scholarly journals Research Note: Antioxidant Activity of Japanese Quail Liver Cytosol in the Absence and Presence of Reduced Glutathione

1992 ◽  
Vol 71 (5) ◽  
pp. 928-931 ◽  
Author(s):  
M.E. SPURLOCK ◽  
J.E. SAVAGE
Keyword(s):  
Antioxidant Activity ◽  
Japanese Quail ◽  
Reduced Glutathione ◽  
Research Note ◽  
Liver Cytosol

scholarly journals Research Note: Eggshell Quality in Japanese Quail Fed Different Fatty Acids

1992 ◽  
Vol 71 (9) ◽  
pp. 1568-1571
Author(s):  
C. VILCHEZ ◽  
S.P. TOUCHBURN ◽  
E.R. CHAVEZ ◽  
P.C. LAGUE
Keyword(s):  
Fatty Acids ◽  
Japanese Quail ◽  
Research Note ◽  
Eggshell Quality

Antioxidant Activity of Peptides Extracted from Brewers’ Spent Grain Peptides

2012 ◽  
Vol 554-556 ◽  
pp. 891-899
Author(s):  
Xu Yan Zong ◽  
Li Li ◽  
Xu Qiao Feng ◽  
Hui Bo Luo ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritive Value ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Protein Hydrolysate ◽  
Low Cost ◽  
Reducing Power ◽  
Gel Permeation Chromatography ◽  
Cosmetic Products ◽  
Spent Grain

Brewer’s spent grain (BSG) protein extracted from BSG was hydrolyzed using Alcalase to produce BSG protein hydrolysate. BSG protein hydrolysate was fractionated by ultrafiltration to obtain brown color BSG peptides. Antioxidant activity of BSG peptides was analyzeded and compared with reduced glutathione (GSH). BSG peptides exhibited 50% of scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical with concentrations less than 0.8 mg/mL, 0.6 mg/mL and 0.6 mg/mL, respectively. The reducing power of BSG peptides was 0.70 at the concentration of 2.00 mg/mL. 86.30% of the total amount of the BSG peptides purified by gel permeation chromatography was below 2000 Da. Because of its antioxidant activity, stability, nutritive value and low cost, BSG peptides exerts a possibility to use in food or cosmetic products.

scholarly journals Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

2015 ◽  
Vol 62 (2) ◽  
pp. 13-19
Author(s):  
Urmila Jarouliya ◽  
Anish Zacharia ◽  
Raj K. Keservani ◽  
Godavarthi B.K.S Prasad
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

scholarly journals The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6005
Author(s):  
Jolanta Flieger ◽  
Michał Flieger
Keyword(s):  
Antioxidant Activity ◽  
Reduced Glutathione ◽  
Array Detector ◽  
Dpph Radical ◽  
Diode Array Detector ◽  
Reverse Phase ◽  
Fast Kinetics ◽  
Ultrasonic Bath ◽  
Rp Hplc ◽  
Scavenging Capacity

The 2,2-diphenyl-1-picrylhydrazyl (DPPH)-reverse phase (RP)-HPLC-diode array detector (DAD) method was tested on standard antioxidants (AOs), i.e., reduced glutathione (GSH), ascorbic acid (vitamin C), and alcoholic extracts of A. podagraria L. An elaborated HPLC procedure enabled the simultaneous measurement of the redox couple DPPH-R (2,2-diphenyl-1-picrylhydrazyl radical)/DPPH-H (2,2-diphenyl-1-picrylhydrazine). Both forms were fully separated (Rs = 2.30, α = 1.65) on a Zorbax Eclipse XDB-C18 column eluted with methanol–water (80:20, v/v) and detected at different wavelengths in the range of 200–600 nm. The absorbance increases of the DPPH-H as well as the DPPH-R peak inhibition were measured at different wavelengths in visible and UV ranges. The chromatographic method was optimized, according to reaction time (slow, fast kinetics), the linearity range of DPPH radical depending on the detection conditions as well as the kind of the investigated antioxidants (reference chemicals and the ground elder prepared from fresh and dry plants). The scavenging capacity was expressed by the use of percentage of peak inhibition and the IC50 parameters. The evaluated extracts displayed antioxidant activity, higher than 20% inhibition against 350 µM DPPH free radical. The results show that extract prepared from dry plants in the ultrasonic bath exhibits the highest antioxidant potential (IC50 = 64.74 ± 0.22 µL/mL).

