Development of decellularized meniscus extracellular matrix and gelatin/chitosan scaffolds for meniscus tissue engineering

2019 ◽  
Vol 30 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Zhang Yu ◽  
Jiang Lili ◽  
Zheng Tiezheng ◽  
Sha Li ◽  
Wang Jianzhuang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Meniscus Tissue ◽  
Chitosan Scaffolds

Autologous Human Fibrochondrocytes From Meniscectomy Debris are a Potent Cell Source for Meniscus Tissue Engineering

2007 ◽  
Author(s):  
Brendon M. Baker ◽  
Ashwin S. Nathan ◽  
Neil P. Sheth ◽  
G. Russell Huffman ◽  
Robert L. Mauck
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Normal Functioning ◽  
Arthroscopic Partial Meniscectomy ◽  
Load Transmission ◽  
Articular Surfaces ◽  
Meniscus Tissue ◽  
Cell Source ◽  
Inner Region ◽  
Collagenous Structure

The meniscus is a fibrocartilaginous tissue vital to the normal functioning of the knee [1]. The dense collagenous structure is sparsely colonized by meniscal fibrochondrocytes (MFCs) which maintain and remodel the extracellular matrix (ECM) [2,3]. While the meniscus functions well with a lifetime of use, traumatic or degenerative injuries to the avascular, inner region of the meniscus fail to heal. Disruption of the fibrous architecture impairs load transmission and initiates erosion of the adjacent articular surfaces, or osteoarthritis (OA). Damage to the meniscus is typically treated by resection of the torn tissue via arthroscopic partial meniscectomy, which alleviates symptoms but similarly predisposes patients to OA [4]. Tissue removed in this procedure is deemed surgical waste and is subsequently discarded.

scholarly journals 3D Printed Poly(ε-Caprolactone)/Meniscus Extracellular Matrix Composite Scaffold Functionalized With Kartogenin-Releasing PLGA Microspheres for Meniscus Tissue Engineering

2021 ◽  
Vol 9 ◽  
Author(s):  
Hao Li ◽  
Zhiyao Liao ◽  
Zhen Yang ◽  
Cangjian Gao ◽  
Liwei Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Composite Scaffold ◽  
Prolonged Release ◽  
Plga Microspheres ◽  
Cell Vitality ◽  
Meniscal Tissue ◽  
Meniscus Tissue ◽  
Meniscus Regeneration

Meniscus tissue engineering (MTE) aims to fabricate ideal scaffolds to stimulate the microenvironment for recreating the damaged meniscal tissue. Indeed, favorable mechanical properties, suitable biocompatibility, and inherent chondrogenic capability are crucial in MTE. In this study, we present a composite scaffold by 3D printing a poly(ε-caprolactone) (PCL) scaffold as backbone, followed by injection with the meniscus extracellular matrix (MECM), and modification with kartogenin (KGN)-loaded poly(lactic-co-glycolic) acid (PLGA) microsphere (μS), which serves as a drug delivery system. Therefore, we propose a plan to improve meniscus regeneration via KGN released from the 3D porous PCL/MECM scaffold. The final results showed that the hydrophilicity and bioactivity of the resulting PCL/MECM scaffold were remarkably enhanced. In vitro synovium-derived mesenchymal stem cells (SMSCs) experiments suggested that introducing MECM components helped cell adhesion and proliferation and maintained promising ability to induce cell migration. Moreover, KGN-incorporating PLGA microspheres, which were loaded on scaffolds, showed a prolonged release profile and improved the chondrogenic differentiation of SMSCs during the 14-day culture. Particularly, the PCL/MECM-KGN μS seeded by SMSCs showed the highest secretion of total collagen and aggrecan. More importantly, the synergistic effect of the MECM and sustained release of KGN can endow the PCL/MECM-KGN μS scaffolds with not only excellent cell affinity and cell vitality preservation but also chondrogenic activity. Thus, the PCL/MECM-KGN μS scaffolds are expected to have good application prospects in the field of MTE.

Fabrication and characterization of electrospun nanofibers composed of decellularized meniscus extracellular matrix and polycaprolactone for meniscus tissue engineering

2017 ◽  
Vol 5 (12) ◽  
pp. 2273-2285 ◽  
Author(s):  
Shuang Gao ◽  
Weimin Guo ◽  
Mingxue Chen ◽  
Zhiguo Yuan ◽  
Mingjie Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Electrospun Nanofibers ◽  
Meniscus Tissue ◽  
Fabrication And Characterization ◽  
Meniscus Scaffolds

Decellularized meniscus extracellular matrix (DMECM) and polycaprolactone (PCL) were electrospun into nanofibers to make meniscus scaffolds with good mechanical properties.

scholarly journals Porous scaffolds with the structure of an interpenetrating polymer network made by gelatin methacrylated nanoparticle-stabilized high internal phase emulsion polymerization targeted for tissue engineering

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22544-22555
Author(s):  
Atefeh Safaei-Yaraziz ◽  
Shiva Akbari-Birgani ◽  
Nasser Nikfarjam
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Polymer Network ◽  
Synthetic Polymers ◽  
Tissue Scaffolds ◽  
Interpenetrating Polymer Network ◽  
Porous Scaffolds ◽  
Promising Strategy ◽  
Internal Phase

The interlacing of biopolymers and synthetic polymers is a promising strategy to fabricate hydrogel-based tissue scaffolds to biomimic a natural extracellular matrix for cell growth.

Optimization and evaluation of ciprofloxacin-loaded collagen/chitosan scaffolds for skin tissue engineering

3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Satyavrat Tripathi ◽  
Bhisham Narayan Singh ◽  
Divakar Singh ◽  
Gaurav kumar ◽  
Pradeep Srivastava
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Chitosan Scaffolds

scholarly journals Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation

2020 ◽  
Vol 6 (3) ◽  
pp. 410-413
Author(s):  
Petra J. Kluger ◽  
Svenja Nellinger ◽  
Simon Heine ◽  
Ann-Cathrin Volz
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipogenic Differentiation ◽  
Cellular Activity ◽  
Adipose Tissue Engineering ◽  
Physical Support ◽  
Supporting Effect ◽  
Brdu Assay ◽  
The Impact

AbstractThe extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.

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