scholarly journals Antibacterial and Antioxidant Potential of Silver Nanoparticles Biosynthesized Using the Spruce Bark Extract

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1541 ◽  
Author(s):  
Corneliu Tanase ◽  
Lavinia Berta ◽  
Năstaca Alina Coman ◽  
Ioana Roșca ◽  
Adrian Man ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Metal Ion ◽  
Color Change ◽  
Biological Activities ◽  
Stem Bark ◽  
Bark Extract ◽  
Solution Ph ◽  
Spruce Bark ◽  
Vis Spectroscopy

Biosynthesized silver nanoparticles (AgNPs) are widely used in Pharmacy and Medicine. In particular, AgNPs synthesized and mediated by plant extracts have shown topossess several biological activities. In the present study, AgNPs were synthesized using Picea abies L. stem bark extract as reducing agent. Factors, such as metal ion solution, pH, and time, which play a role in the AgNPs synthesis, were assessed. The synthesized AgNPs were characterized by Ultraviolet-Visible Spectrometry, Fourier transform infrared spectroscopy, and Transmission Electron Microscopy (TEM). Further, the study has been extended to evaluate the antimicrobial and antioxidant activity of AgNPs. The broad peak obtained at 411–475 nm (UV-Vis spectroscopy), and the color change pattern, confirmed the synthesis of AgNPs. TEM results showed spherical or rarely polygonal AgNPs with an average size of 44 nm at pH = 9. The AgNPs showed antioxidant activity and antibacterial effect against human pathogenic Gram-positive and Gram-negative bacteria. The results show that spruce bark extract is suitable for obtaining AgNPs, with antibacterial and antioxidant activity.

scholarly journals Phyto-Functionalized Silver Nanoparticles Derived from Conifer Bark Extracts and Evaluation of Their Antimicrobial and Cytogenotoxic Effects

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 217
Author(s):  
Irina Macovei ◽  
Simon Vlad Luca ◽  
Krystyna Skalicka-Woźniak ◽  
Liviu Sacarescu ◽  
Petronela Pascariu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Picea Abies ◽  
Biological Activities ◽  
Pinus Nigra ◽  
Attenuated Total Reflection ◽  
Narrow Particle Size Distribution ◽  
Bark Extract ◽  
Resonance Band ◽  
Vis Spectroscopy

Silver nanoparticles synthesized using plant extracts as reducing and capping agents showed various biological activities. In the present study, colloidal silver nanoparticle solutions were produced from the aqueous extracts of Picea abies and Pinus nigra bark. The phenolic profile of bark extracts was analyzed by liquid chromatography coupled to mass spectrometry. The synthesis of silver nanoparticles was monitored using UV-Vis spectroscopy by measuring the Surface Plasmon Resonance band. Silver nanoparticles were characterized by attenuated total reflection Fourier transform infrared spectroscopy, Raman spectroscopy, dynamic light scattering, scanning electron microscopy, energy dispersive X-ray and transmission electron microscopy analyses. The antimicrobial and cytogenotoxic effects of silver nanoparticles were evaluated by disk diffusion and Allium cepa assays, respectively. Picea abies and Pinus nigra bark extract derived silver nanoparticles were spherical (mean hydrodynamic diameters of 78.48 and 77.66 nm, respectively) and well dispersed, having a narrow particle size distribution (polydispersity index values of 0.334 and 0.224, respectively) and good stability (zeta potential values of −10.8 and −14.6 mV, respectively). Silver nanoparticles showed stronger antibacterial, antifungal, and antimitotic effects than the bark extracts used for their synthesis. Silver nanoparticles obtained in the present study are promising candidates for the development of novel formulations with various therapeutic applications.

scholarly journals Phytochemicals, Antimicrobials and Antioxidants Studies of the Stem Bark Extract from Calophyllum ferrugineum

