High-throughput rare cell separation from blood samples using steric hindrance and inertial microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2525-2538 ◽  
Author(s):  
Shaofei Shen ◽  
Chao Ma ◽  
Lei Zhao ◽  
Yaolei Wang ◽  
Jian-Chun Wang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Steric Hindrance ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Blood Samples ◽  
Rare Cells

We present a multistage microfluidic device for continuous label-free separation of rare cells using a combination of inertial microfluidics and steric hindrance.

Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation

Lab on a Chip ◽  
2011 ◽  
Vol 11 (11) ◽  
pp. 1870 ◽  
Author(s):  
Ali Asgar S. Bhagat ◽  
Han Wei Hou ◽  
Leon D. Li ◽  
Chwee Teck Lim ◽  
Jongyoon Han
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Cell Separation ◽  
Inertial Microfluidics

Tunnel dielectrophoresis for ultra-high precision size-based cell separation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yu-Chun Kung ◽  
Kayvan R. Niazi ◽  
Pei-Yu Chiou
Keyword(s):  
Cell Size ◽  
High Precision ◽  
Microfluidic Device ◽  
Cell Separation ◽  
Label Free

In this study, we present a microfluidic device that can achieve label-free and size-based cell separation with high size differential resolution for arbitrary cell size band filtering.

Label-Free Cancer Cell Separation from Human Whole Blood Using Inertial Microfluidics at Low Shear Stress

2013 ◽  
Vol 85 (13) ◽  
pp. 6213-6218 ◽  
Author(s):  
Myung Gwon Lee ◽  
Joong Ho Shin ◽  
Chae Yun Bae ◽  
Sungyoung Choi ◽  
Je-Kyun Park
Keyword(s):  
Shear Stress ◽  
Cancer Cell ◽  
Whole Blood ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Human Whole Blood ◽  
Free Cancer Cell ◽  
Low Shear Stress ◽  
Low Shear

scholarly journals A microfluidic device for label-free isolation of tumor cell clusters from unprocessed blood samples

2019 ◽  
Vol 13 (4) ◽  
pp. 044111 ◽  
Author(s):  
Nabiollah Kamyabi ◽  
Jonathan Huang ◽  
Jaewon J. Lee ◽  
Vincent Bernard ◽  
Alexander Semaan ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Microfluidic Device ◽  
Label Free ◽  
Blood Samples ◽  
Cell Clusters

Automated Microfluidic Instrument for Label-Free and High-Throughput Cell Separation

2018 ◽  
Vol 90 (6) ◽  
pp. 4212-4220 ◽  
Author(s):  
Xinjie Zhang ◽  
Zhixian Zhu ◽  
Nan Xiang ◽  
Feifei Long ◽  
Zhonghua Ni
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Label Free

scholarly journals High resolution and high throughput bacteria separation from blood using elasto-inertial microfluidics

2020 ◽  
Author(s):  
Sharath Narayana Iyengar ◽  
Tharagan Kumar ◽  
Gustaf Mårtensson ◽  
Aman Russom
Keyword(s):  
Sample Preparation ◽  
Blood Sample ◽  
High Throughput ◽  
Blood Cells ◽  
Separation Efficiency ◽  
Unmet Need ◽  
Outer Wall ◽  
Separation Performance ◽  
Label Free ◽  
Inertial Microfluidics

AbstractImproved sample preparation has the potential to address a huge unmet need for fast turnaround sepsis tests that enable early administration of appropriate antimicrobial therapy. In recent years, inertial and elasto-inertial microfluidics-based sample preparation has gained substantial interest for bioparticle separation applications. However, for applications in blood stream infections the throughput and bacteria separation efficiency has thus far been limited. In this work, for the first time we report elasto-inertial microfluidics-based bacteria isolation from blood at throughputs and efficiencies unparalleled with current microfluidics-based state of the art. In the method, bacteria-spiked blood sample is prepositioned close to the outer wall of a spiral microchannel using a viscoelastic sheath buffer. The blood cells will remain fully focused throughout the length of the channel while bacteria migrate to the inner wall for effective separation. Initially, particles of different sizes were used to investigate particle focusing and the separation performance of the spiral device. A separation efficiency of 96% for the 1 µm particles was achieved, while 100% of 3 µm particles were recovered at the desired outlet at a high throughput of 1 mL/min. Following, processing blood samples revealed a minimum of 1:2 dilution was necessary to keep the blood cells fully focus at the outer wall. In experiments involving bacteria spiked in diluted blood, viable E.coli were continuously separated at a total flow rate of 1 mL/min, with an efficiency between 82 to 90% depending on the blood dilution. Using a single spiral, it takes 40 minutes to process 1 mL of blood at a separation efficiency of 82% and 3 hours at 90% efficiency. To the best of our knowledge, this is the highest blood sample throughput per single microfluidic chip reported for the corresponding separation efficiency. As such, the label-free, passive and high throughput bacteria isolation method has a great potential for speeding up downstream phenotypic and molecular analysis of bacteria.

Label-free inertial-ferrohydrodynamic cell separation with high throughput and resolution

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Alicia G Puig ◽  
Jamie Hodgson ◽  
...  
Keyword(s):  
High Throughput ◽  
Biological Samples ◽  
Cell Separation ◽  
Target Cells ◽  
Label Free ◽  
Advanced Technologies ◽  
Disease Diagnostics

Rapid and label-free separation of target cells in biological samples provided unique opportunity for disease diagnostics and treatment. However, even with advanced technologies for cell separation, the limiting throughput, high...

High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Microfluidics and Membrane

2016 ◽  
Author(s):  
Jun Zhang ◽  
Sheng Yan ◽  
Dan Yuan ◽  
Gursel Alici ◽  
Nam-trung Nguyen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Microfluidic Device ◽  
High Throughput ◽  
Membrane Filtration ◽  
Membrane Filter ◽  
Inertial Microfluidics ◽  
High Efficient ◽  
Integrated Device ◽  
Plasma Extraction

Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction.

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