scholarly journals Meniscus tissue engineering using a novel combination of electrospun scaffolds and human meniscus cells embedded within an extracellular matrix hydrogel

2015 ◽  
Vol 33 (4) ◽  
pp. 572-583 ◽  
Author(s):  
Jihye Baek ◽  
Xian Chen ◽  
Sujata Sovani ◽  
Sungho Jin ◽  
Shawn P. Grogan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Electrospun Scaffolds ◽  
Meniscus Tissue ◽  
Meniscus Cells

scholarly journals Harnessing the Topography of 3D Spongy-Like Electrospun Bundled Fibrous Scaffold via a Sharply Inclined Array Collector

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1444 ◽  
Author(s):  
Sun Hee Cho ◽  
Jeong In Kim ◽  
Cheol Sang Kim ◽  
Chan Hee Park ◽  
In Gi Kim
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Living System ◽  
Pivotal Role ◽  
Skin Tissue ◽  
Target Tissue ◽  
Fibrous Scaffold ◽  
Electrospun Scaffolds ◽  
Directional Change

To date, many researchers have studied a considerable number of three-dimensional (3D) cotton-like electrospun scaffolds for tissue engineering, including the generation of bone, cartilage, and skin tissue. Although numerous 3D electrospun fibrous matrixes have been successfully developed, additional research is needed to produce 3D patterned and sophisticated structures. The development of 3D fibrous matrixes with patterned and sophisticated structures (FM-PSS) capable of mimicking the extracellular matrix (ECM) is important for advancing tissue engineering. Because modulating nano to microscale features of the 3D fibrous scaffold to control the ambient microenvironment of target tissue cells can play a pivotal role in inducing tissue morphogenesis after transplantation in a living system. To achieve this objective, the 3D FM-PSSs were successfully generated by the electrospinning using a directional change of the sharply inclined array collector. The 3D FM-PSSs overcome the current limitations of conventional electrospun cotton-type 3D matrixes of random fibers.

Autologous Human Fibrochondrocytes From Meniscectomy Debris are a Potent Cell Source for Meniscus Tissue Engineering

2007 ◽  
Author(s):  
Brendon M. Baker ◽  
Ashwin S. Nathan ◽  
Neil P. Sheth ◽  
G. Russell Huffman ◽  
Robert L. Mauck
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Normal Functioning ◽  
Arthroscopic Partial Meniscectomy ◽  
Load Transmission ◽  
Articular Surfaces ◽  
Meniscus Tissue ◽  
Cell Source ◽  
Inner Region ◽  
Collagenous Structure

The meniscus is a fibrocartilaginous tissue vital to the normal functioning of the knee [1]. The dense collagenous structure is sparsely colonized by meniscal fibrochondrocytes (MFCs) which maintain and remodel the extracellular matrix (ECM) [2,3]. While the meniscus functions well with a lifetime of use, traumatic or degenerative injuries to the avascular, inner region of the meniscus fail to heal. Disruption of the fibrous architecture impairs load transmission and initiates erosion of the adjacent articular surfaces, or osteoarthritis (OA). Damage to the meniscus is typically treated by resection of the torn tissue via arthroscopic partial meniscectomy, which alleviates symptoms but similarly predisposes patients to OA [4]. Tissue removed in this procedure is deemed surgical waste and is subsequently discarded.

scholarly journals 3D Printed Poly(ε-Caprolactone)/Meniscus Extracellular Matrix Composite Scaffold Functionalized With Kartogenin-Releasing PLGA Microspheres for Meniscus Tissue Engineering

2021 ◽  
Vol 9 ◽  
Author(s):  
Hao Li ◽  
Zhiyao Liao ◽  
Zhen Yang ◽  
Cangjian Gao ◽  
Liwei Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Composite Scaffold ◽  
Prolonged Release ◽  
Plga Microspheres ◽  
Cell Vitality ◽  
Meniscal Tissue ◽  
Meniscus Tissue ◽  
Meniscus Regeneration