2020 ◽  
Vol 17 (2) ◽  
pp. 1
Author(s):  
Izzah Afifah Noh ◽  
Vivien Yi Mian Jong
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Biological Activities ◽  
Stem Bark ◽  
Extraction Process ◽  
Bark Extract ◽  
Chemical Components ◽  
Isolation And Purification ◽  
Antioxidant Agents ◽  
Ic50 Values

Traditionally, Calophyllum genus was used for swollen gums, arthritis, diarrhea, chronic abscess, skin infections, and lesions treatment. C. ferrugineum from Sarawak has least studied about their phytochemical constituents and biological activities. The objectives are to isolate and characterize the chemical components from C. ferrugineum and to determine their antimicrobial and antioxidant activity. The plant stem barks were collected from National Park in Sarawak and underwent extraction process. The extracts underwent isolation and purification processes by using several chromatographic methods. Structural elucidation was achieved by using infrared, MS and NMR spectra. The isolation process from the species has afforded 5 known compounds;  isocalanone (1) and 1-hydroxy-7-methoxy-9H-xanthen-9-one (2), lupeol (3), friedelin (4) and diethylene glycol dibenzoate (5). The chloroform and methanol extract showed strong inhibitions against S. aureus, B. subtilis, P. aeruginosa and E. coli with the MIC and MBC values ranging from 225 and 112.5 µg/mL. Isocalanone (1) and the methanol extract of C. ferrugineum showed potent antioxidant activity with the IC50 values of 28 ± 5.23 and 35 ± 3.69 µg/mL compared to ascorbic acid, respectively. The results obtained from this study emphasized the potential of the species as antimicrobial and antioxidant agents.

scholarly journals Investigation of In Vitro Antioxidant and Antibacterial Potential of Silver Nanoparticles Obtained by Biosynthesis Using Beech Bark Extract

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 459 ◽  
Author(s):  
Tanase ◽  
Berta ◽  
Coman ◽  
Roșca ◽  
Man ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Metal Ion ◽  
Color Change ◽  
Vital Role ◽  
Bark Extract ◽  
Ft Ir ◽  
Antioxidant And Antimicrobial Activity ◽  
Average Size

Green synthesis is one of the rapid and best ways for silver nanoparticles (AgNP) synthesis. In the present study, synthesis and bioactivity of AgNPs has been demonstrated using water beech (Fagus sylvatica L.) bark extract. The physical and chemical factors such as time, metal ion solution, and pH, which play a vital role in the AgNPs synthesis, were assessed. The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrometry, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Antioxidant and antimicrobial activity of the obtained AgNPs was evaluated. AgNPs were characterized by color change pattern, and the broad peak obtained at 420–475 nm with UV-Vis confirmed the synthesis of AgNPs. FT-IR results confirmed that phenols and proteins of beech bark extract are mainly responsible for capping and stabilization of synthesized AgNPs. TEM micrographs showed spherical or rarely polygonal and triangular particles with an average size of 32 nm at pH = 9, and 62 nm at pH = 4. Furthermore, synthesized AgNPs were found to exhibit antioxidant activity and have antibacterial effect against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. These results indicate that bark extract of F. sylvatica L. is suitable for synthesizing stable AgNPs, which act as an excellent antimicrobial agent.

scholarly journals Biological Activities and Characterization of Silver Nanoparticles from Prunus Prescia (L.) Batsch

2021 ◽  
Vol 10 (3) ◽  
pp. 2466-2482
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Leaf Extract ◽  
Prunus Persica ◽  
Color Change ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Face Centered Cubic ◽  
Plasmon Band ◽  
Vis Spectroscopy

