scholarly journals A Textile-Based Microfluidic Platform for the Detection of Cytostatic Drug Concentration in Sweat Samples

2020 ◽  
Vol 10 (12) ◽  
pp. 4392 ◽  
Author(s):  
Goran M. Stojanović ◽  
Maja M. Radetić ◽  
Zoran V. Šaponjić ◽  
Marija B. Radoičić ◽  
Milan R. Radovanović ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Cost Effective ◽  
Electrical Contacts ◽  
Cytostatic Drug ◽  
Variable Resistor ◽  
Microfluidic Platform ◽  
Textile Fabrics ◽  
Drug Concentrations ◽  
Artificial Sweat ◽  
Conductive Textile

This work presents a new multilayered microfluidic platform, manufactured using a rapid and cost-effective xurography technique, for the detection of drug concentrations in sweat. Textile fabrics made of cotton and polyester were used as a component of the platform, and they were positioned in the middle of the microfluidic device. In order to obtain a highly conductive textile, the fabrics were in situ coated with different amounts of polyaniline and titanium dioxide nanocomposite. This portable microfluidic platform comprises at least three layers of optically transparent and flexible PVC foils which were stacked one on top of the other. Electrical contacts were provided from the edge of the textile material when a microfluidic variable resistor was actually created. The platform was tested in plain artificial sweat and in artificial sweat with a dissolved cytostatic test drug, cyclophosphamide, of different concentrations. The proposed microfluidic device decreased in resistance when the sweat was applied. In addition, it could successfully detect different concentrations of cytostatic medication in the sweat, which could make it a very useful tool for simple, reliable, and fast diagnostics.

scholarly journals Microsphere-Based Microfluidic Device for Plasma Separation and Potential Biochemistry Analysis Applications

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 487
Author(s):  
Hongyan Xu ◽  
Zhangying Wu ◽  
Jinan Deng ◽  
Jun Qiu ◽  
Ning Hu ◽  
...  
Keyword(s):  
Blood Sample ◽  
Microfluidic Device ◽  
Biochemical Analysis ◽  
Cost Effective ◽  
Clinical Diagnostics ◽  
Correction Factors ◽  
Separation Device ◽  
Blood Samples ◽  
Plasma Separation ◽  
Blood Biochemical

The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.

scholarly journals Improving the Role and Contribution of Pharmacokinetic Analyses in Antimalarial Drug Clinical Trials

2014 ◽  
Vol 58 (10) ◽  
pp. 5643-5649 ◽  
Author(s):  
Katherine Kay ◽  
Eva Maria Hodel ◽  
Ian M. Hastings
Keyword(s):  
Antimalarial Drug ◽  
Theoretical Data ◽  
Drug Efficacy ◽  
Cost Effective ◽  
Field Studies ◽  
World Health ◽  
Efficacy Trials ◽  
Drug Concentrations ◽  
Health Organization ◽  
Study Designs

ABSTRACTIt is now World Health Organization (WHO) policy that drug concentrations on day 7 be measured as part of routine assessment in antimalarial drug efficacy trials. The rationale is that this single pharmacological measure serves as a simple and practical predictor of treatment outcome for antimalarial drugs with long half-lives. Herein we review theoretical data and field studies and conclude that the day 7 drug concentration (d7c) actually appears to be a poor predictor of therapeutic outcome. This poor predictive capability combined with the fact that many routine antimalarial trials will have few or no failures means that there appears to be little justification for this WHO recommendation. Pharmacological studies have a huge potential to improve antimalarial dosing, and we propose study designs that use more-focused, sophisticated, and cost-effective ways of generating these data than the mass collection of single d7c concentrations.

scholarly journals Microfluidic on-demand droplet generation, storage, retrieval, and merging for single-cell pairing

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Hesam Babahosseini ◽  
Tom Misteli ◽  
Don L. DeVoe
Keyword(s):  
Single Cell ◽  
Aqueous Phase ◽  
Microfluidic Device ◽  
Droplet Generation ◽  
Microfluidic Platform ◽  
On Demand

A multifunctional microfluidic platform combining on-demand aqueous-phase droplet generation, multi-droplet storage, and controlled merging of droplets selected from a storage library in a single integrated microfluidic device is described.

scholarly journals Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays

2007 ◽  
Vol 53 (7) ◽  
pp. 1323-1329 ◽  
Author(s):  
Dianping Tang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Tumor Markers ◽  
Microfluidic Device ◽  
Simultaneous Detection ◽  
Reference Electrode ◽  
Cost Effective ◽  
Sio2 Nanoparticles ◽  
Magnetic Bead ◽  
Label Free ◽  
Specific Tumor ◽  
Protein Diagnostics

Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was <0.5 μg/L (or <0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were <4.5% and 8.7% for analyte concentrations >5 μg/L (or >5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was <10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

A microfluidic device for the batch adsorption of a protein on adsorbent particles

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3656-3665 ◽  
Author(s):  
Hoon Suk Rho ◽  
Alexander Thomas Hanke ◽  
Marcel Ottens ◽  
Han Gardeniers
Keyword(s):  
Adsorption Isotherm ◽  
Microfluidic Device ◽  
Batch Adsorption ◽  
Single Experiment ◽  
Microfluidic Platform

