The micro determination of calcium in mammalian hard tissues

The Analyst ◽  
1968 ◽  
Vol 93 (1112) ◽  
pp. 722 ◽  
Author(s):  
C. Robinson ◽  
J. A. Weatherell
Keyword(s):  
Hard Tissues

INFLUENCE OF OFFICE BLEACHING SYSTEMS ON THE RESISTANCE OF HARD TOOTH TISSUES

2020 ◽  
pp. 119-125
Author(s):  
Magsumova O.A. ◽  
Postnikov M.A. ◽  
Ryskina E.A. ◽  
Tkach T.M. ◽  
Polkanova V.A.
Keyword(s):  
Informed Consent ◽  
Oral Fluid ◽  
Personal Data ◽  
Acid Resistance ◽  
Non Invasive ◽  
Hard Tissues ◽  
Voluntary Informed Consent

One of the non-invasive methods for treating discoloration of hard tooth tissues is teeth whitening. The aim of this work is to assess the dynamics of changes in the acid resistance of enamel and hard tissues of teeth and the rate of its remineralization after the procedure of office teeth whitening. The study involved 123 patients aged 18 to 35 years with discoloration of various origins, with the color of hard tooth tissues on the Vita Classic A2 scale and darker. Before performing the office, teeth whitening procedure, all patients gave their written voluntary informed consent to participate in this study, as well as consent to the processing of personal data. Depending on the chosen method of office teeth whitening, patients were divided into 3 groups. The resistance of hard tooth tissues was judged based on the determination of TOER and CASRE tests. These indicators were determined at various times (5 days before the office teeth whitening procedure, 5 days after it, after 14, 30 days and 6 months). Regardless of the chosen whitening system, the office teeth whitening procedure is accompanied by a decrease in the enamel's resistance to acids and a decrease in the rate of its remineralization. The remineralizing function of oral fluid promotes the positive dynamics of the studied parameters after 14 days and after 30 days values increased due to the appointment of remineralizing therapy to all patients in 2 weeks after the teeth whitening procedure. After 6 months, all patients had high enamel resistance and the rate of its remineralization.

scholarly journals Quantification of Bacterial Colonization in Dental Hard Tissues Using Optimized Molecular Biological Methods

2020 ◽  
Vol 11 ◽  
Author(s):  
Torsten Sterzenbach ◽  
Anne Pioch ◽  
Martin Dannemann ◽  
Christian Hannig ◽  
Marie-Theres Weber
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Hard Tissue ◽  
Bacterial Dna ◽  
Root Canals ◽  
Dental Hard Tissue ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
Biological Methods

Bacterial infections of root canals and the surrounding dental hard tissue are still a challenge due to biofilm formation as well as the complex root canal anatomy. However, current methods for analyzing biofilm formation, bacterial colonization of root canals and dental hard tissue [e.g., scanning electron microscopy, confocal laser scanning microscopy (CLSM) or determination of colony forming units (CFU)] are time-consuming and only offer a selective qualitative or semi-quantitative analysis. The aim of the present study is the establishment of optimized molecular biological methods for DNA-isolation and quantification of bacterial colonization via quantitative PCR (qPCR) from dental hard tissue. Root canals of human premolars were colonized with Enterococcus faecalis. For isolation of DNA, teeth were then grinded with a cryo mill. Since the hard tissues dentin and especially enamel belong to the hardest materials in the human organism, the isolation of bacterial DNA from root dentin is very challenging. Therefore, treatment steps for the isolation of DNA from grinded teeth were systematically analyzed to allow improved recovery of bacterial DNA from dental hard tissues. Starting with the disintegration of the peptidoglycan-layer of bacterial cells, different lysozyme solutions were tested for efficacy. Furthermore, incubation times and concentrations of chelating agents such as EDTA were optimized. These solutions are crucial for the disintegration of teeth and hence improve the accessibility of bacterial DNA. The final step was the determination of prior bacterial colonization of each root canal as determined by qPCR and comparing the results to alternative methods such as CFU. As a result of this study, optimized procedures for bacterial DNA-isolation from teeth were established, which result in an increased recovery rate of bacterial DNA. This method allows a non-selective and straightforward procedure to quantify bacterial colonization from dental hard tissue. It can be easily adapted for other study types such as microbiome studies and for comparable tissues like bones.

