Deformability and size-based cancer cell separation using an integrated microfluidic device

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7335-7346 ◽  
Author(s):  
Long Pang ◽  
Shaofei Shen ◽  
Chao Ma ◽  
Tongtong Ma ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Cell Size ◽  
Cancer Cell ◽  
Microfluidic Device ◽  
Cell Separation

We present an integrated microfluidic device for cell separation based on the cell size and deformability by combining the microstructure-constricted filtration and pneumatic microvalves.

Tunnel dielectrophoresis for ultra-high precision size-based cell separation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yu-Chun Kung ◽  
Kayvan R. Niazi ◽  
Pei-Yu Chiou
Keyword(s):  
Cell Size ◽  
High Precision ◽  
Microfluidic Device ◽  
Cell Separation ◽  
Label Free

In this study, we present a microfluidic device that can achieve label-free and size-based cell separation with high size differential resolution for arbitrary cell size band filtering.

scholarly journals In vivo imaging of cancer cell size and cellularity using temporal diffusion spectroscopy

2016 ◽  
Vol 78 (1) ◽  
pp. 156-164 ◽  
Author(s):  
Xiaoyu Jiang ◽  
Hua Li ◽  
Jingping Xie ◽  
Eliot T. McKinley ◽  
Ping Zhao ◽  
...  
Keyword(s):  
Cell Size ◽  
Cancer Cell ◽  
In Vivo Imaging

PDMS micropillar-based microchip for efficient cancer cell capture

RSC Advances ◽  
2015 ◽  
Vol 5 (64) ◽  
pp. 52161-52166 ◽  
Author(s):  
Jingrong Xiao ◽  
Weiqi He ◽  
Zhengtao Zhang ◽  
Weiying Zhang ◽  
Yiping Cao ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Microfluidic Device ◽  
Cell Capture

We introduce a micropillar-based microfluidic device for efficient and rapid cancer cell capture.

Abstract 3480: High-throughput size and deformability-based cancer cell separation

2014 ◽  
Author(s):  
Gordon Yip ◽  
Daniel Ionescu ◽  
Edwin Johnson ◽  
Mikael Dick ◽  
Zecong Fang ◽  
...  
Keyword(s):  
Cancer Cell ◽  
High Throughput ◽  
Cell Separation

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

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