scholarly journals A Passive Microfluidic Device Based on Crossflow Filtration for Cell Separation Measurements: A Spectrophotometric Characterization

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 125 ◽  
Author(s):  
Vera Faustino ◽  
Susana Catarino ◽  
Diana Pinho ◽  
Rui Lima ◽  
Graça Minas
Keyword(s):  
Optical Absorption ◽  
Microfluidic Device ◽  
Cell Separation ◽  
Single Step ◽  
Crossflow Filtration ◽  
Chemically Modified ◽  
Key Factor ◽  
Infected Rbcs ◽  
Passive Separation ◽  
Diagnostic Applications

Microfluidic devices have been widely used as a valuable research tool for diagnostic applications. Particularly, they have been related to the successful detection of different diseases and conditions by assessing the mechanical properties of red blood cells (RBCs). Detecting deformability changes in the cells and being able to separate those cells may be a key factor in assuring the success of detection of some blood diseases with diagnostic devices. To detect and separate the chemically modified RBCs (mimicking disease-infected RBCs) from healthy RBCs, the present work proposes a microfluidic device comprising a sequence of pillars with different gaps and nine different outlets used to evaluate the efficiency of the device by measuring the optical absorption of the collected samples. This latter measurement technique was tested to distinguish between healthy RBCs and RBCs chemically modified with glutaraldehyde. The present study indicates that it was possible to detect a slight differences between the samples using an optical absorption spectrophotometric setup. Hence, the proposed microfluidic device has the potential to perform in one single step a partial passive separation of RBCs based on their deformability.

scholarly journals Differential Spleen Remodeling Associated with Different Levels of Parasite Virulence Controls Disease Outcome in Malaria Parasite Infections

mSphere ◽  
2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Ximei Huang ◽  
Sha Huang ◽  
Lai Chun Ong ◽  
Jason Chu-Shern Lim ◽  
Rebecca Joan Mary Hurst ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Malaria Parasite ◽  
Parasite Clearance ◽  
Biomechanical Properties ◽  
Parasite Infection ◽  
Complex Interaction ◽  
Content Type ◽  
Key Factor ◽  
Infected Rbcs ◽  
Rbc Deformability

ABSTRACT The spleen and its response to parasite infection are important in eliminating parasites in malaria. By comparing P. yoelii parasite lines with different disease outcomes in mice that had either intact spleens or had had their spleens removed, we showed that upon parasite infection, the spleen exhibits dramatic changes that can affect parasite clearance. The spleen itself directly impacts RBC deformability independently of parasite genetics. The data indicated that the changes in the biomechanical properties of malaria parasite-infected RBCs are the result of the complex interaction between host and parasite, and RBC deformability itself can serve as a novel predictor of clinical outcome. The results also suggest that early responses in the spleen are a key factor directing the clinical outcome of an infection. Infections by malaria parasites can lead to very different clinical outcomes, ranging from mild symptoms to death. Differences in the ability of the spleen to deal with the infected red blood cells (iRBCs) are linked to differences in virulence. Using virulent and avirulent strains of the rodent malaria parasite Plasmodium yoelii, we investigated how parasite virulence modulates overall spleen function. Following parasite invasion, a difference in parasite virulence was observed in association with different levels of spleen morphology and iRBC rigidity, both of which contributed to enhanced parasite clearance. Moreover, iRBC rigidity as modulated by the spleen was demonstrated to correlate with disease outcome and thus can be used as a robust indicator of virulence. The data indicate that alterations in the biomechanical properties of iRBCs are the result of the complex interaction between host and parasite. Furthermore, we confirmed that early spleen responses are a key factor in directing the clinical outcome of an infection. IMPORTANCE The spleen and its response to parasite infection are important in eliminating parasites in malaria. By comparing P. yoelii parasite lines with different disease outcomes in mice that had either intact spleens or had had their spleens removed, we showed that upon parasite infection, the spleen exhibits dramatic changes that can affect parasite clearance. The spleen itself directly impacts RBC deformability independently of parasite genetics. The data indicated that the changes in the biomechanical properties of malaria parasite-infected RBCs are the result of the complex interaction between host and parasite, and RBC deformability itself can serve as a novel predictor of clinical outcome. The results also suggest that early responses in the spleen are a key factor directing the clinical outcome of an infection.

scholarly journals Applications of Dendrimers in Drug Delivery Agents, Diagnosis, Therapy, and Detection

2014 ◽  
Vol 2014 ◽  
pp. 1-19 ◽  
Author(s):  
B. Noriega-Luna ◽  
Luis A. Godínez ◽  
Francisco J. Rodríguez ◽  
A. Rodríguez ◽  
G. Zaldívar-Lelo de Larrea ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Three Dimensional ◽  
Modified Electrodes ◽  
Biologically Active ◽  
Protein Mimics ◽  
Chemically Modified ◽  
Biological Compatibility ◽  
Variable Chemical ◽  
Diagnostic Applications ◽  
Diagnosis Therapy

In recent years, the application of dendrimers in biomedicine attracted much attention from scientists. Dendrimers are interesting for biomedical applications because of their characteristics, including: a hyperbranching, well-defined globular structures, excellent structural uniformity, multivalency, variable chemical composition, and high biological compatibility. In particular, the three-dimensional architecture of dendrimers can incorporate a variety of biologically active agents to form biologically active conjugates. This review of dendrimers focuses on their use as protein mimics, drug delivery agents, anticancer and antiviral therapeutics, and in biomedical diagnostic applications such as chemically modified electrodes.

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

Cell separation by an aqueous two-phase system in a microfluidic device

The Analyst ◽  
2009 ◽  
Vol 134 (10) ◽  
pp. 1994 ◽  
Author(s):  
Masatoshi Tsukamoto ◽  
Shu Taira ◽  
Shohei Yamamura ◽  
Yasutaka Morita ◽  
Naoki Nagatani ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Cell Separation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Deformability and size-based cancer cell separation using an integrated microfluidic device

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7335-7346 ◽  
Author(s):  
Long Pang ◽  
Shaofei Shen ◽  
Chao Ma ◽  
Tongtong Ma ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Cell Size ◽  
Cancer Cell ◽  
Microfluidic Device ◽  
Cell Separation

We present an integrated microfluidic device for cell separation based on the cell size and deformability by combining the microstructure-constricted filtration and pneumatic microvalves.

An inertial microfluidic device for targeted cell separation

2018 ◽  
Vol 44 ◽  
pp. S129
Author(s):  
A. Shamloo ◽  
A. Mashhadian
Keyword(s):  
Microfluidic Device ◽  
Cell Separation

Tunnel dielectrophoresis for ultra-high precision size-based cell separation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yu-Chun Kung ◽  
Kayvan R. Niazi ◽  
Pei-Yu Chiou
Keyword(s):  
Cell Size ◽  
High Precision ◽  
Microfluidic Device ◽  
Cell Separation ◽  
Label Free

In this study, we present a microfluidic device that can achieve label-free and size-based cell separation with high size differential resolution for arbitrary cell size band filtering.

High-throughput rare cell separation from blood samples using steric hindrance and inertial microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2525-2538 ◽  
Author(s):  
Shaofei Shen ◽  
Chao Ma ◽  
Lei Zhao ◽  
Yaolei Wang ◽  
Jian-Chun Wang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Steric Hindrance ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Blood Samples ◽  
Rare Cells

We present a multistage microfluidic device for continuous label-free separation of rare cells using a combination of inertial microfluidics and steric hindrance.

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