Determination of nitrogen balance in goats fed a meal produced from hydrolyzed spent hen hard tissues

2009 ◽  
Vol 87 (3) ◽  
pp. 1068-1076 ◽  
Author(s):  
S. R. Freeman ◽  
M. H. Poore ◽  
G. B. Huntington ◽  
T. F. Middleton ◽  
P. R. Ferket
Keyword(s):  
Nitrogen Balance ◽  
Spent Hen ◽  
Hard Tissues

INFLUENCE OF OFFICE BLEACHING SYSTEMS ON THE RESISTANCE OF HARD TOOTH TISSUES

2020 ◽  
pp. 119-125
Author(s):  
Magsumova O.A. ◽  
Postnikov M.A. ◽  
Ryskina E.A. ◽  
Tkach T.M. ◽  
Polkanova V.A.
Keyword(s):  
Informed Consent ◽  
Oral Fluid ◽  
Personal Data ◽  
Acid Resistance ◽  
Non Invasive ◽  
Hard Tissues ◽  
Voluntary Informed Consent

One of the non-invasive methods for treating discoloration of hard tooth tissues is teeth whitening. The aim of this work is to assess the dynamics of changes in the acid resistance of enamel and hard tissues of teeth and the rate of its remineralization after the procedure of office teeth whitening. The study involved 123 patients aged 18 to 35 years with discoloration of various origins, with the color of hard tooth tissues on the Vita Classic A2 scale and darker. Before performing the office, teeth whitening procedure, all patients gave their written voluntary informed consent to participate in this study, as well as consent to the processing of personal data. Depending on the chosen method of office teeth whitening, patients were divided into 3 groups. The resistance of hard tooth tissues was judged based on the determination of TOER and CASRE tests. These indicators were determined at various times (5 days before the office teeth whitening procedure, 5 days after it, after 14, 30 days and 6 months). Regardless of the chosen whitening system, the office teeth whitening procedure is accompanied by a decrease in the enamel's resistance to acids and a decrease in the rate of its remineralization. The remineralizing function of oral fluid promotes the positive dynamics of the studied parameters after 14 days and after 30 days values increased due to the appointment of remineralizing therapy to all patients in 2 weeks after the teeth whitening procedure. After 6 months, all patients had high enamel resistance and the rate of its remineralization.

The micro determination of calcium in mammalian hard tissues

The Analyst ◽  
1968 ◽  
Vol 93 (1112) ◽  
pp. 722 ◽  
Author(s):  
C. Robinson ◽  
J. A. Weatherell
Keyword(s):  
Hard Tissues

scholarly journals Quantification of Bacterial Colonization in Dental Hard Tissues Using Optimized Molecular Biological Methods

2020 ◽  
Vol 11 ◽  
Author(s):  
Torsten Sterzenbach ◽  
Anne Pioch ◽  
Martin Dannemann ◽  
Christian Hannig ◽  
Marie-Theres Weber
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Hard Tissue ◽  
Bacterial Dna ◽  
Root Canals ◽  
Dental Hard Tissue ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
Biological Methods

Bacterial infections of root canals and the surrounding dental hard tissue are still a challenge due to biofilm formation as well as the complex root canal anatomy. However, current methods for analyzing biofilm formation, bacterial colonization of root canals and dental hard tissue [e.g., scanning electron microscopy, confocal laser scanning microscopy (CLSM) or determination of colony forming units (CFU)] are time-consuming and only offer a selective qualitative or semi-quantitative analysis. The aim of the present study is the establishment of optimized molecular biological methods for DNA-isolation and quantification of bacterial colonization via quantitative PCR (qPCR) from dental hard tissue. Root canals of human premolars were colonized with Enterococcus faecalis. For isolation of DNA, teeth were then grinded with a cryo mill. Since the hard tissues dentin and especially enamel belong to the hardest materials in the human organism, the isolation of bacterial DNA from root dentin is very challenging. Therefore, treatment steps for the isolation of DNA from grinded teeth were systematically analyzed to allow improved recovery of bacterial DNA from dental hard tissues. Starting with the disintegration of the peptidoglycan-layer of bacterial cells, different lysozyme solutions were tested for efficacy. Furthermore, incubation times and concentrations of chelating agents such as EDTA were optimized. These solutions are crucial for the disintegration of teeth and hence improve the accessibility of bacterial DNA. The final step was the determination of prior bacterial colonization of each root canal as determined by qPCR and comparing the results to alternative methods such as CFU. As a result of this study, optimized procedures for bacterial DNA-isolation from teeth were established, which result in an increased recovery rate of bacterial DNA. This method allows a non-selective and straightforward procedure to quantify bacterial colonization from dental hard tissue. It can be easily adapted for other study types such as microbiome studies and for comparable tissues like bones.

