scholarly journals Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation

2016 ◽  
Vol 3 ◽  
Author(s):  
Austin T. Mudd ◽  
Jaime Salcedo ◽  
Lindsey S. Alexander ◽  
Stacey K. Johnson ◽  
Caitlyn M. Getty ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Milk Oligosaccharides ◽  
Porcine Milk

scholarly journals Characterization of porcine milk oligosaccharides over lactation between primiparous and multiparous female pigs

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinhua Wei ◽  
Zhuo A. Wang ◽  
Bing Wang ◽  
Marefa Jahan ◽  
Zhongfu Wang ◽  
...  
Keyword(s):  
Milk Oligosaccharides ◽  
Porcine Milk ◽  
Multiparous Female

Microbial production of sialic acid and sialylated human milk oligosaccharides: Advances and perspectives

2019 ◽  
Vol 37 (5) ◽  
pp. 787-800 ◽  
Author(s):  
Xiaolong Zhang ◽  
Yanfeng Liu ◽  
Long Liu ◽  
Jianghua Li ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Human Milk ◽  
Microbial Production ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides

4-O-Acetyl-sialic acid (Neu4,5Ac2) in acidic milk oligosaccharides of the platypus (Ornithorhynchus anatinus) and its evolutionary significance

Glycobiology ◽  
2015 ◽  
Vol 25 (6) ◽  
pp. 683-697 ◽  
Author(s):  
Tadasu Urashima ◽  
Hiroaki Inamori ◽  
Kenji Fukuda ◽  
Tadao Saito ◽  
Michael Messer ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Evolutionary Significance ◽  
Milk Oligosaccharides ◽  
Ornithorhynchus Anatinus

scholarly journals Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Daniel Garrido ◽  
Santiago Ruiz-Moyano ◽  
Danielle G. Lemay ◽  
David A. Sela ◽  
J. Bruce German ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Bifidobacterium Longum ◽  
Gene Clusters ◽  
Bifidobacterium Bifidum ◽  
Rna Seq ◽  
Human Milk Oligosaccharides ◽  
Orthologous Genes ◽  
Milk Oligosaccharides ◽  
Close Relationship ◽  
Utilization Patterns

Abstract Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria.

scholarly journals Structural Determination and Daily Variations of Porcine Milk Oligosaccharides

2010 ◽  
Vol 58 (8) ◽  
pp. 4653-4659 ◽  
Author(s):  
Nannan Tao ◽  
Karen L. Ochonicky ◽  
J. Bruce German ◽  
Sharon M. Donovan ◽  
Carlito B. Lebrilla
Keyword(s):  
Structural Determination ◽  
Milk Oligosaccharides ◽  
Daily Variations ◽  
Porcine Milk

scholarly journals Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing 4-O-acetyl sialic acid

2016 ◽  
Vol 14 (36) ◽  
pp. 8586-8597 ◽  
Author(s):  
Hai Yu ◽  
Jie Zeng ◽  
Yanhong Li ◽  
Vireak Thon ◽  
Baojun Shi ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Chemoenzymatic Synthesis ◽  
Milk Oligosaccharides ◽  
One Pot

Chemoenzymatic synthesis: Monotreme milk glycans and other sialosides containing a 4-O-acetyl-sialic acid were synthesized in a gram or preparative scales using a one-pot two-enzyme sialylation system containing bacterial CMP-sialic acid synthetase and sialyltransferase PmST3.

scholarly journals In Vitro Fermentation of Porcine Milk Oligosaccharides and Galacto-oligosaccharides Using Piglet Fecal Inoculum

2016 ◽  
Vol 64 (10) ◽  
pp. 2127-2133 ◽  
Author(s):  
Elisabetta Difilippo ◽  
Feipeng Pan ◽  
Madelon Logtenberg ◽  
Rianne (H.A.M.) Willems ◽  
Saskia Braber ◽  
...  
Keyword(s):  
Milk Oligosaccharides ◽  
In Vitro Fermentation ◽  
Porcine Milk

scholarly journals Human milk oligosaccharides inhibit rotavirus infectivity in vitro and in acutely infected piglets

2013 ◽  
Vol 110 (7) ◽  
pp. 1233-1242 ◽  
Author(s):  
Shelly N. Hester ◽  
Xin Chen ◽  
Min Li ◽  
Marcia H. Monaco ◽  
Sarah S. Comstock ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Human Milk ◽  
Mrna Expression ◽  
Viral Infectivity ◽  
Structural Protein ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
Midline Laparotomy

Human milk (HM) is rich in oligosaccharides (HMO) that exert prebiotic and anti-infective activities. HM feeding reduces the incidence of rotavirus (RV) infection in infants. Herein, the anti-RV activity of oligosaccharides was tested in an established in vitro system for assessing cellular binding and viral infectivity/replication, and also tested in a newly developed, acute RV infection, in situ piglet model. For the in vitro work, crude HMO isolated from pooled HM, neutral HMO (lacto-N-neotetraose, LNnT; 2′-fucosyllactose) and acidic HMO (aHMO, 3′-sialyllactose, 3′-SL; 6′-sialyllactose, 6′-SL) were tested against the porcine OSU strain and human RV Wa strain. The RV Wa strain was not inhibited by any oligosaccharides. However, the RV OSU strain infectivity was dose-dependently inhibited by sialic acid (SA)-containing HMO. 3′-SL and 6′-SL concordantly inhibited 125I-radiolabelled RV cellular binding and infectivity/replication. For the in situ study, a midline laparotomy was performed on 21-d-old formula-fed piglets and six 10 cm loops of ileum were isolated in situ. Briefly, 2 mg/ml of LNnT, aHMO mixture (40 % 6′-SL/10 % 3′-SL/50 % SA) or media with or without the RV OSU strain (1 × 107 focus-forming units) were injected into the loops and maintained for 6 h. The loops treated with HMO treatments+RV had lower RV replication, as assessed by non-structural protein-4 (NSP4) mRNA expression, than RV-treated loops alone. In conclusion, SA-containing HMO inhibited RV infectivity in vitro; however, both neutral HMO and SA with aHMO decreased NSP4 replication during acute RV infection in situ.

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