scholarly journals The Effect of Enzyme Supplementation on the Apparent Metabolizable Energy and Nutrient Digestibilities of Wheat, Barley, Oats, and Rye for the Young Broiler Chick

1992 ◽  
Vol 71 (10) ◽  
pp. 1710-1721 ◽  
Author(s):  
O.D. FRIESEN ◽  
W. GUENTER ◽  
R.R. MARQUARDT ◽  
B.A. ROTTER
Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 641
Author(s):  
Andrew Dunaway ◽  
Sunday A. Adedokun

This study examined the effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) to a corn-SBM (CS) and a wheat-CS-based (WCS) diet in birds challenged with coccidia vaccine (Coccivac B-52™). The WCS-based diet was produced by replacing 30% of the energy-yielding portions of the CS-based diet with wheat. On day 14, 448 (n = 7) Cobb by-product breeder male broilers were assigned to a 2 (diet types) × 2 (with or without enzyme supplementation) × 2 (0 or 20 × coccidia vaccine challenge; CVC) factorial arrangement of treatments in a completely randomized design for the determination of the apparent metabolizable energy (AME) value of wheat. Treatment effects on jejunum digesta viscosity and AME corrected for nitrogen (AMEn) of the diets were evaluated within each diet type as a 2 × 2 factorial arrangement of treatments, 7- and 14-day post-challenge. Seven-day post-challenge (day 21), dry matter (DM) and energy utilization, AME, and AMEn of the CS- and WCS-based diets decreased (p < 0.05) with CVC. Both AME and AMEn of wheat decreased (p < 0.05) by about a 20% in CVC-birds 7-day post-challenge. Enzyme and CVC resulted in a decrease (p < 0.05) in jejunal digesta viscosity in birds fed the CS-based diets, while there was an interaction (p < 0.05) between CVC and enzyme, with enzyme lowering (p < 0.05) the viscosity of digesta 7-day post-challenge. Results from this study showed that CVC resulted in a 20% decrease in AMEn 7-day post-challenge, while the interaction between exogenous enzyme supplementation and CVC resulted in an improvement in nitrogen utilization (~6%) in CVC birds fed the corn-SBM-based diet 7-days post challenge.


2005 ◽  
Vol 85 (4) ◽  
pp. 493-499 ◽  
Author(s):  
J. L. Rafuse ◽  
F. G. Silversides ◽  
M. R. Bedford ◽  
P. H. Simmins

The feeding value of wheat is affected by the content of soluble non-starch polysaccharides (NSP), which varies among cultivars and the environment in which they are grown. Dietary enzymes can reduce the negative effects of NSP and improve the apparent metabolizable energy (AME) content of the wheat. This study examined differences in feeding value of four wheat cultivars grown in 2 crop years in the Maritime provinces of Canada and the need for enzymes. In a growth trial, diets containing one of the Maritime wheats or corn were fed with or without commercial enzymes containing, for wheat-based diets a mixture of xylanase and protease, and for corn-based diets a mixture of xylanase, protease, and amylase. In a broiler chick bioassay, diets containing 80% wheat were fed with or without a commercial enzyme with xylanase and protease activities. Enzymes significantly lowered body weight gain of birds fed Walton wheat in the second year, and improved the grower feed conversion ratio of birds fed Norboro wheat. Enzyme supplementation significantly improved apparent metabolizable energy and digestibility of crude protein. The AME content did not appear to be a direct indicator of performance. There were significant differences in AME content among the wheat samples. Enzymes generally did not improve performance in this study, possibly due to the relatively low in vitro viscosity values of these samples. Maritime wheats were equal or compared favourably with the sample of wheat from Western Canada and to locally obtained corn. Key words: Wheat, broiler performance, apparent metabolizable energy, enzyme


2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Arturo Cortes ◽  
José Arce-Menocal ◽  
Ernesto Ávila-González ◽  
Carlos López-Coello

Phosphorus (P) bioavailability, crude protein (CP), amino acid (AA) digestibility, and apparent metabolizable energy (AMEn) were determined in broiler chick diets, supplemented with one of two low-oil distiller´s dried grains with solubles (oil contents 6.54% and 5.35% for DDGS A and B, respectively). Diets were provided ad libitum in a mash form, from days 8 to 21 of age. In Experiment 1, one day old male broiler chicks (n=210) were assigned to one of 7 treatments, with 3 replicates of 10 birds each. Animals in treatment 1 (T1) were fed a basal diet formulated to be deficient in nonphytate P (0.14%). Birds in T2 and T3 received the basal diet supplemented with 0.05% or 0.10% of P from monodicalcium phosphate (MCP), respectively. Phosphorus from DDGS was added to diets in T4 (0.05% from DDGS A), T5 (0.1% from DDGS A), T6 (0.05% from DDGS B) and T7 (0.1% from DDGS B). Percent of inclusion of DDGS was dictated by total P content as determined by chemical analyses. Growth performance and P intake were assessed in all birds. Phosphorus bioavailability was determined using the slope ratio assay, with tibia ash as the response variable. For Experiment 2, one-day-old male broiler chicks (n=200), were assigned to 5 treatments with 4 replicates of 10 birds each. A sorghum-soya control diet was given to animals in T1. Treatments 2 and 3 included this same diet supplemented with either 5% or 10% of DDGS A. Birds in T4 and 5 received the control diet with either- 5% or 10% of DDGS B. Amino acid digestibility was measured from ileal contents in 7 euthanized birds from each group at day 21. Apparent metabolizable energy was determined in oven dried feces from all animals during the last 3 days of the experiment.  Weight gain and feed consumption increased (P <0.001) with larger inclusion of dietary P in birds from experiment 1. Relative P bioavailability for DDGS A and B was 72% and 86% respectively, from a P total content of 0.85% and 0.94%.,. Growth performance was similar for all treatment groups in experiment 2 (P >0.05). The average apparent ileal amino acid digestibility coefficient for DDGS A 76.5% and B was 76.4%. No differences were found for essential AA coefficients between the two DDGS (P >0.05). The AMEn values on a dry matter basis were 2828 and 2854 kcal/kg for DDGS A and B, respectively.


