scholarly journals Large-Scale Production of Human iPSC-Derived Macrophages for Drug Screening

2020 ◽  
Vol 21 (13) ◽  
pp. 4808 ◽  
Author(s):  
Simon Gutbier ◽  
Florian Wanke ◽  
Nadine Dahm ◽  
Anna Rümmelin ◽  
Silke Zimmermann ◽  
...  
Keyword(s):  
Drug Screening ◽  
Large Scale ◽  
Neoplastic Cell ◽  
Leukemic Cells ◽  
High Yield ◽  
Scale Production ◽  
Large Scale Production ◽  
Compound Screening ◽  
Health And Disease ◽  
Induced Pluripotent

Tissue-resident macrophages are key players in inflammatory processes, and their activation and functionality are crucial in health and disease. Numerous diseases are associated with alterations in homeostasis or dysregulation of the innate immune system, including allergic reactions, autoimmune diseases, and cancer. Macrophages are a prime target for drug discovery due to their major regulatory role in health and disease. Currently, the main sources of macrophages used for therapeutic compound screening are primary cells isolated from blood or tissue or immortalized or neoplastic cell lines (e.g., THP-1). Here, we describe an improved method to employ induced pluripotent stem cells (iPSCs) for the high-yield, large-scale production of cells resembling tissue-resident macrophages. For this, iPSC-derived macrophage-like cells are thoroughly characterized to confirm their cell identity and thus their suitability for drug screening purposes. These iPSC-derived macrophages show strong cellular identity with primary macrophages and recapitulate key functional characteristics, including cytokine release, phagocytosis, and chemotaxis. Furthermore, we demonstrate that genetic modifications can be readily introduced at the macrophage-like progenitor stage in order to interrogate drug target-relevant pathways. In summary, this novel method overcomes previous shortcomings with primary and leukemic cells and facilitates large-scale production of genetically modified iPSC-derived macrophages for drug screening applications.

scholarly journals Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Alejandro Torres-Haro ◽  
Jorge Verdín ◽  
Manuel R. Kirchmayr ◽  
Melchor Arellano-Plaza
Keyword(s):  
Metabolic Engineering ◽  
Large Scale ◽  
Dry Mass ◽  
High Yield ◽  
Xanthophyllomyces Dendrorhous ◽  
Scale Production ◽  
Proteomic Data ◽  
Large Scale Production ◽  
Mutant Strains ◽  
Pharmaceutical Industries

AbstractAstaxanthin is a carotenoid with a number of assets useful for the food, cosmetic and pharmaceutical industries. Nowadays, it is mainly produced by chemical synthesis. However, the process leads to an enantiomeric mixture where the biologically assimilable forms (3R, 3′R or 3S, 3′S) are a minority. Microbial production of (3R, 3′R) astaxanthin by Xanthophyllomyces dendrorhous is an appealing alternative due to its fast growth rate and easy large-scale production. In order to increase X. dendrorhous astaxanthin yields, random mutant strains able to produce from 6 to 10 mg/g dry mass have been generated; nevertheless, they often are unstable. On the other hand, site-directed mutant strains have also been obtained, but they increase only the yield of non-astaxanthin carotenoids. In this review, we insightfully analyze the metabolic carbon flow converging in astaxanthin biosynthesis and, by integrating the biological features of X. dendrorhous with available metabolic, genomic, transcriptomic, and proteomic data, as well as the knowledge gained with random and site-directed mutants that lead to increased carotenoids yield, we propose new metabolic engineering targets to increase astaxanthin biosynthesis.

scholarly journals Fabrication of Nanosize ZnO and Zn1−xFexO Powder for Infrared Absorption by Flame Aerosol Synthesis

Crystals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 904
Author(s):  
Hui Tian ◽  
Lei Wang ◽  
Taisheng Yang ◽  
Zili Zhang
Keyword(s):  
Infrared Absorption ◽  
Large Scale ◽  
Low Cost ◽  
High Yield ◽  
Synthesis Methods ◽  
Scale Production ◽  
Absorption Properties ◽  
Aerosol Synthesis ◽  
Large Scale Production ◽  
Significant Difference

In this study, nanosized ZnO and Zn1−xFexO powders were synthesized using the flame aerosol synthesis (FAS) method. The microstructure of the ZnO powder shows a significant difference with different precursor concentrations. By adding Fe to the precursor, nanosized Zn1−xFexO powder (x = 0~0.1) can be easily fabricated. The phase formation, microstructure, and infrared absorption properties were systematically investigated by XRD, SEM, TEM, and IR. With the substitution of Fe into the Zn site, lattice distortion occurred, resulting in excellent infrared absorption properties. Compared to other conventional synthesis methods, the FAS method has the advantages of high yield, high crystallinity, and low cost; furthermore, nanosized powder is easily obtained. The FAS method is believed to be one of the best choices for the large-scale production of ZnO and Zn1−xFexO powders.

scholarly journals Anaerobic Solid-State Fermentation of Soybean Meal With Bacillus sp. to Improve Nutritional Quality

2021 ◽  
Vol 8 ◽  
Author(s):  
Yanhui Yao ◽  
Hongya Li ◽  
Jia Li ◽  
Baocheng Zhu ◽  
Tongguo Gao
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Nutritional Quality ◽  
Large Scale ◽  
High Yield ◽  
Scale Production ◽  
Large Scale Production ◽  
The Impact

