scholarly journals New Insights into the Antioxidant and Anti-Inflammatory Effects of Italian Salvia officinalis Leaf and Flower Extracts in Lipopolysaccharide and Tumor-Mediated Inflammation Models

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 311
Author(s):  
Matteo Brindisi ◽  
Chouaha Bouzidi ◽  
Luca Frattaruolo ◽  
Monica R. Loizzo ◽  
Maria Stella Cappello ◽  
...  
Keyword(s):  
Current Knowledge ◽  
Salvia Officinalis ◽  
Inflammatory Activity ◽  
Neoplastic Progression ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Salvia Officinalis L

This work aimed to investigate and compare the in vitro antioxidant and anti-inflammatory effects of Salvia officinalis L. (sage) from Italy, with the aim of raising its current knowledge in this field. Leaves and flowers (S1–S8), harvested in two areas of Southern Italy, were extracted with methanol as a solvent by maceration or ultrasound-assisted extraction. Sage extracts, analysed by high pressure liquid chromatography-diode-array detection-electrospray ionization-quadrupole-mass spectroscopy (HPLC-DAD-ESI-Q-MS), exerted a promising antioxidant activity investigated using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and β-carotene bleaching tests, and elicited a significant decrease in reactive oxygen species (ROS) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages. The anti-inflammatory activity was analysed in the same in vitro model. All the extracts did not affect cell viability although they showed anti-inflammatory activity, as they induced a decrease in nitrite levels that was greater than 50%, when employed at 50 µg/mL. Furthermore, they elicited a decrease in nitrite levels, as well as a decline in pro-inflammatory cytokine expression. The NF-κB transcription factor proved to be involved in the mechanisms that underlie such effects. Interestingly, sage extracts were able to interfere with the inflammatory activity induced by breast cancer cell-conditioned media (nitrite levels were significantly decreased, p < 0.05; p < 0.01), highlighting for the first time the important role of S. officinalis in controlling inflammation processes related to neoplastic progression.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5295
Author(s):  
Xinyu Zhao ◽  
Ruyi Chen ◽  
Yueyue Shi ◽  
Xiaoxi Zhang ◽  
Chongmei Tian ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preparative Hplc ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

This study aimed to isolate, prepare and identify the main flavonoids from a standardized Smilax glabra flavonoids extract (SGF) using preparative HPLC, MS, 1H NMR and 13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+ radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p < 0.01) and the protein expression of NF-κB p-p65 (p < 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids in S. glabra.

scholarly journals Essential Oil of Cymbopogon Citratus Grown in Umuahia: A Viable Candidate for Anti-Inflammatory and Antioxidant Therapy

2021 ◽  
Vol 25 (1) ◽  
pp. 1-14
Author(s):  
Uchechi Bliss Onyedikachi ◽  
Favour Matthew Awah ◽  
Charles Nnanna Chukwu ◽  
Emmanuel Ejiofor
Keyword(s):  
Essential Oil ◽  
Vitamin C ◽  
Therapeutic Potential ◽  
Preliminary Evidence ◽  
Gas Chromatography Mass Spectrometry ◽  
Cymbopogon Citratus ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

Abstract The essential oils of Cymbopogon citratus (EOCC) has found use in medicine, food and chemical industry. This study attempts to provide evidence of its suitability for antioxidant and anti-inflammatory therapy. Total phenol and total flavonoid of EOCC was 49.83±0.39mg GAE/g of extract and 352.82±3.45 µg QEC/g of extract respectively. Gas chromatography-mass spectrometry (GC-MS) analysis of its essential oil (EOCC) showed 25 peaks with myrcenyl acetate (9.703%), caryophyllene (8.997%), citronella (6.383%) been the most abundant. The in vitro anti-inflammatory assay using human red blood cell (HRBC) membrane stabilization shows that at 200µg/mL, the percentage inhibition of EOCC was significantly higher compared to diclofenac both for heat-induced and hypotonic induced haemolysis. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed a comparable and dose-dependent increase from 50 to 400 μg/mL in relation to vitamin C. Half maximal inhibitory concentration (IC50) of EOCC (73.16±12.89 μg/mL and 656.01±0.01 μmol Fe (II)/L) was remarkably higher compared to that of vitamin C (69.09±4.52 μg/mL and 246.79±0.01 μmol Fe (II)/L) both for DPPH and FRAP assays respectively. In conclusion, results from this study establish preliminary evidence on the therapeutic potential of EOCC in managing inflammation and oxidative stress caused by free radicals.

