Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

2017 ◽  
Vol 27 (42) ◽  
pp. 1701956 ◽  
Author(s):  
Blair D. Johnston ◽  
Wolfgang G. Kreyling ◽  
Christian Pfeiffer ◽  
Martin Schäffler ◽  
Hakan Sarioglu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Key Factors

A multi-coordinating polymer ligand optimized for the functionalization of metallic nanocrystals and nanorods

2016 ◽  
Vol 191 ◽  
pp. 481-494 ◽  
Author(s):  
Wentao Wang ◽  
Xin Ji ◽  
Hunter Burns ◽  
Hedi Mattoussi
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Growth Media ◽  
Nucleophilic Addition Reaction ◽  
One Step ◽  
Nonspecific Interactions ◽  
Anchoring Groups ◽  
Polymer Ligand

We report the design and use of a multi-coordinating polymer ligand that is ideally suited for functionalizing gold nanoparticles and nanorods, and promoting their steric stabilization in buffer media. The ligand is prepared via a one-step nucleophilic addition reaction between poly(isobutylene-alt-maleic anhydride) and amine-modified anchoring groups and hydrophilic moieties. Surface functionalization of gold nanoparticles and nanorods with this polymer yields nanocrystals that exhibit excellent long-term colloidal stability over a broad range of conditions, including pH changes and in growth media, as verified using dynamic light scattering measurements combined with agarose gel electrophoresis. This polymer coating can also prevent the formation of protein corona. These features bode well for use in biological applications where small size, reduced nonspecific interactions and colloidal stability are highly desired. Furthermore, this design can be easily expanded to functionalize a variety of other inorganic nanocrystals.

scholarly journals Reducing Protein Corona Formation and Enhancing Colloidal Stability of Gold Nanoparticles by Capping with Silica Monolayers

2018 ◽  
Vol 31 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Jesús Mosquera ◽  
Isabel García ◽  
Malou Henriksen-Lacey ◽  
Guillermo González-Rubio ◽  
Luis M. Liz-Marzán
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Corona Formation

scholarly journals Revealing the Nanoparticle-Protein Corona with a Solid-State Nanopore

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3524 ◽  
Author(s):  
Diego Coglitore ◽  
Pierre Eugene Coulon ◽  
Jean-Marc Janot ◽  
Sébastien Balme
Keyword(s):  
Gold Nanoparticles ◽  
Solid State ◽  
Protein Adsorption ◽  
Internal Energy ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Intrinsic Properties ◽  
Colloidal Properties ◽  
Salt Addition ◽  
Structural Category

Protein adsorption at the liquid–solid interface is an old but not totally solved topic. One challenge is to find an easy way to characterize the protein behavior on nanoparticles and make a correlation with its intrinsic properties. This work aims to investigate protein adsorption on gold nanoparticles and the colloidal properties. The protein panel was chosen from different structural categories (mainly-α, mainly-β or mix-αβ). The result shows that the colloidal stability with salt addition does not depend on the structural category. Conversely, using the single nanopore technique, we show that the mainly-α proteins form a smaller corona than the mainly-β proteins. We assign these observations to the lower internal energy of α-helices, making them more prone to form a homogeneous corona layer.

scholarly journals Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry

2019 ◽  
Vol 9 (22) ◽  
pp. 4789
Author(s):  
Raul Nicoară ◽  
Maria Ilieș ◽  
Alina Uifălean ◽  
Cristina Adela Iuga ◽  
Felicia Loghin
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Protein Corona ◽  
Nanoparticle Size ◽  
Full Picture ◽  
Model Protein ◽  
Modified Surface ◽  
Living Organisms ◽  
The Impact ◽  
Biological Milieu

The interactions of nanoparticles with living organisms are driven by an interface called the protein corona. This interface is formed when nanoparticles are introduced in biological milieu and proteins are adsorbed at nanoparticles’ surfaces. Understanding the factors that are responsible for the formation and the composition of the protein corona could reveal mechanistic insights that are involved in the interaction of nanoparticles with biological structures. Multiple studies have qualitatively described the protein corona, but just a few have proposed quantification methods, especially for gold nanoparticles. Using bovine serum albumin conjugated with fluorescein-5-isothiocyanate as a model protein, we developed a fluorescent-based quantification method for gold nanoparticles’ protein coronas. The impact of nanoparticle size and surface chemistry was studied, and our research emphasizes that size and surface chemistry are determinant factors: Bigger nanoparticles and amino-modified surface chemistry are responsible for higher protein adsorption compared to smaller ones and carboxyl- or methoxy-modified surface chemistry. The proposed method can be used to complete the full picture of the interactions of nanoparticles with biological milieu and to describe the parameters which govern these interactions for the better development of nanomedicines.

