scholarly journals Porous scaffolds with the structure of an interpenetrating polymer network made by gelatin methacrylated nanoparticle-stabilized high internal phase emulsion polymerization targeted for tissue engineering

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22544-22555
Author(s):  
Atefeh Safaei-Yaraziz ◽  
Shiva Akbari-Birgani ◽  
Nasser Nikfarjam
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Polymer Network ◽  
Synthetic Polymers ◽  
Tissue Scaffolds ◽  
Interpenetrating Polymer Network ◽  
Porous Scaffolds ◽  
Promising Strategy ◽  
Internal Phase

The interlacing of biopolymers and synthetic polymers is a promising strategy to fabricate hydrogel-based tissue scaffolds to biomimic a natural extracellular matrix for cell growth.

scholarly journals Macroporous chitosan/methoxypoly(ethylene glycol) based cryosponges with unique morphology for tissue engineering applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pradeep Kumar ◽  
Viness Pillay ◽  
Yahya E. Choonara
Keyword(s):  
Tissue Engineering ◽  
Polymer Network ◽  
Three Dimensional ◽  
Hysteresis Loops ◽  
Interpenetrating Polymer Network ◽  
Morphological Data ◽  
Porous Scaffolds ◽  
Morphological Variations ◽  
Molecular Stability ◽  
The Matrix

AbstractThree-dimensional porous scaffolds are widely employed in tissue engineering and regenerative medicine for their ability to carry bioactives and cells; and for their platform properties to allow for bridging-the-gap within an injured tissue. This study describes the effect of various methoxypolyethylene glycol (mPEG) derivatives (mPEG (-OCH3 functionality), mPEG-aldehyde (mPEG-CHO) and mPEG-acetic acid (mPEG-COOH)) on the morphology and physical properties of chemically crosslinked, semi-interpenetrating polymer network (IPN), chitosan (CHT)/mPEG blend cryosponges. Physicochemical and molecular characterization revealed that the –CHO and –COOH functional groups in mPEG derivatives interacted with the –NH2 functionality of the chitosan chain. The distinguishing feature of the cryosponges was their unique morphological features such as fringe thread-, pebble-, curved quartz crystal-, crystal flower-; and canyon-like structures. The morphological data was well corroborated by the image processing data and physisorption curves corresponding to Type II isotherm with open hysteresis loops. Functionalization of mPEG had no evident influence on the macro-mechanical properties of the cryosponges but increased the matrix strength as determined by the rheomechanical analyses. The cryosponges were able to deliver bioactives (dexamethasone and curcumin) over 10 days, showed varied matrix degradation profiles, and supported neuronal cells on the matrix surface. In addition, in silico simulations confirmed the compatibility and molecular stability of the CHT/mPEG blend compositions. In conclusion, the study confirmed that significant morphological variations may be induced by minimal functionalization and crosslinking of biomaterials.

scholarly journals Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering

2021 ◽  
pp. 088532822110240
Author(s):  
Nastaran Shojarazavi ◽  
Shohreh Mashayekhan ◽  
Hossein Pazooki ◽  
Sadaf Mohsenifard ◽  
Hossein Baniasadi
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Polymer Network ◽  
Cartilage Tissue Engineering ◽  
Compression Modulus ◽  
Interpenetrating Polymer Network ◽  
Cartilage Tissue ◽  
Cartilage Extracellular Matrix ◽  
Capacity Degradation ◽  
Alginate Concentration

In the present study, alginate/cartilage extracellular matrix (ECM)-based injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) for cartilage tissue engineering. The in situ forming hydrogels were composed of different ionic crosslinked alginate concentrations with 1% w/v enzymatically crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer network (IPN). The response surface methodology (RSM) approach was applied to optimize IPN hydrogel's mechanical properties by varying alginate and SFN concentrations. The results demonstrated that upon increasing the alginate concentration, the compression modulus improved. The SFN concentration was optimized to reach a desired mechanical stiffness. Accordingly, the concentrations of alginate and SFN to have an optimum compression modulus in the hydrogel were found to be 1.685 and 1.724% w/v, respectively. The gelation time was found to be about 10 s for all the samples. Scanning electron microscope (SEM) images showed homogeneous dispersion of the SFN in the hydrogel, mimicking the natural cartilage environment. Furthermore, water uptake capacity, degradation rate, cell cytotoxicity, and glycosaminoglycan and collagen II secretions were determined for the optimum hydrogel to support its potential as an injectable scaffold for articular cartilage defects.

