scholarly journals Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry

2019 ◽  
Vol 9 (22) ◽  
pp. 4789
Author(s):  
Raul Nicoară ◽  
Maria Ilieș ◽  
Alina Uifălean ◽  
Cristina Adela Iuga ◽  
Felicia Loghin
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Protein Corona ◽  
Nanoparticle Size ◽  
Full Picture ◽  
Model Protein ◽  
Modified Surface ◽  
Living Organisms ◽  
The Impact ◽  
Biological Milieu

The interactions of nanoparticles with living organisms are driven by an interface called the protein corona. This interface is formed when nanoparticles are introduced in biological milieu and proteins are adsorbed at nanoparticles’ surfaces. Understanding the factors that are responsible for the formation and the composition of the protein corona could reveal mechanistic insights that are involved in the interaction of nanoparticles with biological structures. Multiple studies have qualitatively described the protein corona, but just a few have proposed quantification methods, especially for gold nanoparticles. Using bovine serum albumin conjugated with fluorescein-5-isothiocyanate as a model protein, we developed a fluorescent-based quantification method for gold nanoparticles’ protein coronas. The impact of nanoparticle size and surface chemistry was studied, and our research emphasizes that size and surface chemistry are determinant factors: Bigger nanoparticles and amino-modified surface chemistry are responsible for higher protein adsorption compared to smaller ones and carboxyl- or methoxy-modified surface chemistry. The proposed method can be used to complete the full picture of the interactions of nanoparticles with biological milieu and to describe the parameters which govern these interactions for the better development of nanomedicines.

scholarly journals Biointeractions of Ultrasmall Gold Nanoparticles: Influence of Nanoparticle Size and Surface Chemistry

2016 ◽  
Vol 110 (3) ◽  
pp. 530a
Author(s):  
Luiza L. Knittel ◽  
Sergio A. Hassan ◽  
Maria A. Aronova ◽  
Peter Schuck ◽  
Richard D. Leapman ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Nanoparticle Size

Adsorption of ozone and plasmonic properties of gold hydrosol: the effect of the nanoparticle size

2015 ◽  
Vol 17 (28) ◽  
pp. 18431-18436 ◽  
Author(s):  
Boris G. Ershov ◽  
Evgeny V. Abkhalimov ◽  
Vyacheslav I. Roldughin ◽  
Viktor M. Rudoy ◽  
Olga V. Dement'eva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Plasmon Resonance ◽  
Bathochromic Shift ◽  
Resonance Peak ◽  
Nanoparticle Size ◽  
Plasmon Resonance Peak ◽  
The Impact

The impact of the size of gold nanoparticles on the magnitude of the bathochromic shift of their plasmon resonance peak upon ozone adsorption is revealed and analyzed.

The Impact of Surface Chemistry on the Interfacial Evaporation-Driven Self-Assembly of Thermoplasmonic Gold Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Feiyu Zheng ◽  
Yingyue Zhang ◽  
Liuchang Dong ◽  
Dengwu Zhao ◽  
Rui Feng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Self Assembly ◽  
Different Types ◽  
The Impact

This paper reports an interfacial evaporation-driven approach for self-assembly of gold nanoparticles (AuNPs) film at the interface of liquid/air. We have designed colloidal plasmonic AuNPs capped with different types and...

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

2017 ◽  
Vol 27 (42) ◽  
pp. 1701956 ◽  
Author(s):  
Blair D. Johnston ◽  
Wolfgang G. Kreyling ◽  
Christian Pfeiffer ◽  
Martin Schäffler ◽  
Hakan Sarioglu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Key Factors

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

scholarly journals Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 780
Author(s):  
Krystyna Makowska ◽  
Slawomir Gonkowski
Keyword(s):  
Bisphenol A ◽  
Endocrine Disruptor ◽  
Sympathetic Innervation ◽  
Daily Intake ◽  
Everyday Objects ◽  
Living Organisms ◽  
The Impact ◽  
Heart Wall ◽  
Cholinergic Structures

Bisphenol A (BPA) contained in plastics used in the production of various everyday objects may leach from these items and contaminate food, water and air. As an endocrine disruptor, BPA negatively affects many internal organs and systems. Exposure to BPA also contributes to heart and cardiovascular system dysfunction, but many aspects connected with this activity remain unknown. Therefore, this study aimed to investigate the impact of BPA in a dose of 0.05 mg/kg body weight/day (in many countries such a dose is regarded as a tolerable daily intake–TDI dose of BPA–completely safe for living organisms) on the neurochemical characterization of nerves located in the heart wall using the immunofluorescence technique. The obtained results indicate that BPA (even in such a relatively low dose) increases the number of nerves immunoreactive to neuropeptide Y, substance P and tyrosine hydroxylase (used here as a marker of sympathetic innervation). However, BPA did not change the number of nerves immunoreactive to vesicular acetylcholine transporter (used here as a marker of cholinergic structures). These observations suggest that changes in the heart innervation may be at the root of BPA-induced circulatory disturbances, as well as arrhythmogenic and/or proinflammatory effects of this endocrine disruptor. Moreover, changes in the neurochemical characterization of nerves in the heart wall may be the first sign of exposure to BPA.

scholarly journals Films and Materials Derived from Aminomalononitrile

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 82
Author(s):  
Helmut Thissen ◽  
Richard A. Evans ◽  
Vincent Ball
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
State Of The Art ◽  
Chemical Reactivity ◽  
The State ◽  
Deposition Kinetics ◽  
Conformal Coating ◽  
Early Atmosphere ◽  
Living Organisms ◽  
Hypothetical Structure

In recent years major advances in surface chemistry and surface functionalization have been performed through the development, most often inspired by living organisms, of versatile methodologies. Among those, the contact of substrates with aminomalononitrile (AMN) containing solutions at pH = 8.5 allows a conformal coating to be deposited on the surface of all known classes of material. Since AMN is a molecule probably formed in the early atmosphere of our planet and since HCN-based compounds have been detected on many comets and Titan (Saturn’s largest moon) it is likely that such molecules will open a large avenue in surface functionalization mostly for bio-applications. This mini review describes the state of the art of AMN-based coatings from their deposition kinetics, composition, chemical reactivity, hypothetical structure to their first applications as biomaterials. Finally, the AMN-based versatile coatings are compared to other kinds of versatile coating based on catecholamines and polyphenols.

The impact of protein corona on the biological behavior of targeting nanomedicines

2022 ◽  
pp. 121458
Author(s):  
Masoud Farshbaf ◽  
Hadi Valizadeh ◽  
Yunes Panahi ◽  
Yousef Fatahi ◽  
Meiwan Chen ◽  
...  
Keyword(s):  
Protein Corona ◽  
Biological Behavior ◽  
The Impact
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