scholarly journals Hepatitis E virus isolates of genotype 4 recovered from wild boars in the Iriomote Island, Okinawa

Kanzo ◽  
2006 ◽  
Vol 47 (3) ◽  
pp. 161-162 ◽  
Author(s):  
M Nakamura ◽  
K Taira ◽  
A Ohno ◽  
M Taira ◽  
H Sakugawa ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Wild Boars ◽  
Iriomote Island ◽  
Virus Isolates

Analysis of the full-length genome of genotype 4 hepatitis E virus isolates from patients with fulminant or acute self-limited hepatitis E

2006 ◽  
Vol 78 (4) ◽  
pp. 476-484 ◽  
Author(s):  
Jun Inoue ◽  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Tatsuya Aikawa ◽  
Hitoshi Mizuo ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Genotype 4 ◽  
Full Length Genome ◽  
Virus Isolates

scholarly journals Detection and Genomic Analysis of Hepatitis E Virus Isolates from Wild Boars in Gunma Prefecture, Japan

2011 ◽  
Vol 64 (1) ◽  
pp. 67-70
Author(s):  
Taisei ISHIOKA ◽  
Chieko SAKANO ◽  
Toshie MOKUDAI ◽  
Yoko YOKOTA ◽  
Hiroyuki SAKANIWA ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Genomic Analysis ◽  
Hepatitis E ◽  
Wild Boars ◽  
Virus Isolates

scholarly journals Analysis of the full-length genome of hepatitis E virus isolates obtained from wild boars in Japan

2005 ◽  
Vol 86 (12) ◽  
pp. 3321-3326 ◽  
Author(s):  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Kazunori Endo ◽  
Shinji Fujiwara ◽  
Nobuo Sakuma ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Partial Sequence ◽  
Genotype 3 ◽  
Entire Genome ◽  
Wild Boars ◽  
Full Length Sequence ◽  
Full Length Genome ◽  
Virus Isolates

Two (2·3 %) of 87 wild-caught boars in Japan had detectable hepatitis E virus (HEV) RNA. The two boar HEV isolates (wbJTS1 and wbJYG1) obtained in the present study and a previously reported isolate (wbJSG1) whose partial sequence had been determined were sequenced over the entire genome. The wbJSG1, wbJTS1 and wbJYG1 isolates comprised 7225 or 7226 nt, excluding the poly(A) tail, and segregated into genotype 3. They differed by 8·5–11·2 % from each other and by 8·6–18·4 % from 17 reported genotype 3 HEV isolates, including one boar isolate, in the full-length sequence. When compared with 191 reported genotype 3 HEV isolates whose partial sequences were known, these three boar isolates were closer to Japanese isolates than to isolates of non-Japanese origin (89·2±2·6 vs 85·9±2·2 %; P<0·0001). A proportion of wild boars in Japan are infected with markedly heterogeneous HEV strains that are indigenous to Japan and may serve as reservoirs of HEV.

Full-Length Sequences of Two Hepatitis E Virus Isolates Representing an Eastern China-Indigenous Subgroup of Genotype 4

Intervirology ◽  
2007 ◽  
Vol 50 (3) ◽  
pp. 181-189 ◽  
Author(s):  
Masami Koike ◽  
Kazuaki Takahashi ◽  
Shunji Mishiro ◽  
Atsushi Matsui ◽  
Mie Inao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Eastern China ◽  
Hepatitis E ◽  
Full Length ◽  
Genotype 4 ◽  
Virus Isolates

scholarly journals Seroprevalence of Hepatitis E Virus Infection among Blood Donors in Bulgaria

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 492
Author(s):  
Magdalena Baymakova ◽  
Krasimira Terzieva ◽  
Rumen Popov ◽  
Elisaveta Grancharova ◽  
Todor Kundurzhiev ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Elisa Test ◽  
Blood Samples ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Specific Questionnaire ◽  
Positive Results

