scholarly journals Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

2014 ◽  
Vol 70 (1) ◽  
pp. 98-102 ◽  
Author(s):  
Delphine Girlich ◽  
Laurent Dortet ◽  
Laurent Poirel ◽  
Patrice Nordmann
Keyword(s):  
Clinical Isolate ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Carbapenemase Gene

scholarly journals Emergence of Extensively Drug-Resistant Proteus mirabilis Harboring a Conjugative NDM-1 Plasmid and a Novel Salmonella Genomic Island 1 Variant, SGI1-Z

2015 ◽  
Vol 59 (10) ◽  
pp. 6601-6604 ◽  
Author(s):  
Shangshang Qin ◽  
Hui Qi ◽  
Qijing Zhang ◽  
Di Zhao ◽  
Zhen-Zhen Liu ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Molecular Analysis ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Bacterial Species ◽  
Clinical Treatment ◽  
Drug Resistant ◽  
Content Type ◽  
Extensively Drug Resistant

ABSTRACTAcquisition ofblaNDM-1in bacterial species, such asProteus mirabilisthat is intrinsically resistant to tetracycline, tigecycline and colistin, will make clinical treatment extremely difficult. Here, we characterized an NDM-1-producing clinical isolate ofP. mirabilis(PM58) that displayed an extensively drug-resistant (XDR) phenotype, susceptible only to aztreonam. Molecular analysis revealed that PM58 harbored both a conjugative NDM-1 plasmid and a novelSalmonellagenomic island 1 variant on chromosome.

scholarly journals Chromosomal Amplification of the blaOXA-58 Carbapenemase Gene in a Proteus mirabilis Clinical Isolate

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Delphine Girlich ◽  
Rémy A. Bonnin ◽  
Pierre Bogaerts ◽  
Morgane De Laveleye ◽  
Daniel T. Huang ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Resistance Genes ◽  
Proteus Mirabilis ◽  
Large Subunit ◽  
Antibiotic Resistance Genes ◽  
Biochemical Tests ◽  
Content Type ◽  
Class D ◽  
Carbapenemase Gene ◽  
Human Infections

ABSTRACT Horizontal gene transfer may occur between distantly related bacteria, thus leading to genetic plasticity and in some cases to acquisition of novel resistance traits. Proteus mirabilis is an enterobacterial species responsible for human infections that may express various acquired β-lactam resistance genes, including different classes of carbapenemase genes. Here we report a Proteus mirabilis clinical isolate (strain 1091) displaying resistance to penicillin, including temocillin, together with reduced susceptibility to carbapenems and susceptibility to expanded-spectrum cephalosporins. Using biochemical tests, significant carbapenem hydrolysis was detected in P. mirabilis 1091. Since PCR failed to detect acquired carbapenemase genes commonly found in Enterobacteriaceae, we used a whole-genome sequencing approach that revealed the presence of bla OXA-58 class D carbapenemase gene, so far identified only in Acinetobacter species. This gene was located on a 3.1-kb element coharboring a bla AmpC-like gene. Remarkably, these two genes were bracketed by putative XerC-XerD binding sites and inserted at a XerC-XerD site located between the terminase-like small- and large-subunit genes of a bacteriophage. Increased expression of the two bla genes resulted from a 6-time tandem amplification of the element as revealed by Southern blotting. This is the first isolation of a clinical P. mirabilis strain producing OXA-58, a class D carbapenemase, and the first description of a XerC-XerD-dependent insertion of antibiotic resistance genes within a bacteriophage. This study revealed a new role for the XerC-XerD recombinase in bacteriophage biology.

scholarly journals European clinical isolate of Proteus mirabilis harbouring the Salmonella genomic island 1 variant SGI1-O

2010 ◽  
Vol 65 (10) ◽  
pp. 2260-2262 ◽  
Author(s):  
Benoît Doublet ◽  
Laurent Poirel ◽  
Karine Praud ◽  
Patrice Nordmann ◽  
Axel Cloeckaert
Keyword(s):  
Clinical Isolate ◽  
Proteus Mirabilis ◽  
Genomic Island

scholarly journals Nosocomial Outbreak of Carbapenemase-Producing Proteus mirabilis With Two Novel Salmonella Genomic Island 1 Variants Carrying Different blaNDM–1 Gene Copies in China

2022 ◽  
Vol 12 ◽  
Author(s):  
Lang Yang ◽  
Hong He ◽  
Qichao Chen ◽  
Kaiying Wang ◽  
Yanfeng Lin ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Copy Number ◽  
Genomic Island ◽  
Gene Copy Number ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Gene Copy ◽  
Nosocomial Outbreak ◽  
Gene Copy Number Variation ◽  
Gene Copies

NDM-1-producing multidrug-resistant Proteus mirabilis brings formidable clinical challenges. We report a nosocomial outbreak of carbapenem-resistant P. mirabilis in China. Six P. mirabilis strains collected in the same ward showed close phylogenetic relatedness, indicating clonal expansion. Illumina and MinION sequencing revealed that three isolates harbored a novel Salmonella genomic island 1 carrying a blaNDM–1 gene (SGI1-1NDM), while three other isolates showed elevated carbapenem resistance and carried a similar SGI1 but with two blaNDM–1 gene copies (SGI1-2NDM). Four new single nucleotide mutations were present in the genomes of the two-blaNDM–1-harboring isolates, indicating later emergence of the SGI1-2NDM structure. Passage experiments indicated that both SGI variants were stably persistent in this clone without blaNDM–1 copy number changes. This study characterizes two novel blaNDM–1-harboring SGI1 variants in P. mirabilis and provides a new insight into resistance gene copy number variation in bacteria.

Emergence of Imipenem Resistance in a CpxA-H208P-Variant-Producing Proteus mirabilis Clinical Isolate

2020 ◽  
Author(s):  
Marion Lecuru ◽  
Marie-Hélène Nicolas-Chanoine ◽  
Sébastien Tanaka ◽  
Philippe Montravers ◽  
Laurence Armand-Lefevre ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Proteus Mirabilis ◽  
Imipenem Resistance

scholarly journals New Variant of mcr-3 in an Extensively Drug-Resistant Escherichia coli Clinical Isolate Carrying mcr-1 and blaNDM-5

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Lu Liu ◽  
Yu Feng ◽  
Xiaoxia Zhang ◽  
Alan McNally ◽  
Zhiyong Zong
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Amino Acid Substitutions ◽  
Drug Resistant ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Resistant Genes ◽  
Extensively Drug Resistant ◽  
New Variant ◽  
Carbapenemase Gene

ABSTRACT A colistin- and carbapenem-resistant Escherichia coli clinical isolate was found to carry two plasmid-borne colistin-resistant genes, mcr-1 and the newly identified mcr-3, and a carbapenemase gene, bla NDM-5. mcr-3 is a new variant (mcr-3.5) in the isolate and encodes three amino acid substitutions compared with the original MCR-3. mcr-3 was carried by a TnAs3-like transposon on a self-transmissible IncP plasmid in the isolate, highlighting that mcr-3 may have widely spread.

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