scholarly journals Screening of In Vitro Health Benefits of Tangerine Tomatoes

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 230 ◽  
Author(s):  
Hartono Tanambell ◽  
Siew Young Quek ◽  
Karen Suzanne Bishop
Keyword(s):  
Scientific Evidence ◽  
Health Benefits ◽  
Beta Carotene ◽  
Antioxidant Power ◽  
Chicken Egg ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Prevention Of Cancer

Tomatoes have been associated with various health benefits, including the prevention of chronic diseases. The cis-isomers of lycopene occurring in tangerine tomatoes were, through clinical trials, proven to be more bioavailable than the all-trans lycopene found in red tomatoes. Nonetheless, scientific evidence regarding the bioactivities of the tangerine tomatoes is lacking. In this article, the antioxidant, anticancer, and anti-inflammatory properties of extracts prepared from four different tomato varieties, namely Alfred, Olga’s Round Golden Chicken Egg, Golden Green, and Golden Eye, were investigated. While the antioxidant capacities of the extracts were measured through the ferric reducing antioxidant power (FRAP) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, their anti-proliferative properties in prostate cancer cell lines were examined through the Sulforhodamine-B (SRB) assay. The anti-inflammatory activities of the extracts were assessed through the toll-like receptor (TLR)2, TLR4, and nucleotide-binding oligomerization domain containing protein 2 (NOD2)-mediated inflammatory pathways. Our results show that the tangerine tomatoes had lower IC50 values in both the anticancer and anti-inflammatory assays compared to the red tomatoes. Specifically, the half-maximal inhibitory concentration (IC50) values of the tangerine tomatoes in LNCaP cells were approximately two to three fold lower than the red tomato (IC50: 14.46, 5.62, and 8.08 mg dry tomato equivalent/mL from Alfred hexane-acetone, Olga’s Round Golden Chicken Egg hexane, and Golden Green hexane, respectively). These findings indicate that the tangerine varieties, Olga’s Round Golden Chicken Egg and Golden Green, possess greater potential to be used in conjunction with treatment and for the prevention of cancer and inflammatory-related diseases than the Alfred (red) and Golden Eye (high beta-carotene) varieties.

scholarly journals Chemical Profile, Antioxidant, Anti-Inflammatory, and Anti-Cancer Effects of Italian Salvia rosmarinus Spenn. Methanol Leaves Extracts

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 826
Author(s):  
Matteo Brindisi ◽  
Chouaha Bouzidi ◽  
Luca Frattaruolo ◽  
Monica R. Loizzo ◽  
Rosa Tundis ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Nitrite Production ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Anti Inflammatory

In this study, we evaluated and compared the chemical composition, the antioxidant, anti-inflammatory, and anti-proliferative effects of four methanol extracts (R1–R4), of Salvia rosmarinus Spenn. in two different sites of Southern Italy obtained by maceration or ultrasound-assisted extraction. Extracts of S. rosmarinus collected on the Ionian coast are indicated with the abbreviations R1 (maceration) and R2 (ultrasound-assisted extraction). Extracts of S. rosmarinus collected on the Tyrrhenian coast are indicated with the abbreviations R3 (maceration) and R4 (ultrasound-assisted extraction). The chemical composition was analyzed using High Pressure liquid chromatography–Diod-Array detection–Electrospray ionization–Quadrupole–Mass Spectroscopy (HPLC-DAD-ESI-Q-MS). The antioxidant activity was analyzed by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and Ferric Reducing Antioxidant Power (FRAP) assays. Antioxidant features were also assessed in lipopolysaccharide (LPS)-stimulated RAW-264.7 murine macrophages, evaluating Reactive Oxygen Species (ROS) production; in the same experimental model, the anti-inflammatory activity of the extracts was investigated. Interestingly, all extracts displayed antioxidant and anti-inflammatory properties. They exhibited significative nitrite production inhibitory activity, whith IC50 values ranging from 3.46 to 5.53 µg/mL, without impairing cell viability. The anti-inflammatory activity was also investigated by Western Blotting and immunofluorescence assay, highlighting the R3 and R4 extracts ability to reduce NF-κB translocation, as well as to disrupt the MAPKs signaling pathway. Extracts exhibited both potential anti-proliferative activity on breast cancer cells, inducing apoptosis, without affecting non-tumorigenic cells, and the ability to inhibit MDA-MB-231 cells’ motility. Finally, the rosemary extracts treatment significantly reduced the power of conditioned media, from MCF-7 or MDA-MB-231 cells to induce nitrite production on RAW 264.7 cells, confirming their promising anti-inflammatory activity.

