scholarly journals Coccidia Vaccine Challenge and Exogenous Enzyme Supplementation in Broiler Chicken 1. Effect on Digesta Viscosity, Diet Energy Utilization, and Apparent Metabolizable Energy Value of Wheat

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 641
Author(s):  
Andrew Dunaway ◽  
Sunday A. Adedokun
Keyword(s):  
Metabolizable Energy ◽  
Energy Utilization ◽  
Post Challenge ◽  
Exogenous Enzyme ◽  
Randomized Design ◽  
Apparent Metabolizable Energy ◽  
Enzyme Supplementation ◽  
Digesta Viscosity ◽  
Diet Type

This study examined the effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) to a corn-SBM (CS) and a wheat-CS-based (WCS) diet in birds challenged with coccidia vaccine (Coccivac B-52™). The WCS-based diet was produced by replacing 30% of the energy-yielding portions of the CS-based diet with wheat. On day 14, 448 (n = 7) Cobb by-product breeder male broilers were assigned to a 2 (diet types) × 2 (with or without enzyme supplementation) × 2 (0 or 20 × coccidia vaccine challenge; CVC) factorial arrangement of treatments in a completely randomized design for the determination of the apparent metabolizable energy (AME) value of wheat. Treatment effects on jejunum digesta viscosity and AME corrected for nitrogen (AMEn) of the diets were evaluated within each diet type as a 2 × 2 factorial arrangement of treatments, 7- and 14-day post-challenge. Seven-day post-challenge (day 21), dry matter (DM) and energy utilization, AME, and AMEn of the CS- and WCS-based diets decreased (p < 0.05) with CVC. Both AME and AMEn of wheat decreased (p < 0.05) by about a 20% in CVC-birds 7-day post-challenge. Enzyme and CVC resulted in a decrease (p < 0.05) in jejunal digesta viscosity in birds fed the CS-based diets, while there was an interaction (p < 0.05) between CVC and enzyme, with enzyme lowering (p < 0.05) the viscosity of digesta 7-day post-challenge. Results from this study showed that CVC resulted in a 20% decrease in AMEn 7-day post-challenge, while the interaction between exogenous enzyme supplementation and CVC resulted in an improvement in nitrogen utilization (~6%) in CVC birds fed the corn-SBM-based diet 7-days post challenge.

scholarly journals Protein and Energy Utilization of Black Soldier Fly Maggot at Different age on Chicken

2020 ◽  
Vol 8 (1) ◽  
pp. 52-58
Author(s):  
Nicolas Gomgom Tua Marbun
Keyword(s):  
Research Result ◽  
Nitrogen Retention ◽  
Protein Digestibility ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Study Program ◽  
Black Soldier Fly ◽  
Randomized Design ◽  
Apparent Metabolizable Energy ◽  
Protein And Energy Utilization

This study aims to determine the efficient use of protein and energy of Black Soldier fly larvae at different age levels on chicken. This research was conducted at Jl. RK, Desa Namo Bitang, Kecamatan Pancur Batu Kabupaten Deli Serdang, North Sumatra. Animal Husbandary Study Program, Faculty of Agriculture, Universitas Sumatra Utara, Medan. The design of this study was a completely randomized design (CRD) with 4 treatments and 5 replications. The treatment consisted of P0, P1, P2, and P3 (larvae 14, 21, 28, and 35 days old). Observed variables were protein digestibility, nitrogen retention and apparent metabolizable energy.The results showed that the treatment had a significant effect (P<0,01) on protein digestibility, nitrogen retention and apparent metabolizable energy. Based on research result it can be concluded that maggot of Black soldier fly more efficient at the age between 24-25 days.

scholarly journals Effects of diet type on nutrient utilization and energy balance in drylot heifers1

2020 ◽  
Vol 98 (1) ◽  
Author(s):  
Jessica R Baber ◽  
Tryon A Wickersham ◽  
Jason E Sawyer ◽  
Harvey C Freetly ◽  
Tami M Brown-Brandl ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Nutrient Utilization ◽  
Corn Silage ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Dicalcium Phosphate ◽  
Maternal Tissue ◽  
Randomized Design ◽  
Plastic Bag ◽  
Diet Type

