scholarly journals Assessment of Immunomodulatory Effect on Natural Antioxidants – An In Vitro Study

2021 ◽  
Vol 10 (42) ◽  
pp. 3612-3616
Author(s):  
Deepigaa Manivasagam ◽  
Arvind Muthukrishnan ◽  
Rajeshkumar S.
Keyword(s):  
In Vitro Study ◽  
Natural Antioxidants ◽  
Vitro Study ◽  
Immunomodulatory Effect ◽  
Safe Alternative ◽  
Antioxidant Power ◽  
Immune Mediated ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

BACKGROUND Garlic has anti allergic, anti-tumour and chemo preventive effect. Turmeric has analgesic, anti-inflammatory, wound healing, and immunomodulatory activities. Spirulina has antioxidant, immunomodulatory, anti-inflammatory, anticancer, antiviral, and antibacterial effects. Honey has antimicrobial, antioxidant, anticancer, antiinflammatory. The purpose of this study was to prepare herbal formulations of garlic, turmeric, honey, and spirulina and analyse its immunomodulatory effect. METHODS Garlic, turmeric, honey, and spirulina were combined with 100 ml of distilled water and simmered for 15 minutes before being filtered and condensed again to 5 ml. The diphenyl 1-picryl-hydrazyl-hydrate test (DPPH), ferric reducing antioxidant power assay (FRAP) and H2O2 assays were used to evaluate the immunomodulatory effect. RESULTS The prepared extract showed significant results at 50 l when assessed using DPPH, FRAP and H2O2 assay (i.e.) 72.1 %, 94.6 % and 67.9 % respectively. CONCLUSIONS This in-vitro study shows that the combination of garlic, turmeric, honey and spirulina formulation have good immunomodulatory effects when compared to the standard. Hence, in future, it can be used as a safe alternative instead of commercially available medicines in the form of in-situ gel for management of immune mediated disorders. KEY WORDS Garlic, Turmeric, Honey, Spirulina, Antioxidant, Immunomodulator

Anti-inflammatory properties of antipsychotic drugs via their effect on cytokine production – in vitro study

2013 ◽  
Vol 65 ◽  
pp. 128
Author(s):  
Ewa Obuchowicz ◽  
Anna M. Bielecka ◽  
Monika Paul-Samojedny ◽  
Marta Nowacka
Keyword(s):  
Antipsychotic Drugs ◽  
Cytokine Production ◽  
In Vitro Study ◽  
Vitro Study ◽  
Anti Inflammatory

scholarly journals Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in vitro study using baicalein

2020 ◽  
Vol 14 ◽  
pp. 177-183
Author(s):  
Chang-Chin Wu ◽  
Yi-Ru Chen ◽  
Dai-Hua Lu ◽  
Li-Ho Hsu ◽  
Kai-Chiang Yang ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Post Treatment ◽  
Vitro Study ◽  
Osteoarthritic Chondrocytes ◽  
Anti Inflammatory

scholarly journals Evaluation of In Vitro Antioxidant and Antidiabetic Activities of Aristolochia longa Extracts

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Nasreddine El Omari ◽  
Karima Sayah ◽  
Saad Fettach ◽  
Omar El Blidi ◽  
Abdelhakim Bouyahya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Inhibitory Effect ◽  
Phytochemical Analysis ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Therapeutic Tools

Oxidative stress plays a major role in diabetic physiopathology; hence, the interest of using natural antioxidants as therapeutic tools exists. The aim of this study was the evaluation of in vitro antioxidant activity and inhibitory potential of organic extracts from Aristolochia longa roots against key enzymes linked to hyperglycemia. Antioxidant activity was performed using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals and ferric reducing/antioxidant power (FRAP) methods. The α-Glucosidase and β-Galactosidase inhibitory activities were investigated using an in vitro model. Moreover, phytochemical analysis of tested extracts was carried out. The aqueous fraction of this herb exhibited the highest antioxidant activity for both DPPH and ABTS methods, IC50=125.40±2.40 μg/mL and IC50=65.23±2.49 μg/mL, respectively. However, the ethyl acetate fraction possessed the strongest inhibitory effect towards α-Glucosidase (IC50=1.112±0.026 mg/mL). Furthermore, the result showed high levels of phenolic content. The results showed that this plant could be a significant source of medically important natural compounds.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5295
Author(s):  
Xinyu Zhao ◽  
Ruyi Chen ◽  
Yueyue Shi ◽  
Xiaoxi Zhang ◽  
Chongmei Tian ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preparative Hplc ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

This study aimed to isolate, prepare and identify the main flavonoids from a standardized Smilax glabra flavonoids extract (SGF) using preparative HPLC, MS, 1H NMR and 13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+ radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p < 0.01) and the protein expression of NF-κB p-p65 (p < 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids in S. glabra.

In vitro study of iron coordination properties, anti-inflammatory potential, and cytotoxic effects of N-salicylidene and N-vanillidene anil Schiff bases

2018 ◽  
Vol 72 (9) ◽  
pp. 2171-2180 ◽  
Author(s):  
Zorica D. Petrović ◽  
Jelena Đorović ◽  
Dušica Simijonović ◽  
Snežana Trifunović ◽  
Vladimir P. Petrović
Keyword(s):  
Schiff Bases ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cytotoxic Effects ◽  
Coordination Properties ◽  
Iron Coordination ◽  
Anti Inflammatory

scholarly journals Immunomodulatory Effect of Gymnema sylvestre (R.Br.) Leaf Extract: An In Vitro Study in Rat Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139631 ◽  
Author(s):  
Vineet Kumar Singh ◽  
Padmanabh Dwivedi ◽  
B. R. Chaudhary ◽  
Ramesh Singh
Keyword(s):  
Rat Model ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Immunomodulatory Effect ◽  
Gymnema Sylvestre
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