A microfluidic platform or “microfluidic batch adsorption device” is presented, which performs two sets of 9 parallel protein incubations with/without adsorbent particles to achieve an adsorption isotherm of a protein in a single experiment.

scholarly journals A microfluidic platform for cultivating ovarian cancer spheroids and testing their responses to chemotherapies

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Neda Dadgar ◽  
Alan M. Gonzalez-Suarez ◽  
Pouria Fattahi ◽  
Xiaonan Hou ◽  
John S. Weroha ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Drug Testing ◽  
Microfluidic Devices ◽  
Needle Aspiration ◽  
High Concentration ◽  
Microfluidic Platform ◽  
Drug Concentrations

Abstract There is increasing interest in utilizing in vitro cultures as patient avatars to develop personalized treatment for cancer. Typical cultures utilize Matrigel-coated plates and media to promote the proliferation of cancer cells as spheroids or tumor explants. However, standard culture conditions operate in large volumes and require a high concentration of cancer cells to initiate this process. Other limitations include variability in the ability to successfully establish a stable line and inconsistency in the dimensions of these microcancers for in vivo drug response measurements. This paper explored the utility of microfluidics in the cultivation of cancer cell spheroids. Six patient-derived xenograft (PDX) tumors of high-grade serous ovarian cancer were used as the source material to demonstrate that viability and epithelial marker expression in the microfluidic cultures was superior to that of Matrigel or large volume 3D cultures. To further demonstrate the potential for miniaturization and multiplexing, we fabricated multichamber microfluidic devices with integrated microvalves to enable serial seeding of several chambers followed by parallel testing of several drug concentrations. These valve-enabled microfluidic devices permitted the formation of spheroids and testing of seven drug concentrations with as few as 100,000 cancer cells per device. Overall, we demonstrate the feasibility of maintaining difficul-to-culture primary cancer cells and testing drugs in a microfluidic device. This microfluidic platform may be ideal for drug testing and personalized therapy when tumor material is limited, such as following the acquisition of biopsy specimens obtained by fine-needle aspiration.

scholarly journals Ultrasensitive multi-species detection of CRISPR-Cas9 by a portable centrifugal microfluidic platform

2019 ◽  
Vol 11 (5) ◽  
pp. 559-565 ◽  
Author(s):  
Christopher R. Phaneuf ◽  
Kyle J. Seamon ◽  
Tyler P. Eckles ◽  
Anchal Sinha ◽  
Joseph S. Schoeniger ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
Clinical Sample ◽  
Microfluidic Platform ◽  
Species Detection

Combined activity- and immunoassays for CRISPR/Cas9 on a portable microfluidic device with integrated sample preparation from clinical sample matrices.

scholarly journals Citizen-Led Sampling to Monitor Phosphate Levels in Freshwater Environments Using a Simple Paper Microfluidic Device

2021 ◽  
Author(s):  
Samantha Richardson ◽  
Alexander Iles ◽  
Jeanette M. Rotchell ◽  
Tim Charlson ◽  
Annabel Hanson ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Mobile Technology ◽  
High Frequency ◽  
Microfluidic Devices ◽  
Cost Effective ◽  
Smartphone App ◽  
Test Device ◽  
Monitoring Campaign ◽  
Paper Microfluidic ◽  
Freshwater Environments

We demonstrate how a combination of paper microfluidic devices and handheld mobile technology can be used by citizen scientists to carry out a sustained water monitoring campaign. We have developed a paper-based analysis device and a 3 minute sampling workflow that requires no more than a container, a test device and a smartphone app. The contaminant measured in these pilots are phosphates, detectable down to 3 mg L<sup>-1</sup>. Together these allow volunteers to successfully carry out cost-effective, high frequency, phosphate monitoring over an extended geographies and periods.

scholarly journals E-textiles prototypes and applications on wearable devices

2021 ◽  
Vol 7 (5) ◽  
pp. 169-171
Author(s):  
Renato Giacomini ◽  
Jéssica Maia Piccirillo
Keyword(s):  
Electronic Devices ◽  
Scientific Research ◽  
Wearable Devices ◽  
Proof Of Concept ◽  
Smart Textiles ◽  
Textile Fabrics ◽  
Conductive Textile

This scientific research has the purpose of studying conductive textiles, also known as "smart" textiles. At the ending of the study, we performed the assembly of functional prototypes to give the technology proof of concept to further studies. Between these prototypes are the "smart" jacket, the "smart" pillow, and the "smart" t-shirt. All of these prototypes have electronic devices integrated with conductive textile fabrics and yarns. The functionality of the prototypes involves the obtained vital signals from the user's body, such as heartbeat, and identification of the "touch" made by the user's hand to send commands to a computer or a cellphone screen.

scholarly journals A droplet microfluidic platform for efficient enzymatic chromatin digestion enables robust determination of nucleosome positioning

Lab on a Chip ◽  
2018 ◽  
Vol 18 (17) ◽  
pp. 2583-2592 ◽  
Author(s):  
Yi Xu ◽  
Jeong-Heon Lee ◽  
Zhaoyu Li ◽  
Liguo Wang ◽  
Tamas Ordog ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nucleosome Positioning ◽  
Microfluidic Platform

A microfluidic device was designed to efficiently fragment chromatin for downstream epigenetic assays.

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