scholarly journals Isolation and in vitro propagation of human skeletal muscle progenitor cells from fetal muscle

2013 ◽  
Vol 37 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Tohru Hosoyama ◽  
Michael G. Meyer ◽  
Dan Krakora ◽  
Masatoshi Suzuki
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
In Vitro Propagation ◽  
Human Skeletal Muscle ◽  
Muscle Progenitor Cells ◽  
Fetal Muscle

scholarly journals Transient and Stable Overexpression of Extracellular Superoxide Dismutase is Positively Associated with the Myogenic Function of Human Skeletal Muscle-Derived Stem/Progenitor Cells

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 817
Author(s):  
Magdalena Nowaczyk ◽  
Agnieszka Malcher ◽  
Agnieszka Zimna ◽  
Wojciech Łabędź ◽  
Łukasz Kubaszewski ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
In Vitro Culture ◽  
Progenitor Cells ◽  
Cell Population ◽  
Human Skeletal Muscle ◽  
Culture Conditions ◽  
Myogenic Cell ◽  
Lower Percentage ◽  
Infarction Heart

In the present study, the genetic modification of human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) was investigated to identify the optimal protocol for myogenic cell preparation for use in post-infarction heart therapy. We used two types of modifications: GFP-transfection (using electroporation) and SOD3 transduction (using a lentiviral vector). SkMDS/PCs were cultured under different in vitro conditions, including standard (21% oxygen) and hypoxic (3% oxygen), the latter of which corresponded to the prevailing conditions in the post-infarction heart. Transfection/transduction efficacy, skeletal myogenic cell marker expression (CD56), cellular senescence, and apoptosis, as well as the expression of antioxidant (SOD1, SOD2, and SOD3), anti-aging (SIRT1 and FOXO), anti-apoptotic (BCL2), and myogenic (MyoD and MyoG) genes, were evaluated. The percentage of GFP-positive SkMDS/PCs was determined as an indicator of the efficacy of transfection, which reached 55%, while transduction showed better efficiency, reaching approximately 85% as estimated by fluorescence microscopy. The CD56-positive SkMDS/PCs were present in approximately 77% of the tested cells after transient transfection and approximately 96% after transduction. Under standard in vitro culture conditions, the ability of the differentiated, transfected SkMDS/PCs to form myotubes was greater than that of the wild type (WT) cell population (p < 0.001), while the cells transduced with the SOD3 gene exhibited an increase in cell fusion under both standard (p < 0.05) and hypoxic conditions (p < 0.001). In transduced SkMDS/PCs, we observed a positive influence of SOD3 overexpression on cell ageing and apoptosis. We observed an increase in the percentage of young cells under standard (p < 0.05) and hypoxic (p < 0.001) in vitro culture conditions, with a notable decrease in the percentage of senescent and advanced senescent cells in the SOD3-overexpressing cell population detected compared to that observed for the untransduced muscle-derived cells. A lower percentage of apoptotic cells was observed for transduced SkMDS/PCs than that for WT cells under hypoxic in vitro culture conditions. In transiently transfected SkMDS/PCs, we observed significantly higher gene expression levels of SOD2 (almost 40-fold) (p < 0.001) and FOXO (p < 0.05) (approximately 3-fold) under both normoxic and hypoxic culture conditions and of BCL2 under hypoxia compared to those observed in untreated cells (WT). In addition, myogenic genes showed a significant increase in MyoD (almost 18-fold) expression under standard culture conditions (p < 0.0001) and decreased MyoG expression (approximately 2-fold) after transfection (p < 0.05) compared with that detected in the WT skeletal muscle-derived cell control. Taken together, these results demonstrate that SOD3-tranduced skeletal muscle-derived cells may have potential for use in the regenerative treatment of the post-infarction heart.

scholarly journals Retinoic acid maintains human skeletal muscle progenitor cells in an immature state

2016 ◽  
Vol 74 (10) ◽  
pp. 1923-1936 ◽  
Author(s):  
Marina El Haddad ◽  
Cécile Notarnicola ◽  
Brendan Evano ◽  
Nour El Khatib ◽  
Marine Blaquière ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Retinoic Acid ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Muscle Progenitor Cells

scholarly journals Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells

2019 ◽  
Vol 10 ◽  
Author(s):  
Brandon J. Gheller ◽  
Jamie E. Blum ◽  
Edward K. Merritt ◽  
Bethany P. Cummings ◽  
Anna E. Thalacker-Mercer
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
Peptide Yy ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Muscle Progenitor Cells

scholarly journals Rotator cuff tear state modulates self-renewal and differentiation capacity of human skeletal muscle progenitor cells

