scholarly journals Changes in Plasma Fatty Acids, Free Amino Acids, Antioxidant Defense, and Physiological Stress by Oleuropein Supplementation in Pigs Prior to Slaughter

Antioxidants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 56 ◽  
Author(s):  
Ana I. Rey ◽  
Almudena de-Cara ◽  
Luis Calvo ◽  
Patricia Puig ◽  
Teresa Hechavarría
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Free Fatty Acids ◽  
Physiological Stress ◽  
Olive Tree ◽  
Plasma Metabolites ◽  
Total N ◽  
Antioxidant Power ◽  
Positive Effects ◽  
The Impact

Olive tree leaves are characterized for having not only a potent antioxidant power but also effects on glucose and lipid metabolism. The impact of the individual oleuropein (OLE), vitamin E + Se (VE), or a combined supplementation of oleuropein, vitamin E, and selenium (VEOLE) was evaluated on pig plasma metabolites under fasting prior to slaughter. VEOLE and OLE had lesser n-3 plasma polyunsaturated fatty acids and greater monounsaturated free fatty acids compared to control. The n-3-fatty acid mobilization was directly correlated with greater cystine and inversely with oxidized glutathione/reduced glutathione (GSSH/GSH) levels. This faster use of n-3 fatty acids might act as an indicator of glutathione synthesis mediated by an increase of cystine in plasma. Different correlations and linear adjustments were observed between plasma antioxidant power and free cystine, free glycine, free glutamine, monounsaturated free fatty acids, and total n-3. The best response to stress was found in VEOLE. Cortisol reached the greatest positive correlation with plasma total n-3 fatty acids, which suggests a faster uptake of n-3 for biological functions such as stress control or energy supply in the brain. From a practical point of view, an enhanced oxidative status as well as control of physiological stress prior to slaughter by the combined antioxidants supplementation might have positive effects on pork quality.

scholarly journals Selected nutrients determining the quality of different cuts of organic and conventional pork

2021 ◽  
Author(s):  
Karolina M. Wójciak ◽  
Michał Halagarda ◽  
Sascha Rohn ◽  
Paulina Kęska ◽  
Agnieszka Latoch ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Meat Products ◽  
Pork Meat ◽  
Total N ◽  
Iron And Zinc ◽  
Calcium Magnesium ◽  
Calcium Iron ◽  
Organic Meat

AbstractOrganic meat products are gaining consumer interest worldwide. The aim of this study was to investigate the effect of organic and conventional meat origin on nutritional determinants of the following pork meat cuts: loin, ham, and shoulder. Nutritional value of meat was based on selected indicators such as proximate composition, the concentration of cholesterol, vitamin E content and minerals and trace elements such as calcium, magnesium, potassium, copper, iron, and zinc, and the composition of fatty acids. The results of this study demonstrated that higher contents of protein and selected mineral compounds, as well as lower vitamin E concentration and different fatty acids (i.e., C12:0, C17:0, C17:1 n-7, C18:3 n-6, C24:0 and total n-3 polyunsaturated fatty acids (PUFA)) distinguish organic pork meat cuts from the conventional counterparts. The organically meat parts, especially the shoulder, were identified as a better source of copper, calcium, iron and zinc, while organic ham and loin had more potassium. On the other hand, organic hams were shown to have lower content of vitamin E in comparison to their conventional equivalents present. Also, in organic shoulders showed a higher n-6/n-3 ratio compared to meat of conventional origin.

scholarly journals Free Fatty Acids Impair FGF21 Action in HepG2 Cells

2015 ◽  
Vol 37 (5) ◽  
pp. 1767-1778 ◽  
Author(s):  
Mohamed Asrih ◽  
Christophe Montessuit ◽  
Jacques Philippe ◽  
François R. Jornayvaz
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Lipid Metabolism ◽  
Free Fatty Acids ◽  
Hepg2 Cells ◽  
Lipid Lowering ◽  
Fibroblast Growth Factor 21 ◽  
Beneficial Effects ◽  
Positive Effects

Background/Aims: Fibroblast growth factor 21 (FGF21) is a key mediator of glucose and lipid metabolism. However, the beneficial effects of exogenous FGF21 administration are attenuated in obese animals and humans with elevated levels of circulating free fatty acids (FFA). Methods: We investigated in vitro how FFA impact FGF21 effects on hepatic lipid metabolism. Results: In the absence of FFA, FGF21 reduced lipogenesis and increased lipid oxidation in HepG2 cells. Inhibition of lipogenesis was associated with a down regulation of SREBP-1c, FAS and SCD1. The lipid-lowering effect was associated with AMPK and ACC phosphorylation, and up regulation of CPT-1α expression. Further, FGF21 treatment reduced TNFα gene expression, suggesting a beneficial action of FGF21 on inflammation. In contrast, the addition of FFA abolished the positive effects of FGF21 on lipid metabolism. Conclusion: In the absence of FFA, FGF21 improves lipid metabolism in HepG2 cells and reduces the inflammatory cytokine TNFα. However, under high levels of FFA, FGF21 action on lipid metabolism and TNFα gene expression is impaired. Therefore, FFA impair FGF21 action in HepG2 cells potentially through TNFα.