Hepatitis E virus (HEV) infection is widespread among domestic pigs, industrial swine, and wild boars in Bulgaria. The aim of the current research was to present the HEV seroprevalence among blood donors in Bulgaria. In the present study, 555 blood donors (479 males and 76 females) were enrolled from five districts in the country (Shumen, Pleven, Stara Zagora, Plovdiv, and Sofia districts). All blood samples were tested for anti-HEV IgG using the recomWell HEV IgG ELISA test (Mikrogen GmbH, Neuried, Germany). Each participating donor completed a short, structured, and specific questionnaire to document data on the current study. Anti-HEV IgG positive results were detected in 144 (25.9%) blood donors, including 129 (26.9%) males and 15 (19.7%) females. The established HEV seropositivity was 28.8% (23/80) in Shumen district, 23.2% (22/95) in Pleven district, 27.1% (38/140) in Stara Zagora district, 27.5% (44/160) in Plovdiv district, and 21.3% (17/80) in Sofia district. A high HEV seroprevalence was found for persons who declared that they were general hunters (48.7%; 19/39; p = 0.001) and hunters of wild boars (51.6%; 16/31; p = 0.001). We present the first seroprevalence rates of HEV infection in blood donors from Bulgaria. The results of our research showed high HEV seropositivity among blood donors.

Experimental infection of mongolian gerbils by a genotype 4 strain of swine hepatitis E virus

2009 ◽  
Vol 81 (9) ◽  
pp. 1591-1596 ◽  
Author(s):  
Wengui Li ◽  
Quan Sun ◽  
Ruiping She ◽  
Decheng Wang ◽  
Xinhui Duan ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Mongolian Gerbils ◽  
Genotype 4 ◽  
Swine Hepatitis

scholarly journals Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany

2021 ◽  
Vol 9 (11) ◽  
pp. 2302
Author(s):  
Katja Schilling-Loeffler ◽  
Oliver Viera-Segura ◽  
Victor Max Corman ◽  
Julia Schneider ◽  
Ashish K. Gadicherla ◽  
...  
Keyword(s):  
Cell Culture ◽  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enriched Library ◽  
Genome Copy ◽  
Genotype 3 ◽  
Wild Boars ◽  
Copy Numbers ◽  
Cell Culture Isolation

Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described for human isolates so far. Here, five sera and five liver samples from HEV-RNA-positive wild boar samples were inoculated onto PLC/PRF/5 cells, incubated for 3 months and thereafter passaged for additional 6 weeks. As demonstrated by RT-qPCR, immunofluorescence and immune electron microscopy, virus was successfully isolated from two liver samples, which originally contained high HEV genome copy numbers. Both isolates showed slower growth than the culture-adapted HEV strain 47832c. In contrast to this strain, the isolated strains had no insertions in their hypervariable genome region. Next generation sequencing using an HEV sequence-enriched library enabled full genome sequencing. Strain Wb108/17 belongs to subtype 3f and strain Wb257/17 to a tentative novel subtype recently described in Italian wild boars. The results indicate that HEV can be successfully isolated in cell culture from wild boar samples containing high HEV genome copy numbers. The isolates may be used further to study the zoonotic potential of wild boar-derived HEV subtypes.

scholarly journals Molecular tracing of Japan-indigenous hepatitis E viruses

2006 ◽  
Vol 87 (4) ◽  
pp. 949-954 ◽  
Author(s):  
Yasuhito Tanaka ◽  
Kazuaki Takahashi ◽  
Etsuro Orito ◽  
Yoshiyasu Karino ◽  
Jong-Hon Kang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Demographic History ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Nucleotide Substitutions ◽  
Genotype 4 ◽  
History Of ◽  
The Uk ◽  
Evolutionary Growth ◽  
Rna Polymerase Gene

The ancestor(s) of apparently Japan-indigenous strains of Hepatitis E virus (HEV) was probably of foreign origin, but it remains unclear when and from where it made inroads. In this study, 24 genotype 3 and 24 genotype 4 HEV strains recovered in Japan each showed a significant cluster, clearly distinct from those of foreign strains, in the phylogenetic tree constructed from an 821 nt RNA polymerase gene fragment. The evolutionary rate, approximately 0·8×10−3 nucleotide substitutions per site per year, enabled tracing of the demographic history of HEV and suggested that the ancestors of Japan-indigenous HEV had made inroads around 1900, when several kinds of Yorkshire pig were imported from the UK to Japan. Interestingly, the evolutionary growth of genotype 3 in Japan has been slow since the 1920s, whereas genotype 4 has spread rapidly since the 1980s. In conclusion, these data suggest that the indigenization and spread of HEV in Japan were associated with the popularization of eating pork.

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