1998 ◽  
Vol 78 (4) ◽  
pp. 649-656 ◽  
Author(s):  
T. A. Scott ◽  
F. G. Silversides ◽  
H. L. Classen ◽  
M. L. Swift ◽  
M. R. Bedford

The feeding value ofnine wheat cultivars grown in replicate in three locations in each of two crop years, and 14 barley cultivars grown in seven locations over three crop years was tested with or without an appropriate commercial enzyme using a broiler chick bioassay. Four pens of six male broilers were fed mash diets containing 80% of the test cereal with or without enzyme from 4 to 17 d. Digestibilities were determined using an acid insoluble ash marker at 1.1% of diet. Significant intraclass correlation coefficients between duplicate wheat samples suggested a common effect of the location, but their low to moderate values suggested the presence of random variation or variation due to unique growing conditions within location. For both wheat and barley samples, the growing location affected the feeding value of the cereal grain and subsequent broiler chick performance. Durum wheat cultivars had higher feeding values than that of other wheat classes, both with and without enzyme supplementation, and the response to enzyme supplementation was low, likely reflecting a low level of soluble non-starch polysaccharides. The values of hulless barley cultivars were similar to those of hulled cultivars without enzyme, but the lower crude fibre in hulless barley resulted in higher feeding values when diets were supplemented with enzyme. The response to enzyme supplementation for both wheat and barley cultivars was dependent on the feeding value of the cereal grain without supplementation. Knowledge of variation in enzyme response could be used by feed manufacturers to determine the economic merit of supplementing with enzymes. Key words: Metabolizable energy, broilers, performance, wheat, barley, enzyme


1999 ◽  
Vol 79 (1) ◽  
pp. 59-64 ◽  
Author(s):  
T. A. Scott ◽  
F. G. Silversides ◽  
H. L. Classen ◽  
M. L. Swift ◽  
M. R. Bedford

Samples of wheat (54) and barley (87) were included in a series of broiler chick bioassays. For each cereal, fed at 80% of a basal diet with or without enzyme, apparent metabolizable energy (AME), retained nitrogen, digesta viscosity, excreta dry matter (DM), and broiler performance (feed intake, body weight [BW] gain, and feed conversion) were measured. Correlation coefficients were calculated between the predictor and the performance variables, separated according to whether or not the diets included feed enzymes. Some correlations between measures of AME and BW, feed efficiency, and to a lesser degree feed intake were significant, but none were high. The correlation coefficients were higher when feeds were not supplemented with an enzyme because enzyme supplementation reduced the variation in both AME and performance by reducing the antinutritive effects of non-starch polysaccharides (NSP). Including a measure of voluntary intake in AME (AME level multiplied by feed intake) resulted in higher correlations with performance. Evaluation of the feeding value of wheat and barley by researchers or the feed industry will require the determination of available energy and the NSP contents. Key words: Metabolizable energy, performance, wheat, barley, feed enzymes


2004 ◽  
Vol 84 (3) ◽  
pp. 397-402 ◽  
Author(s):  
J. L. Rafuse ◽  
F. G. Silversides ◽  
M. R. Bedford ◽  
P. H. Simmins

This study examined the effect of four wheat cultivars (Belvedere, Glenlea, Norboro, and Walton) grown in the Maritime provinces of Canada and dietary enzyme supplementation on apparent metabolizable energy (AME), digestibility of crude protein (CP), and performance of laying hens, and compared these values to those obtained using a corn-based diet. Forty groups of three adjacent battery cages, each housing two hens, were fed each diet which included the wheat samples (61.0%) or locally obtained corn (65.1%), with or without commercial enzymes containing a mixture of xylanase, protease, and amylase for corn-based diets and a mixture of xylanase and protease for wheat-based diets. Excreta samples were collected at 17 d for analysis of nutrient retention. The diet that the hens consumed had no significant effect on performance. The feed conversion ratio of hens was lowest (1.79) in the final week of the experiment when egg production was highest (97.0%) and feed intake lowest (109 g bird-1 d-1). The diet significantly affected both AME and digestibility of CP. The wheat cultivar influenced the AME and digestibility of CP when these diets were fed to laying hens, with enzyme supplementation reducing the AME and digestibility of CP for diets containing three (Belvedere, Glenlea, and Walton) of the four wheat cultivars. The significant improvement with enzyme supplementation of the corn-based diet is promising and should be the basis of future studies. Key words: Wheat, layers, apparent metabolizable energy, enzyme


