Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

10.3791/58945 ◽  
2019 ◽  
Author(s):  
Qing-Ran Bai ◽  
Lu Dong ◽  
Qin Shen
Keyword(s):  
Cell Surface ◽  
Progenitor Cells ◽  
Surface Markers ◽  
Metabolic Labeling ◽  
Cell Surface Markers ◽  
Stem And Progenitor Cells

Metabolic glycan labeling-assisted discovery of cell-surface markers for primary neural stem and progenitor cells

2018 ◽  
Vol 54 (43) ◽  
pp. 5486-5489
Author(s):  
Qing-Ran Bai ◽  
Lu Dong ◽  
Yi Hao ◽  
Xing Chen ◽  
Qin Shen
Keyword(s):  
Mass Spectrometry ◽  
Stem Cell ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Neural Stem Cell ◽  
Surface Markers ◽  
Metabolic Labeling ◽  
Cell Surface Markers ◽  
Stem And Progenitor Cells

Metabolic labeling with azidosugars in a neural stem cell (NSC)-enriched endothelial coculture followed by mass-spectrometry profiling identifies sialoglycoproteins on NSCs.

Developing Cell-Specific Antibodies to Endothelial Progenitor Cells Using Avian Immune Phage Display Technology

2011 ◽  
Vol 16 (7) ◽  
pp. 744-754 ◽  
Author(s):  
Tyrone Bowes ◽  
Shirley A. Hanley ◽  
Aaron Liew ◽  
Marc Eglon ◽  
Kaveh Mashayekhi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Phage Display ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Endothelial Progenitor ◽  
Phage Display Technology ◽  
Display Technology

This study aims at generating immune chicken phage display libraries and single-chain antibodies (scFvs) specifically directed against cell surface markers of cultured peripheral blood mononuclear cells (PBMCs) that contain endothelial progenitor cells (EPCs). In contrast to previous approaches that use well-defined recombinant antigens attached to plastic surfaces that may alter the structure of the proteins, the authors describe a method that maintains the cell surface markers on live cells while providing the opportunity to rapidly screen entire libraries for antibodies that bind to unknown cell surface markers of progenitor/stem cells. Chickens immunized with live EPCs, consisting of a heterogeneous population of lymphocytes and monocytes, demonstrated a robust immune response. After three rounds of biopanning, the authors purified and characterized three unique scFvs called UG1-3. Codon-optimized recombinant UG1 (gUG-1) shows binding by flow cytometry to circulating CD14-positive cells in peripheral blood consistent with predominant expression of a target protein on monocyte subsets. The authors describe the successful use of immunization of chickens for the generation of scFvs against a heterogenous population of EPCs displaying unknown cell surface markers and demonstrate the strong potential of phage display technology in the development of reagents for the isolation and characterization of stem/progenitor cells.

scholarly journals Single-cell RNA-Sequencing uncovers transcriptional states and fate decisions in haematopoiesis

2017 ◽  
Author(s):  
Emmanouil I. Athanasiadis ◽  
Jan G. Botthof ◽  
Helena Andres ◽  
Lauren Ferreira ◽  
Pietro Lio ◽  
...  
Keyword(s):  
Cell Surface ◽  
Specific Cell ◽  
Surface Markers ◽  
Evolutionary Analysis ◽  
Cell Surface Markers ◽  
Lineage Differentiation ◽  
Lineage Tree ◽  
Stem And Progenitor Cells ◽  
A Cell ◽  
Fate Decision

ABSTRACTThe success of marker-based approaches for dissecting haematopoiesis in mouse and human is reliant on the presence of well-defined cell-surface markers specific for diverse progenitor populations. An inherent problem with this approach is that the presence of specific cell surface markers does not directly reflect the transcriptional state of a cell. Here we used a marker-free approach to computationally reconstruct the blood lineage tree in zebrafish and order cells along their differentiation trajectory, based on their global transcriptional differences. Within the population of transcriptionally similar stem and progenitor cells our analysis revealed considerable cell-to-cell differences in their probability to transition to another, committed state. Once fate decision was executed, the suppression of transcription of ribosomal genes and up-regulation of lineage specific factors coordinately controlled lineage differentiation. Evolutionary analysis further demonstrated that this haematopoietic program was highly conserved between zebrafish and higher vertebrates.

Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin α6 and CD71

2008 ◽  
Vol 367 (2) ◽  
pp. 256-263 ◽  
Author(s):  
Ryuhei Hayashi ◽  
Masayuki Yamato ◽  
Teiko Saito ◽  
Tetsuro Oshima ◽  
Teruo Okano ◽  
...  
Keyword(s):  
Cell Surface ◽  
Progenitor Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Corneal Epithelial
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