scholarly journals Ultrasensitive multi-species detection of CRISPR-Cas9 by a portable centrifugal microfluidic platform

2019 ◽  
Vol 11 (5) ◽  
pp. 559-565 ◽  
Author(s):  
Christopher R. Phaneuf ◽  
Kyle J. Seamon ◽  
Tyler P. Eckles ◽  
Anchal Sinha ◽  
Joseph S. Schoeniger ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
Clinical Sample ◽  
Microfluidic Platform ◽  
Species Detection

Combined activity- and immunoassays for CRISPR/Cas9 on a portable microfluidic device with integrated sample preparation from clinical sample matrices.

scholarly journals Microfluidic on-demand droplet generation, storage, retrieval, and merging for single-cell pairing

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Hesam Babahosseini ◽  
Tom Misteli ◽  
Don L. DeVoe
Keyword(s):  
Single Cell ◽  
Aqueous Phase ◽  
Microfluidic Device ◽  
Droplet Generation ◽  
Microfluidic Platform ◽  
On Demand

A multifunctional microfluidic platform combining on-demand aqueous-phase droplet generation, multi-droplet storage, and controlled merging of droplets selected from a storage library in a single integrated microfluidic device is described.

A microfluidic device for the batch adsorption of a protein on adsorbent particles

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3656-3665 ◽  
Author(s):  
Hoon Suk Rho ◽  
Alexander Thomas Hanke ◽  
Marcel Ottens ◽  
Han Gardeniers
Keyword(s):  
Adsorption Isotherm ◽  
Microfluidic Device ◽  
Batch Adsorption ◽  
Single Experiment ◽  
Microfluidic Platform

A microfluidic platform or “microfluidic batch adsorption device” is presented, which performs two sets of 9 parallel protein incubations with/without adsorbent particles to achieve an adsorption isotherm of a protein in a single experiment.

On-chip sample preparation for complete blood count from raw blood

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1533-1544 ◽  
Author(s):  
John Nguyen ◽  
Yuan Wei ◽  
Yi Zheng ◽  
Chen Wang ◽  
Yu Sun
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
Whole Blood ◽  
Blood Count ◽  
Complete Blood Count ◽  
Chip Sample ◽  
On Chip

We present a monolithic microfluidic device capable of on-chip sample preparation for both RBC and WBC measurements from whole blood.

scholarly journals A Textile-Based Microfluidic Platform for the Detection of Cytostatic Drug Concentration in Sweat Samples

2020 ◽  
Vol 10 (12) ◽  
pp. 4392 ◽  
Author(s):  
Goran M. Stojanović ◽  
Maja M. Radetić ◽  
Zoran V. Šaponjić ◽  
Marija B. Radoičić ◽  
Milan R. Radovanović ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Cost Effective ◽  
Electrical Contacts ◽  
Cytostatic Drug ◽  
Variable Resistor ◽  
Microfluidic Platform ◽  
Textile Fabrics ◽  
Drug Concentrations ◽  
Artificial Sweat ◽  
Conductive Textile

This work presents a new multilayered microfluidic platform, manufactured using a rapid and cost-effective xurography technique, for the detection of drug concentrations in sweat. Textile fabrics made of cotton and polyester were used as a component of the platform, and they were positioned in the middle of the microfluidic device. In order to obtain a highly conductive textile, the fabrics were in situ coated with different amounts of polyaniline and titanium dioxide nanocomposite. This portable microfluidic platform comprises at least three layers of optically transparent and flexible PVC foils which were stacked one on top of the other. Electrical contacts were provided from the edge of the textile material when a microfluidic variable resistor was actually created. The platform was tested in plain artificial sweat and in artificial sweat with a dissolved cytostatic test drug, cyclophosphamide, of different concentrations. The proposed microfluidic device decreased in resistance when the sweat was applied. In addition, it could successfully detect different concentrations of cytostatic medication in the sweat, which could make it a very useful tool for simple, reliable, and fast diagnostics.

scholarly journals A droplet microfluidic platform for efficient enzymatic chromatin digestion enables robust determination of nucleosome positioning

Lab on a Chip ◽  
2018 ◽  
Vol 18 (17) ◽  
pp. 2583-2592 ◽  
Author(s):  
Yi Xu ◽  
Jeong-Heon Lee ◽  
Zhaoyu Li ◽  
Liguo Wang ◽  
Tamas Ordog ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nucleosome Positioning ◽  
Microfluidic Platform

A microfluidic device was designed to efficiently fragment chromatin for downstream epigenetic assays.

scholarly journals Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Anja Gulliksen ◽  
Helen Keegan ◽  
Cara Martin ◽  
John O'Leary ◽  
Lars A. Solli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Clinical Sample ◽  
Point Of Care ◽  
Fluorescent Detection ◽  
Clinical Samples ◽  
Microfluidic Channels ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Hpv E6

The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.

scholarly journals Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Microfluidic Device ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Resolution Imaging

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.

A hydrogel sensor-based microfluidic platform for the quantitative and multiplexed detection of fertility markers for point-of-care immunoassays

2019 ◽  
Vol 11 (12) ◽  
pp. 1639-1650 ◽  
Author(s):  
Satish Kalme ◽  
Srinivasan Kandaswamy ◽  
Anusha Chandrasekharmath ◽  
Reeta Katiyar ◽  
Gokul Prasath Rajamanickam ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Microfluidic Platform ◽  
Multiplexed Detection ◽  
Hydrogel Particle ◽  
Multiplexed Immunoassay ◽  
Porous Hydrogel

We report a new point-of-care, multiplexed immunoassay platform based on 3D porous hydrogel particle sensors embedded into a plastic microfluidic device.

scholarly journals Automated sample preparation in a microfluidic culture device for cellular metabolomics

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3858-3865 ◽  
Author(s):  
Laura A. Filla ◽  
Katherine L. Sanders ◽  
Robert T. Filla ◽  
James L. Edwards
Keyword(s):  
Cell Culture ◽  
Sample Preparation ◽  
Microfluidic Device ◽  
Energy Metabolites ◽  
Automated Sample Preparation ◽  
Cellular Metabolomics

The microfluidic device for integrated cell culture and sample preparation reported here resulted in a ∼10-fold improvement over conventional lysis methods and enabled the detection of energy metabolites.

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