Meniscus tissue engineering (MTE) aims to fabricate ideal scaffolds to stimulate the microenvironment for recreating the damaged meniscal tissue. Indeed, favorable mechanical properties, suitable biocompatibility, and inherent chondrogenic capability are crucial in MTE. In this study, we present a composite scaffold by 3D printing a poly(ε-caprolactone) (PCL) scaffold as backbone, followed by injection with the meniscus extracellular matrix (MECM), and modification with kartogenin (KGN)-loaded poly(lactic-co-glycolic) acid (PLGA) microsphere (μS), which serves as a drug delivery system. Therefore, we propose a plan to improve meniscus regeneration via KGN released from the 3D porous PCL/MECM scaffold. The final results showed that the hydrophilicity and bioactivity of the resulting PCL/MECM scaffold were remarkably enhanced. In vitro synovium-derived mesenchymal stem cells (SMSCs) experiments suggested that introducing MECM components helped cell adhesion and proliferation and maintained promising ability to induce cell migration. Moreover, KGN-incorporating PLGA microspheres, which were loaded on scaffolds, showed a prolonged release profile and improved the chondrogenic differentiation of SMSCs during the 14-day culture. Particularly, the PCL/MECM-KGN μS seeded by SMSCs showed the highest secretion of total collagen and aggrecan. More importantly, the synergistic effect of the MECM and sustained release of KGN can endow the PCL/MECM-KGN μS scaffolds with not only excellent cell affinity and cell vitality preservation but also chondrogenic activity. Thus, the PCL/MECM-KGN μS scaffolds are expected to have good application prospects in the field of MTE.

Development of decellularized meniscus extracellular matrix and gelatin/chitosan scaffolds for meniscus tissue engineering

2019 ◽  
Vol 30 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Zhang Yu ◽  
Jiang Lili ◽  
Zheng Tiezheng ◽  
Sha Li ◽  
Wang Jianzhuang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Meniscus Tissue ◽  
Chitosan Scaffolds

scholarly journals Biofabrication of Electrospun Scaffolds for the Regeneration of Tendons and Ligaments

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1963 ◽  
Author(s):  
Alberto Sensini ◽  
Luca Cristofolini
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Electrospun Scaffolds ◽  
General Overview ◽  
Different Types ◽  
Non Linear ◽  
Nanometric Size ◽  
Ligament Tissue Engineering

Tendon and ligament tissue regeneration and replacement are complex since scaffolds need to guarantee an adequate hierarchical structured morphology, and non-linear mechanical properties. Moreover, to guide the cells’ proliferation and tissue re-growth, scaffolds must provide a fibrous texture mimicking the typical of the arrangement of the collagen in the extracellular matrix of these tissues. Among the different techniques to produce scaffolds, electrospinning is one of the most promising, thanks to its ability to produce fibers of nanometric size. This manuscript aims to provide an overview to researchers approaching the field of repair and regeneration of tendons and ligaments. To clarify the general requirements of electrospun scaffolds, the first part of this manuscript presents a general overview concerning tendons’ and ligaments’ structure and mechanical properties. The different types of polymers, blends and particles most frequently used for tendon and ligament tissue engineering are summarized. Furthermore, the focus of the review is on describing the different possible electrospinning setups and processes to obtain different nanofibrous structures, such as mats, bundles, yarns and more complex hierarchical assemblies. Finally, an overview concerning how these technologies are exploited to produce electrospun scaffolds for tendon and ligament tissue applications is reported together with the main findings and outcomes.

scholarly journals A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size

2017 ◽  
Vol 2 (1) ◽  
pp. 46-61 ◽  
Author(s):  
Kevin P. Feltz ◽  
Emily A. Growney Kalaf ◽  
Chengpeng Chen ◽  
R. Scott Martin ◽  
Scott A. Sell
Keyword(s):  
Magnetic Field ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Electrospinning Process ◽  
Electrospun Scaffolds ◽  
Alternative Techniques ◽  
Fiber Deposition ◽  
Medicine Community ◽  
Field Manipulation

Abstract Electrospinning has been widely accepted for several decades by the tissue engineering and regenerative medicine community as a technique for nanofiber production. Owing to the inherent flexibility of the electrospinning process, a number of techniques can be easily implemented to control fiber deposition (i.e. electric/ magnetic field manipulation, use of alternating current, or air-based fiber focusing) and/or porosity (i.e. air impedance, sacrificial porogen/sacrificial fiber incorporation, cryo-electrospinning, or alternative techniques). The purpose of this review is to highlight some of the recent work using these techniques to create electrospun scaffolds appropriate for mimicking the structure of the native extracellular matrix, and to enhance the applicability of advanced electrospinning techniques in the field of tissue engineering.

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