scholarly journals Investigation of In Vitro Antioxidant and Antibacterial Potential of Silver Nanoparticles Obtained by Biosynthesis Using Beech Bark Extract

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 459 ◽  
Author(s):  
Tanase ◽  
Berta ◽  
Coman ◽  
Roșca ◽  
Man ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Metal Ion ◽  
Color Change ◽  
Vital Role ◽  
Bark Extract ◽  
Ft Ir ◽  
Antioxidant And Antimicrobial Activity ◽  
Average Size

Green synthesis is one of the rapid and best ways for silver nanoparticles (AgNP) synthesis. In the present study, synthesis and bioactivity of AgNPs has been demonstrated using water beech (Fagus sylvatica L.) bark extract. The physical and chemical factors such as time, metal ion solution, and pH, which play a vital role in the AgNPs synthesis, were assessed. The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrometry, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Antioxidant and antimicrobial activity of the obtained AgNPs was evaluated. AgNPs were characterized by color change pattern, and the broad peak obtained at 420–475 nm with UV-Vis confirmed the synthesis of AgNPs. FT-IR results confirmed that phenols and proteins of beech bark extract are mainly responsible for capping and stabilization of synthesized AgNPs. TEM micrographs showed spherical or rarely polygonal and triangular particles with an average size of 32 nm at pH = 9, and 62 nm at pH = 4. Furthermore, synthesized AgNPs were found to exhibit antioxidant activity and have antibacterial effect against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. These results indicate that bark extract of F. sylvatica L. is suitable for synthesizing stable AgNPs, which act as an excellent antimicrobial agent.

Green Synthesis of Silver Nanoparticles via Phormidium sp. nov. (Cyanophyceae): Amelioration, Characterization and Assessment of the Antibacterial Potential Against Methicillin Resistant Staphylococcus aureus

2021 ◽  
Vol 13 (2) ◽  
pp. 209-216
Author(s):  
Nermin A. El-Semary ◽  
Mona Mabrouk ◽  
Ahmed H. Faraag ◽  
Mona Kilany ◽  
Shymaa H. Omran ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Color Change ◽  
Silver Ions ◽  
Time Saving ◽  
Methicillin Resistant ◽  
Ft Ir ◽  
Ir Analysis ◽  
Stability Results ◽  
Ft Ir Spectroscopy

The use and manufacturing of silver nanoparticles (AgNPs) have recently received considerable attention in many fields. Methicillin resistant Staphylococcus aureus (MRSA) causes infectious diseases in humans. Therefore, the aim of this work was to bio-synthesize AgNPs using the cyanobacteria Phormidium sp. extract and to use this extract with AgNPs to kill MRSA. Extracellular bio-formation of AgNPs by Phormidium sp. was scrutinized at various pH points and temperatures. Then, characterization and assessment of the antimicrobial power of the produced particles were performed. Extract and extract + AgNPs were screened using Fourier Transform Infra-Red (FT-IR) spectroscopy and electron microscopy. The manufacturing and fastness of AgNPs were monitored visually (by color change) and spectroscopically. FT-IR analysis uncovered the biomolecules in the extract that caused the reduction of silver ions, protected them from aggregation and supplied them with high stability. Results indicated that AgNPs are spheres of 9 nm diameter. AgNPs combined with the extract proved to be a powerful antibacterial factor against MRSA. Consequently, biosynthesis of silver nano-spheres using Phormidium sp. with high antagonistic potency against MRSA is considered as an eco-friendly, time-saving strategy and with potential medicinal significance.

scholarly journals Antibacterial and Antioxidant Potential of Silver Nanoparticles Biosynthesized Using the Spruce Bark Extract

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1541 ◽  
Author(s):  
Corneliu Tanase ◽  
Lavinia Berta ◽  
Năstaca Alina Coman ◽  
Ioana Roșca ◽  
Adrian Man ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Metal Ion ◽  
Color Change ◽  
Biological Activities ◽  
Stem Bark ◽  
Bark Extract ◽  
Solution Ph ◽  
Spruce Bark ◽  
Vis Spectroscopy

Biosynthesized silver nanoparticles (AgNPs) are widely used in Pharmacy and Medicine. In particular, AgNPs synthesized and mediated by plant extracts have shown topossess several biological activities. In the present study, AgNPs were synthesized using Picea abies L. stem bark extract as reducing agent. Factors, such as metal ion solution, pH, and time, which play a role in the AgNPs synthesis, were assessed. The synthesized AgNPs were characterized by Ultraviolet-Visible Spectrometry, Fourier transform infrared spectroscopy, and Transmission Electron Microscopy (TEM). Further, the study has been extended to evaluate the antimicrobial and antioxidant activity of AgNPs. The broad peak obtained at 411–475 nm (UV-Vis spectroscopy), and the color change pattern, confirmed the synthesis of AgNPs. TEM results showed spherical or rarely polygonal AgNPs with an average size of 44 nm at pH = 9. The AgNPs showed antioxidant activity and antibacterial effect against human pathogenic Gram-positive and Gram-negative bacteria. The results show that spruce bark extract is suitable for obtaining AgNPs, with antibacterial and antioxidant activity.