Abstract Feeding cattle in intensified settings allows cow-calf producers to decrease their reliance on grazed forage and utilize alternative feedstuffs. During times of intense management, diet type may alter energy utilization. Fourteen pregnant MARC III heifers (405 ± 44 kg BW) were used in a 180 d experiment to determine effects of diet type on nutrient and energy utilization. Heifers were randomly assigned to one of two treatments, a forage diet (FOR; 2.10 Mcal metabolizable energy [ME]/kg; 95.75% forage) or a concentrate diet (CONC; 2.94 Mcal ME/kg; 71% concentrate), and individually fed to meet maintenance energy requirements (0.135 Mcal ME/kg BW0.75). The CONC diet contained dry-rolled corn, corn stalks (10.16 cm grind size), soybean meal, corn silage (approximately 45% corn grain; stored in a plastic bag), dicalcium phosphate, urea, and a premix pellet; FOR contained alfalfa hay (harvested at mid-bloom), corn silage, dicalcium phosphate, and a premix pellet. Measurements of energy intake and digestibility were measured over a 4-d period on days 116, 172, and 235 of gestation. Using portable headbox calorimeters, measurements of O2, CO2, and CH4 gases were collected over a period of 24 h. Data were analyzed in a completely randomized design with diet as fixed effect. Dry matter and organic matter digestibility were greater for CONC than FOR (P &lt; 0.01). Intake of gross energy (GE) and digestible energy (DE) were greater for FOR (P &lt; 0.01), but by design, ME intake was not different between treatments (P = 0.26). Energy lost as methane (% of GE intake) was not different between treatments (P = 0.49). The ratio of ME to DE was greater for CONC (86.8 vs. 82.8; P = 0.01) than FOR. Heat production relative to ME was not different between treatments (P = 0.85). Maternal tissue energy did not differ and was 1.2 Mcal/d for CONC and 0.9 Mcal/d for FOR (P = 0.73). Greater nitrogen (N) consumption was observed for FOR (192.2 g/d) than CONC (134.0 g/d; P &lt; 0.01), and retained N was greater for FOR than CONC (P &lt; 0.01) on days 116 and 235 of gestation. Neither concentrate-based or forage-based diets affected body condition score (P = 0.26). Heifers fed concentrate-based diets retained more energy in part because they had larger calves, but this energy was not recovered in maternal tissue.

Impact of combined α-galactosidase and xylanase enzymes on growth performance, nutrients digestibility, chyme viscosity, and enzymes activity of broilers fed corn-soybean diets

2021 ◽  
Author(s):  
Xingbo Liu ◽  
Kun Xing ◽  
Ran Ning ◽  
Sergi Carné ◽  
Xingqiang Wu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Average Daily Gain ◽  
Metabolizable Energy ◽  
Enzymes Activity ◽  
Enzyme Supplementation ◽  
Digesta Viscosity ◽  
Nutrients Digestibility ◽  
Endogenous Enzymes

Abstract Two experiments were conducted to investigate the effects of a combined α-galactosidase and xylanase preparation on nutrients digestibility and growth performance in broiler chickens. Experiment 1 had 240 broilers allocated to 3 treatments with the dietary supplementation of 0, 300 and 500 g/t of the enzyme combination. Diet and amino acid (AA) digestibility were assessed. Experiment 2 was a 2 × 3 (enzyme × diet) factorial arrangement with 10 replicates of 12 male broilers per replicate. Diets were based on corn-Soybean meal (SBM) diet and had 3 nutritional levels (normal, 2% apparent metabolizable energy (AME) and crude protein (CP) reduction, and 4% AME and CP reduction). Each of these diets was fed with or without enzyme supplementation. Growth performance, chyme viscosity, nutrients digestibility, and endogenous enzymes activity were assessed. In experiment 1, enzyme supplementation improved the digestibility of Ca (P = 0.025) and ileal digestibility of total AA, Pro, Alu, Ile, Lys, His, Thr, Glu, Val, Leu, Tyr and Phe (P &lt; 0.05), and also tended to increase the AME of diets (P &lt; 0.10). In experiment 2, broilers fed the corn-SBM diet with 4% nutrient reduction had better growth performance (P &lt; 0.05), jejunal digesta viscosity at 42 days (P &lt; 0.01), and lower digestibility of gross energy (GE) (P &lt; 0.05) when compared to those fed the normal nutrient diet. Enzyme inclusion increased digestibility of CP (P = 0.044), GE (P = 0.009), raffinose (P &lt; 0.001) and stachyose (P &lt; 0.001), improved average daily gain (P = 0.031), and reduced jejunal digesta viscosity at 42 days (P = 0.011). Besides, similar improvements trend in amylase, trypsin, sucrase, and maltase activity with enzyme inclusion were observed as with energy. These data support that the enzyme supplementation increased nutrients and ileal amino acid digestibility, improved performance and endogenous enzymes activity.