scholarly journals Assessment of Immunomodulatory Effect on Natural Antioxidants – An In Vitro Study

2021 ◽  
Vol 10 (42) ◽  
pp. 3612-3616
Author(s):  
Deepigaa Manivasagam ◽  
Arvind Muthukrishnan ◽  
Rajeshkumar S.
Keyword(s):  
In Vitro Study ◽  
Natural Antioxidants ◽  
Vitro Study ◽  
Immunomodulatory Effect ◽  
Safe Alternative ◽  
Antioxidant Power ◽  
Immune Mediated ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

BACKGROUND Garlic has anti allergic, anti-tumour and chemo preventive effect. Turmeric has analgesic, anti-inflammatory, wound healing, and immunomodulatory activities. Spirulina has antioxidant, immunomodulatory, anti-inflammatory, anticancer, antiviral, and antibacterial effects. Honey has antimicrobial, antioxidant, anticancer, antiinflammatory. The purpose of this study was to prepare herbal formulations of garlic, turmeric, honey, and spirulina and analyse its immunomodulatory effect. METHODS Garlic, turmeric, honey, and spirulina were combined with 100 ml of distilled water and simmered for 15 minutes before being filtered and condensed again to 5 ml. The diphenyl 1-picryl-hydrazyl-hydrate test (DPPH), ferric reducing antioxidant power assay (FRAP) and H2O2 assays were used to evaluate the immunomodulatory effect. RESULTS The prepared extract showed significant results at 50 l when assessed using DPPH, FRAP and H2O2 assay (i.e.) 72.1 %, 94.6 % and 67.9 % respectively. CONCLUSIONS This in-vitro study shows that the combination of garlic, turmeric, honey and spirulina formulation have good immunomodulatory effects when compared to the standard. Hence, in future, it can be used as a safe alternative instead of commercially available medicines in the form of in-situ gel for management of immune mediated disorders. KEY WORDS Garlic, Turmeric, Honey, Spirulina, Antioxidant, Immunomodulator

Topical anti-inflammatory activity of Salvia officinalis L. leaves: the relevance of ursolic acid

2001 ◽  
Vol 75 (2-3) ◽  
pp. 125-132 ◽  
Author(s):  
D. Baricevic ◽  
S. Sosa ◽  
R. Della Loggia ◽  
A. Tubaro ◽  
B. Simonovska ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Salvia Officinalis ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
Salvia Officinalis L

scholarly journals Antioxidant, anti-inflammatory and cytotoxic activities of the unsaponifiable fraction of extra virgin olive oil

2020 ◽  
Vol 71 (4) ◽  
pp. 386
Author(s):  
M. M. Elaasser ◽  
M. K.S. Morsi ◽  
S. M. Galal ◽  
M. K. Abd El-Rahman ◽  
M. A. Katry
Keyword(s):  
Olive Oil ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Unsaponifiable Fraction ◽  
Antioxidant Power ◽  
Nitric Oxide Inhibition ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Oxide Inhibition