scholarly journals Polymeric Scaffolds in Tissue Engineering Application: A Review

2011 ◽  
Vol 2011 ◽  
pp. 1-19 ◽  
Author(s):  
Brahatheeswaran Dhandayuthapani ◽  
Yasuhiko Yoshida ◽  
Toru Maekawa ◽  
D. Sakthi Kumar
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Application ◽  
Biologically Active ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Tissue Engineering Scaffolds ◽  
Matrix Deposition ◽  
Bioactive Agents ◽  
Regenerate Tissue

Current strategies of regenerative medicine are focused on the restoration of pathologically altered tissue architectures by transplantation of cells in combination with supportive scaffolds and biomolecules. In recent years, considerable interest has been given to biologically active scaffolds which are based on similar analogs of the extracellular matrix that have induced synthesis of tissues and organs. To restore function or regenerate tissue, a scaffold is necessary that will act as a temporary matrix for cell proliferation and extracellular matrix deposition, with subsequent ingrowth until the tissues are totally restored or regenerated. Scaffolds have been used for tissue engineering such as bone, cartilage, ligament, skin, vascular tissues, neural tissues, and skeletal muscle and as vehicle for the controlled delivery of drugs, proteins, and DNA. Various technologies come together to construct porous scaffolds to regenerate the tissues/organs and also for controlled and targeted release of bioactive agents in tissue engineering applications. In this paper, an overview of the different types of scaffolds with their material properties is discussed. The fabrication technologies for tissue engineering scaffolds, including the basic and conventional techniques to the more recent ones, are tabulated.

scholarly journals Engineering a macroporous fibrin-based sequential interpenetrating polymer network for dermal tissue engineering

2020 ◽  
Vol 8 (24) ◽  
pp. 7106-7116
Author(s):  
Olfat Gsib ◽  
Loek J. Eggermont ◽  
Christophe Egles ◽  
Sidi A. Bencherif
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Biological Activity ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Dermal Tissue

Macroporous and mechanically reinforced sequential IPN hydrogels combine the biological activity of fibrin with the robust mechanical properties of PEG to generate advanced scaffolds for dermal tissue engineering.

scholarly journals Marine Biomaterial-Based Bioinks for Generating 3D Printed Tissue Constructs

Marine Drugs ◽  
2018 ◽  
Vol 16 (12) ◽  
pp. 484 ◽  
Author(s):  
Xiaowei Zhang ◽  
Gyeong Kim ◽  
Min Kang ◽  
Jung Lee ◽  
Jeong Seo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Physical Properties ◽  
Degradation Rate ◽  
Polymer Network ◽  
Three Dimensional ◽  
Pharmaceutical Products ◽  
Biologically Active ◽  
Interpenetrating Polymer Network ◽  
Biomedical Fields

Biologically active materials from marine sources have been receiving increasing attention as they are free from the transmissible diseases and religious restrictions associated with the use of mammalian resources. Among various other biomaterials from marine sources, alginate and fish gelatin (f-gelatin), with their inherent bioactivity and physicochemical tunability, have been studied extensively and applied in various biomedical fields such as regenerative medicine, tissue engineering, and pharmaceutical products. In this study, by using alginate and f-gelatin’s chemical derivatives, we developed a marine-based interpenetrating polymer network (IPN) hydrogel consisting of alginate and f-gelatin methacryloyl (f-GelMA) networks via physical and chemical crosslinking methods, respectively. We then evaluated their physical properties (mechanical strength, swelling degree, and degradation rate) and cell behavior in hydrogels. Our results showed that the alginate/f-GelMA hydrogel displayed unique physical properties compared to when alginate and f-GelMA were used separately. These properties included high mechanical strength, low swelling and degradation rate, and an increase in cell adhesive ability. Moreover, for the first time, we introduced and optimized the application of alginate/f-GelMA hydrogel in a three-dimensional (3D) bioprinting system with high cell viability, which breaks the restriction of their utilization in tissue engineering applications and suggests that alginate/f-GelMA can be utilized as a novel bioink to broaden the uses of marine products in biomedical fields.