scholarly journals Insights Into the Proteomic Profile of Newly Harvested Corn and Metagenomic Analysis of the Broiler Intestinal Microbiota

2021 ◽  
Author(s):  
Dafei Yin ◽  
Youli Wang ◽  
Liqun Wang ◽  
Yuqin Wu ◽  
Xiaoyi Bian ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Soluble Sugars ◽  
Pasting Properties ◽  
Metabolizable Energy ◽  
Intestinal Morphology ◽  
Energy Utilization ◽  
Proteomic Profile ◽  
Gut Health ◽  
Digesta Viscosity

Abstract BackgroundThe use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as “new season grain problem”. The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens. MethodsNewly harvested corn stored for either half a month (HM) or two months (TM) was used, and the pasting properties, total soluble sugars, and proteomic analysis technology was used to explore the influence of storage on natural aging corn properties. Additionally, seventy-two 7d Ross 308 male broiler chicken were fed with different stored corn. Apparent metabolizable energy (AME), digesta viscosity, intestinal morphology and microbiota were examined to explore the influence of feed corn storage on broiler chickens. ResultsPasting properties in the TM corn exhibited decreased viscoelastic properties. Proteomic studies found a total of 26 proteins that were differentially expressed between the two treatment groups. Proteins involved in starch and polysaccharides biosynthesis were upregulated in TM compared with HM. Chickens fed on TM diet had higher relative energy utilization compared to the HM birds. With increased corn storage, the relative digesta viscosity decreased significantly (P≤0.05). The total number of goblet cells and lymphocytes was lower in chickens fed the TM diet. The microbiota data showed that the TM chickens had decreased abundance of diarrheal bacteria such as Hungatella hathewayi and Bacteroides fragilis, and increased butyrate-producing bacteria such as Alistipes compared to the HM chickens. ConclusionsStorage of newly harvested corn induced the synthetic reaction of large molecules and changed the solubility of starch and protein with increasing soluble sugars and decreasing pasting properties that may improve the fermentation of intestinal microbiota, improve the energy utilization and protect gut health without the risk of diarrhea.

scholarly journals Metabolizable Energy of Soybean Meal and Canola Meal as Influenced by the Reference Diet Used and Assay Method

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2132
Author(s):  
Shravani Veluri ◽  
Oluyinka Abiona Olukosi
Keyword(s):  
Soybean Meal ◽  
Regression Method ◽  
Metabolizable Energy ◽  
Assay Method ◽  
Canola Meal ◽  
Test Protein ◽  
Apparent Metabolizable Energy ◽  
Inclusion Level ◽  
The Difference ◽  
Diet Type