scholarly journals TOOTH HARD TISSUES DISEASES OF ATHLETES: TREATMENT AND PREVENTION FEATURES

2020 ◽  
Vol 16 (2) ◽  
pp. 37-46
Author(s):  
Yuliya Mandra ◽  
Anastasiya Kotikova ◽  
Elena Svetlakova ◽  
Elena Semencova ◽  
N. Gegalina ◽  
...  
Keyword(s):  
Complex Treatment ◽  
Main Research ◽  
Filling Materials ◽  
Treatment And Prevention ◽  
Hard Tissues ◽  
Dental Diseases ◽  
Impaired Health ◽  
New Treatment

Thing. In recent years, the importance of sports in improving the health of the population by involving people in physical education has increased. However, sports of the highest achievements are associated with the risk of occupational diseases. Physical and emotional stress of athletes can lead to impaired health. Dental diseases are also common in people involved in sports. In domestic and foreign literature, athletes describe bruxism, occlusal disorders, periodontal disease, tooth abrasion. Of particular interest are injuries of hard tissues of the teeth of athletes in connection with their highest prevalence, ineffectiveness of treatment and prevention. The goal is on the basis of a clinical and laboratory study of the state of hard tooth tissues in athletes, to develop and justify the tactics of complex treatment, prevention of diseases of hard tooth tissues. Methodology. All participants underwent a comprehensive dental examination, which included the main research methods (determination of complaints, medical history, external examination, examination of the oral cavity, detection of diseases of hard tissues of the teeth, malocclusion) and additional (X-ray examination, “hamburg testing”, diagnosis of T-scan, assessment of oral fluid indices, cytological examination of buccal epithelium, electrometry of hard tissues of teeth, assessment of resistance of hard tissues of teeth). Results. Based on the results, we developed a new treatment-and-prophylactic toothpaste based on Silivatit organosilicon glycerogel hydrogel with the addition of nanostructured hydroxyapatite. Its biocompatibility, remineralizing and anti-inflammatory activity are proved, and clinical efficacy in athletes with diseases of hard tissues of the teeth is confirmed. The tactics of the complex treatment and prevention of diseases of hard tooth tissues in athletes based on the differentiated choice of filling materials have been substantiated. Conclusions. Thus, the optimized tactics of complex treatment and prevention of diseases of hard tissues of the teeth prevents the progression of diseases, the development of complications, and improves the quality of life of athletes.

Determination of ablation threshold of dental hard tissues irradiated with Er:YAG and Er,Cr:YSGG lasers

2013 ◽  
Vol 11 (5) ◽  
pp. 051701-51705
Author(s):  
Zhenlin Zhan Zhenlin Zhan ◽  
Xianzeng Zhang Xianzeng Zhang ◽  
Wenqing Guo Wenqing Guo ◽  
Shusen Xie Shusen Xie
Keyword(s):  
Ablation Threshold ◽  
Dental Hard Tissues ◽  
Hard Tissues

scholarly journals FACTORS AFFECTING THE DENTAL STATUS OF THE RUSSIAN POPULATION

2020 ◽  
Vol 16 (1) ◽  
pp. 47-57
Author(s):  
Elena Silagadze ◽  
Albert Salahov ◽  
Said Ksembaev ◽  
Rustem Bykeev
Keyword(s):  
Risk Factors ◽  
Preventive Measures ◽  
Periodontal Diseases ◽  
Dental Health ◽  
Dental Status ◽  
Actual Problem ◽  
Factors Affecting ◽  
Hard Tissues ◽  
Quantitative Characteristics