DETERMINATION OF NITROGEN BALANCE OF INFANTS LESS THAN 6 MONTHS OF AGE

PEDIATRICS ◽  
1958 ◽  
Vol 22 (1) ◽  
pp. 94-100
Author(s):  
Samuel J. Fomon ◽  
Lora N. Thomas ◽  
Robert L. Jensen ◽  
Charles D. May
Keyword(s):  
Nitrogen Balance ◽  
Metabolic Balance

The procedure employed by the authors for the performance of metabolic balance studies is reported in detail. Data concerning the retention of nitrogen by infants during consecutively performed 3-day metabolic balance studies are presented.

THE DETERMINATION OF THE NITROGEN BALANCE INDEX IN NORMAL AND HYPOPROTEINEMIC DOGS

1946 ◽  
Vol 47 (3) ◽  
pp. 245-271 ◽  
Author(s):  
JAMES B. ALLISON ◽  
JOHN A. ANDERSON ◽  
ROBERT D. SEELEY
Keyword(s):  
Nitrogen Balance ◽  
Balance Index

scholarly journals THE DETERMINATION OF NITROGEN BALANCE INDEXES OF PROTEIN HYDROLYSATES IN DOGS

1947 ◽  
Vol 171 (1) ◽  
pp. 91-100
Author(s):  
James B. Allison ◽  
Robert D. Seeley ◽  
Frederick P. Ferguson
Keyword(s):  
Nitrogen Balance ◽  
Protein Hydrolysates

scholarly journals TOOTH HARD TISSUES DISEASES OF ATHLETES: TREATMENT AND PREVENTION FEATURES

2020 ◽  
Vol 16 (2) ◽  
pp. 37-46
Author(s):  
Yuliya Mandra ◽  
Anastasiya Kotikova ◽  
Elena Svetlakova ◽  
Elena Semencova ◽  
N. Gegalina ◽  
...  
Keyword(s):  
Complex Treatment ◽  
Main Research ◽  
Filling Materials ◽  
Treatment And Prevention ◽  
Hard Tissues ◽  
Dental Diseases ◽  
Impaired Health ◽  
New Treatment

Thing. In recent years, the importance of sports in improving the health of the population by involving people in physical education has increased. However, sports of the highest achievements are associated with the risk of occupational diseases. Physical and emotional stress of athletes can lead to impaired health. Dental diseases are also common in people involved in sports. In domestic and foreign literature, athletes describe bruxism, occlusal disorders, periodontal disease, tooth abrasion. Of particular interest are injuries of hard tissues of the teeth of athletes in connection with their highest prevalence, ineffectiveness of treatment and prevention. The goal is on the basis of a clinical and laboratory study of the state of hard tooth tissues in athletes, to develop and justify the tactics of complex treatment, prevention of diseases of hard tooth tissues. Methodology. All participants underwent a comprehensive dental examination, which included the main research methods (determination of complaints, medical history, external examination, examination of the oral cavity, detection of diseases of hard tissues of the teeth, malocclusion) and additional (X-ray examination, “hamburg testing”, diagnosis of T-scan, assessment of oral fluid indices, cytological examination of buccal epithelium, electrometry of hard tissues of teeth, assessment of resistance of hard tissues of teeth). Results. Based on the results, we developed a new treatment-and-prophylactic toothpaste based on Silivatit organosilicon glycerogel hydrogel with the addition of nanostructured hydroxyapatite. Its biocompatibility, remineralizing and anti-inflammatory activity are proved, and clinical efficacy in athletes with diseases of hard tissues of the teeth is confirmed. The tactics of the complex treatment and prevention of diseases of hard tooth tissues in athletes based on the differentiated choice of filling materials have been substantiated. Conclusions. Thus, the optimized tactics of complex treatment and prevention of diseases of hard tissues of the teeth prevents the progression of diseases, the development of complications, and improves the quality of life of athletes.

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