Effects of sodium hyaluronate and methylprednisolone acetate on proteoglycan synthesis in equine articular cartilage explants

2005 ◽  
Vol 66 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Aimie J. Doyle ◽  
Allison A. Stewart ◽  
Peter D. Constable ◽  
Jo Ann C. Eurell ◽  
David E. Freeman ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Sodium Hyaluronate ◽  
Cartilage Explants ◽  
Proteoglycan Synthesis ◽  
Methylprednisolone Acetate

The Effect of Total Meniscectomy versus Caudal Pole Hemimeniscectomy on the Stifle Joint of the Sheep

1999 ◽  
Vol 12 (02) ◽  
pp. 56-63 ◽  
Author(s):  
C. R. Bellenger ◽  
P. Ghosh ◽  
Y. Numata ◽  
C. Little ◽  
D. S. Simpson
Keyword(s):  
Tissue Culture ◽  
Articular Cartilage ◽  
Fibrous Tissue ◽  
Cartilage Degeneration ◽  
Medial Compartment ◽  
Proteoglycan Synthesis ◽  
Stifle Joint ◽  
Medial Meniscectomy ◽  
Tibial Condyle ◽  
Articular Cartilage Degeneration

SummaryTotal medial meniscectomy and caudal pole hemimeniscectomy were performed on the stifle joints of twelve sheep. The two forms of meniscectomy produced a comparable degree of postoperative lameness that resolved within two weeks of the operations. After six months the sheep were euthanatised and the stifle joints examined. Fibrous tissue that replaced the excised meniscus in the total meniscectomy group did not cover as much of the medial tibial condyle as the residual cranial pole and caudal fibrous tissue observed following hemimeniscectomy. The articular cartilage from different regions within the joints was examined for gross and histological evidence of degeneration. Analyses of the articular cartilage for water content, glycosaminoglycan composition and DNA content were performed. The proteoglycan synthesis and release from explanted articular cartilage samples in tissue culture were also measured. There were significant pathological changes in the medial compartment of all meniscectomised joints. The degree of articular cartilage degeneration that was observed following total meniscectomy and caudal pole meniscectomy was similar. Caudal pole hemimeniscectomy, involving transection of the meniscus, causes the same degree of degeneration of the stifle joint that occurs following total meniscectomy.The effect of total medial meniscectomy versus caudal pole hemimeniscectomy on the stifle joint of sheep was studied experimentally. Six months after the operations gross pathology, histopathology, cartilage biochemical analysis and the rate of proteoglycan synthesis in tissue culture were used to compare the articular cartilage harvested from the meniscectomised joints. Degeneration of the articular cartilage from the medial compartment of the joints was present in both of the groups. Caudal pole hemimeniscectomy induces a comparable degree of articular cartilage degeneration to total medial meniscectomy in the sheep stifle joint.

Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants

1998 ◽  
Vol 111 (5) ◽  
pp. 573-583
Author(s):  
T.M. Quinn ◽  
A.J. Grodzinsky ◽  
M.D. Buschmann ◽  
Y.J. Kim ◽  
E.B. Hunziker
Keyword(s):  
Morphological Changes ◽  
Dynamic Compression ◽  
Compressive Strain ◽  
Cell Length ◽  
Cartilage Explants ◽  
Proteoglycan Synthesis ◽  
Length Scale ◽  
Mechanical Compression ◽  
Static Compression ◽  
Associated Matrix

We have used new techniques of cell-length scale quantitative autoradiography to assess matrix synthesis, deposition, and deformation around individual chondrocytes in mechanically compressed cartilage explants. Our objectives were to: (1) quantify the effects of static and dynamic compression on the deposition of newly synthesized proteoglycans into cell-associated and further-removed matrices; (2) measure cell-length scale matrix strains and morphological changes of the cell and matrix associated with tissue compression; and (3) relate microscopic physical stimuli to changes in proteoglycan synthesis as functions of compression level and position within mechanically compressed explants. Results indicate a high degree of structural organization in the extracellular matrix, with the pericellular matrix associated with the most rapid rates of proteoglycan deposition, and greatest sensitivity to mechanical compression. Static compression could stimulate directional deposition of secreted proteoglycans around chondrocytes, superimposed on an inhibition of proteoglycan synthesis; these events followed trends for compressive strain in the cell-associated matrix. Conversely, proteoglycan synthesis and pericellular deposition was stimulated by dynamic compression. Results suggest that cell-matrix interactions in the cell-associated matrix may be a particularly important aspect of the chondrocyte response to mechanical compression, possibly involving macromolecular transport limitations and morphological changes associated with fluid flow and local compaction of the matrix around cells.

scholarly journals Reduction of interleukin‐17–induced inhibition of chondrocyte proteoglycan synthesis in intact murine articular cartilage by interleukin‐4

2000 ◽  
Vol 43 (6) ◽  
pp. 1300-1306 ◽  
Author(s):  
Erik Lubberts ◽  
Leo A. B. Joosten ◽  
Fons A. J. Van De Loo ◽  
Liduine A. M. Van Den Bersselaar ◽  
Wim B. Van Den Berg
Keyword(s):  
Articular Cartilage ◽  
Interleukin 4 ◽  
Proteoglycan Synthesis ◽  
Interleukin 17
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