Tunnel dielectrophoresis for ultra-high precision size-based cell separation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yu-Chun Kung ◽  
Kayvan R. Niazi ◽  
Pei-Yu Chiou
Keyword(s):  
Cell Size ◽  
High Precision ◽  
Microfluidic Device ◽  
Cell Separation ◽  
Label Free

In this study, we present a microfluidic device that can achieve label-free and size-based cell separation with high size differential resolution for arbitrary cell size band filtering.

Deformability and size-based cancer cell separation using an integrated microfluidic device

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7335-7346 ◽  
Author(s):  
Long Pang ◽  
Shaofei Shen ◽  
Chao Ma ◽  
Tongtong Ma ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Cell Size ◽  
Cancer Cell ◽  
Microfluidic Device ◽  
Cell Separation

We present an integrated microfluidic device for cell separation based on the cell size and deformability by combining the microstructure-constricted filtration and pneumatic microvalves.

High-throughput rare cell separation from blood samples using steric hindrance and inertial microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2525-2538 ◽  
Author(s):  
Shaofei Shen ◽  
Chao Ma ◽  
Lei Zhao ◽  
Yaolei Wang ◽  
Jian-Chun Wang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Steric Hindrance ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Blood Samples ◽  
Rare Cells

We present a multistage microfluidic device for continuous label-free separation of rare cells using a combination of inertial microfluidics and steric hindrance.

scholarly journals High-Sensitivity in Dielectrophoresis Separations

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 391
Author(s):  
Benjamin G. Hawkins ◽  
Nelson Lai ◽  
David S. Clague
Keyword(s):  
Electrical Properties ◽  
Cell Size ◽  
Electric Fields ◽  
Cell Separation ◽  
High Sensitivity ◽  
Label Free ◽  
Phenotypic Differences ◽  
Actuation Force ◽  
Highly Sensitive ◽  
Phenotypic Variations

The applications of dielectrophoretic (DEP) techniques for the manipulation of cells in a label-free fashion within microfluidic systems continue to grow. However, a limited number of methods exist for making highly sensitive separations that can isolate subtle phenotypic differences within a population of cells. This paper explores efforts to leverage that most compelling aspect of DEP—an actuation force that depends on particle electrical properties—in the background of phenotypic variations in cell size. Several promising approaches, centering around the application of multiple electric fields with spatially mapped magnitude and/or frequencies, are expanding the capability of DEP cell separation.

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

scholarly journals Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays

2007 ◽  
Vol 53 (7) ◽  
pp. 1323-1329 ◽  
Author(s):  
Dianping Tang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Tumor Markers ◽  
Microfluidic Device ◽  
Simultaneous Detection ◽  
Reference Electrode ◽  
Cost Effective ◽  
Sio2 Nanoparticles ◽  
Magnetic Bead ◽  
Label Free ◽  
Specific Tumor ◽  
Protein Diagnostics

Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was <0.5 μg/L (or <0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were <4.5% and 8.7% for analyte concentrations >5 μg/L (or >5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was <10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

scholarly journals Label-Free Virus Capture and Release by a Microfluidic Device Integrated with Porous Silicon Nanowire Forest

Small ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 1603135 ◽  
Author(s):  
Yiqiu Xia ◽  
Yi Tang ◽  
Xu Yu ◽  
Yuan Wan ◽  
Yizhu Chen ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Microfluidic Device ◽  
Silicon Nanowire ◽  
Label Free ◽  
Free Virus ◽  
Capture And Release ◽  
Porous Silicon Nanowire

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

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