scholarly journals In vitro and in vivo evaluation of antibacterial activity of Bridelia ferruginea extracts on some clinical isolates

2018 ◽  
Vol 7 (4) ◽  
pp. 392-398
Author(s):  
B.T Yunana ◽  
◽  
B. B Bukar ◽  
J. C Aguiyi ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Root Bark ◽  
Zone Of Inhibition ◽  
Bridelia Ferruginea

The ethanol extracts of root, bark and leaf of Bridelia ferruginea was investigated for antibacterial activity against clinical isolate of Staphylococcus aureus and Escherichia coli. The extracts had significant antibacterial activity in vitro at concentration of 25 mg/ml, 50 mg/ml, 100 mg/ml and 200 mg/ml and in vivo at dose of 50 mg/kg and 100 mg/kg. The root extract in vitro had the highest zone of inhibition, followed by the bark extract for both Staphylococcus aureus and Escherichia coli. The concentration of 200 mg/ml had the highest zone of inhibition in vitro. The minimum inhibitory concentration (MIC) showed a decreasing inhibitory effect of the plant extracts for both Staphylococcus aureus and Escherichia coli as the concentration decreases with root having 3.125 mg/ml, bark having 6.25 mg/ml and leaf having 25 mg/ml for Staphylococcus aureus and Escherichia coli. Likewise, the minimum bactericidal concentration (MBC) showed decreasing bactericide effects with decrease concentration with root having 12.5 mg/ml, bark having 12.5 mg/ml and leaf having 25 mg/ml for Escherichia coli while root had 6.25mg/ml, bark had 12.5mg/ml and leaf had 25mg/ml for Staphylococcus aureus. The in vivo investigation showed that the root and bark extract exhibited antibacterial activity on both Staphylococcus aureus and Escherichia coli at doses of 100mg/kg and 50mg/kg; the root extract had higher activity than the bark and root/bark combined. The dose of 100 mg/kg had the highest colonies reduction for Staphylococcus aureus and Escherichia coli in vivo. Preliminary phytochemical screening of root, bark and leaves of Bridelia ferruginea revealed the presence of tannins, flavonoids, carbohydrates, cardiac glycoside (root, bark and leaves), saponins (root and bark). The presence of tannins, saponins, flavonoid, cardiac glycoside and carbohydrate in the bark and root extracts of the plant indicates that the bark and root extracts were pharmacological importance

A New Frontier Drug Development in Nanomedicine and Itsanti-Urolithiatic Activity of Kalanchoe Pinnata

2021 ◽  
Vol 37 (2) ◽  
pp. 444-449
Author(s):  
F. Janeeta Priya ◽  
A. Leema Rose ◽  
S. Vidhya ◽  
A Arputharaj ◽  
S. Akshana ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Urinary Calculi ◽  
X Ray Diffraction ◽  
Tem Analysis ◽  
New Frontier ◽  
Kalanchoe Pinnata ◽  
Stock Pile ◽  
Ft Ir

Latterly, the green synthesis of metallic nanoparticles has stock pile and terminal interest over the last decade due to their idiosyncratic properties that make them felicitous in various fields of science and technology. In this study the silver nanoparticles can be manufacture by using the leaf extract of Kalanchoe pinnata plant using microwave radiation method. The nanoparticles that are synthesized by plants are non-toxic and eco-friendly and it is characterized by using UV-Visible, FT-IR , X-ray diffraction (XRD) and TEM.The crystalline natures of the synthesized silver nanoparticles were identified by XRD and its shape was confirmed by TEM analysis. Anti-urolithiatic activity of synthesized silver nanoparticles was accomplished which reduces the growth of kidney stone which was appraisal by using the In-Vitro growth of urinary calculi.

Development, Characterization and Anticancer Evaluation of Silver Nanoparticles from Dalbergia sissoo Leaf Extracts

2020 ◽  
Vol 16 (2) ◽  
pp. 145-151
Author(s):  
Aakash Deep ◽  
Mitali Verma ◽  
Rakesh K. Marwaha ◽  
Arun K. Sharma ◽  
Beena Kumari
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Activity ◽  
Metal Ion ◽  
Color Change ◽  
Fixed Ratio ◽  
Cancer Cell Line ◽  
Dalbergia Sissoo ◽  
Leaf Extracts ◽  
Standard Drug