Seed-mediated synthesis and PEG coating of gold nanoparticles for controlling morphology and sizes

MRS Advances ◽  
2020 ◽  
Vol 5 (63) ◽  
pp. 3353-3360
Author(s):  
Susana Helena Arellano Ramírez ◽  
Perla García Casillas ◽  
Christian Chapa González
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Room Temperature ◽  
Colloidal Stability ◽  
Infrared Spectrometry ◽  
Ammonium Bromide ◽  
Chloroauric Acid ◽  
Vis Spectroscopy ◽  
Seed Mediated ◽  
Peg Coating

AbstractA significant area of research is biomedical applications of nanoparticles which involves efforts to control the physicochemical properties through simple and scalable processes. Gold nanoparticles have received considerable attention due to their unique properties that they exhibit based on their morphology. Gold nanospheres (AuNSs) and nanorods (AuNRs) were prepared with a seed-mediated method followed of polyethylene glycol (PEG)-coating. The seeds were prepared with 0.1 M cetyltrimethyl-ammonium bromide (CTAB), 0.005 M chloroauric acid (HAuCl4), and 0.01 M sodium borohydride (NaBH4) solution. Gold nanoparticles with spherical morphology was achieved by growth by aggregation at room temperature, while to achieve the rod morphology 0.1 M silver nitrate (AgNO3) and 0.1 M ascorbic acid solution were added. The gold nanoparticles obtained by the seed-mediated synthesis have spherical or rod shapes, depending on the experimental conditions, and a uniform particle size. Surface functionalization was developed using polyethylene glycol. Morphology, and size distribution of AuNPs were evaluated by Field Emission Scanning Electron Microscopy. The average size of AuNSs, and AuNRs was 7.85nm and 7.96 x 31.47nm respectively. Fourier transform infrared spectrometry was performed to corroborate the presence of PEG in the AuNPs surface. Additionally, suspensions of AuNSs and AuNRs were evaluated by UV-Vis spectroscopy. Gold nanoparticles were stored for several days at room temperature and it was observed that the colloidal stability increased once gold nanoparticles were coated with PEG due to the shield formed in the surface of the NPs and the increase in size which were 9.65±1.90 nm of diameter for AuNSs and for AuNRs were 29.03±5.88 and 8.39±1.02 nm for length and transverse axis, respectively.

scholarly journals Photochemical Synthesis of Gold and Silver Nanoparticles—A Review

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4585
Author(s):  
Nicole Jara ◽  
Nataly S. Milán ◽  
Ashiqur Rahman ◽  
Lynda Mouheb ◽  
Daria C. Boffito ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Chemistry ◽  
Metallic Nanoparticles ◽  
Colloidal Stability ◽  
Photochemical Synthesis ◽  
Gold And Silver Nanoparticles ◽  
Future Developments ◽  
Technological Advances ◽  
Experimental Parameters ◽  
Sophisticated Equipment

Nanomaterials have supported important technological advances due to their unique properties and their applicability in various fields, such as biomedicine, catalysis, environment, energy, and electronics. This has triggered a tremendous increase in their demand. In turn, materials scientists have sought facile methods to produce nanomaterials of desired features, i.e., morphology, composition, colloidal stability, and surface chemistry, as these determine the targeted application. The advent of photoprocesses has enabled the easy, fast, scalable, and cost- and energy-effective production of metallic nanoparticles of controlled properties without the use of harmful reagents or sophisticated equipment. Herein, we overview the synthesis of gold and silver nanoparticles via photochemical routes. We extensively discuss the effect of varying the experimental parameters, such as the pH, exposure time, and source of irradiation, the use or not of reductants and surfactants, reagents’ nature and concentration, on the outcomes of these noble nanoparticles, namely, their size, shape, and colloidal stability. The hypothetical mechanisms that govern these green processes are discussed whenever available. Finally, we mention their applications and insights for future developments.

A highly efficient ligand exchange reaction on gold nanoparticles: preserving their size, shape and colloidal stability

RSC Advances ◽  
2014 ◽  
Vol 4 (64) ◽  
pp. 34217-34220 ◽  
Author(s):  
Melissa R. Dewi ◽  
Geoffry Laufersky ◽  
Thomas Nann
Keyword(s):  
Gold Nanoparticles ◽  
Solvent Extraction ◽  
Exchange Reaction ◽  
Ligand Exchange ◽  
Colloidal Stability ◽  
Ligand Exchange Reaction ◽  
Highly Efficient

Almost complete ligand exchange and excellent colloidal stability of gold nanoparticles can be achieved by a new solvent extraction based method.

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