Fmoc-FF and hexapeptide-based multicomponent hydrogels as scaffold materials

Soft Matter ◽  
2019 ◽  
Vol 15 (3) ◽  
pp. 487-496 ◽  
Author(s):  
Carlo Diaferia ◽  
Moumita Ghosh ◽  
Teresa Sibillano ◽  
Enrico Gallo ◽  
Mariano Stornaiuolo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Building Blocks ◽  
Short Peptides ◽  
Scaffold Materials

Short peptides or single amino acids are interesting building blocks for fabrication of hydrogels, frequently used as extracellular matrix-mimicking scaffolds for cell growth in tissue engineering.

Interpenetrating polymer network hydrogels as bioactive scaffolds for tissue engineering

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Cody O. Crosby ◽  
Brett Stern ◽  
Nikhith Kalkunte ◽  
Shahar Pedahzur ◽  
Shreya Ramesh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Disease Modeling ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Health It ◽  
Bioactive Scaffolds ◽  
3D Microenvironment ◽  
Patient Health ◽  
Biomaterial Scaffolds ◽  
Superior Mechanical Properties

AbstractTissue engineering, after decades of exciting progress and monumental breakthroughs, has yet to make a significant impact on patient health. It has become apparent that a dearth of biomaterial scaffolds which possess the material properties of human tissue while remaining bioactive and cytocompatible, has been partly responsible for this lack of clinical translation. Herein, we propose the development of interpenetrating polymer network (IPN) hydrogels as materials that can provide cells with an adhesive extracellular matrix-like 3D microenvironment while possessing the mechanical integrity to withstand physiological forces. These hydrogels can be synthesized from biologically derived or synthetic polymers, the former polymer offering preservation of adhesion, degradability, and microstructure and the latter polymer offering tunability and superior mechanical properties. We review critical advances in the enhancement of mechanical strength, substrate-scale stiffness, electrical conductivity, and degradation in IPN hydrogels intended as bioactive scaffolds in the past 5 years. We also highlight the exciting incorporation of IPN hydrogels into state-of-the-art tissue engineering technologies, such as organ-on-a-chip and bioprinting platforms. These materials will be critical in the engineering of functional tissue for transplant, disease modeling and drug screening.

The Effect of Cellulose Nanofibres on Mechanical Properties and Bioactivity of Natural Polymers

2013 ◽  
Vol 1498 ◽  
pp. 85-89
Author(s):  
Ali Negahi Shirazi ◽  
Ali Fathi ◽  
Fariba Dehghani
Keyword(s):  
Mechanical Properties ◽  
Cell Growth ◽  
Mechanical Strength ◽  
Polymer Network ◽  
Interpenetrating Polymer Network ◽  
Nano Particles ◽  
Osteosarcoma Cell ◽  
Natural Polymers ◽  
Cellulose Nanofibres

ABSTRACTNatural polymers, used for hydrogel fabrication, are generally bioactive and provide good environment for cell growth and proliferation. However, these polymers have low mechanical strength. Several approaches have been attempted to improve their mechanical properties such as fabrication of interpenetrating polymer network (IPN) and semi-IPN hydrogels, and also addition of a nano sized fibers or nano-particles. The aim of this study was to investigate the feasibility of using naturally derived nano-fillers such as cellulose nanocrystallines to enhance the mechanical properties of hydrogels. Gelatin methacrylate (GelMA) was used as a protein model for preparation of photo-crosslinked hydrogel. The effects of concentrations of photo initiator and cellulose nanocrystallines (CNC) on the characteristics of hydrogels were examined. In vitro studies showed negligible cytotoxic effect of CNC on human osteosarcoma cell growth when using less than 20 mg/ml CNC. Therefore, it is viable to use this nano-filler for biomedical applications. It was found that the compression modulus of gelatin hydrogel was increased 1.5 fold by addition of 10 mg/ml of CNC. These results demonstrate the high potential of using CNC for tissue engineering applications to enhance the mechanical strength of hydrogels.

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