scholarly journals Coding Cell Identity of Human Skeletal Muscle Progenitor Cells Using Cell Surface Markers: Current Status and Remaining Challenges for Characterization and Isolation

2019 ◽  
Vol 7 ◽  
Author(s):  
Sin-Ruow Tey ◽  
Samantha Robertson ◽  
Eileen Lynch ◽  
Masatoshi Suzuki
Keyword(s):  
Skeletal Muscle ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Current Status ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Cell Identity ◽  
Muscle Progenitor Cells

Metabolic glycan labeling-assisted discovery of cell-surface markers for primary neural stem and progenitor cells

2018 ◽  
Vol 54 (43) ◽  
pp. 5486-5489
Author(s):  
Qing-Ran Bai ◽  
Lu Dong ◽  
Yi Hao ◽  
Xing Chen ◽  
Qin Shen
Keyword(s):  
Mass Spectrometry ◽  
Stem Cell ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Neural Stem Cell ◽  
Surface Markers ◽  
Metabolic Labeling ◽  
Cell Surface Markers ◽  
Stem And Progenitor Cells

Metabolic labeling with azidosugars in a neural stem cell (NSC)-enriched endothelial coculture followed by mass-spectrometry profiling identifies sialoglycoproteins on NSCs.

Developing Cell-Specific Antibodies to Endothelial Progenitor Cells Using Avian Immune Phage Display Technology

2011 ◽  
Vol 16 (7) ◽  
pp. 744-754 ◽  
Author(s):  
Tyrone Bowes ◽  
Shirley A. Hanley ◽  
Aaron Liew ◽  
Marc Eglon ◽  
Kaveh Mashayekhi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Phage Display ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Endothelial Progenitor ◽  
Phage Display Technology ◽  
Display Technology

This study aims at generating immune chicken phage display libraries and single-chain antibodies (scFvs) specifically directed against cell surface markers of cultured peripheral blood mononuclear cells (PBMCs) that contain endothelial progenitor cells (EPCs). In contrast to previous approaches that use well-defined recombinant antigens attached to plastic surfaces that may alter the structure of the proteins, the authors describe a method that maintains the cell surface markers on live cells while providing the opportunity to rapidly screen entire libraries for antibodies that bind to unknown cell surface markers of progenitor/stem cells. Chickens immunized with live EPCs, consisting of a heterogeneous population of lymphocytes and monocytes, demonstrated a robust immune response. After three rounds of biopanning, the authors purified and characterized three unique scFvs called UG1-3. Codon-optimized recombinant UG1 (gUG-1) shows binding by flow cytometry to circulating CD14-positive cells in peripheral blood consistent with predominant expression of a target protein on monocyte subsets. The authors describe the successful use of immunization of chickens for the generation of scFvs against a heterogenous population of EPCs displaying unknown cell surface markers and demonstrate the strong potential of phage display technology in the development of reagents for the isolation and characterization of stem/progenitor cells.

scholarly journals Retinoic acid maintains human skeletal muscle progenitor cells in an immature state

2016 ◽  
Vol 74 (10) ◽  
pp. 1923-1936 ◽  
Author(s):  
Marina El Haddad ◽  
Cécile Notarnicola ◽  
Brendan Evano ◽  
Nour El Khatib ◽  
Marine Blaquière ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Retinoic Acid ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Muscle Progenitor Cells

scholarly journals Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells

2019 ◽  
Vol 10 ◽  
Author(s):  
Brandon J. Gheller ◽  
Jamie E. Blum ◽  
Edward K. Merritt ◽  
Bethany P. Cummings ◽  
Anna E. Thalacker-Mercer
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
Peptide Yy ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Muscle Progenitor Cells

scholarly journals Isolation and in vitro propagation of human skeletal muscle progenitor cells from fetal muscle

2013 ◽  
Vol 37 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Tohru Hosoyama ◽  
Michael G. Meyer ◽  
Dan Krakora ◽  
Masatoshi Suzuki
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
In Vitro Propagation ◽  
Human Skeletal Muscle ◽  
Muscle Progenitor Cells ◽  
Fetal Muscle

Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin α6 and CD71

2008 ◽  
Vol 367 (2) ◽  
pp. 256-263 ◽  
Author(s):  
Ryuhei Hayashi ◽  
Masayuki Yamato ◽  
Teiko Saito ◽  
Tetsuro Oshima ◽  
Teruo Okano ◽  
...  
Keyword(s):  
Cell Surface ◽  
Progenitor Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Corneal Epithelial

scholarly journals Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle

2016 ◽  
Vol 7 (2) ◽  
pp. 263-278 ◽  
Author(s):  
Akiyoshi Uezumi ◽  
Masashi Nakatani ◽  
Madoka Ikemoto-Uezumi ◽  
Naoki Yamamoto ◽  
Mitsuhiro Morita ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Surface Protein ◽  
Protein Profiling ◽  
Cell Surface Protein

scholarly journals Rotator cuff tear state modulates self-renewal and differentiation capacity of human skeletal muscle progenitor cells

2016 ◽  
Vol 35 (8) ◽  
pp. 1816-1823 ◽  
Author(s):  
Kelsey A. Thomas ◽  
Michael C. Gibbons ◽  
John G. Lane ◽  
Anshuman Singh ◽  
Samuel R. Ward ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rotator Cuff ◽  
Progenitor Cells ◽  
Rotator Cuff Tear ◽  
Human Skeletal Muscle ◽  
Self Renewal ◽  
Differentiation Capacity ◽  
Cuff Tear ◽  
Muscle Progenitor Cells
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