A 21-day experiment was conducted to study the effect of reference diet type and assay method on apparent metabolizable energy (AME) and nitrogen-corrected (AMEn) of soybean meal (SBM) and canola meal (CM). Broilers (n = 240) were allocated to 10 treatments with eight replicates/treatment and three birds/replicate. Treatments included corn-SBM or corn-CM reference diets (RD). To each RD, 300 or 450 g/kg of SBM or CM were added to make a total of eight test diets. For the difference method, AME of SBM and CM substituted at 300 g/kg in corn-CM RD gave greater AME values compared to inclusion in the corn-SBM RD. The AMEn of SBM increased with increase in inclusion level in the corn-CM RD but AMEn of CM decreased with increased inclusion level of CM in the corn-SBM RD. For the regression method, AME and AMEn of the test feedstuffs were greater with corn-CM RD compared with corn-SBM RD. The AME of SBM was not affected by assay method, whereas AME of CM was lower when determined using the regression method. In conclusion, both the reference diet type and assay method influenced assayed AME and AMEn value of test protein feedstuffs and should be considered in cross-studies comparisons.

Impact of exogenous carbohydrases and phytase on growth performance and nutrient digestibility in broilers

2013 ◽  
Vol 93 (2) ◽  
pp. 243-249 ◽  
Author(s):  
H. Lu ◽  
S.A. Adedokun ◽  
A. Preynat ◽  
V. Legrand-Defretin ◽  
P. A. Geraert ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Metabolizable Energy ◽  
Feed Ratio ◽  
Exogenous Enzyme ◽  
Enzyme Supplementation

Lu, H., Adedokun, S. A., Preynat, A., Legrand-Defretin, V., Geraert, P. A., Adeola, O. and Ajuwon, K. M. 2013. Impact of exogenous carbohydrases and phytase on growth performance and nutrient digestibility in broilers. Can. J. Anim. Sci. 93: 243–249. The effect of supplementing corn–soybean-based diets with Rovabio®Max AP, an exogenous enzyme mix with carbohydrase (xylanase and β-glucanase) and phytase activities to broiler chickens was evaluated. Male Ross 708 broilers were assigned to four diets: positive control (PC), negative control (NC), deficient in metabolizable energy (ME), crude protein (CP), calcium (Ca) and non-phytate phosphorus (nPP), relative to the PC, by 100 kcal kg−1, 5 g kg−1, 1.1 g kg−1and 1.6 g kg−1, respectively, in the starter and grower diets; and NC+Rovabio®at 0.05 or 0.075 g kg−1diet. Starter diets were fed from day 0 to day 21 and grower diets from day 22 to day 42. Body weight (BW) and body weight gain on days 22 and 42 were increased (P<0.05) by enzyme supplementation. Enzyme addition increased (P<0.05) feed intake from day 22 to day 42 and overall (day 0 to day 42). Gain to feed ratio (G:F) was significantly increased (P<0.05) with enzyme supplementation during both starter and grower phases. Enzyme addition increased (P<0.05) serum phosphorus (P) concentration on day 21. On day 42 enzyme supplementation increased (P<0.05) the apparent ileal digestibility of P, dry matter (DM), energy, and N. Addition of a combination of carbohydrases (xylanase and β-glucanase) and phytase to corn–soybean-based diets deficient in ME, CP, Ca and P resulted in a significant increase in growth performance and utilization of P, DM, energy and N in broiler chickens.

scholarly journals Purified glycerin in balanced diets for chicken broilers from 8 to 21 days old

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Mônica Calixto da Silva ◽  
Roberta Gomes Marçal Vieira Vaz ◽  
Kênia Ferreira Rodrigues ◽  
Luciano Fernanades Sousa ◽  
José Henrique Stringhini ◽  
...  
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Control Diet ◽  
Metabolizable Energy ◽  
Feed Conversion ◽  
Gross Margin ◽  
Randomized Design ◽  
Final Weight ◽  
Apparent Metabolizable Energy ◽  
Completely Randomized Design

