Green Synthesis of Silver Nanoparticles via Phormidium sp. nov. (Cyanophyceae): Amelioration, Characterization and Assessment of the Antibacterial Potential Against Methicillin Resistant Staphylococcus aureus

2021 ◽  
Vol 13 (2) ◽  
pp. 209-216
Author(s):  
Nermin A. El-Semary ◽  
Mona Mabrouk ◽  
Ahmed H. Faraag ◽  
Mona Kilany ◽  
Shymaa H. Omran ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Color Change ◽  
Silver Ions ◽  
Time Saving ◽  
Methicillin Resistant ◽  
Ft Ir ◽  
Ir Analysis ◽  
Stability Results ◽  
Ft Ir Spectroscopy

The use and manufacturing of silver nanoparticles (AgNPs) have recently received considerable attention in many fields. Methicillin resistant Staphylococcus aureus (MRSA) causes infectious diseases in humans. Therefore, the aim of this work was to bio-synthesize AgNPs using the cyanobacteria Phormidium sp. extract and to use this extract with AgNPs to kill MRSA. Extracellular bio-formation of AgNPs by Phormidium sp. was scrutinized at various pH points and temperatures. Then, characterization and assessment of the antimicrobial power of the produced particles were performed. Extract and extract + AgNPs were screened using Fourier Transform Infra-Red (FT-IR) spectroscopy and electron microscopy. The manufacturing and fastness of AgNPs were monitored visually (by color change) and spectroscopically. FT-IR analysis uncovered the biomolecules in the extract that caused the reduction of silver ions, protected them from aggregation and supplied them with high stability. Results indicated that AgNPs are spheres of 9 nm diameter. AgNPs combined with the extract proved to be a powerful antibacterial factor against MRSA. Consequently, biosynthesis of silver nano-spheres using Phormidium sp. with high antagonistic potency against MRSA is considered as an eco-friendly, time-saving strategy and with potential medicinal significance.

scholarly journals Green Synthesis of Silver Nanoparticles from Stem Extracts of Caralluma Fimbriyata and Its Antibacterial Activity

2014 ◽  
Vol 2 (3) ◽  
pp. 305-310
Author(s):  
N. Packialakshmi ◽  
S. Naziya
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Ions ◽  
Zone Of Inhibition ◽  
Plant Parts ◽  
Ft Ir ◽  
Ir Analysis ◽  
Commercial Applications ◽  
Chemical Groups ◽  
Green Synthesized Silver Nanoparticles

The aim of this study was to synthesis of silver nanoparticles in the aqueous stem extracts of Caralluma fimbriyata and investigate its antibacterial activity. Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous stem extracts of plant parts to generate to extremely stable silver nanoparticles in water. The chemical groups studied using FT-IR analysis. Green synthesized silver nanoparticles showed zone of inhibition against isolated gram positive and gram negative bacteria. DOI: http://dx.doi.org/10.3126/ijasbt.v2i3.10796Int J Appl Sci Biotechnol, Vol. 2(3): 305-310  

scholarly journals Investigation of In Vitro Antioxidant and Antibacterial Potential of Silver Nanoparticles Obtained by Biosynthesis Using Beech Bark Extract

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 459 ◽  
Author(s):  
Tanase ◽  
Berta ◽  
Coman ◽  
Roșca ◽  
Man ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Metal Ion ◽  
Color Change ◽  
Vital Role ◽  
Bark Extract ◽  
Ft Ir ◽  
Antioxidant And Antimicrobial Activity ◽  
Average Size