The health benefits of olive oil are well-known. In this study, the unsaponifiable fraction of extra virgin olive oil (Unsap) was investigated for reducing power capacity, ferric reducing antioxidant power, fer-rous chelating activity and nitric oxide inhibition. The present study was also designed to evaluate the in vitro cytotoxic effect of the Unsap against human carcinoma cells. The anti-inflammatory potential of Unsap has been determined via the inhibition of Human Cyclooxygenases. The results showed that Unsap is efficient for ferric reducing antioxidant power and nitric oxide inhibition. Unsap has a selective effect as anti-inflammatory agent. The results showed moderate to good in vitro antitumor activities of Unsap against human liver, lung and pancreas cancer cells with IC50 ranging from 19.6 to 30.4 μg/mL and good selectivity index (≥ 2). In conclusion, Unsap represents a promising and safe antitumor and antioxidant material that supports the need for further investigation.

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO BIOLOGICAL CHARACTERIZATION OF AQUEOUS AND METHANOLIC EXTRACT OF BACOPA MONNIERI

2016 ◽  
Vol 8 (12) ◽  
pp. 90
Author(s):  
Romana Parveen ◽  
Tooba Naz Shamsi ◽  
Himanshu Kumar ◽  
Sadaf Fatima
Keyword(s):  
Bacterial Infections ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bacopa Monnieri ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Antioxidant Power ◽  
Anti Inflammatory

<p><strong>Objective</strong>:<strong> </strong>The present study was designed to identify the phytocompounds, to compare the antibacterial, antioxidant, and anti-inflammatory effects of aqueous and methanolic extract of <em>Bacopa monnieri</em>.</p><p><strong>Methods</strong>:<strong> </strong>Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), Super oxide dismutase (SOD), Reduced glutathione (GSH), Catalase assays. Anti-inflammatory activity was measured with inhibition of albumin denaturation and trypsin inhibitory assay. Finally, extracts were tested against various pathogenic bacterial and fungal strains by broth dilution assay and disc diffusion assay respectively.</p><p><strong>Results: </strong>Results showed the presence of alkaloids, flavonoids, phenols, quinines and glycosides etc while steroids and carboxylic acid were absent. The extracts demonstrated free radical-scavenging activity quite comparable with standard ascorbic acid. Methanolic extract exerted comparative higher antioxidant and anti-inflammatory activity than aqueous extract. Both extracts were most effective against <em>Bacillus subtilis</em><em> </em>and lowest inhibition against<em> Staphylococcus aureus</em>.</p><p><strong>Conclusion: </strong>The results obtained clearly indicated a promising potential of <em>B. monnieri</em> for serving as a strong ROS scavenger, might be used as anti-arthritic and strong natural antibiotic agent for effective treatment of various oxidative stressed disorders (cancer, cardiovascular diseases), inflammatory disorders (rheumatoid arthritis) and various bacterial infections.</p>

scholarly journals Preliminary Screening for Anti-Inflammatory, Anti-Nociceptive and Antioxidant Activities of Five New Synthesized Paratoluene Sulphonamide Derivatives of Amino Acids

2016 ◽  
Vol 5 ◽  
pp. 74-78
Author(s):  
Chinyere Benardette Chinaka Ikpa ◽  
U.C. Okoro ◽  
Collins I. Ubochi ◽  
Kieran O. Nwanorh ◽  
Samuel O. Onoja
Keyword(s):  
Amino Acids ◽  
Antioxidant Activities ◽  
Tail Flick ◽  
Antioxidant Power ◽  
Preliminary Screening ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Dpph Scavenging ◽  
Derivatives Of

The anti-inflammatory, antinociceptive and antioxidant activities of five new synthesized paratoluene sulphonamide derivatives of amino acids were evaluated in this study. The anti-inflammatory and antinociceptive was evaluated using egg albumin induced paw edema and tail flick rat model respectively, at the dose of 400 mg/kg; while the antioxidant activity was evaluatedin vitrousing 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) scavenging and Ferric Reducing Antioxidant Power assay. The compounds produced various degrees of anti-inflammatory and antinociceptive activities but did not produce antioxidant effect. The sulphonamide derivative has anti-inflammatory and antinociceptive properties and can be used as drug for this purpose.

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