scholarly journals Hibiscus sabdariffaAffects Ammonium Chloride-Induced Hyperammonemic Rats

2007 ◽  
Vol 4 (3) ◽  
pp. 321-325 ◽  
Author(s):  
M. Mohamed Essa ◽  
P. Subramanian
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ammonium Chloride ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Alcoholic Extract ◽  
Hibiscus Sabdariffa ◽  
Protein Nitrogen ◽  
Antioxidant Effects ◽  
Brain Tissues

Hibiscus sabdariffa(HS) is an edible medicinal plant, indigenous to India, China and Thailand and is used in Ayurveda and traditional medicine. Alcoholic extract of HS leaves (HSEt) was studied for its anti-hyperammonemic and antioxidant effects in brain tissues of ammonium chloride-induced hyperammonemic rats. Oral administration of HSEt (250 mg kg−1body weight) significantly normalizes the levels of ammonia, urea, uric acid, creatinine and non-protein nitrogen in the blood. HSEt significantly reduced brain levels of lipid peroxidation products such as thiobarbituric acid and reactive substances (TBARS) and hydroperoxides (HP). However, the administered extract significantly increased the levels of antioxidants such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) in brain tissues of hyperammonemic rats. This investigation demonstrates significant anti-hyperammonemic and antioxidant activity of HS.

scholarly journals Research Note: Lack of an Effect of High Levels of Menadione on Tumor Development in Japanese Quail Females

1991 ◽  
Vol 70 (11) ◽  
pp. 2382-2385 ◽  
Author(s):  
KARL E. NESTOR ◽  
DEREK A. EMMERSON ◽  
NICHOLAS B. ANTHONY ◽  
KARL E. NESTOR
Keyword(s):  
Japanese Quail ◽  
Tumor Development ◽  
Research Note

Antioxidant activity by reduced glutathione (GSH) assay: in vitro protocol v1 (protocols.io.btaynifw)

protocols.io ◽  
2021 ◽  
Author(s):  
Adrieli Sachett ◽  
Matheus Gallas-Lopes ◽  
Greicy M ◽  
Ana P ◽  
Angelo Piato
Keyword(s):  
Antioxidant Activity ◽  
Reduced Glutathione

scholarly journals NOVEL UMBELLIFERONE PHYTOSOMES: DEVELOPMENT AND OPTIMIZATION USING EXPERIMENTAL DESIGN APPROACH AND EVALUATION OF PHOTO-PROTECTIVE AND ANTIOXIDANT ACTIVITY

2016 ◽  
Vol 9 (1) ◽  
pp. 218 ◽  
Author(s):  
Parul A. Ittadwar ◽  
Prashant K. Puranik
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Enzymes ◽  
Partition Coefficient ◽  
Ex Vivo ◽  
Reduced Glutathione ◽  
Pharmacological Action ◽  
Box Behnken Design ◽  
Coefficient Method ◽  
Better Than

<p><strong>Objective: </strong>The objective of this study was to develop a novel formulation (Phytosome) of umbelliferone with phospholipid for improved permeability, solubility and hence better pharmacological action.</p><p><strong>Methods: </strong>The phytosomal complex was prepared by using solvent evaporation method and optimised by applying the Box-Behnken design on the basis of complexation rate and partition coefficient. The formation of phytosomes was confirmed by FTIR, DSC, SEM, XRD, HPTLC and NMR by comparing the results of the complex with the drug. The photoprotective potential of complex against UV-exposure was evaluated in rats and compared with the drug by incorporating it in a gel and estimating the antioxidant enzymes in skin namely reduced glutathione, superoxide dismutase, lipid peroxidation and catalase.</p><p><strong>Results: </strong>The crystalline drug was completely converted to amorphous complex. The complex showed a good practical yield, drug content and particle size was in the range. The solubility of the complex was determined by partition coefficient method and was found to be better than the drug. The <em>ex vivo</em> and <em>in-vitro</em> permeation of the complex showed improved permeation for complex than the drug. The <em>in vitro</em> antioxidant activity of complex was evaluated by DPPH and ferrozine antioxidant assay and was better than the drug. The photoprotective action of the complex was found to be better than drug on the basis of the content of antioxidant enzymes estimated in the skin.</p><strong>Conclusion: </strong>The phytosomal complex was found to show better solubility in the water phase and oil phase, better permeation, better antioxidant activity and a better photo-protective activity when compared to umbelliferone.

Sign in / Sign up

Export Citation Format

Share Document