61. A Comparison of the Andersen Single-Stage (N-6) Sampler with the Rotorod Spore Trap in Test Atmosphere Containing Viable and Non-Viable Fungal Spores

2001 ◽  
Author(s):  
B. Samimi ◽  
L. Brenner ◽  
J. Quintana
2021 ◽  
Vol 34 ◽  
pp. 04018
Author(s):  
Oksana Kremneva ◽  
Svetlana Nekoval ◽  
Alexey Pachkin ◽  
Anastasia Zakharchenko ◽  
Ksenia Gasiyan

The effect of the biofungicide BFTIM KS-2, Zh on the development and spreading of the Alternaria alternata fungus on vegetable crops (tomato, pepper, eggplant) was studied using classical and modern methods of phytosanitary monitoring. The research was carried out in Krasnodar region in the Pavlovsky District, on the production fields of the ZAO Yubileinoye. To assess the development and spreading of the disease, the classical visual method was used, as well as a modern method for determining the congestion rate of plantings using the OZR-1mp spore trap.The article presents data on the development and spreading of A. alternata, the number of fungal spores on vegetable crops before and after treatments with the biofungicide. It was found that the use of the biological product reduces the development and spreading of the phytopathogen. Its influence is the most effective on tomato and eggplant crops. Also, in the course of research, the possibility of using a spore trap for monitoring Alternaria on vegetable crops has been shown.


2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S38-S42
Author(s):  
R. Kennedy ◽  
A.J. Wakeham

Information on the presence or absence of airborne spores or other particles would be useful in an increasing number of areas including agriculture. Traditional methods used for detecting and enumerating of airborne spores of fungal plant pathogens are time consuming and require specialist knowledge. Some spore types (e.g. ascospores) are difficult to differentiate using these methods. To facilitate this, new methods, which can be used to accurately differentiate fungal spore types, are required. A Burkard 7-day volumetric spore trap used in combination with an immunofluorescence test has been developed to detect and quantify field-trapped ascosporic inoculum of Mycosphaerella brassicicola (the ringspot pathogen of brassicas). This test has also been found useful in the validation of more rapid user-friendly immunoassay based trapping procedures. A microtiter immunospore trapping device, which uses a suction system to directly trap air-particulates by impaction into microtiter wells, has been used successfully for the rapid detection and quantification of ascosporic inoculum of M. brassicicola. The system shows potential for the rapid field-detection of airborne ascosporic inoculum of the ringspot pathogen.


1974 ◽  
Vol 20 (5) ◽  
pp. 721-729 ◽  
Author(s):  
D. Brewer ◽  
A. Taylor ◽  
M. M. Hoehn

The air and herbage fungal flora of permanent pasture at Nappan, Nova Scotia, has been sampled by means of a mobile spore trap during the spring, summer, and fall of 1967, 1968, 1969, 1970, and 1972. In 1968 and 1972 a random sample of the isolates collected was grown in the laboratory and the cultures obtained screened for their ability to inhibit the growth of bacteria, fungi, and algae. In 1967, 1968, and 1972 about 25% of the isolates tested produced metabolites that were inhibitory to growth. The summer efflorescence observed in all 5 years was not accompanied by a corresponding surge of antibiotic-producing organisms, except in the early summer and fall. Five of the six most commonly found species, when collected in the fall, were more likely to produce antibiotics than the same species isolated in the spring and early summer. This changing faculty of this fungal population to produce antibiotics as the season advances was observed whether bacteria, fungi, or algae were used as test organisms. The results appear to provide experimental evidence for the utility of antibiotic production for survival.


2004 ◽  
Vol 33 (3) ◽  
pp. 393 ◽  
Author(s):  
Heike G. Neumeister-Kemp ◽  
Aaron Maxwell ◽  
Peter C. Kemp ◽  
Bernie Dell ◽  
Giles E. St J. Hardy
Keyword(s):  

2019 ◽  
Vol 11 (14) ◽  
pp. 1671 ◽  
Author(s):  
Roland Sarda-Estève ◽  
Dominique Baisnée ◽  
Benjamin Guinot ◽  
John Sodeau ◽  
David O’Connor ◽  
...  

Airborne fungal spores (AFS) represent the major fraction of primary biological aerosol particles (PBAPs), and they are studied worldwide largely due to their important role within the Earth system. They have an impact on climate and human health, and they contribute to the propagation of diseases. As their presence in the air depends largely on studied ecosystems, a spore trap was used to monitor their atmospheric concentrations from 2014 to December 2018 in Saclay, a suburban area in the megacity of Paris. The main objective of this work was: (1) to understand the atmospheric variability of AFS in relation to different variables such as meteorological factors, agricultural practice, and (2) to identify their geographical origin by using a source receptor model. During our period of observation, 30 taxa have been identified under a light microscope. In order of importance, Ascospores, Cladosporium, Basidiospores, Tilletiopsis, Alternaria were found to be the most abundant types respectively (50.8%, 33.6%, 7.6%, 1.8%, and 1.3%) accounting for 95% of the atmospheric concentrations. We observed a general decrease associated with a strong interannual variability. A bimodal seasonal cycle was identified with a first maximum in July and a second in October. The main parameters driving the atmospheric concentration are temperature and precipitation. The daily variability is strongly activated by successive periods of hot weather and rainfall, multiplying the concentration by a factor of 1000 in less than 12 hours. Results from the source receptor model ZeFir point out unambiguous different origins of AFS due to specific sources impacting the observation site. Our study also indicated that a hydrological stress has a direct effect on the daily concentrations. This last point should be taken into account for every stressed ecosystem studied in a global warming context. This is particularly important for Mediterranean areas where water is a key control of the growth and dispersion of fungal spores.


2007 ◽  
Vol 177 (4S) ◽  
pp. 12-12
Author(s):  
L. Andrew Evans ◽  
Benjamin Moses ◽  
Kevin Rice ◽  
Craig Robson ◽  
Allen F. Morey

1989 ◽  
Vol 50 (C1) ◽  
pp. C1-813-C1-817
Author(s):  
M. ARNOULD ◽  
F. BAETEN ◽  
D. DARQUENNES ◽  
Th. DELBAR ◽  
C. DOM ◽  
...  

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