scholarly journals Application of Sebum Lipidomics to Biomarkers Discovery in Neurodegenerative Diseases

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 819
Author(s):  
Stefania Briganti ◽  
Mauro Truglio ◽  
Antonella Angiolillo ◽  
Salvatore Lombardo ◽  
Deborah Leccese ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Observational Study ◽  
Neurodegenerative Diseases ◽  
Free Fatty Acids ◽  
Healthy Subjects ◽  
Skin Surface ◽  
Biosynthetic Pathways ◽  
Simultaneous Component Analysis ◽  
Lipid Species

Lipidomics is strategic in the discovery of biomarkers of neurodegenerative diseases (NDDs). The skin surface lipidome bears the potential to provide biomarker candidates in the detection of pathological processes occurring in distal organs. We investigated the sebum composition to search diagnostic and, possibly, prognostic, biomarkers of Alzheimer’s disease (AD) and Parkinson’s disease (PD). The observational study included 64 subjects: 20 characterized as “probable AD with documented decline”, 20 as “clinically established PD”, and 24 healthy subjects (HS) of comparable age. The analysis of sebum by GCMS and TLC retrieved the amounts (µg) of 41 free fatty acids (FFAs), 7 fatty alcohols (FOHs), vitamin E, cholesterol, squalene, and total triglycerides (TGs) and wax esters (WEs). Distributions of sebum lipids in NDDs and healthy conditions were investigated with multivariate ANOVA-simultaneous component analysis (ASCA). The deranged sebum composition associated with the PD group showed incretion of most composing lipids compared to HS, whereas only two lipid species (vitamin E and FOH14:0) were discriminant of AD samples and presented lower levels than HS sebum. Thus, sebum lipid biosynthetic pathways are differently affected in PD and AD. The characteristic sebum bio-signatures detected support the value of sebum lipidomics in the biomarkers search in NDDs.

scholarly journals Omega-3 fatty acids and cardiovascular disease risk: do we understand the relationship?

2009 ◽  
pp. S19-S26 ◽  
Author(s):  
M Vrablík ◽  
M Prusíková ◽  
M Šnejdrlová ◽  
L Zlatohlávek
Keyword(s):  
Cardiovascular Disease ◽  
Fatty Acids ◽  
Disease Risk ◽  
Large Body ◽  
Current Evidence ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Cvd Risk ◽  
Positive Effects ◽  
The Impact

There is a large body of evidence documenting the effects of long-chain polyunsaturated fatty acids with the first double bond at the third position from methyl-terminal (so called omega-3 fatty acids (FAs)) on different components of cardiovascular disease (CVD) risk. However, it may seem the more answers on the topic we learn, the more questions remain to be elucidated. There are three levels of evidence documenting the impact of fish omega-3 FAs on CVD risk. Epidemiological data have shown unequivocally the increased intake of fish is associated with lower CVD morbidity and mortality. Numerous experimental studies have shown (almost always) positive effects of omega-3 FAs on lipoprotein metabolism, coagulation and platelet function, endothelial function, arterial stiffness etc. Most importantly, there are a few prospective clinical endpoint trials (DART, JELIS, GISSI Prevenzione and GISSI-HF) that have examined the impact of omega-3 FAs supplementation on cardiovascular outcomes in different patient populations. Recent meta-analyses of these and other clinical studies have yielded somewhat conflicting results. In this review we will summarize current evidence of omega-3 FAs effects on cardiovascular risk focusing on new data from recent clinical trials as well as possible practical implications for clinical practice.

scholarly journals MARGRA Lamb Eating Quality and Human Health-Promoting Omega-3 Long-Chain Polyunsaturated Fatty Acid Profiles of Tattykeel Australian White Sheep: Linebreeding and Gender Effects

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1118
Author(s):  
Shedrach Benjamin Pewan ◽  
John Roger Otto ◽  
Robert Tumwesigye Kinobe ◽  
Oyelola Abdulwasiu Adegboye ◽  
Aduli Enoch Othniel Malau-Aduli
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Antioxidant Status ◽  
Gender Effects ◽  
Eating Quality ◽  
Omega 3 ◽  
Antioxidant Power ◽  
And Gender ◽  
The Impact ◽  
Long Chain

