scholarly journals The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6005
Author(s):  
Jolanta Flieger ◽  
Michał Flieger
Keyword(s):  
Antioxidant Activity ◽  
Reduced Glutathione ◽  
Array Detector ◽  
Dpph Radical ◽  
Diode Array Detector ◽  
Reverse Phase ◽  
Fast Kinetics ◽  
Ultrasonic Bath ◽  
Rp Hplc ◽  
Scavenging Capacity

The 2,2-diphenyl-1-picrylhydrazyl (DPPH)-reverse phase (RP)-HPLC-diode array detector (DAD) method was tested on standard antioxidants (AOs), i.e., reduced glutathione (GSH), ascorbic acid (vitamin C), and alcoholic extracts of A. podagraria L. An elaborated HPLC procedure enabled the simultaneous measurement of the redox couple DPPH-R (2,2-diphenyl-1-picrylhydrazyl radical)/DPPH-H (2,2-diphenyl-1-picrylhydrazine). Both forms were fully separated (Rs = 2.30, α = 1.65) on a Zorbax Eclipse XDB-C18 column eluted with methanol–water (80:20, v/v) and detected at different wavelengths in the range of 200–600 nm. The absorbance increases of the DPPH-H as well as the DPPH-R peak inhibition were measured at different wavelengths in visible and UV ranges. The chromatographic method was optimized, according to reaction time (slow, fast kinetics), the linearity range of DPPH radical depending on the detection conditions as well as the kind of the investigated antioxidants (reference chemicals and the ground elder prepared from fresh and dry plants). The scavenging capacity was expressed by the use of percentage of peak inhibition and the IC50 parameters. The evaluated extracts displayed antioxidant activity, higher than 20% inhibition against 350 µM DPPH free radical. The results show that extract prepared from dry plants in the ultrasonic bath exhibits the highest antioxidant potential (IC50 = 64.74 ± 0.22 µL/mL).

scholarly journals DEVELOPMENT AND STABILITY INDICATING HPLC METHOD FOR DAPAGLIFLOZIN IN API AND PHARMACEUTICAL DOSAGE FORM

2017 ◽  
Vol 9 (5) ◽  
pp. 33 ◽  
Author(s):  
Mitali V. Verma ◽  
Chirag J. Patel ◽  
M. M. Patel
Keyword(s):  
Dosage Form ◽  
Hplc Method ◽  
Assay Method ◽  
Array Detector ◽  
Diode Array Detector ◽  
Hydrogen Phosphate ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Relative Standard ◽  
Potassium Hydrogen

Objective: To develop precise, accurate and reproducible stability assay method by RP-HPLC for estimation of dapagliflozin in API and pharmaceutical dosage form.Methods: The adequate separation was carried using agilent C18 (4.6 ml (millimeter)*150,5 µm (micromiter), mixture of acetonitrile: di-potassium hydrogen phosphate with pH-6.5 adjusted with OPA (40:60 %v/v) as a mobile phase with the flow rate of 1 ml/min (milliliter/minute) and the effluent was monitored at 222 nm (nanometer) using photo diode array detector. The retention time of dapagliflozin API and dapagliflozin tablet were 3.160 min (minute) and 3.067 min (minute) respectively.Results: Linearity for dapagliflozin was found in the range of 50-150µg/ml (microgram/milliliter) (R2 = 0.99) respectively. The accuracy of the present method was evaluated at 50 %, 100% and 150%. The % recoveries of dapagliflozin API and tablet were found to be in the range of 99.00–99.99 % and 98.50–99.99 % respectively. Precision studies were carried out and the relative standard deviation values were less than two. The method was found to be robust.Conclusion: The proposed method was found to be specific, accurate, precise and robust can be used for estimation of dapagliflozin in API and Pharmaceutical dosage form.

scholarly journals Total Phenolic Content and Antioxidant Activity of Salvia spp. Exudates

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Laura Giamperi ◽  
Anahi Bucchini ◽  
Angela Bisio ◽  
Emanuela Giacomelli ◽  
Giovanni Romussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

The total phenolic content and antioxidant activity of 6 Salvia spp. exudates were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by a modified Prussian blue method, and the antioxidant activity by two methods: 1,1- diphenyl-2-picryl- hydrazyl (DPPH) radical scavenging capacity assay and lipoxygenase inhibitory assay. The total phenolic content ranged between 1.3 μg/mg DW ( S. fallax) and 74.0 μg/mg DW ( S. cacaliaefolia). In the DPPH test, S. cacaliaefolia was more effective than BHT, while in the inhibition of lipid peroxidation all the extracts presented good antioxidant capacity.

scholarly journals Authentication of the Geographical Origin of Margarines and Fat-Spread Products from Liquid Chromatographic UV-Absorption Fingerprints and Chemometrics