Green synthesis is one of the rapid and best ways for silver nanoparticles (AgNP) synthesis. In the present study, synthesis and bioactivity of AgNPs has been demonstrated using water beech (Fagus sylvatica L.) bark extract. The physical and chemical factors such as time, metal ion solution, and pH, which play a vital role in the AgNPs synthesis, were assessed. The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrometry, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Antioxidant and antimicrobial activity of the obtained AgNPs was evaluated. AgNPs were characterized by color change pattern, and the broad peak obtained at 420–475 nm with UV-Vis confirmed the synthesis of AgNPs. FT-IR results confirmed that phenols and proteins of beech bark extract are mainly responsible for capping and stabilization of synthesized AgNPs. TEM micrographs showed spherical or rarely polygonal and triangular particles with an average size of 32 nm at pH = 9, and 62 nm at pH = 4. Furthermore, synthesized AgNPs were found to exhibit antioxidant activity and have antibacterial effect against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. These results indicate that bark extract of F. sylvatica L. is suitable for synthesizing stable AgNPs, which act as an excellent antimicrobial agent.

scholarly journals Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles

2015 ◽  
Vol 6 ◽  
pp. 2396-2405 ◽  
Author(s):  
Dulce G Romero-Urbina ◽  
Humberto H Lara ◽  
J Jesús Velázquez-Salazar ◽  
M Josefina Arellano-Jiménez ◽  
Eduardo Larios ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Cell Wall ◽  
Molecular Mechanisms ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Ultrastructural Changes ◽  
Future Research ◽  
Methicillin Resistant ◽  
Positively Charged

Silver nanoparticles offer a possible means of fighting antibacterial resistance. Most of their antibacterial properties are attributed to their silver ions. In the present work, we study the actions of positively charged silver nanoparticles against both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. We use aberration-corrected transmission electron microscopy to examine the bactericidal effects of silver nanoparticles and the ultrastructural changes in bacteria that are induced by silver nanoparticles. The study revealed that our 1 nm average size silver nanoparticles induced thinning and permeabilization of the cell wall, destabilization of the peptidoglycan layer, and subsequent leakage of intracellular content, causing bacterial cell lysis. We hypothesize that positively charged silver nanoparticles bind to the negatively charged polyanionic backbones of teichoic acids and the related cell wall glycopolymers of bacteria as a first target, consequently stressing the structure and permeability of the cell wall. This hypothesis provides a major mechanism to explain the antibacterial effects of silver nanoparticles on Staphylococcus aureus. Future research should focus on defining the related molecular mechanisms and their importance to the antimicrobial activity of silver nanoparticles.

Silver Nanoparticles Toxicity and Bactericidal Effect Against Methicillin-Resistant Staphylococcus aureus: Nanoscale Does Matter

2009 ◽  
Vol 5 (1-4) ◽  
pp. 2-9 ◽  
Author(s):  
Nilda Vanesa Ayala-Núñez ◽  
Humberto H. Lara Villegas ◽  
Liliana del Carmen Ixtepan Turrent ◽  
Cristina Rodríguez Padilla
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bactericidal Effect ◽  
Methicillin Resistant ◽  
Nanoparticles Toxicity

Absorbance Linearity and Repeatability in Cylindrical Internal Reflectance FT-IR Spectroscopy of Liquids

1990 ◽  
Vol 44 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Walter M. Doyle
Keyword(s):  
Ir Spectroscopy ◽  
Optical Design ◽  
Experimental Conditions ◽  
Performance Variability ◽  
Ft Ir ◽  
Series Of Experiments ◽  
Ir Analysis ◽  
High Degree ◽  
Internal Reflectance ◽  
Ft Ir Spectroscopy

This paper reports the results of a program aimed at eliminating the sources of performance variability and excessive nonlinearity that have limited the effectiveness of the cylindrical internal reflectance technique for use in quantitative FT-IR analysis. After discussing the various optical factors that can affect performance, it describes a new adjustment-free optical design which promises to eliminate those factors which lead to data unpredictability. Finally, it summarizes the results of a series of experiments carried out to test the effectiveness of the new design. These confirm the achievement of three significant improvements: an enhancement of band strengths by factors of typically 2.5 to 3, significantly improved absorbance linearity, and a high degree of data repeatability under varied experimental conditions.

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