scholarly journals Identification and Characterization of New Resistance-Conferring SGI1s (Salmonella Genomic Island 1) in Proteus mirabilis

2018 ◽  
Vol 9 ◽  
Author(s):  
Luyao Bie ◽  
Meng Fang ◽  
Zhiqiang Li ◽  
Mingyu Wang ◽  
Hai Xu
Keyword(s):  
Proteus Mirabilis ◽  
Genomic Island ◽  
Identification And Characterization

scholarly journals Identification and Characterization of the CRISPR/Cas System in Staphylococcus aureus Strains From Diverse Sources

2021 ◽  
Vol 12 ◽  
Author(s):  
Erick Adrian Cruz-López ◽  
Gildardo Rivera ◽  
María Antonia Cruz-Hernández ◽  
Ana Verónica Martínez-Vázquez ◽  
Graciela Castro-Escarpulli ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Defense Mechanisms ◽  
Genomic Island ◽  
Alternative Therapy ◽  
Bioinformatic Analysis ◽  
Positive Bacterium ◽  
Different Sources ◽  
Identification And Characterization

The CRISPR-Cas [clustered regularly interspaced short palindromic repeats and the CRISPR-associated genes (Cas)] system provides defense mechanisms in bacteria and archaea vs. mobile genetic elements (MGEs), such as plasmids and bacteriophages, which can either be harmful or add sequences that can provide virulence or antibiotic resistance. Staphylococcus aureus is a Gram-positive bacterium that could be the etiological agent of important soft tissue infections that can lead to bacteremia and sepsis. The role of the CRISPR-Cas system in S. aureus is not completely understood since there is a lack of knowledge about it. We analyzed 716 genomes and 1 genomic island from GENOMES-NCBI and ENA-EMBL searching for the CRISPR-Cas systems and their spacer sequences (SSs). Our bioinformatic analysis shows that only 0.83% (6/716) of the analyzed genomes harbored the CRISPR-Cas system, all of them were subtype III-A, which is characterized by the presence of the cas10/csm1 gene. Analysis of SSs showed that 91% (40/44) had no match to annotated MGEs and 9% of SSs corresponded to plasmids and bacteriophages, indicating that those phages had infected those S. aureus strains. Some of those phages have been proposed as an alternative therapy in biofilm-forming or infection with S. aureus strains, but these findings indicate that such antibiotic phage strategy would be ineffective. More research about the CRISPR/Cas system is necessary for a bigger number of S. aureus strains from different sources, so additional features can be studied.

Sign in / Sign up

Export Citation Format

Share Document