Subject. Tooth decay is still among the most common diseases. Determination of risk factors, their quantitative characteristics allow individualizing preventive measures depending on the socio-medical, climatic and other conditions of a particular region. A literature review is devoted to the actual problem of dentistry - factors affecting the development of dental caries and periodontal diseases. The purpose of the study is to analyze literature to study the factors affecting the state of the dental status of the population of Russia. Methodology. A review of literature sources was carried out and factors affecting the status of dental status were analyzed. Results. Risk factors affecting the degree of caries resistance of tooth hard tissues and determining their stability were divided into: providing local anti-carious protection of the teeth; creating an indirect general anti-carious protection of the teeth at the level of the human body as a whole; increasing the degree of risk in the development and course of diseases and/or reducing the degree of protective properties of hard tissues of the tooth; indirectly, through external systems in relation to a given organism as a system, ensuring the likelihood of developing a caries morbidity. Conclusions. Determination of risk factors, their quantitative characteristics allows individualizing preventive measures depending on a set of factors. Literature data confirm that timely visits to the dentist for preventive purposes, the implementation of the doctor’s recommendations for individual preventive measures, reducing the frequency of food intake, which include refined and “sticky” sugar-containing components, balancing the diet will help maintain dental health.

Determination of nitrogen balance in goats fed a meal produced from hydrolyzed spent hen hard tissues

2009 ◽  
Vol 87 (3) ◽  
pp. 1068-1076 ◽  
Author(s):  
S. R. Freeman ◽  
M. H. Poore ◽  
G. B. Huntington ◽  
T. F. Middleton ◽  
P. R. Ferket
Keyword(s):  
Nitrogen Balance ◽  
Spent Hen ◽  
Hard Tissues

scholarly journals АНАЛІЗ СТОМАТОЛОГІЧНОГО СТАТУСУ ШКОЛЯРІВ МІСТА СТРИЯ ЗАЛЕЖНО ВІД РЕЖИМУ ТА РАЦІОНУ ХАРЧУВАННЯ

2018 ◽  
Author(s):  
O. I. Lebid ◽  
K. M. Duda ◽  
H. V. Stoikevych
Keyword(s):  
Oral Hygiene ◽  
Metabolic Disorders ◽  
Dental Health ◽  
Statistical Processing ◽  
Dental Examination ◽  
Hard Tissues ◽  
Definition Of ◽  
Somatic Diseases ◽  
The City

The analysis of the stomatological status of schoolchildren in the city of Stryi shows the influence of the regime and diet, physical activity of students in conjunction with other factors, to metabolic disorders that are trigger in the development of generalsomatic diseases and diseases of hard tissues of teeth.The aim of the study – to analyze the results of the questionnaire and dental examination, on the effect of diet on the stomatological status of schoolchildren in the city of Stryi.Materials and Methods. 96 children aged 14–16 participated in the studies. Among the schoolchildren in the city of Stryi, Lviv oblast, a sociological survey on diet and motor activity was conducted by questionnaire on the poll developed by us. The obtained data were compared with the results of the dental examination, which included the determination of the intensity of caries of the teeth according to the CPI index. The examination of oral hygiene was carried out using the Green-Vermillion (OH I-S) Hygiene Index. The results were summarized into a common table to facilitate statistical processing of information.Results and Discussion. As a result of our research, we have established a clear link between the diet and the diet of schoolchildren on their dental health. So, we found that in the diet of schoolchildren decreased the consumption of dairy products, fish and meat, and on the contrary increased the use of "fast foods", sweets, sweet beverages. Our definition of dental status has shown that the prevalence of caries is 93.69 % for schoolchildren who do not adhere to the regime and diet, and only 6.31 % of the surveyed have intact dental rows.Conclusions. The according to the results of our research, it can be concluded that inappropriate nutrition, violation of its mode and structure, sedentary lifestyle, lack of oral hygiene, in our opinion, together with other factors, lead to metabolic disorders that are trigger in the development of general-somatic diseases, and diseases of hard tissues of teeth.

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