scholarly journals Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens

2005 ◽  
Vol 33 (19) ◽  
pp. e173-e173 ◽  
Author(s):  
Y. Kamisugi
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens

scholarly journals MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens

2011 ◽  
Vol 40 (8) ◽  
pp. 3496-3510 ◽  
Author(s):  
Yasuko Kamisugi ◽  
Didier G. Schaefer ◽  
Jaroslav Kozak ◽  
Florence Charlot ◽  
Nathalie Vrielynck ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens

scholarly journals The Importance of ATM and ATR in Physcomitrella patens DNA Damage Repair, Development, and Gene Targeting

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 752
Author(s):  
Martin Martens ◽  
Ralf Horres ◽  
Edelgard Wendeler ◽  
Bernd Reiss
Keyword(s):  
Dna Damage ◽  
Gene Targeting ◽  
Genome Stability ◽  
Physcomitrella Patens ◽  
Dna Damage Repair ◽  
Transcriptional Response ◽  
Vegetative Development ◽  
Dsb Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Damage Repair

Coordinated by ataxia-telangiectasia-mutated (ATM) and ATM and Rad3-related (ATR), two highly conserved kinases, DNA damage repair ensures genome integrity and survival in all organisms. The Arabidopsis thaliana (A. thaliana) orthologues are well characterized and exhibit typical mammalian characteristics. We mutated the Physcomitrella patens (P. patens) PpATM and PpATR genes by deleting functionally important domains using gene targeting. Both mutants showed growth abnormalities, indicating that these genes, particularly PpATR, are important for normal vegetative development. ATR was also required for repair of both direct and replication-coupled double-strand breaks (DSBs) and dominated the transcriptional response to direct DSBs, whereas ATM was far less important, as shown by assays assessing resistance to DSB induction and SuperSAGE-based transcriptomics focused on DNA damage repair genes. These characteristics differed significantly from the A. thaliana genes but resembled those in yeast (Saccharomyces cerevisiae). PpATR was not important for gene targeting, pointing to differences in the regulation of gene targeting and direct DSB repair. Our analysis suggests that ATM and ATR functions can be substantially diverged between plants. The differences in ATM and ATR reflect the differences in DSB repair pathway choices between A. thaliana and P. patens, suggesting that they represent adaptations to different demands for the maintenance of genome stability.

Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

2010 ◽  
pp. 273-290 ◽  
Author(s):  
Alison W. Roberts ◽  
Christos S. Dimos ◽  
Michael J. Budziszek ◽  
Chessa A. Goss ◽  
Virginia Lai
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens

RAD51 loss of function abolishes gene targeting and de-represses illegitimate integration in the moss Physcomitrella patens

DNA Repair ◽  
2010 ◽  
Vol 9 (5) ◽  
pp. 526-533 ◽  
Author(s):  
D.G. Schaefer ◽  
F. Delacote ◽  
F. Charlot ◽  
N. Vrielynck ◽  
A. Guyon-Debast ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens ◽  
Loss Of Function

Cloning of the PpMSH-2 cDNA of Physcomitrella patens, a moss in which gene targeting by homologous recombination occurs at high frequency

Biochimie ◽  
2001 ◽  
Vol 83 (11-12) ◽  
pp. 1003-1008 ◽  
Author(s):  
Florent Brun ◽  
Martine Gonneau ◽  
Marie-Pascale Doutriaux ◽  
Michel Laloue ◽  
Fabien Nogué
Keyword(s):  
Homologous Recombination ◽  
Gene Targeting ◽  
High Frequency ◽  
Physcomitrella Patens

scholarly journals RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens

2018 ◽  
Vol 30 (3) ◽  
pp. 717-736 ◽  
Author(s):  
Gertrud Wiedemann ◽  
Nico van Gessel ◽  
Fabian Köchl ◽  
Lisa Hunn ◽  
Katrin Schulze ◽  
...  
Keyword(s):  
Dna Repair ◽  
Gene Targeting ◽  
Physcomitrella Patens ◽  
Recq Helicases

Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

2020 ◽  
pp. 125-144
Author(s):  
Alison W. Roberts ◽  
Christos S. Dimos ◽  
Michael J. Budziszek ◽  
Chessa A. Goss ◽  
Virginia Lai ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens

scholarly journals Efficient gene targeting in the moss Physcomitrella patens

1997 ◽  
Vol 11 (6) ◽  
pp. 1195-1206 ◽  
Author(s):  
Didier G. Schaefer ◽  
Jean-Pierre Zryd
Keyword(s):  
Gene Targeting ◽  
Physcomitrella Patens ◽  
Efficient Gene

scholarly journals The mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration

2006 ◽  
Vol 34 (21) ◽  
pp. 6205-6214 ◽  
Author(s):  
Yasuko Kamisugi ◽  
Katja Schlink ◽  
Stefan A. Rensing ◽  
Gabriele Schween ◽  
Mark von Stackelberg ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Gene Targeting ◽  
Physcomitrella Patens ◽  
Multiple Integration
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