scholarly journals IS26-Mediated Genetic Rearrangements in Salmonella Genomic Island 1 of Proteus mirabilis

2019 ◽  
Vol 10 ◽  
Author(s):  
Xue-Chun Wang ◽  
Chang-Wei Lei ◽  
Zhuang-Zhuang Kang ◽  
Yu Zhang ◽  
Hong-Ning Wang
Keyword(s):  
Proteus Mirabilis ◽  
Genomic Island ◽  
Genetic Rearrangements

scholarly journals Nosocomial Outbreak of Carbapenemase-Producing Proteus mirabilis With Two Novel Salmonella Genomic Island 1 Variants Carrying Different blaNDM–1 Gene Copies in China

2022 ◽  
Vol 12 ◽  
Author(s):  
Lang Yang ◽  
Hong He ◽  
Qichao Chen ◽  
Kaiying Wang ◽  
Yanfeng Lin ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Copy Number ◽  
Genomic Island ◽  
Gene Copy Number ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Gene Copy ◽  
Nosocomial Outbreak ◽  
Gene Copy Number Variation ◽  
Gene Copies

NDM-1-producing multidrug-resistant Proteus mirabilis brings formidable clinical challenges. We report a nosocomial outbreak of carbapenem-resistant P. mirabilis in China. Six P. mirabilis strains collected in the same ward showed close phylogenetic relatedness, indicating clonal expansion. Illumina and MinION sequencing revealed that three isolates harbored a novel Salmonella genomic island 1 carrying a blaNDM–1 gene (SGI1-1NDM), while three other isolates showed elevated carbapenem resistance and carried a similar SGI1 but with two blaNDM–1 gene copies (SGI1-2NDM). Four new single nucleotide mutations were present in the genomes of the two-blaNDM–1-harboring isolates, indicating later emergence of the SGI1-2NDM structure. Passage experiments indicated that both SGI variants were stably persistent in this clone without blaNDM–1 copy number changes. This study characterizes two novel blaNDM–1-harboring SGI1 variants in P. mirabilis and provides a new insight into resistance gene copy number variation in bacteria.

scholarly journals Emergence of Extensively Drug-Resistant Proteus mirabilis Harboring a Conjugative NDM-1 Plasmid and a Novel Salmonella Genomic Island 1 Variant, SGI1-Z

2015 ◽  
Vol 59 (10) ◽  
pp. 6601-6604 ◽  
Author(s):  
Shangshang Qin ◽  
Hui Qi ◽  
Qijing Zhang ◽  
Di Zhao ◽  
Zhen-Zhen Liu ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Molecular Analysis ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Bacterial Species ◽  
Clinical Treatment ◽  
Drug Resistant ◽  
Content Type ◽  
Extensively Drug Resistant

ABSTRACTAcquisition ofblaNDM-1in bacterial species, such asProteus mirabilisthat is intrinsically resistant to tetracycline, tigecycline and colistin, will make clinical treatment extremely difficult. Here, we characterized an NDM-1-producing clinical isolate ofP. mirabilis(PM58) that displayed an extensively drug-resistant (XDR) phenotype, susceptible only to aztreonam. Molecular analysis revealed that PM58 harbored both a conjugative NDM-1 plasmid and a novelSalmonellagenomic island 1 variant on chromosome.

scholarly journals Salmonella Genomic Island 1 (SGI1), Variant SGI1-I, and New Variant SGI1-O in Proteus mirabilis Clinical and Food Isolates from China

2007 ◽  
Vol 52 (1) ◽  
pp. 340-344 ◽  
Author(s):  
David A. Boyd ◽  
Xiaolu Shi ◽  
Qing-hua Hu ◽  
Lai King Ng ◽  
Benoit Doublet ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Genomic Island ◽  
New Variant

ABSTRACT Salmonella genomic island 1 (SGI1) and variants (SGI1-I and the new variant SGI1-O) were mapped in five strains of Proteus mirabilis isolated from humans and food in China. Sequencing showed that SGI1 and variants were integrated at the 3′ end of the chromosomal thdF gene as previously described for Salmonella strains.

scholarly journals Potential integration sites of the Salmonella genomic island 1 in Proteus mirabilis and other bacteria

2007 ◽  
Vol 59 (4) ◽  
pp. 801-803 ◽  
Author(s):  
Benoît Doublet ◽  
George R. Golding ◽  
Michael R. Mulvey ◽  
Axel Cloeckaert
Keyword(s):  
Proteus Mirabilis ◽  
Genomic Island ◽  
Integration Sites ◽  
Potential Integration

Identification of Proteus genomic island 2 variants in two clonal Proteus mirabilis isolates with coexistence of a novel genomic resistance island PmGRI1

