Effects of antirheumatic drugs on the interleukin-1α induced synthesis and activation of proteinases in articular cartilage explants in culture

1989 ◽  
Vol 27 (3-4) ◽  
pp. 261-264 ◽  
Author(s):  
C. Arsenis ◽  
J. McDonnell
Keyword(s):  
Articular Cartilage ◽  
Cartilage Explants ◽  
Antirheumatic Drugs ◽  
Interleukin 1Α

scholarly journals Modulation of aggrecan and link-protein synthesis in articular cartilage

1992 ◽  
Vol 288 (3) ◽  
pp. 721-726 ◽  
Author(s):  
A J Curtis ◽  
R J Devenish ◽  
C J Handley
Keyword(s):  
Articular Cartilage ◽  
Half Life ◽  
Core Protein ◽  
Calf Serum ◽  
Cartilage Explants ◽  
Dependent Manner ◽  
Link Protein ◽  
The Core ◽  
Explant Cultures ◽  
Igf I

The addition of serum or insulin-like growth factor-I (IGF-I) to the medium of explant cultures of bovine articular cartilage is known to stimulate the synthesis of aggrecan in a dose-dependent manner. The half-life of the pool of proteoglycan core protein was measured in adult articular cartilage cultured for 6 days in the presence and absence of 20 ng of IGF-I/ml and shown to be 24 min under both sets of conditions. The half-life of the mRNA pool coding for aggrecan was also determined and shown to be approx. 4 h in cartilage maintained in culture with or without IGF-I. The pool size of mRNA coding for aggrecan core protein increased 5-6-fold in cartilage explants maintained in culture in medium containing 20% (v/v) fetal-calf serum; however, in tissue maintained with medium containing IGF-I there was no increase in the cellular levels of this mRNA. This suggests that aggrecan synthesis is stimulated by IGF-I at the level of translation of mRNA coding for the core protein of this proteoglycan and that other growth factors are present in serum that stimulate aggrecan synthesis at the level of transcription of the core-protein gene. Inclusion of serum or IGF-I in the medium of cartilage explant cultures induced increases in the amounts of mRNA coding for type II collagen and link protein, whereas only serum enhanced the amount of mRNA for the core protein of decorin.

Stromelysin Activity in Canine Cranial Cruciate Ligament Rupture

1999 ◽  
Vol 12 (04) ◽  
pp. 159-165 ◽  
Author(s):  
Nadja Sigrist ◽  
A. Busato ◽  
Brigitte von Rechenberg ◽  
P. Schawalder ◽  
D. Spreng
Keyword(s):  
Articular Cartilage ◽  
Synovial Membrane ◽  
Cruciate Ligament ◽  
Cartilage Explants ◽  
Control Groups ◽  
Cranial Cruciate Ligament ◽  
Ligament Rupture ◽  
Cruciate Ligament Rupture ◽  
Explant Cultures ◽  
Cranial Cruciate Ligament Rupture

SummaryThe goal of our study was to compare values of stromelysin activity in stifle joint tissues, from dogs with osteoarthritis, secondary to naturally acquired cranial cruciate ligament (CCL) rupture and from a control population.Twenty four dogs (CCL group) with osteoarthritis (OA), secondary to CCL rupture, were evaluated. The control groups consisted of 22 beagles (control #1) and 14 dogs (control #2) without CCL rupture. Articular cartilage, synovial membrane and CCL tissue specimens were harvested during operations in the CCL group and immediately following euthanasia in the control groups. The specimens were submitted for routine histology and for explant tissue cultures.Stromelysin activity was measured in the supernatant of explanted cultures. The results of stromelysin concentrations were reported as mean ± STD and compared to histological cartilage degeneration, synovial membrane inflammation and ligament changes. Stromelysin activity in cartilage explants of the CCL group (70 ± 82.5 U/g) was significantly higher when compared to the control #1 (4.2 ± 6.3 U/g) and control #2 (15 ±10 U/g) groups. The synovial membrane explant cultures of the CCL group produced less stromelysin compared to the control group. Whereas ligament cultures showed a tendency toward higher activity of stromelysin when compared to the control groups. An association between the severity of histological OA changes in the cartilage and stromelysin activity in cartilage explants was demonstrated. We conclude that dogs with OA, secondary to naturally acquired CCL rupture, release higher stromelysin levels in articular cartilage and cranial cruciate ligament explant cultures when compared to the controls. Our results indicate that stromelysin production in articular cartilage is related to the severity of OA.Stromelysin, one of the major metalloproteinases degrades articular cartilage mainly by cleavage of proteoglycans. Increased levels of stromelysin could be demonstrated in cartilage of stifle joints from dogs with naturally acquired cranial cruciate ligament rupture using a radioimmunoassay. There was an indication in this study that the activity of stromelysin is associated with the severity of osteoarthritic changes.Presented in part at the European College of Veterinary Surgeons Annual Scientific Meeting, Pörtschach-Austria, June 1998.