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 588 ◽  
Author(s):  
Sanae Bikrani ◽  
Ana M. Jiménez-Carvelo ◽  
Mounir Nechar ◽  
M. Gracia Bagur-González ◽  
Badredine Souhail ◽  
...  
Keyword(s):  
Geographical Origin ◽  
Array Detector ◽  
Diode Array ◽  
Sample Treatment ◽  
Classification Methods ◽  
Diode Array Detector ◽  
Reverse Phase ◽  
Informative Signal ◽  
And Performance ◽  
Liquid Chromatographic

Fat-spread products are a stabilized emulsion of water and vegetable oils. The whole fat content can vary from 10 to 90% (w/w). There are different kinds, which are differently named, and their composition depends on the country in which they are produced or marketed. Thus, having analytical solutions to determine geographical origin is required. In this study, some multivariate classification methods are developed and optimised to differentiate fat-spread-related products from different geographical origins (Spain and Morocco), using as an analytical informative signal the instrumental fingerprints, acquired by liquid chromatography coupled with a diode array detector (HPLC-DAD) in both normal and reverse phase modes. No sample treatment was applied, and, prior to chromatographic analysis, only the samples were dissolved in n‑hexane. Soft independent modelling of class analogy (SIMCA) and partial least squares-discriminant analysis (PLS-DA) were used as classification methods. In addition, several classification strategies were applied, and performance of the classifications was evaluated applying proper classification metrics. Finally, 100% of samples were correctly classified applying PLS-DA with data collected in reverse phase.

Characterization of conjugated linoleic acid and its metabolites by RP-HPLC with diode array detector

2001 ◽  
Vol 103 (9) ◽  
pp. 617-621 ◽  
Author(s):  
Maria Paola Melis ◽  
Elisabetta Angioni ◽  
Gianfranca Carta ◽  
Elisabetta Murru ◽  
Paolo Scanu ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Rp Hplc

scholarly journals Antioxidant, antifungal and anticancer activities of se-enriched Pleurotus spp. mycelium extracts

2014 ◽  
Vol 66 (4) ◽  
pp. 1379-1388 ◽  
Author(s):  
Ivan Milovanovic ◽  
Mirjana Stajic ◽  
Jasmina Cilerdzic ◽  
Tatjana Stanojkovic ◽  
Aleksandar Knezevic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Radical Scavenging ◽  
Pleurotus Eryngii ◽  
Dpph Radical ◽  
Anticancer Activities ◽  
Radical Scavenging Capacity ◽  
Low Concentrations ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

The goal of this study was the evaluation of antifungal, antioxidant and anticancer potentials of Pleurotus eryngii, P. ostreatus and P. pulmonarius mycelial extracts, and the influence of mycelium enrichment with selenium on these activities. Both Se-amended and non-amended extracts showed the same or similar minimal inhibitory concentration for 14 studied micromycetes, while a fungicidal effect was not noted, contrary to ketoconazole, which had inhibitory and fungicidal effects at very low concentrations. Se-non-amended extracts exhibited antioxidant activity, especially at higher concentrations. Selenium enrichment influenced activity, its effects decreasing in P. eryngii and P. pulmonarius, while in P. ostreatus no effect was noted. The DPPH? radical scavenging capacity of the extracts was in direct correlation with their phenol and flavonoid contents. Cytotoxic activity against both HeLa and LS174 cell lines was very low compared with cis-DDP. These features suggest that mycelium should be an object of intensive studies.

Application of RP-HPLC-Diode Array Detector after SPE to the Determination of Pesticides in Pepper Samples

2012 ◽  
Vol 95 (5) ◽  
pp. 1357-1361 ◽  
Author(s):  
Tomasz Tuzimski
Keyword(s):  
Quantitative Analysis ◽  
Plant Material ◽  
Food Samples ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Green Pepper ◽  
Rp Hplc ◽  
Chemical Groups

Abstract The application of HPLC-diode array detector (DAD) after SPE for identification and quantitative analysis of pesticides in red and green pepper samples is demonstrated. An HPLC procedure on an RP column (C18) was developed for analysis of selected pesticides from different chemical groups: metamitron, metalaxyl, linuron, and prometryn. Average recoveries for C18 Polar Plus cartridges and solvents by the proposed RP-HPLC-DAD method after SPE are presented. Average recoveries from the spiked samples and the SDs were 22.5 ± 2.2, 138.0 ± 4.1, 78.6 ± 2.8, and 109.2 ± 2.3% for metamitron, metalaxyl, linuron, and prometryn, respectively, at concentrations of 7 μg/g in the plant material. The efficiency of the SPE procedure was evaluated using real food samples. The quantities of prometryn, linuron, metalaxyl, and metamitron determined were in the ranges of 0.02–2.24 μg/g (n = 24), 0.08–1.01 μg/g (n = 9), 1.61–2.28 μg/g (n = 4), and 0.05–1.07 μg/g (n = 3), respectively, in plant material sampled in 2011. The method was validated for precision, repeatability, and accuracy.

Sign in / Sign up

Export Citation Format

Share Document