2020 ◽  
Vol 75 (9) ◽  
pp. 2503-2507
Author(s):  
Chang-Wei Lei ◽  
Tian-Ge Yao ◽  
Jia Yan ◽  
Bo-Yang Li ◽  
Xue-Chun Wang ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Genomic Islands ◽  
Class 1 Integron ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Resistance Island ◽  
Integrative And Conjugative Element

Abstract Objectives To characterize the MDR genomic islands (GIs) in Proteus mirabilis isolates. Methods Two P. mirabilis strains (C55 and C74) of chicken origin were subjected to WGS (HiSeq and PacBio) and the MDR GIs were determined. Results P. mirabilis strains C55 and C74 are clonal strains and harbour different Proteus genomic island 2 (PGI2) variants (PGI2-C55 and PGI2-C74). The MDR region of PGI2-C55 is composed of two class 1 integrons, separated by a region containing seven copies of IS26 and eight resistance genes, including blaCTX-M-3 and fosA3. The region in PGI2-C74 is a complete In4-type class 1 integron, harbouring five gene cassettes (dfrA16, blaCARB-2, aadA2, cmlA1 and aadA1). In addition, C55 and C74 carry an SXT/R391 integrative and conjugative element (ICEPmiJpn1), harbouring blaCMY-2, and a novel 50.46 kb genomic resistance island named PmGRI1-C55. PmGRI1-C55 harbours a tyrosine-type recombinase/integrase that might be responsible for the integration of PmGRI1-C55 at the 3′ end of tRNA-Sec. It carries an MDR region derived from Tn2670 that harbours a Tn21 region and carries six resistance genes (catA1, blaTEM-1b, aphA1a, sul2, strA and strB). Blast analysis showed diverse PmGRI1 variants in P. mirabilis and Escherichia coli strains. Conclusions The finding of the two new PGI2 variants highlights that the homologous recombination between shared components of class 1 integrons and transposition by IS26 promote the diversity of MDR regions in PGI2. PmGRI1 is a new GI that carries various resistance genes identified in P. mirabilis and E. coli.

scholarly journals Novel Insertion Sequence- and Transposon-Mediated Genetic Rearrangements in Genomic Island SGI1 of Salmonella enterica Serovar Kentucky

2008 ◽  
Vol 52 (10) ◽  
pp. 3745-3754 ◽  
Author(s):  
Benoît Doublet ◽  
Karine Praud ◽  
Sophie Bertrand ◽  
Jean-Marc Collard ◽  
François-Xavier Weill ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Tetracycline Resistance ◽  
Gene Clusters ◽  
Mercury Resistance ◽  
Reading Frame ◽  
Tetracycline Resistance Genes ◽  
Serovar Typhimurium ◽  
Genetic Rearrangements

ABSTRACT Salmonella genomic island 1 (SGI1) is an integrative mobilizable element that harbors a multidrug resistance (MDR) gene cluster. Since its identification in epidemic Salmonella enterica serovar Typhimurium DT104 strains, variant SGI1 MDR gene clusters conferring different MDR phenotypes have been identified in several S. enterica serovars and classified as SGI1-A to -O. A study was undertaken to characterize SGI1 from serovar Kentucky strains isolated from travelers returning from Africa. Several strains tested were found to contain the partially characterized variant SGI1-K, recently described in a serovar Kentucky strain isolated in Australia. This variant contained only one cassette array, aac(3)-Id-aadA7, and an adjacent mercury resistance module. Here, the uncharacterized part of SGI1-K was sequenced. Downstream of the mer module similar to that found in Tn21, a mosaic genetic structure was found, comprising (i) part of Tn1721 containing the tetracycline resistance genes tetR and tet(A); (ii) part of Tn5393 containing the streptomycin resistance genes strAB, IS1133, and a truncated tnpR gene; and (iii) a Tn3-like region containing the tnpR gene and the β-lactamase bla TEM-1 gene flanked by two IS26 elements in opposite orientations. The rightmost IS26 element was shown to be inserted into the S044 open reading frame of the SGI1 backbone. This variant MDR region was named SGI1-K1 according to the previously described variant SGI1-K. Other SGI1-K MDR regions due to different IS26 locations, inversion, and partial deletions were characterized and named SGI1-K2 to -K5. Two new SGI1 variants named SGI1-P1 and -P2 contained only the Tn3-like region comprising the β-lactamase bla TEM-1 gene flanked by the two IS26 elements inserted into the SGI1 backbone. Three other new variants harbored only one IS26 element inserted in place of the MDR region of SGI1 and were named SGI1-Q1 to -Q3. Thus, in serovar Kentucky, the SGI1 MDR region undergoes recombinational and insertional events of transposon and insertion sequences, resulting in a higher diversity of MDR gene clusters than previously reported and consequently a higher diversity of MDR phenotypes.

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