scholarly journals The XPF-ERCC1 Complex Is Essential for Genome Stability and Is Involved in the Mechanism of Gene Targeting in Physcomitrella patens

2019 ◽  
Vol 10 ◽  
Author(s):  
Anouchka Guyon-Debast ◽  
Patricia Rossetti ◽  
Florence Charlot ◽  
Aline Epert ◽  
Jean-Marc Neuhaus ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Genome Stability ◽  
Physcomitrella Patens

scholarly journals The Importance of ATM and ATR in Physcomitrella patens DNA Damage Repair, Development, and Gene Targeting

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 752
Author(s):  
Martin Martens ◽  
Ralf Horres ◽  
Edelgard Wendeler ◽  
Bernd Reiss
Keyword(s):  
Dna Damage ◽  
Gene Targeting ◽  
Genome Stability ◽  
Physcomitrella Patens ◽  
Dna Damage Repair ◽  
Transcriptional Response ◽  
Vegetative Development ◽  
Dsb Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Damage Repair

Coordinated by ataxia-telangiectasia-mutated (ATM) and ATM and Rad3-related (ATR), two highly conserved kinases, DNA damage repair ensures genome integrity and survival in all organisms. The Arabidopsis thaliana (A. thaliana) orthologues are well characterized and exhibit typical mammalian characteristics. We mutated the Physcomitrella patens (P. patens) PpATM and PpATR genes by deleting functionally important domains using gene targeting. Both mutants showed growth abnormalities, indicating that these genes, particularly PpATR, are important for normal vegetative development. ATR was also required for repair of both direct and replication-coupled double-strand breaks (DSBs) and dominated the transcriptional response to direct DSBs, whereas ATM was far less important, as shown by assays assessing resistance to DSB induction and SuperSAGE-based transcriptomics focused on DNA damage repair genes. These characteristics differed significantly from the A. thaliana genes but resembled those in yeast (Saccharomyces cerevisiae). PpATR was not important for gene targeting, pointing to differences in the regulation of gene targeting and direct DSB repair. Our analysis suggests that ATM and ATR functions can be substantially diverged between plants. The differences in ATM and ATR reflect the differences in DSB repair pathway choices between A. thaliana and P. patens, suggesting that they represent adaptations to different demands for the maintenance of genome stability.

scholarly journals MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens

2011 ◽  
Vol 40 (8) ◽  
pp. 3496-3510 ◽  
Author(s):  
Yasuko Kamisugi ◽  
Didier G. Schaefer ◽  
Jaroslav Kozak ◽  
Florence Charlot ◽  
Nathalie Vrielynck ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens

scholarly journals The NHEJ Repair of DNA Double Strand Breaks in Physcomitrella patens Depends on the Kleisin NSE4 of the SMC5/6 Complex

2020 ◽  
Author(s):  
Radka Vágnerová ◽  
Marcela Holá ◽  
Karel J. Angelis
Keyword(s):  
Genome Stability ◽  
Physcomitrella Patens ◽  
Genotoxic Stress ◽  
Single Copy ◽  
Insertion Mutagenesis ◽  
Dna Double Strand Breaks ◽  
Dsb Repair ◽  
Strand Breaks ◽  
Circular Structure ◽  
Repair Defect

AbstractStructural maintenance of chromosomes (SMC) complexes are involve in cohesion, condensation and maintenance of genome stability. Based on the sensitivity of mutants to genotoxic stress the SMC5/6 complex is thought to play imminent role in DNA stabilization during repair by encircling DNA at the site of lesion by bridging the heteroduplex of SMC5 and SMC6 by non SMC kleisin components NSE1, 3 and 4. In this study, we tested how formation of the SMC5/6 circular structure affects mutant sensitivity to genotoxic stress, kinetics of DSB repair and insertion mutagenesis. In the moss Physcomitrella patens SMC6 and NSE4 are essential single copy genes and this is why we used blocking of transcription to reveal their mutated phenotype. Even slight attenuation of transcription by dCas9 binding was enough to obtain stable lines with DSB repair defect and specific bleomycin sensitivity. Whereas survival after bleomycin or MMS treatment fully depends on active SMC6, NSE4 has little or negligible effect. We conclude that whereas circularization of SMC5/6 provided by the kleisin NSE4 is indispensable for the immediate NHEJ DSB repair response, other functions associated with SMC5/6 complex are critical to survive DNA damage.

Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

2010 ◽  
pp. 273-290 ◽  
Author(s):  
Alison W. Roberts ◽  
Christos S. Dimos ◽  
Michael J. Budziszek ◽  
Chessa A. Goss ◽  
Virginia Lai
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens

RAD51 loss of function abolishes gene targeting and de-represses illegitimate integration in the moss Physcomitrella patens

DNA Repair ◽  
2010 ◽  
Vol 9 (5) ◽  
pp. 526-533 ◽  
Author(s):  
D.G. Schaefer ◽  
F. Delacote ◽  
F. Charlot ◽  
N. Vrielynck ◽  
A. Guyon-Debast ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens ◽  
Loss Of Function

scholarly journals Factors Affecting Organelle Genome Stability in Physcomitrella patens

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 145 ◽  
Author(s):  
Masaki Odahara
Keyword(s):  
Genome Stability ◽  
Physcomitrella Patens ◽  
Genome Structure ◽  
Land Plant ◽  
Factors Affecting ◽  
Organelle Genome ◽  
Organelle Genomes ◽  
Recombination Repair ◽  
The Relationship ◽  
Bacterial Type

Organelle genomes are essential for plants; however, the mechanisms underlying the maintenance of organelle genomes are incompletely understood. Using the basal land plant Physcomitrella patens as a model, nuclear-encoded homologs of bacterial-type homologous recombination repair (HRR) factors have been shown to play an important role in the maintenance of organelle genome stability by suppressing recombination between short dispersed repeats. In this review, I summarize the factors and pathways involved in the maintenance of genome stability, as well as the repeats that cause genomic instability in organelles in P. patens, and compare them with findings in other plant species. I also discuss the relationship between HRR factors and organelle genome structure from the evolutionary standpoint.

Cloning of the PpMSH-2 cDNA of Physcomitrella patens, a moss in which gene targeting by homologous recombination occurs at high frequency

Biochimie ◽  
2001 ◽  
Vol 83 (11-12) ◽  
pp. 1003-1008 ◽  
Author(s):  
Florent Brun ◽  
Martine Gonneau ◽  
Marie-Pascale Doutriaux ◽  
Michel Laloue ◽  
Fabien Nogué
Keyword(s):  
Homologous Recombination ◽  
Gene Targeting ◽  
High Frequency ◽  
Physcomitrella Patens

scholarly journals RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens

2018 ◽  
Vol 30 (3) ◽  
pp. 717-736 ◽  
Author(s):  
Gertrud Wiedemann ◽  
Nico van Gessel ◽  
Fabian Köchl ◽  
Lisa Hunn ◽  
Katrin Schulze ◽  
...  
Keyword(s):  
Dna Repair ◽  
Gene Targeting ◽  
Physcomitrella Patens ◽  
Recq Helicases
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