
Daisuke Fujita ◽  
Masaya Matsumura ◽  
Tomohiro Sonoda ◽  
Atsushi Yoshimura ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Rice Cultivar ◽  
Nilaparvata Lugens ◽  
Major Genes ◽  
Nilaparvata Lugens Stål

scholarly journals Geomagnetic field absence reduces adult body weight of a migratory insect by disrupting feeding behavior and appetite regulation

2019 ◽  
Author(s):  
Guijun Wan ◽  
Shoulin Jiang ◽  
Ming Zhang ◽  
Jingyu Zhao ◽  
Yingchao Zhang ◽  
...  
Keyword(s):  
Body Weight ◽  
Feeding Behavior ◽  
Geomagnetic Field ◽  
Expression Patterns ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Zero Magnetic Field ◽  
Food Aversion ◽  
Glucose Levels ◽  
Reduced Body

AbstractThe geomagnetic field (GMF) is well documented for its essential role as a cue used in animal orientation or navigation. Recent evidence indicates that the absence of GMF can trigger stress-like responses such as reduced body weight, as we have previously shown in newly emerged adults of the brown planthopper, Nilaparvata lugens. To test the hypothesis that reduced feeding in the absence of the GMF leads to a decrease of N. lugens body weight, we compared magnetic responses in feeding behavior, glucose levels, and expression of magnetoreception- and appetite-related genes in brown planthopper nymphs exposed to either a near-zero magnetic field (NZMF, i.e., GMF absence) or typical GMF conditions. In addition to observing the expected responses in the expression of the potential magnetosensor cryptochromes, the food intake of 5th instar nymphs was significantly reduced in insects reared in the absence of GMF. Insects that exhibited reduced feeding reared in the absence of the GMF also had higher glucose levels which is associated with food aversion. Expression patterns of appetite-related neuropeptide genes were also altered in the absence of GMF in a manner consistent with diminishing appetite. These findings support the hypothesis that strong changes in GMF intensity can affect insect feeding behavior and underlying regulatory processes. Our results provide further evidence that magnetoreception and regulatory responses to GMF changes can affect a wide variety of biological processes.

Influence of water-stressed rice on feeding behavior of brown planthopper, Nilaparvata lugens (Stål)

2017 ◽  
Vol 20 (2) ◽  
pp. 665-670 ◽  
Author(s):  
Ye Tan ◽  
Mufei Zhu ◽  
Wenyan Xu ◽  
Wenwu Zhou ◽  
Dongdong Lu ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Influence Of Water ◽  
Nilaparvata Lugens Stål
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