This paper aimed to evaluate the metabolizability, performance and economic viability of purified glycerin inclusion in balanced diets fed to chicken broilers from 8 to 21 days old. Two experiments were conducted. In the first experiment, 100 broilers (14 days old) were distributed in a completely randomized design into two treatments, with five replications of 10 broilers. Treatments consisted of a control diet and a test diet, in which purified glycerin replaced 10% of the control diet. In the second experiment, 200 broilers (8 days old) were distributed in a completely randomized design into four treatments (0, 2, 4 and 6% of purified glycerin inclusion), with five replications of 10 broilers. The weight gain, feed intake, feed conversion, final weight, apparent metabolizable energy (AME), nitrogen-corrected apparent metabolizable energy (AMEn), metabolizability coefficients of dry matter (DMMC), crude protein (CPMC) and gross energy (GEMC), and the cost of feed per kg of broiler produced were evaluated. The AME, AMEn, DMMC, CPMC and GEMC from the purified glycerin were 3790 and 3560 kcal/kg, and 83.72, 71.52 and 86.27%, respectively. The glycerin levels did not affect (p>0.05) any of the performance characteristics (weight gain, feed intake, feed conversion and final weight). The lowest feeding cost and the highest gross margin were obtained for broilers fed with 6% purified glycerin. The inclusion of 6% purified glycerin in balanced diets for broilers from 8 to 21 days old was technically and economically feasible.

scholarly journals Determination of metabolizable energy value of corn with different average geometric diameters for european quails (Coturnix coturnix coturnix)

2009 ◽  
Vol 52 (4) ◽  
pp. 981-984 ◽  
Author(s):  
Marcelo José de Mello Rezende ◽  
Alessandro Figueiredo Torres ◽  
Luci Sayori Murata ◽  
José Américo Soares Garcia ◽  
Concepta Margaret McManus
Keyword(s):  
Live Weight ◽  
Metabolizable Energy ◽  
Energy Value ◽  
Coturnix Coturnix ◽  
Apparent Metabolizable Energy

Metabolizable energy (ME) of corn with different geometric diameters was determined in European quail with 26 days of age with 124 g of mean live weight, using the Total Collection of Excreta Method. One hundred and twenty five quails were divided in five treatments, five replications with five quails each, with one treatment used to determine endogenous losses. Values of Average Geometric Diameter (AGD) of the corn were 600, 800, 1000 and 1200 µm. ME of corn was not affected by AGD. Average values were 3079, 3274, 3300, 3137 Kcal/kg respectively for apparent metabolizable energy, corrected apparent metabolizable energy, true metabolizable energy, corrected true metabolizable energy.

Enzyme complex addition in barley or rye broiler diets with two energy levels fed from 1 to 21 days

2021 ◽  
pp. 1-11
Author(s):  
Caio Henrique Pereira de Souza ◽  
Márcia Izumi Sakamoto ◽  
Paulo Cesar Pozza ◽  
Tatiana Carlesso dos Santos ◽  
Humberto Marques Lipori ◽  
...  
Keyword(s):  
Energy Levels ◽  
Blood Parameters ◽  
Nutrient Digestibility ◽  
Metabolizable Energy ◽  
Control Treatment ◽  
Enzyme Complex ◽  
Feed Conversion ◽  
Randomized Design ◽  
Apparent Metabolizable Energy ◽  
Completely Randomized Design

Two experiments were conducted to evaluate diet digestibility, performance, digestive parameters, and blood parameters when an enzyme complex (EC) was used in barley- and rye-based diets with different energy levels. In the digestibility assay (exp. I), 108 seventeen-day-old Cobb male broilers were distributed in a completely randomized design in 2 × 2 × 2 + 1 factorial arrangement with two feeds (barley or rye), two EC levels (0% and 0.02%), and two energy levels [3025 and 3125 kcal apparent metabolizable energy (AME)·kg−1], plus a control treatment. In exp. II, 1080 one-day-old Cobb male broilers were distributed in a completely randomized design in 2 × 2 × 2 + 1 factorial arrangement with two feeds (barley or rye), two EC levels (0% and 0.02%), and two energy levels (2875 and 2975 kcal AME·kg−1). No interactions were observed for any variables (exp. I and II). Enzyme complex improved the apparent metabolizable coefficient of gross energy (P = 0.0432) of diets. The EC provided greater weight gain (P = 0.0003) and better feed conversion (P = 0.0025). Intestinal viscosity at 21 d was reduced (P < 0.0001) with the addition of the EC. The EC improved nutrient digestibility and performance, but the effects of energy reduction on performance could not be overcome.

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