Lysosomal Molecules are Up-Regulated in the Articular Cartilage Explants Subjected to Oxidative Stress and in the Cartilage from an Osteoarthritis-Induced Rat Model

2012 ◽  
Vol 3 (1) ◽  
pp. 72-78
Author(s):  
Guadalupe Jimenez-Carbajal ◽  
Magdalena Miranda-Sanchez ◽  
Raymundo Cruz ◽  
Maylin Almonte- Becerril ◽  
Carlos Lavalle-Montalvo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Articular Cartilage ◽  
Rat Model ◽  
Cartilage Explants

Effects of Electromagnetic Fields on Proteoglycan Metabolism of Bovine Articular Cartilage Explants

2003 ◽  
Vol 44 (3-4) ◽  
pp. 154-159 ◽  
Author(s):  
Monica De Mattei ◽  
Michela Pasello ◽  
Agnese Pellati ◽  
Giordano Stabellini ◽  
Leo Massari ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Electromagnetic Fields ◽  
Cartilage Explants ◽  
Bovine Articular Cartilage

Ameliorating Glycosaminoglycan (GAG) Loss and Cell Death in Articular Cartilage Following Single-Impact Loading

2007 ◽  
Author(s):  
Roman M. Natoli ◽  
Kyriacos A. Athanasiou
Keyword(s):  
Extracellular Matrix ◽  
Cell Death ◽  
Articular Cartilage ◽  
Impact Loading ◽  
Biomechanical Properties ◽  
Cartilage Explants ◽  
Single Impact ◽  
Igf I ◽  
Post Traumatic ◽  
Bioactive Agents

Impact loading of articular cartilage leads to post-traumatic osteoarthritis (OA) through its effects on the cells and extracellular matrix (ECM) of the tissue. Studies have shown the level of impact or injurious compression correlates with increased cell death, degradation of the ECM, and detrimental changes in biomechanical properties [1]. Recently, several bioactive agents, such as P188 and IGF-I, have shown promising results by reducing cell death following injurious compression of cartilage explants [2, 3].

Identification of Distinct Metabolic Pools of Aggrecan and Their Relationship to Type VI Collagen in the Chondrons of Mature Bovine Articular Cartilage Explants

1998 ◽  
Vol 37 (3-4) ◽  
pp. 277-293 ◽  
Author(s):  
Gavin m. Winter ◽  
C. Anthony Poole ◽  
Mirna Z. Ilic ◽  
Jacqueline M. Ross ◽  
H. Clem Robinson ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Explants ◽  
Type Vi Collagen ◽  
Bovine Articular Cartilage

Influence of Stress Magnitude on Water Loss and Chondrocyte Viability in Impacted Articular Cartilage

2003 ◽  
Vol 125 (5) ◽  
pp. 594-601 ◽  
Author(s):  
Dejan Milentijevic ◽  
David L. Helfet ◽  
Peter A. Torzilli
Keyword(s):  
Articular Cartilage ◽  
Water Loss ◽  
Articular Surface ◽  
Axial Strain ◽  
Biological Response ◽  
Peak Stress ◽  
Cartilage Explants ◽  
Axial Deformation ◽  
Chondrocyte Viability ◽  
Chondrocyte Death

The objective of this study was to assess mechano-biological response of articular cartilage when subjected to a single impact stress. Mature bovine cartilage explants were impacted with peak stresses ranging from 10 to 60 MPa at a stress rate of 350 MPa/s. Water loss, matrix axial deformation, dynamic impact modulus (DIM), and cell viability were measured immediately after impaction. The water loss through the articular surface (AS) was small and ranged from 1% to 6% with increasing peak stress. The corresponding axial strains ranged from 2.5% to 25%, respectively, while the DIM was 455.9±111.9 MPa. Chondrocyte death started at the articular surface and increased in depth to a maximum of 6% (70 μm) of the cartilage thickness at the highest stress. We found that the volumetric (axial) strain was more than twice the amount of water loss at the highest peak stress. Furthermore, specimens impacted such that the interstitial water was forced through the deep zone (DZ) had less water loss, a higher DIM, and no cell death. These findings appear to be due to matrix compaction in the superficial region causing higher compressive strains to occur at the surface rather than in the deeper zones.

Sign in / Sign up

Export Citation Format

Share Document