scholarly journals Extraction of leaf protein from green crops. Chemical composition and nutritive value of products of fractionation 

1983 ◽  
Vol 55 (2) ◽  
pp. 143-154
Author(s):  
Matti Näsi
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Soluble Sugars ◽  
Protein Solubility ◽  
Leaf Protein ◽  
In Vitro Digestibility ◽  
Growth Stages ◽  
Crude Fibre ◽  
True Protein

Leaf protein was extracted from different green crops in 11 pilot plant esperiments. Of the crops, 4 were grass, 6 clover and one pea. The extraction of juice was on average 55 % of the fresh weight of the green crop and the values for dry matter (DM) and crude protein (CP) were 22.6 and 24.1 %. Clover gave better recoveries of protein than grass. In the leaf protein concentrate (LPC) obtained from the juice, the separation ratios for DM, CP and TP (true protein) were, respectively, 23.7 %, 48.0 and 80.7 %. Heating to 85°C gave more efficient recoveries of LPC than the combination of heating and acid precipitation. The average DM content of the pressed pulp was 30.4 %, the corresponding value for the whole crop being 16.5 %. Measured on a DM basis, the CP content of the pressed pulp was only 0.4 % units lower than in untreated forage, but the crude fibre content was 7.3 % units higher. In vitro organic matter digestibility and the pepsin-HCI solubility of crude protein were on average 5.1 and 5.5 % units lower in the pulp. The average DM of the plant juice was 6.5 % and contained 21.9 % ash, 21.5 % CP, 10.7 % TP and 29.9 % soluble sugars. Clover and pea had much higher values for CP and TP than grass. In the LPC preparations, CP and TP averaged 43.6 % and 38.5 % of DM. Heat treatment gave higher protein content than precipitation of LPC by combined heating and acidification. The in vitro digestibility and protein solubility of LPC were high, on average 85.6 % and 80.2 %. LPC had fairly high contents of lysine and methionine, 4.1 % and 1.6 g/16 g N. There were only small differences in the amino acid composition between grass and clover and between crops harvested at different growth stages. Green crop fractionation is a potential means of improving grassland production and utilization. Promising results have been obtained with plant juice and LPC fed to monogastric animals and pressed pulp residues in diets for ruminants. The economic aspects of fractionation remain to be evaluated.

scholarly journals Leaf protein production from energy willow leaves

1983 ◽  
Vol 55 (2) ◽  
pp. 155-162
Author(s):  
Matti Näsi
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Protein Extraction ◽  
Leaf Protein ◽  
In Vitro Digestibility ◽  
Leaf Protein Concentrate ◽  
The Mean ◽  
True Protein ◽  
Leaf Juice

The extraction of protein from leaves of two energy willow clones (Salic cv. Aquatica and S. viminalis) and the chemical composition and nutritive value of the leaf fractions were investigated in six experiments. The preservation of leaf juice and wet leaf protein concentrate (LPC) was also studied. The extraction of juice averaged 33 % of the fresh weight, and the extraction ratios of dry matter (DM) and crude protein (CP) were 10.5 % and 2.9 %. The percentages of juice DM, CP and TP (true protein) separated in LPC were 6 %, 21 % and 48 %, respectively. The separation values were higher when LPC was precipitated by heating than by acidification. Removal of nutrients from the leaves was low and there were only minor differences in composition between the pressed pulp and the leaves. The leaf juice contained 8.3 % DM, of which 20.9 % was ash, 4.9 % CP, 1.9 % TP and 43.2 % sugars. The LPC had an average DM content of 19.6 %, CP being 21.4 % and TP 19.5 % of DM. The in vitro digestibility and pepsin-HCI-solubility of the protein were low. The mean tannin content of the LPC averaged 3.6 % of DM. The mean lysine and methionine contents of the LPC were low, 3.3 and 1.5 g/16 g N, respectively. The preservation of the leaf juice was satisfactory even without addition of preservative. Preservation of wet LPC was good with additives used at the 1.0 % v/w level. Leaf protein extraction was low and the nutritive value of LPC was not particularly high. This may have been partly due to the late date of harvesting and to the fact that the leaves were frozen for storage.