Health-conscious consumers increasingly demand healthier, tastier, and more nutritious meat, hence the continuous need to meet market specifications and demand for high-quality lamb. We evaluated the longissimus dorsi muscle of 147 Tattykeel Australian White (TAW) sheep fed on antioxidant-rich ryegrass pastures exclusive to MAGRA lamb brand for meat eating quality parameters of intramuscular fat (IMF) content, fat melting point (FMP) and omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). The aim was to assess the impact of linebreeding and gender on pasture-fed lamb eating quality and to test the hypothesis that variation in healthy lamb eating quality is a function of lamb gender and not its antioxidant status or inbreeding coefficient (IC). After solid-phase extraction and purification, phenolics and antioxidant enzyme activities were analysed by high-performance liquid chromatography and mass spectrometry. IMF and fatty acid composition were determined using solvent extraction and gas chromatography, respectively. IC was classified into low (0–5%), medium (6–10%) and high (>10%) and ranged from 0–15.6%. FMP and IMF ranged from 28 to 39 °C and 3.4% to 8.2%, with overall means of 34.6 ± 2.3 °C and 4.4 ± 0.2%, respectively, and n-3 LC-PUFA ranged from “source” to “good source” levels of 33–69 mg/100 g. Ewes had significantly (P ˂ 0.0001) higher IMF, C22:5n-3 (DPA), C22:6n-3 (DHA), C18:3n-6, C20:3, C22:4n-6, C22:5n-6, total monounsaturated (MUFA), PUFA and Σn-3 fatty acids and lower total saturated fatty acids (SFA) and FMP, than rams. As IC increased, there were no differences in FMP and IMF. Folin–Ciocalteu total phenolics, ferric reducing antioxidant power and antioxidant activities of glutathione peroxidase, catalase and superoxide dismutase enzymes did not differ by either gender or IC. This study provides evidence that IC is inconsequential in affecting antioxidant status, IMF, FMP and n-3 LC-PUFA in linebred and pasture-fed TAW sheep because the observed variation in individual fatty acids was mainly driven by gender differences between ewes and rams, hence the need to accept the tested hypothesis. This finding reinforces the consistent healthy eating quality of MARGRA lamb brand from TAW sheep regardless of its linebred origin.

Potentiation of the damaging action of free fatty acids on brain synaptosomes by vitamin E deficiency

1986 ◽  
Vol 102 (5) ◽  
pp. 1520-1522
Author(s):  
V. I. Skrypin ◽  
V. I. Brusovanik ◽  
L. M. Dzhaparidze ◽  
A. N. Erin ◽  
A. A. Selishcheva ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Free Fatty Acids ◽  
Vitamin E Deficiency ◽  
Brain Synaptosomes

scholarly journals A Comparative Study on Two Territorial Fishes: The Influence of Physical Enrichment on Aggressive Behavior

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1868
Author(s):  
Zonghang Zhang ◽  
Yiqiu Fu ◽  
Zhen Zhang ◽  
Xiumei Zhang ◽  
Shengcan Chen
Keyword(s):  
Aggressive Behavior ◽  
Environmental Enrichment ◽  
Physiological Stress ◽  
Fish Welfare ◽  
Black Rockfish ◽  
Positive Effects ◽  
High Enrichment ◽  
High Level ◽  
The Impact ◽  
Fat Greenling

Intraspecific aggression is detrimental to body/fin damage, physiological stress, and other problems in aquaculture. Environmental enrichment has been proposed to have positive effects on fish aggressive behavior, physiological stress, and fish welfare, but there are mixed results. Here, we examine the impact of physical enrichment levels (i.e., the intensity of physical enrichment) on aggression in black rockfish (Sebastes schlegelii) and fat greenling (Hexagrammos otakii). Generally, with the increase in the enrichment level, the frequency of the aggressive behavior of black rockfish gradually decreased. In contrast, a non-monotonous effect of the enrichment level on aggression was observed for fat greenling, with low and intermediate levels leading to no or more aggression, while a high enrichment level reduced aggression. After three days, the high-level enrichment groups in both rockfish and greenling reached social stability (i.e., a relatively stable social structure indicated by lower aggression), while aggression in the other groups continued increased. These results show the significant regulatory effect of enrichment levels on the aggressive behavior in both black rockfish and fat greenling. This study may promote the development of environmental enrichment measures, and it provides useful information for reducing fish aggression and improving fish welfare in aquaculture.

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