scholarly journals Rotator cuff tear state modulates self-renewal and differentiation capacity of human skeletal muscle progenitor cells

2016 ◽  
Vol 35 (8) ◽  
pp. 1816-1823 ◽  
Author(s):  
Kelsey A. Thomas ◽  
Michael C. Gibbons ◽  
John G. Lane ◽  
Anshuman Singh ◽  
Samuel R. Ward ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rotator Cuff ◽  
Progenitor Cells ◽  
Rotator Cuff Tear ◽  
Human Skeletal Muscle ◽  
Self Renewal ◽  
Differentiation Capacity ◽  
Cuff Tear ◽  
Muscle Progenitor Cells

Retinoic Acid Receptor Agonists Suppress Muscle Fatty Infiltration in Mice

2021 ◽  
Vol 49 (2) ◽  
pp. 332-339
Author(s):  
Hideyuki Shirasawa ◽  
Noboru Matsumura ◽  
Masaki Yoda ◽  
Kazumasa Okubo ◽  
Masayuki Shimoda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Rotator Cuff ◽  
Rotator Cuff Tear ◽  
Gene Expression Analysis ◽  
Retinoic Acid Receptor ◽  
Adipogenic Differentiation ◽  
Fatty Infiltration ◽  
Cuff Tear

Background: The infiltration of fat tissue into skeletal muscle, a condition referred to as muscle fatty infiltration or fatty degeneration, is regarded as an irreversible event that significantly compromises the motor function of skeletal muscle. Purpose: To investigate the effect of retinoic acid receptor (RAR) agonists in suppressing the adipogenic differentiation of fibroadipogenic progenitors (FAPs) in vitro and fatty infiltration after rotator cuff tear in mice. Study Design: Controlled laboratory study. Methods: FAPs isolated from mouse skeletal muscle were cultured in adipogenic differentiation medium in the presence or absence of an RAR agonist. At the end of cell culture, adipogenic differentiation was evaluated by gene expression analysis and oil red O staining. A mouse model of fatty infiltration—which includes the resection of the rotator cuff, removal of the humeral head, and denervation the supraspinatus muscle—was used to induce fatty infiltration in the supraspinatus muscle. The mice were orally or intramuscularly administered with an RAR agonist after the surgery. Muscle fatty infiltration was evaluated by histology and gene expression analysis. Results: RAR agonists effectively inhibited the adipogenic differentiation of FAPs in vitro. Oral and intramuscular administration of RAR agonists suppressed the development of muscle fatty infiltration in the mice after rotator cuff tear. In accordance, we found a significant decrease in the number of intramuscular fat cells and suppressed expression in adipogenic markers. RAR agonists also increased the expression of the transcripts for collagens; however, an accumulation of collagenous tissues was not histologically evident in the present model. Conclusion: Muscle fatty infiltration can be alleviated by RAR agonists through suppressing the adipogenic differentiation of FAPs. The results also suggest that RAR agonists are potential therapeutic agents for treating patients who are at risk of developing muscle fatty infiltration. The consequence of the increased expression of collagen transcripts by RAR agonists needs to be clarified. Clinical Relevance: RAR agonists can be used to prevent the development of muscle fatty infiltration after rotator cuff tear. Nevertheless, further studies are mandatory in a large animal model to examine the safety and efficacy of intramuscular injection of RAR agonists.

scholarly journals Retinoic acid maintains human skeletal muscle progenitor cells in an immature state

2016 ◽  
Vol 74 (10) ◽  
pp. 1923-1936 ◽  
Author(s):  
Marina El Haddad ◽  
Cécile Notarnicola ◽  
Brendan Evano ◽  
Nour El Khatib ◽  
Marine Blaquière ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Retinoic Acid ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Muscle Progenitor Cells

scholarly journals Osteogenic Differentiation Capacity of Human Skeletal Muscle-Derived Progenitor Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56641 ◽  
Author(s):  
Teruyo Oishi ◽  
Akiyoshi Uezumi ◽  
Arihiko Kanaji ◽  
Naoki Yamamoto ◽  
Asami Yamaguchi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Osteogenic Differentiation ◽  
Progenitor Cells ◽  
Human Skeletal Muscle ◽  
Differentiation Capacity

scholarly journals Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells

2019 ◽  
Vol 10 ◽  
Author(s):  
Brandon J. Gheller ◽  
Jamie E. Blum ◽  
Edward K. Merritt ◽  
Bethany P. Cummings ◽  
Anna E. Thalacker-Mercer
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
Peptide Yy ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Muscle Progenitor Cells

scholarly journals Isolation and in vitro propagation of human skeletal muscle progenitor cells from fetal muscle

2013 ◽  
Vol 37 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Tohru Hosoyama ◽  
Michael G. Meyer ◽  
Dan Krakora ◽  
Masatoshi Suzuki
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
In Vitro Propagation ◽  
Human Skeletal Muscle ◽  
Muscle Progenitor Cells ◽  
Fetal Muscle

Accuracy of Rotator Cuff Tears and Tendinosis Diagnoses on Shoulder Ultrasound Performed by a Short-experienced Operator

2018 ◽  
Vol 1 ◽  
pp. 9
Author(s):  
Harshad Arvind Vanjare ◽  
Jyoti Panwar
Keyword(s):  
Rotator Cuff ◽  
Negative Predictive Value ◽  
Rotator Cuff Tear ◽  
Predictive Value ◽  
Rotator Cuff Tears ◽  
Mri Findings ◽  
Cuff Tear ◽  
Experienced Operator ◽  
Magnetic Resonance Imaging Mri ◽  
Sensitivity Specificity

Objective The objective of the study was to assess the accuracy of ultrasound examination for the diagnosis of rotator cuff tear and tendinosis performed by a short experienced operator, compared to magnetic resonance imaging (MRI) results. Method A total of 70 subjects suspected to have rotator cuff tear or tendinosis and planned for shoulder MRI were included in the study. Shoulder ultrasound was performed either before or after the MRI scan on the same day. Ultrasound operator had a short experience in performing an ultrasound of the shoulder. Ultrasound findings were correlated to MRI findings. Results Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for the diagnosis of tendinosis were 58%, 84%, 63%, 80%, and 75%, respectively, and it was 68%, 91%, 73%, 88%, and 85%, respectively, for the diagnosis of rotator cuff tear. Conclusions Sensitivity for diagnosing rotator cuff tear or tendinosis was moderate but had a higher negative predictive value. Thus, the ultrasound operator with a short experience in performing shoulder ultrasound had moderate sensitivity in diagnosing tendinosis or tears; however, could exclude them with confidence.

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