In this paper, Prunus persica L. Batsch (wild and variety) plants have been used for the bioreduction of silver ions to silver nanoparticles. Aqueous leaf extract of plants was treated with silver nitrate. The mixture's color changed from pale yellow to dark brown Prunus persica (variety) and dark yellow to greyish brown Prunus persica (wild). The color change was the first indication of silver nanoparticle synthesis, further confirmed by UV-Vis spectroscopy. The surface Plasmon band exhibited absorption peaks for Prunus persica wild and variety leaf extract at 468 nm, 492 nm, 462 nm. The silver nanoparticles were further characterized using X-Ray Diffraction that carried out the crystallographic nature by exhibiting 2θ value from 10˚ to 90˚ corresponding diffraction planes of Face Centered Cubic structure. Fourier Transform Infrared spectroscopy demonstrated organic compounds in plant material that mainly involved in reduction. Prunus persica L. Batsch (wild and variety) mediated silver nanoparticles exhibited excellent antibacterial activity against a human bacterial pathogen. The antioxidant activity was also studied that showed effective results on synthesized silver nanoparticles using the 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The result showed that Prunus persica L. Batsch proved to be a useful silver nanoparticle.

scholarly journals Biosynthesis of silver nanoparticles using Haloferax sp. NRS1: image analysis, characterization, in vitro thrombolysis and cytotoxicity

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hend M. Tag ◽  
Amna A. Saddiq ◽  
Monagi Alkinani ◽  
Nashwa Hagagy
Keyword(s):  
Silver Nanoparticles ◽  
Image Analysis ◽  
Biological Activities ◽  
Positive Control ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Vis Spectroscopy ◽  
Negative Surface ◽  
Biosynthesized Agnps

AbstractHaloferax sp strain NRS1 (MT967913) was isolated from a solar saltern on the southern coast of the Red Sea, Jeddah, Saudi Arabia. The present study was designed for estimate the potential capacity of the Haloferax sp strain NRS1 to synthesize (silver nanoparticles) AgNPs. Biological activities such as thrombolysis and cytotoxicity of biosynthesized AgNPs were evaluated. The characterization of silver nanoparticles biosynthesized by Haloferax sp (Hfx-AgNPs) was analyzed using UV–vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The dark brown color of the Hfx-AgNPs colloidal showed maximum absorbance at 458 nm. TEM image analysis revealed that the shape of the Hfx-AgNPs was spherical and a size range was 5.77- 73.14 nm. The XRD spectra showed a crystallographic plane of silver nanoparticles, with a crystalline size of 29.28 nm. The prominent FTIR peaks obtained at 3281, 1644 and 1250 cm− 1 identified the Functional groups involved in the reduction of silver ion reduction to AgNPs. Zeta potential results revealed a negative surface charge and stability of Hfx-AgNPs. Colloidal solution of Hfx-AgNPs with concentrations ranging from 3.125 to 100 μg/mL was used to determine its hemolytic activity. Less than 12.5 μg/mL of tested agent showed no hemolysis with high significant decrease compared with positive control, which confirms that Hfx-AgNPs are considered non-hemolytic (non-toxic) agents according to the ISO/TR 7405-1984(f) protocol. Thrombolysis activity of Hfx-AgNPs was observed in a concentration-dependent manner. Further, Hfx-AgNPs may be considered a promising lead compound for the pharmacological industry.

Development, Characterization and Anticancer Evaluation of Silver Nanoparticles from Dalbergia sissoo Leaf Extracts

2020 ◽  
Vol 16 (2) ◽  
pp. 145-151
Author(s):  
Aakash Deep ◽  
Mitali Verma ◽  
Rakesh K. Marwaha ◽  
Arun K. Sharma ◽  
Beena Kumari
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Activity ◽  
Metal Ion ◽  
Color Change ◽  
Fixed Ratio ◽  
Cancer Cell Line ◽  
Dalbergia Sissoo ◽  
Leaf Extracts ◽  
Standard Drug