2001 ◽  
Vol 81 (2) ◽  
pp. 237-243 ◽  
Author(s):  
T. A. Scott ◽  
A. B. Pierce

There is a concern that feeding newly harvested cereal grain results in poor performance in poultry. A broiler chick apparent metabolizable energy (AME) bioassay was used to measure changes in feeding value of wheat (n = 14), hulless (n = 10) and hulled (n = 14) barley with or without a commercial enzyme appropriate for wheat- or barley-based diets. In the broiler chick bioassay, four pens of six male broiler chicks were fed ad lib, from 4 to 17 d, diets containing 80% of a test cereal grain and a high-protein basal mixture containing 1% “celite” as an acid-insoluble ash marker. Each of the cereal-grain-based diets, with or without enzyme, was tested within 3 mo of harvest and again 6 mo later. With cereal grain storage there were consistent (P < 0.05) increases in feed intake (FI) between 4 and 17 d and body weight (BW) at 17 d of age with wheat-based diets fed wit h (12.5 and 16.9%, respectively, for FI and BW) or without (16.1 and 22.7%) enzyme. In hulless barley diets without enzyme, there was a significant improvement in BW (7.5%) with storage, while storage had a significant effect on FI and BW (2.9 and 6.3%, respectively) when fed with enzyme. For all other barley diets there was a positive effect of storage on FI and BW, but it was not significant. Feed conversion ratios decreased marginally (P > 0.05) with storage of cereal grain. AME of diets was improved within each period by enzyme supplementation. However, there were measurable decreases (P < 0.05) in AME with storage of cereal grains for the three cereal grains with enzyme supplementation (ranging from –3.2 to –8.2%). The r2 of the above measurements for the different sources of cereal grain between storage periods ranged from 0.65 to 0.94, indicating that the relative ranking of the cereal grains, with respect to feeding value, remained similar during storage. Increases in FI with storage were not significantly related to the attempts of the broiler chicks, to maintain metabolizable energy (ME) intake. Therefore, this increases our concerns that “factors” in newly harvested cereal grains may exist and that they may limit voluntary feed intake and/or growth. Key words: Cereal grain storage, AME, broiler performance, wheat, barley


2021 ◽  
pp. 101288
Author(s):  
M.M. Khalil ◽  
M.R. Abdollahi ◽  
F. Zaefarian ◽  
P.V. Chrystal ◽  
V. Ravindran

Author(s):  
Xingbo Liu ◽  
Kun Xing ◽  
Ran Ning ◽  
Sergi Carné ◽  
Xingqiang Wu ◽  
...  

Abstract Two experiments were conducted to investigate the effects of a combined α-galactosidase and xylanase preparation on nutrients digestibility and growth performance in broiler chickens. Experiment 1 had 240 broilers allocated to 3 treatments with the dietary supplementation of 0, 300 and 500 g/t of the enzyme combination. Diet and amino acid (AA) digestibility were assessed. Experiment 2 was a 2 × 3 (enzyme × diet) factorial arrangement with 10 replicates of 12 male broilers per replicate. Diets were based on corn-Soybean meal (SBM) diet and had 3 nutritional levels (normal, 2% apparent metabolizable energy (AME) and crude protein (CP) reduction, and 4% AME and CP reduction). Each of these diets was fed with or without enzyme supplementation. Growth performance, chyme viscosity, nutrients digestibility, and endogenous enzymes activity were assessed. In experiment 1, enzyme supplementation improved the digestibility of Ca (P = 0.025) and ileal digestibility of total AA, Pro, Alu, Ile, Lys, His, Thr, Glu, Val, Leu, Tyr and Phe (P &lt; 0.05), and also tended to increase the AME of diets (P &lt; 0.10). In experiment 2, broilers fed the corn-SBM diet with 4% nutrient reduction had better growth performance (P &lt; 0.05), jejunal digesta viscosity at 42 days (P &lt; 0.01), and lower digestibility of gross energy (GE) (P &lt; 0.05) when compared to those fed the normal nutrient diet. Enzyme inclusion increased digestibility of CP (P = 0.044), GE (P = 0.009), raffinose (P &lt; 0.001) and stachyose (P &lt; 0.001), improved average daily gain (P = 0.031), and reduced jejunal digesta viscosity at 42 days (P = 0.011). Besides, similar improvements trend in amylase, trypsin, sucrase, and maltase activity with enzyme inclusion were observed as with energy. These data support that the enzyme supplementation increased nutrients and ileal amino acid digestibility, improved performance and endogenous enzymes activity.


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