Aim: The objective behind this present work is the development and characterization of silver nanoparticles from Dalbergia sissoo leaf extracts and the analysis of anticancer activity. Methods: Silver nanoparticles were prepared by using the aqueous solution of Dalbergia sisoo leaf extract and silver nitrate. The formation of nanoparticles was determined by the color change during the preparation of plant extract to metal ion in a fixed ratio. The prepared nanoparticles were then characterized by TEM, FTIR, DLS, XRD, and SEM. Silver nanoparticles were also evaluated for anticancer activity. Results: Synthesized silver nanoparticles were having good anticancer activity against MCF 7 cancer cell line as compared to the standard drug Doxorubicin. Conclusion: The particle size of nanoparticles was found to lie in the range of 10 to 50 nm.

scholarly journals Design and Characterization of Endostatin-Loaded Nanoparticles for In Vitro Antiangiogenesis in Squamous Cell Carcinoma

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Samson A. Adeyemi ◽  
Yahya E. Choonara ◽  
Pradeep Kumar ◽  
Lisa C. du Toit ◽  
Viness Pillay
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Viability ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Antiangiogenic Effect ◽  
Tumor Ph ◽  
Ft Ir ◽  
Average Size

The aim of this study is to effectively enhance antitumor activities of endostatin by preparing polymeric nanocarriers. NMR and FT-IR spectra confirmed the successful grafting of the CHT-g-PEI and CHT-g-PEI-PEG-NH2 conjugates. SEM micrographs confirmed the shape of endostatin-loaded nanoparticles to be spherical while both TEM and zeta size results showed nanoparticle’s average size to be 100.6 nm having a positively charged surface with zeta potential of 7.95 mV. The concentrations of CHT and TPP as well as the changing pH conditions account for the increased swelling pattern of endostatin-loaded nanoparticles and influenced endostatin release in vitro. PEI increased the overall amine protonation while PEG aggravated endostatin encapsulation and release. Nanoparticles swell and release endostatin at acidic tumor pH of 6.8 compared to physiological pH of 7.4. The native CHT-g-PEI-PEG-NH2 conjugate showed high cytocompatibility above 80% cell viability across tested formulations. Endostatin-loaded nanoparticles showed a significant reduction in cell viability across tested formulations, with 5.32% cell death at 125 μg/mL and 13.36% at 250 μg/mL following 24 hours’ incubation period. Interestingly, more than a fourfold (61.68%) increment in cytotoxicity was observed at nanoparticle concentration of 1000 μg/mL. It was concluded that CHT-g-PEI-PEG-NH2 is an effective cargo for endostatin delivery with antiangiogenic effect in squamous cell carcinoma.

scholarly journals The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study

2021 ◽  
Vol 55 (4) ◽  
pp. 285
Author(s):  
Bernadette Dian Novita ◽  
Silvia Sutandhio
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Water Extract ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Bark Extract ◽  
Native Plant ◽  
Cinnamomum Burmannii

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia

scholarly journals Bio-Inspired Fabrication of Silver Nanoparticles Using High Altitude Squamulose Lichen Extract and Evaluation of its Antioxidant, Anticandida and Cytotoxic properties

2021 ◽  
Author(s):  
S Shanthi ◽  
V. Uma Maheshwari Nallal ◽  
Krishnan Anand ◽  
Balasubramani Ravindran ◽  
Soon Woong Chang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
High Altitude ◽  
Usnic Acid ◽  
Nanoparticle Synthesis ◽  
Spectroscopic Studies ◽  
Binding Energies ◽  
Average Size ◽  
Nano Medicine

Abstract Bio-inspired nanoparticle synthesis has attracted substantial interest among the scientific society owing to its eco-friendly and non-toxic nature. In the present study, Silver nanoparticles (AgNPs) were synthesized using high altitude squamulose lichen – Cladonia subradiata and characterized using different techniques. The antioxidant and anticandida activity of AgNPs were evaluated using multiple in-vitro assays. In-silico molecular docking analysis and in-vitro cytotoxic assay was performed to determine the anti-cancer potential of synthesized AgNPs. The results of the spectroscopic studies revealed the successful synthesis of AgNPs and the presence of different functional groups suggesting the involvement of phytocompounds in the reduction and capping of AgNPs. The average size of the AgNPs was 20 nm and predominantly spherical in shape. AgNPs demonstrated excellent DPPH free radicals scavenging activity with an IC50 value of 7.51 ± 0.4 µg/mL. C.albicans was identified as the most susceptible strain from the anticandida studies. Usnic acid and Pulvinic acid exhibited low binding energies and showed excellent inhibition interaction with EGFR lung cancer protein. The in-vitro cytotoxic results were impressive with an IC50 value of 28.75 µg/mL for A549 lung cancer cells treated with AgNPs. Thus, the study demonstrates the effective and non-toxic synthesis of AgNPs using a less explored lichen extract as a promising anticandida and anticancer agent in the field of nano-medicine.

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