scholarly journals In Vitro Fermentation of Porcine Milk Oligosaccharides and Galacto-oligosaccharides Using Piglet Fecal Inoculum

2016 ◽  
Vol 64 (10) ◽  
pp. 2127-2133 ◽  
Author(s):  
Elisabetta Difilippo ◽  
Feipeng Pan ◽  
Madelon Logtenberg ◽  
Rianne (H.A.M.) Willems ◽  
Saskia Braber ◽  
...  
Keyword(s):  
Milk Oligosaccharides ◽  
In Vitro Fermentation ◽  
Porcine Milk

scholarly journals In Vitro Fermentation of Breast Milk Oligosaccharides by Bifidobacterium infantis and Lactobacillus gasseri

2006 ◽  
Vol 72 (6) ◽  
pp. 4497-4499 ◽  
Author(s):  
Robert E. Ward ◽  
Milady Ni�onuevo ◽  
David A. Mills ◽  
Carlito B. Lebrilla ◽  
J. Bruce German
Keyword(s):  
Breast Milk ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Human Milk Oligosaccharides ◽  
Bifidobacterium Infantis ◽  
Milk Oligosaccharides ◽  
In Vitro Fermentation ◽  
Bacterial Populations ◽  
Lactobacillus Gasseri

ABSTRACT It has been proposed that human milk oligosaccharides (HMO) function as a prebiotic for bifidobacteria, yet this activity has not been adequately investigated. In this study, Bifidobacterium infantis was shown to ferment purified HMO as a sole carbon source, while another gut commensal, Lactobacillus gasseri, did not ferment HMO. Our results support the hypothesis that HMO selectively amplify bacterial populations in the infant intestine.

scholarly journals In vitro fermentation of human milk oligosaccharides by ascending colonic microbiota from formula‐fed and sow‐reared piglets

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Min Li ◽  
Laura L Bauer ◽  
Xin Chen ◽  
Theresa Kuhlenschmidt ◽  
Mark S Kuhlenschmidt ◽  
...  
Keyword(s):  
Human Milk ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
In Vitro Fermentation ◽  
Colonic Microbiota

scholarly journals Distribution of In Vitro Fermentation Ability of Lacto-N-Biose I, a Major Building Block of Human Milk Oligosaccharides, in Bifidobacterial Strains

2009 ◽  
Vol 76 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Jin-zhong Xiao ◽  
Sachiko Takahashi ◽  
Mamoru Nishimoto ◽  
Toshitaka Odamaki ◽  
Tomoko Yaeshima ◽  
...  
Keyword(s):  
Human Milk ◽  
Building Block ◽  
Bifidobacterium Longum ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
Carbohydrate Source ◽  
In Vitro Fermentation ◽  
Predominant Species ◽  
Almost All

ABSTRACT This study investigated the potential utilization of lacto-N-biose I (LNB) by individual strains of bifidobacteria. LNB is a building block for the human milk oligosaccharides, which have been suggested to be a factor for selective growth of bifidobacteria. A total of 208 strains comprising 10 species and 4 subspecies were analyzed for the presence of the galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) gene (lnpA) and examined for growth when LNB was used as the sole carbohydrate source. While all strains of Bifidobacterium longum subsp. longum, B. longum subsp. infantis, B. breve, and B. bifidum were able to grow on LNB, none of the strains of B. adolescentis, B. catenulatum, B. dentium, B. angulatum, B. animalis subsp. lactis, and B. thermophilum showed any growth. In addition, some strains of B. pseudocatenulatum, B. animalis subsp. animalis, and B. pseudolongum exhibited the ability to utilize LNB. With the exception for B. pseudocatenulatum, the presence of lnpA coincided with LNB utilization in almost all strains. These results indicate that bifidobacterial species, which are the predominant species found in infant intestines, are potential utilizers of LNB. These findings support the hypothesis that GLNBP plays a key role in the colonization of bifidobacteria in the infant intestine.

scholarly journals Preservation of Human Gut Microbiota Inoculums for In Vitro Fermentations Studies

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Nelson Mota de Carvalho ◽  
Diana Luazi Oliveira ◽  
Mayra Anton Dib Saleh ◽  
Manuela Pintado ◽  
Ana Raquel Madureira
Keyword(s):  
Gut Microbiota ◽  
Metabolic Profile ◽  
Bacterial Count ◽  
Glycerol Solution ◽  
Metabolic Profiles ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro Fermentation ◽  
Fecal Samples

The use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at −20 °C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at −20 °C for at least 3 months, without interfering with the normal course of colonic fermentation.

Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.

2021 ◽  
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Hong Yao ◽  
Barbara Williams ◽  
Bernadine Flanagan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Cell Walls ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Plant Cell Walls ◽  
Human Gut ◽  
In Vitro Fermentation

Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the...

Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes

2021 ◽  
Vol 258 ◽  
pp. 117698
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Bernadine M. Flanagan ◽  
Barbara A. Williams ◽  
Michael J. Gidley
Keyword(s):  
In Vitro Fermentation

scholarly journals Effects of bismuth subsalicylate and calcium-ammonium nitrate on ruminal in vitro fermentation of bahiagrass hay with supplemental molasses

animal ◽  
2021 ◽  
Vol 15 (5) ◽  
pp. 100195
Author(s):  
D.D. Henry ◽  
F.M. Ciriaco ◽  
R.C. Araujo ◽  
M.E. Garcia-Ascolani ◽  
P.L.P. Fontes ◽  
...  
Keyword(s):  
Ammonium Nitrate ◽  
In Vitro Fermentation ◽  
Calcium Ammonium Nitrate ◽  
Bismuth Subsalicylate
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