Aim: The objective behind this present work is the development and characterization of silver nanoparticles from Dalbergia sissoo leaf extracts and the analysis of anticancer activity. Methods: Silver nanoparticles were prepared by using the aqueous solution of Dalbergia sisoo leaf extract and silver nitrate. The formation of nanoparticles was determined by the color change during the preparation of plant extract to metal ion in a fixed ratio. The prepared nanoparticles were then characterized by TEM, FTIR, DLS, XRD, and SEM. Silver nanoparticles were also evaluated for anticancer activity. Results: Synthesized silver nanoparticles were having good anticancer activity against MCF 7 cancer cell line as compared to the standard drug Doxorubicin. Conclusion: The particle size of nanoparticles was found to lie in the range of 10 to 50 nm.

scholarly journals Phytochemical analysis and antioxidant activity of different plant parts of Pellacalyx axillaris

2019 ◽  
Vol 15 (3) ◽  
pp. 394-397
Author(s):  
Najwa Ahmad Kuthi ◽  
Norazah Basar
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Crude Extracts ◽  
Radical Scavenging Assay

Pellacalyx axillaris or locally known as ‘membuloh’ is a mangrove species belonging to the Rhizophoraceae family. Till date, there has been only one phytochemical study found on this particular plant species and that without any documentation on its biological activities. Therefore, the present work aimed to reveal the phytoconstituents and the antioxidant activity of different crude extracts from different plant parts of P. axillaris. Experimentally, three organic solvents of different polarities i.e. n-hexane, ethyl acetate and methanol were used to prepare the crude extracts from the dried leaves, twigs and barks of P. axillaris. The preliminary phytochemical screening of this species indicated the presence of terpenoids, phenolic compounds, tannins, flavonoids, alkaloids, anthraquinone glycosides and carbohydrates. The in vitro antioxidant activity of the species evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, and ferric reducing antioxidant power (FRAP) suggested that the methanolic bark extract contained potential source of natural antioxidants. Further research into isolation of antioxidant compounds from this species is highly recommended.

scholarly journals Green synthesis of silver nanoparticles using leaf and stem bark extract of Pometia pinnata J.R. Forst & G. Forst

2020 ◽  
Vol 481 ◽  
pp. 012018
Author(s):  
A S Ningrum ◽  
A P Pridyantari ◽  
W Handayani ◽  
K Secario ◽  
D Djuhana ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract

scholarly journals Biochemical and Histo-Anatomical Responses of Lavandula angustifolia Mill. to Spruce and Beech Bark Extracts Application

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 859
Author(s):  
Corneliu Tanase ◽  
Ruxandra Ștefănescu ◽  
Béla Darkó ◽  
Daniela Lucia Muntean ◽  
Anca Corina Fărcaş ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Biological Activities ◽  
Leaf Tissue ◽  
Volatile Oil ◽  
Bark Extract ◽  
Lavandula Angustifolia ◽  
Spruce Bark ◽  
Biological Responses ◽  
Forest Waste ◽  
Pigment Accumulation

This paper aims to assess the biological responses of Lavandula angustifolia Mill. to beech and spruce bark crude extract application. Thus, the biological activity of bark extracts was assessed by determining the germination capacity, biomass production, histo-anatomical aspects and photo-assimilatory pigment accumulation, and by quantitative and qualitative volatile compounds analysis. The application of spruce bark extract (500 mg dry bark/100 mL solvent) resulted in a better development of the leaf tissue and an increase in foliar biomass. We observed the stimulating effect of photo-assimilating pigments accumulation, for all experimental variants, compared to the control. Also, the amount of volatile oil was significantly higher in the treated plants with spruce bark extract (500 mg dry bark/100 mL solvent). Some volatile compounds (cyclen, borneol, cryptone, santalen, and caryophyllene oxide β—farnesene) were identified only in the experimental variants. Also, in the experimental variants, an increase in the quantity of limonene, linalyl acetate and lavandulol was observed. These preliminary results showed that the beech and spruce bark extracts can have biological activities and influence the production of volatile oil in Lavandula angustifolia, causing significant changes in the phytochemical profile of the essential oil. Thus, forest waste bark extracts could be recommended as natural bioregulators in lavender crops.

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