The study evaluated the impact of fermentation with Bacillus sp. on the nutritional quality of soybean meal (SBM) and the changes of bacterial community structure during fermentation. High protease-producing strains were screened to degrade SBM macromolecular protein and anti-nutritional factors (ANFs). Unsterilized SBM then underwent an anaerobic solid-state fermentation method to evaluate the effects of fermentation. Results showed that for the nine high-producing protease strains that were screened, acid-soluble protein (ASP) contents in fermented SBM increased, with the highest value found to be 13.48%, which was fermented using strain N-11. N-11 was identified as Bacillus subtilis. N-11 fermentation reduced ANFs such as glycinin and β-conglycinin by 82.38 and 88.32%, respectively. During N-11 fermentation, the bacterial richness and diversity in SBM increased but not significantly. The high-yield protease strain B. subtilis N-11 selected in this experiment improved the nutritional quality of SBM through fermentation, and it can be used for industrial large-scale production.

scholarly journals Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2744 ◽  
Author(s):  
San-Lang Wang ◽  
Van Bon Nguyen ◽  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
Minh Trung Nguyen ◽  
...  
Keyword(s):  
Large Scale ◽  
Proteolytic Enzymes ◽  
Culture Conditions ◽  
High Yield ◽  
Scale Production ◽  
Pharmaceutical Drugs ◽  
Microbial Conversion ◽  
N Sources ◽  
Large Scale Production ◽  
Potential Applications

The technology of microbial conversion provides a potential way to exploit compounds of biotechnological potential. The red pigment prodigiosin (PG) and other PG-like pigments from bacteria, majorly from Serratia marcescens, have been reported as bioactive secondary metabolites that can be used in the broad fields of agriculture, fine chemicals, and pharmacy. Increasing PG productivity by investigating the culture conditions especially the inexpensive carbon and nitrogen (C/N) sources has become an important factor for large-scale production. Investigations into the bioactivities and applications of PG and its related compounds have also been given increased attention. To save production cost, chitin and protein-containing fishery byproducts have recently been investigated as the sole C/N source for the production of PG and chitinolytic/proteolytic enzymes. This strategy provides an environmentally-friendly selection using inexpensive C/N sources to produce a high yield of PG together with chitinolytic and proteolytic enzymes by S. marcescens. The review article will provide effective references for production, bioactivity, and application of S. marcescens PG in various fields such as biocontrol agents and potential pharmaceutical drugs.

High-yield amplification ofCryptosporidium parvumin interferon γ receptor knockout mice

Parasitology ◽  
2008 ◽  
Vol 135 (10) ◽  
pp. 1151-1156 ◽  
Author(s):  
J. von OETTINGEN ◽  
M. NATH-CHOWDHURY ◽  
B. J. WARD ◽  
A. C. RODLOFF ◽  
M. J. ARROWOOD ◽  
...  
Keyword(s):  
Large Scale ◽  
Interferon Γ ◽  
High Yield ◽  
Scale Production ◽  
Large Scale Production ◽  
Ether Extraction ◽  
External Sources ◽  
And Storage

SUMMARYTo date, large-scale production ofCryptosporidium parvumoocysts has only been achieved by amplification in neonatal calves and sheep. Many laboratories currently depend on supplies from external sources and store oocysts for prolonged periods which results in progressive loss of viability. Six to 8-week-old interferon γ receptor knockout (IFNγR-KO) mice on a C57BL/6 background were inoculated by gavage (2000 oocysts/animal). Fecal pellets were collected daily from 7 days post-infection (p.i.) up to 2 weeks p.i. Intestinal oocyst yield was assessed at days 11, 12 and 14 p.i. by homogenization of intestinal tissues. Ether extraction and one or more NaCl flotations were used to purify oocysts. Total recoveries averaged 2·6×106oocysts/mouse from fecal material and 3·8×107oocysts/mouse from intestinal tissues. Overall, 2·3×109purified oocysts were obtained from 60 mice. Recovered oocysts were capable of sporulation and were shown to be infectious bothin vitroandin vivo. Oocyst amplification was achieved in only 11–14 days with minimal expense. The simplicity of this method presents a practical alternative for the routine passage, maintenance and storage ofC. parvumin biomedical laboratories.

Large-scale production of ultrathin carbon nitride-based photocatalysts for high-yield hydrogen evolution

2021 ◽  
Vol 281 ◽  
pp. 119475 ◽  
Author(s):  
Jianjian Yi ◽  
Ting Fei ◽  
Li Li ◽  
Qing Yu ◽  
Sai Zhang ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
Large Scale ◽  
Carbon Nitride ◽  
High Yield ◽  
Scale Production ◽  
Large Scale Production

Factor VIII With Monophasic Degradation, Produced by a New Fastthawing Method

1979 ◽  
Author(s):  
M. Ezoan ◽  
J.F. Hansen ◽  
J. Gormsen
Keyword(s):  
Factor Viii ◽  
Large Scale ◽  
High Yield ◽  
Scale Production ◽  
Large Scale Production ◽  
Freeze Dried ◽  
Labile Component ◽  
Small Pool ◽  
One Year

A new method has been developed for the large-scale production of cryopre-cipitate with a high yield of factor VIII. The freeze-dried factor VIII product has a solubility comparable to that of intermediate purified products and snows a remarkable in vivo stability. These improvements have been mainly accomplished by using a fast thawing method based on energy transfer through microwaves. Deep-frozen plasma bags (200 ml) are thawed in less than 10 min. with the temperature no where exceeding 4°C. In our routine production of freeze-dried, small-pool cryoprecipitate the yield of factor VIII is about 450 Units/liter of plasma. The concentration of the dissolved product is high being at least 2.0 Units/ml. This factor VIII concentrate has been used for home treatment for about one year, Clinical studies have snown a mean factor VIII recovery of 99.7% and a loss in activity of only 25% during the first 5 hours. The degradation follows a single exponential decay with time. This result indicates the absence of a labile component of factor VI VIII, which is usually observed along with the more stable component.

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