Experimental infection of mongolian gerbils by a genotype 4 strain of swine hepatitis E virus

2009 ◽  
Vol 81 (9) ◽  
pp. 1591-1596 ◽  
Author(s):  
Wengui Li ◽  
Quan Sun ◽  
Ruiping She ◽  
Decheng Wang ◽  
Xinhui Duan ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Mongolian Gerbils ◽  
Genotype 4 ◽  
Swine Hepatitis

scholarly journals Synthetic Peptides Containing Three Neutralizing Epitopes of Genotype 4 Swine Hepatitis E Virus ORF2 induced Protection against Swine HEV Infection in Rabbit

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 178
Author(s):  
Yiyang Chen ◽  
Tianxiang Chen ◽  
Yuhang Luo ◽  
Jie Fan ◽  
Meimei Zhang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Keyhole Limpet Hemocyanin ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Virus Shedding ◽  
Fecal Shedding ◽  
Orf2 Protein ◽  
Swine Hepatitis ◽  
Phosphate Buffered Saline ◽  
Neutralizing Epitopes

Genotype 4 hepatitis E virus (HEV) is a zoonotic pathogen transmitted to humans through food and water. Previously, three genotype 4 swine HEV ORF2 peptides (407EPTV410, 410VKLYTS415, and 458PSRPF462) were identified as epitopes of virus-neutralizing monoclonal antibodies that partially blocked rabbit infection with swine HEV. Here, individual and tandem fused peptides were synthesized, conjugated to keyhole limpet hemocyanin (KLH), then evaluated for immunoprotection of rabbits against swine HEV infection. Forty New Zealand White rabbits were randomly assigned to eight groups; groups 1 thru 5 received three immunizations with EPTV-KLH, VKLYTS-KLH, PSRPF-KLH, EPTVKLYTS-KLH, or EPTVKLYTSPSRPF-KLH, respectively; group 6 received truncated swine HEV ORF2 protein (sp239), and group 7 received phosphate-buffered saline. After an intravenous swine HEV challenge, all group 7 rabbits exhibited viremia and fecal virus shedding by 2–4 weeks post challenge (wpc), seroconversion by 4–9 wpc, elevated alanine aminotransferase (ALT) at 2 wpc, and severe liver lymphocytic venous periphlebitis. Only 1–2 rabbits/group in groups 1–4 exhibited delayed viremia, fecal shedding, seroconversion, increased ALT levels, and slight liver lymphocytic venous periphlebitis; groups 5–6 showed no pathogenic effects. Collectively, these results demonstrate that immunization with a polypeptide containing three genotype 4 HEV ORF2 neutralizing epitopes completely protected rabbits against swine HEV infection.

Experimental infection of rabbit with swine-derived hepatitis E virus genotype 4

2019 ◽  
Vol 229 ◽  
pp. 168-175 ◽  
Author(s):  
Baoyuan Liu ◽  
Yiyang Chen ◽  
Yani Sun ◽  
Yuchen Nan ◽  
Huixia Li ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 4

scholarly journals Experimental infection of Z:ZCLA Mongolian gerbils with human hepatitis E virus

2015 ◽  
Vol 21 (3) ◽  
pp. 862 ◽  
Author(s):  
Yan Hong ◽  
Zhuo-Jing He ◽  
Wei Tao ◽  
Ting Fu ◽  
Yan-Kun Wang ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Mongolian Gerbils ◽  
Human Hepatitis

scholarly journals Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV

1998 ◽  
Vol 143 (7) ◽  
pp. 1405-1415 ◽  
Author(s):  
X.-J. Meng ◽  
P. G. Halbur ◽  
J. S. Haynes ◽  
T. S. Tsareva ◽  
J. D. Bruna ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Swine Hepatitis

Effect of swine hepatitis E virus on the livers of experimentally infected Mongolian gerbils by swine hepatitis E virus

2015 ◽  
Vol 208 ◽  
pp. 171-179 ◽  
Author(s):  
Yifei Yang ◽  
Ruihan Shi ◽  
Ruiping She ◽  
Majid Hussain Soomro ◽  
Jingjing Mao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Mongolian Gerbils ◽  
Swine Hepatitis

scholarly journals Detection of Genotype 4 Swine Hepatitis E Virus in Systemic Tissues in Cross-Species Infected Rabbits

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0171277 ◽  
Author(s):  
Qiaoxing Wu ◽  
Junqing An ◽  
Ruiping She ◽  
Ruihan Shi ◽  
Wenzhuo Hao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Swine Hepatitis
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