CHEMICAL COMPOSITION AND IN VITRO DIGESTIBILITY OF RANGE FORAGE PLANTS OF THE STIPA-BOUTELOUA PRAIRIE

1967 ◽  
Vol 47 (2) ◽  
pp. 161-167 ◽  
Author(s):  
S. Smoliak ◽  
L. M. Bezeau
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Calcium Content ◽  
Early Spring ◽  
In Vitro Digestibility ◽  
Native Grasses ◽  
Stages Of Growth ◽  
Artemisia Species ◽  
Shrubby Species

Five native grasses and one sedge of the Stipa-Bouteloua prairie, three introduced grasses collected at five stages of growth, and four shrubby species collected at three stages of growth were analyzed for proximate chemical constituents.The amounts of phosphorus and digestible and crude protein of all species decreased with maturity, while the cellulose increased. Introduced grasses generally contained more crude protein than native grasses. Shrubby species were higher in crude protein and phosphorus than native grasses. No consistent trend was observed in relative total ash and calcium content at progressive stages of development. The estimated nutritive value index was high for all grasses and low for two Artemisia species. The seasonal declines in crude protein and phosphorus suggest that protein and phosphorus supplements are desirable for range cattle during the fall, winter, and early spring.

Selection for crude protein content in Phalaris tuberosa L. I. Response to selection and preliminary studies on correlated responses

1969 ◽  
Vol 20 (4) ◽  
pp. 643 ◽  
Author(s):  
RJ Clements
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Breeding Population ◽  
In Vitro Digestibility ◽  
Seedling Vigour ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Selection For ◽  
Pasture Plant

In a highly variable breeding population of P. tuberosa, marked responses were obtained to three generations of selection for high and low crude protein concentration (percentage nitrogen x 6.25) in whole tillers at heading. Total response was similar in each direction, and realized heritability estimates were h2 = 0.25 and h2 = 0.20 in the high and low directions respectively. The responses were accompanied by positively correlated changes in in vitro digestibility and in characters commonly used as indicators of nutritive value of herbage. However, there were large negatively correlated changes in seedling vigour, relative growth rates, and other morphological and physiological characters. The implications of the results for pasture plant breeding are discussed.

GENETIC VARIATION FOR FORAGE QUALITY OF ALFALFA STEMS

1987 ◽  
Vol 67 (4) ◽  
pp. 1057-1067 ◽  
Author(s):  
D. R. BUXTON ◽  
G. C. MARTEN ◽  
J. S. HORNSTEIN
Keyword(s):  
Cell Wall ◽  
Genetic Variation ◽  
Medicago Sativa ◽  
Crude Protein ◽  
Nutritive Value ◽  
Growth Traits ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Medicago Sativa L

When normally harvested for hay, the basal portion of alfalfa (Medicago sativa L.) stems is low in forage quality. This study was conducted to determine whether genetic variation for in vitro digestible dry matter (IVDDM) and crude protein (CP) concentration of stems of perennial Medicago spp. could be identified and whether morphological and chemical traits were associated with variation in these characteristics of stems. The lower six nodes and internodes of stems and total herbage from 64 Plant Introduction (PI) accessions and five cultivars of field-grown plants were harvested twice when most plants were at the early seed-pod stage of maturity. Several morphological and growth traits were measured, and both stems and total herbage were evaluated for IVDDM and CP concentration. Covariant analysis was used to adjust in IVDDM and CP concentration of stems for differences in plant maturity. Significant variation for IVDDM and CP concentration in stems existed among the cultivars and among the accessions. Some accessions had significantly higher stem IVDDM or CP concentration than control cultivars. The M. sativa accession, PI 440496, had the fourth highest stem IVDDM and third highest stem CP, with concentrations of 374 and 79 g kg−1, respectively. The number of ridges on stems (stem shape) was significantly correlated with stem IVDDM and CP concentration with average correlation coefficients of 0.30 and 0.35, respectively. Cell-wall concentration and lignin concentration per unit cell wall of stems were both negatively correlated with stem IVDDM and CP concentration. Selection for improved nutritive value of stems may have potential for improving the nutritive value of alfalfa herbage.Key words: Alfalfa, forage quality, crude protein, in vitro digestibility, Medicago sativa L.

In vitro gas fermentation kinetics of compounded ration containing graded levels of biodegraded corncob

2020 ◽  
Vol 47 (2) ◽  
pp. 224-234
Author(s):  
O.Y. Adedeji ◽  
A. A. Saka
Keyword(s):  
Aspergillus Niger ◽  
Crude Protein ◽  
Nutritive Value ◽  
Gas Production ◽  
Corn Cob ◽  
Crude Fibre ◽  
In Vitro Gas Production ◽  
Dietary Treatments ◽  
Gas Volume

