scholarly journals Detection and Genomic Analysis of Hepatitis E Virus Isolates from Wild Boars in Gunma Prefecture, Japan

2011 ◽  
Vol 64 (1) ◽  
pp. 67-70
Author(s):  
Taisei ISHIOKA ◽  
Chieko SAKANO ◽  
Toshie MOKUDAI ◽  
Yoko YOKOTA ◽  
Hiroyuki SAKANIWA ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Genomic Analysis ◽  
Hepatitis E ◽  
Wild Boars ◽  
Virus Isolates

scholarly journals Analysis of the full-length genome of hepatitis E virus isolates obtained from wild boars in Japan

2005 ◽  
Vol 86 (12) ◽  
pp. 3321-3326 ◽  
Author(s):  
Tsutomu Nishizawa ◽  
Masaharu Takahashi ◽  
Kazunori Endo ◽  
Shinji Fujiwara ◽  
Nobuo Sakuma ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Full Length ◽  
Partial Sequence ◽  
Genotype 3 ◽  
Entire Genome ◽  
Wild Boars ◽  
Full Length Sequence ◽  
Full Length Genome ◽  
Virus Isolates

Two (2·3 %) of 87 wild-caught boars in Japan had detectable hepatitis E virus (HEV) RNA. The two boar HEV isolates (wbJTS1 and wbJYG1) obtained in the present study and a previously reported isolate (wbJSG1) whose partial sequence had been determined were sequenced over the entire genome. The wbJSG1, wbJTS1 and wbJYG1 isolates comprised 7225 or 7226 nt, excluding the poly(A) tail, and segregated into genotype 3. They differed by 8·5–11·2 % from each other and by 8·6–18·4 % from 17 reported genotype 3 HEV isolates, including one boar isolate, in the full-length sequence. When compared with 191 reported genotype 3 HEV isolates whose partial sequences were known, these three boar isolates were closer to Japanese isolates than to isolates of non-Japanese origin (89·2±2·6 vs 85·9±2·2 %; P<0·0001). A proportion of wild boars in Japan are infected with markedly heterogeneous HEV strains that are indigenous to Japan and may serve as reservoirs of HEV.

scholarly journals Hepatitis E virus isolates of genotype 4 recovered from wild boars in the Iriomote Island, Okinawa

Kanzo ◽  
2006 ◽  
Vol 47 (3) ◽  
pp. 161-162 ◽  
Author(s):  
M Nakamura ◽  
K Taira ◽  
A Ohno ◽  
M Taira ◽  
H Sakugawa ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Wild Boars ◽  
Iriomote Island ◽  
Virus Isolates

scholarly journals Seroprevalence of Hepatitis E Virus Infection among Blood Donors in Bulgaria

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 492
Author(s):  
Magdalena Baymakova ◽  
Krasimira Terzieva ◽  
Rumen Popov ◽  
Elisaveta Grancharova ◽  
Todor Kundurzhiev ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Elisa Test ◽  
Blood Samples ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Specific Questionnaire ◽  
Positive Results

Hepatitis E virus (HEV) infection is widespread among domestic pigs, industrial swine, and wild boars in Bulgaria. The aim of the current research was to present the HEV seroprevalence among blood donors in Bulgaria. In the present study, 555 blood donors (479 males and 76 females) were enrolled from five districts in the country (Shumen, Pleven, Stara Zagora, Plovdiv, and Sofia districts). All blood samples were tested for anti-HEV IgG using the recomWell HEV IgG ELISA test (Mikrogen GmbH, Neuried, Germany). Each participating donor completed a short, structured, and specific questionnaire to document data on the current study. Anti-HEV IgG positive results were detected in 144 (25.9%) blood donors, including 129 (26.9%) males and 15 (19.7%) females. The established HEV seropositivity was 28.8% (23/80) in Shumen district, 23.2% (22/95) in Pleven district, 27.1% (38/140) in Stara Zagora district, 27.5% (44/160) in Plovdiv district, and 21.3% (17/80) in Sofia district. A high HEV seroprevalence was found for persons who declared that they were general hunters (48.7%; 19/39; p = 0.001) and hunters of wild boars (51.6%; 16/31; p = 0.001). We present the first seroprevalence rates of HEV infection in blood donors from Bulgaria. The results of our research showed high HEV seropositivity among blood donors.

scholarly journals Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany

2021 ◽  
Vol 9 (11) ◽  
pp. 2302
Author(s):  
Katja Schilling-Loeffler ◽  
Oliver Viera-Segura ◽  
Victor Max Corman ◽  
Julia Schneider ◽  
Ashish K. Gadicherla ◽  
...  
Keyword(s):  
Cell Culture ◽  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enriched Library ◽  
Genome Copy ◽  
Genotype 3 ◽  
Wild Boars ◽  
Copy Numbers ◽  
Cell Culture Isolation

Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described for human isolates so far. Here, five sera and five liver samples from HEV-RNA-positive wild boar samples were inoculated onto PLC/PRF/5 cells, incubated for 3 months and thereafter passaged for additional 6 weeks. As demonstrated by RT-qPCR, immunofluorescence and immune electron microscopy, virus was successfully isolated from two liver samples, which originally contained high HEV genome copy numbers. Both isolates showed slower growth than the culture-adapted HEV strain 47832c. In contrast to this strain, the isolated strains had no insertions in their hypervariable genome region. Next generation sequencing using an HEV sequence-enriched library enabled full genome sequencing. Strain Wb108/17 belongs to subtype 3f and strain Wb257/17 to a tentative novel subtype recently described in Italian wild boars. The results indicate that HEV can be successfully isolated in cell culture from wild boar samples containing high HEV genome copy numbers. The isolates may be used further to study the zoonotic potential of wild boar-derived HEV subtypes.

Seroprevalence of Hepatitis E Virus Infection Among Wild Boars in Western Bulgaria

2021 ◽  
Author(s):  
Ilia Tsachev ◽  
Magdalena Baymakova ◽  
Plamen Marutsov ◽  
Krasimira Gospodinova ◽  
Todor Kundurzhiev ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Wild Boars

scholarly journals Wild Boar as a Sylvatic Reservoir of Hepatitis E Virus in Poland: A Cross-Sectional Population Study

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1113
Author(s):  
Iwona Kozyra ◽  
Artur Jabłoński ◽  
Ewelina Bigoraj ◽  
Artur Rzeżutka
Keyword(s):  
Wild Boar ◽  
Regional Differences ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Igg Antibodies ◽  
Cross Sectional ◽  
Wild Boars ◽  
Wildlife Species ◽  
Faecal Samples ◽  
Molecular Assessment

The most important wildlife species in the epidemiology of hepatitis E virus (HEV) infections are wild boars, which are also the main reservoir of the virus in a sylvatic environment. The aim of the study was a serological and molecular assessment of the prevalence of HEV infections in wild boars in Poland. In total, 470 pairs of samples (wild boar blood and livers) and 433 samples of faeces were tested. An ELISA (ID.vet, France) was used for serological analysis. For the detection of HEV RNA, real-time (RT)-qPCR was employed. The presence of specific anti-HEV IgG antibodies was found in 232 (49.4%; 95%CI: 44.7–54%) sera, with regional differences observed in the seroprevalence of infections. HEV RNA was detected in 57 (12.1%, 95%CI: 9.3–15.4%) livers and in 27 (6.2%, 95%CI: 4.1–8.9%) faecal samples, with the viral load ranging from 1.4 to 1.7 × 1011 G.C./g and 38 to 9.3 × 107 G.C./mL, respectively. A correlation between serological and molecular results of testing of wild boars infected with HEV was shown. HEV infections in wild boars appeared to be common in Poland.

Detection of Hepatitis E Virus in Livers and Muscle Tissues of Wild Boars in Italy

2019 ◽  
Vol 12 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Luca De Sabato ◽  
Maria Grazia Amoroso ◽  
Giovanni Ianiro ◽  
Claudia Esposito ◽  
Luigi De Grossi ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Wild Boars ◽  
Muscle Tissues

scholarly journals Prevalence and phylogenetic analysis of hepatitis E virus in pigs, wild boars, roe deer, red deer and moose in Lithuania

2018 ◽  
Vol 60 (1) ◽  
Author(s):  
Ugne Spancerniene ◽  
Juozas Grigas ◽  
Jurate Buitkuviene ◽  
Judita Zymantiene ◽  
Vida Juozaitiene ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Hepatitis E Virus ◽  
Red Deer ◽  
Roe Deer ◽  
Hepatitis E ◽  
Wild Boars

scholarly journals Possible Foodborne Transmission of Hepatitis E Virus from Domestic Pigs and Wild Boars from Corsica

2016 ◽  
Vol 22 (12) ◽  
pp. 2197-2199 ◽  
Author(s):  
Nicole Pavio ◽  
Morgane Laval ◽  
Oscar Maestrini ◽  
François Casabianca ◽  
François Charrier ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Wild Boars ◽  
Domestic Pigs

scholarly journals A case of acute hepatitis E who was suspected to have contracted hepatitis E virus infection via consumption of dried gall bladders from wild boars

Kanzo ◽  
2016 ◽  
Vol 57 (11) ◽  
pp. 606-613 ◽  
Author(s):  
Shinichi Miyazaki ◽  
Hiroyuki Noda ◽  
Terumi Morita ◽  
Yuzuru Kai ◽  
Ayumi Osako ◽  
...  
Keyword(s):  
Virus Infection ◽  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Wild Boars ◽  
Acute Hepatitis E
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