2016 ◽  
Vol 35 (8) ◽  
pp. 1816-1823 ◽  
Author(s):  
Kelsey A. Thomas ◽  
Michael C. Gibbons ◽  
John G. Lane ◽  
Anshuman Singh ◽  
Samuel R. Ward ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rotator Cuff ◽  
Progenitor Cells ◽  
Rotator Cuff Tear ◽  
Human Skeletal Muscle ◽  
Self Renewal ◽  
Differentiation Capacity ◽  
Cuff Tear ◽  
Muscle Progenitor Cells

scholarly journals Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0232081 ◽  
Author(s):  
Anna Urciuolo ◽  
Elena Serena ◽  
Rusha Ghua ◽  
Susi Zatti ◽  
Monica Giomo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Single Fiber ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals An in vitro human skeletal muscle model: coculture of myotubes,neuron-like cells, and the capillary network

2017 ◽  
Vol 41 ◽  
pp. 514-525
Author(s):  
Ayşe Burcu ERTAN ◽  
Halime KENAR ◽  
Tahsin BEYZADEOĞLU ◽  
Fatma Neşe KÖK ◽  
Gamze TORUN KÖSE
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Capillary Network ◽  
Muscle Model

scholarly journals FGF-2–dependent signaling activated in aged human skeletal muscle promotes intramuscular adipogenesis

2021 ◽  
Vol 118 (37) ◽  
pp. e2021013118 ◽  
Author(s):  
Sebastian Mathes ◽  
Alexandra Fahrner ◽  
Umesh Ghoshdastider ◽  
Hannes A. Rüdiger ◽  
Michael Leunig ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Transcriptional Activation ◽  
Human Skeletal Muscle ◽  
Muscle Growth ◽  
Muscle Cells ◽  
Adeno Associated Virus ◽  
Adult Skeletal Muscle

Aged skeletal muscle is markedly affected by fatty muscle infiltration, and strategies to reduce the occurrence of intramuscular adipocytes are urgently needed. Here, we show that fibroblast growth factor-2 (FGF-2) not only stimulates muscle growth but also promotes intramuscular adipogenesis. Using multiple screening assays upstream and downstream of microRNA (miR)-29a signaling, we located the secreted protein and adipogenic inhibitor SPARC to an FGF-2 signaling pathway that is conserved between skeletal muscle cells from mice and humans and that is activated in skeletal muscle of aged mice and humans. FGF-2 induces the miR-29a/SPARC axis through transcriptional activation of FRA-1, which binds and activates an evolutionary conserved AP-1 site element proximal in the miR-29a promoter. Genetic deletions in muscle cells and adeno-associated virus–mediated overexpression of FGF-2 or SPARC in mouse skeletal muscle revealed that this axis regulates differentiation of fibro/adipogenic progenitors in vitro and intramuscular adipose tissue (IMAT) formation in vivo. Skeletal muscle from human donors aged >75 y versus <55 y showed activation of FGF-2–dependent signaling and increased IMAT. Thus, our data highlights a disparate role of FGF-2 in adult skeletal muscle and reveals a pathway to combat fat accumulation in aged human skeletal muscle.

scholarly journals Microdialysis of skeletal muscle at rest

1999 ◽  
Vol 58 (4) ◽  
pp. 919-923 ◽  
Author(s):  
Jan Henriksson
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Human Skeletal Muscle ◽  
Human Metabolism ◽  
High Expectations ◽  
Perfusate Flow ◽  
Muscle Research ◽  
Interstitial Glucose

Techniques in human skeletal muscle research are by necessity predominantly 'descriptive'.Microdialysis has raised high expectations that it could meet the demand for a method that allows 'mechanistic' investigations to be performed in human skeletal muscle. In the present review, some views are given on how well the initial expectations on the use of the microdialysis technique in skeletal muscle have been fulfilled, and the areas in which additional work is needed in order to validate microdialysis as an important metabolic technique in this tissue. The microdialysis catheter has been equated to an artificial blood vessel, which is introduced into the tissue. By means of this 'vessel' the concentrations of compounds in the interstitial space can be monitored. The concentration of substances in the collected samples is dependent on the rate of perfusate flow. When perfusate flow is slow enough to allow complete equilibration between interstitial and perfusate fluids, the concentration in the perfusate is maximal and identical to the interstitial concentration. Microdialysis data may be influenced by changes in blood flow, especially in instances where the tissue diffusivity limits the recovery in vivo, i.e. when recovery in vitro is 100 %, whereas the recovery in vivo is less than 100 %. Microdialysis data indicate that a significant arterial-interstitial glucose concentration gradient exists in skeletal muscle but not in adipose tissue at rest. While the concentrations of glucose and lactate in the dialysate from skeletal muscle are close to the expected values, the glycerol values obtained for muscle are still puzzling. Ethanol added to the perfusate will be cleared by the tissue at a rate that is determined by the nutritive blood flow (the microdialysis ethanol technique). It is concluded that microdialysis of skeletal muscle has become an important technique for mechanistic studies in human metabolism and nutrition.

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