Cob regarded as a waste product, constituting source of environmental pollution could be utilized as an energy source in feed formulation if properly processed and harnessed through fungal degradation. Hence, this study was designed to evaluate the nutritive value of biodegraded corncob meal based diet using in vitro gas production technique. Aspergillus niger was isolated and sub-cultured to obtain a pure culture. Degraded corn cob meals(DCCM) of four dietary treatments were prepared to include: T (0% DCCM which served as 1 the control), T (15% DCCM), T (30% DCCM) and T (45% DCCM). Each diet sample 2 3 4 (200mg) was incubated in buffered rumen liquor for 48 hours and gas volume was estimated using established in vitro gas production models. Amount of gas volume produced was determined every 3 hours for 48 hours of incubation in buffered rumen fluid. After 48 hours ofincubation, methane gas produced was estimated and determined in triplicates. The proximate composition of Aspergillus niger biodegraded corn cob indicated that Aspergillus niger improved the nutritive value of the corn cob. Untreated corn cob meal was lower in nutritive values when compared with treated corncob meal with crude protein, crude fibre and ash values ranging from 6.88 to 9.78%, 32.68 to 26.37% and 2.87 to 2.88% respectively.The proximate composition of the dietary treatments showed that the crude protein varied from (11.67-12.67%), crude fibre (10.94-21.56%), ether extract (2.12-4.88%), ash (6.48- 9.44%) and nitrogen free extract (58.17-62.99%). Results obtained for volume of gas (35.960-72.770mL/200mgDM) produced in time “t” denoted by (b) were significantly different (P<0.05) across the dietary treatments. However, rate of gas production (0.0297- 0.0425mL/hr) and time between incubation and gas production (2.083-2.683hr) were not significantly different (P>0.05) across the dietary treatments. Cumulative gas volumeproduction at 24 hours were significantly (P<0.05) influenced by different inclusion levels of DCCM with values obtained ranging from T (15.33 ml/gDM) to T (35.33 ml/gDM). There 4 3 were significant differences (P<0.05) across the dietary treatments. The post estimated parameters ranging from metabolisable energy (4.96-7.74MJ/Kg DM), organic matter digestibility (37.98-58.03%), short chain fatty acids (0.31-0.78μmol), Methane estimate (3-8  ml/200mgDM) and Carbon dioxide estimate (10-30 ml/200mgDM). It can be concluded that 30% DCCM based diet had the potential of meeting the nutritional needs as smallruminant livestock feeds, if properly biodegraded and incorporated into feeds.

Effect of growth stage at cutting on yield and quality of lucerne cultivars from different dormancy groups in northern Victoria

1987 ◽  
Vol 27 (1) ◽  
pp. 55 ◽  
Author(s):  
RH Slarke ◽  
WK Mason
Keyword(s):  
Protein Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Growth Stage ◽  
In Vitro Digestibility ◽  
Growth Stages ◽  
Flower Bud ◽  
Yield And Quality

At Kyabram, Victoria, the effects of growth stage at cutting on dry matter (DM) yield and quality of lucerne was determined during the warm season haymaking period for cultivars with contrasting winter dormancy characteristics. Growth stages at cutting were pre-flower bud, flower-bud, 10% bloom and full bloom. Cultivars were winter non-dormant CUF 101 and Pioneer Brand 572, semi-winter dormant Pioneer Brand 581 and winter dormant Pioneer Brand 545. Cultivar responses or interactions between cultivar and growth stage at cutting were not significant (P>0.05) for dry matter yield, crude protein or in vitro digestibility. Cutting of the pre-flower bud stage, compared with cutting at the 10% bloom stage, reduced DM yield by 18% (16.4 v. 13.5 t/ha), but increased crude protein content of the lucerne from 19.3 to 24%. The total protein yield per hectare was not affected by cutting stages from pre-flower bud stage to 10% bloom. Cutting lucerne at the flower bud stage rather than at 10% bloom gave increased protein content and digestibility with only a moderate yield decline. However, more frequent cutting was unsatisfactory as it decreased the density of the stand and increased the proportion of weeds and so could not be recommended.

scholarly journals Nutritional potentials and in vitro estimation of composite cocoa pod husk-based diets for ruminants

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Oluwatosin Bode Omotoso
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
In Vitro Study ◽  
Metabolizable Energy ◽  
Urea Solution ◽  
In Vitro Digestibility ◽  
Cocoa Pod Husk ◽  
Cassava Peel

Abstract Background The use of cocoa pod husk in livestock nutrition is being limited because of its fibrous nature and anti-nutrients, which have detrimental effect(s) on the animals. This study was conducted to evaluate the nutritive value and effects of replacing urea-treated ensiled cocoa pod husk meal (urea-treated CPHM) with cassava peel in a complete concentrate diet (CCD) on in vitro digestibility and methane (CH4) production. Methods Urea-treated CPHM was prepared by soaking the raw milled pod in 5% urea solution under anaerobic condition for 7 days, and decanted and the filtrates were further ensiled for 28 days, dried, and were replaced with cassava peel meal in CCD at 0, 5, 7.5, 10, 12.5, and 15% on part basis. Feed samples (n = 3) were analyzed for chemical composition, and data generated from in vitro study were subjected to statistical analysis in a completely randomized design experiment. Results Results revealed that the treatment significantly reduced the crude fiber, fiber fraction contents, and anti-nutrients and improved the crude protein content of the pod by 71.84%. Dry matter and crude protein of the diets ranged from 89.34–89.71% and 10.52–12.84%, respectively. The in vitro dry matter digestibility (IVDMD) increased with increasing levels of urea-treated CPHM in the diets. With increasing levels of urea-treated CPHM, CH4 production relatively reduced as compared to diet E. Metabolizable energy (5.66 Kcal/g) of diet F was the highest. Therefore, the inclusion of urea-treated CPHM at 15% in CCDs has the potential for improving digestibility and reducing CH4 production in ruminants.

scholarly journals Evaluasi Nilai Nutrisi dan Kecernaan In Vitro Pelepah Kelapa Sawit (Oil Palm Fronds) yang Difermentasi Menggunakan Aspergillus niger dengan Penambahan Sumber Karbohidrat yang Berbeda

2015 ◽  
Vol 15 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Sitti Wajizah ◽  
Samadi Samadi ◽  
Yunasri Usman ◽  
Elmy Mariana
Keyword(s):  
Aspergillus Niger ◽  
Oil Palm ◽  
Crude Protein ◽  
Nutritive Value ◽  
Crude Fiber ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Oil Palm Fronds ◽  
Palm Fronds

(The evaluation of  nutritive value and In Vitro digestibility of oil palm fronds through fermentation by using  Aspergillus niger  with  different soluble carbohydrate sources) ABSTRACT. Oil palm frond (OPF) is one of potential sources of alternative feed, but has limited use due to high crude fiber and low crude protein contents. Fermentation is one of the methods widely applied to improve nutritive value of animal feed. The purpose of this research is to increase nutritive value of fermented oil palm fronds by adding different soluble carbohydrate source into fermentation media. The results of the experiments indicated that fermented oil palm fronds by Aspergillus niger had a significant effect (P0,05)  on the content of crude protein, crude fiber, and ash. Generally, fermented oil palm fronds with different soluble carbohydrate was able to increase the content of crude protein of oil palm fronds, but not optimal yet in reducing the crude fiber content of fermented substrate. However, the addition of rice bran on the fermentation medium showed the best results, characterized by increasing crude protein and decreasing crude fiber contents as well as improved dry matter and organic matter digestibility, reflected in high concentration of VFA. 

scholarly journals Screening white clover cultivars- for improved nutritive value - development of a method

1994 ◽  
pp. 49-53
Author(s):  
G.C. Waghorn ◽  
J.R. Caradus
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Nutritive Value ◽  
Nitrogen Loss ◽  
Protein Solubility ◽  
High Solubility ◽  
Mean Values ◽  
Rumen Degradation ◽  
Low Solubility

A method has been developed which enabled 100 white clover cultivars to be screened and ranked for improved nutritive value. Our objective was to identify cultivars having protein which was relatively insoluble and resistant to rumen degradation. The methods were developed for fresh forages, so that the results were applicable to the grazing animal. The clover was harvested and minced to give a particle size distribution similar to material chewed by ruminants during eating. Crude protein solubility was estimated by measuring nitrogen (N) loss from minced clover incubated in nylon bags in the rumen of a cow. This required the optimal amount of dry matter (DM) and incubation times to be determined, variation between animals to be defined and repeatability of measurements established. Crude protein degradation was measured by incubating minced clover with buffered rumen bacteria in vitro and determining net conversion of plant N to ammonia N. Nitrogen loss to degradation and cultivars w&e identified as having low solubility _ ability-for-the-1.OO-cultivar-were-positively and slow degradation and five cultivars having high solubility and rapid degradation. Mean values correlated (r=+O.29; P

Preservation of wet rice straw using urea and molasses in monsoon of Bangladesh

2020 ◽  
Vol 01 (02) ◽  
pp. 36-44
Author(s):  
R. Sultana ◽  
D. K. Jamee ◽  
S. Islam ◽  
S. M. A. Islam ◽  
M. R. Amin ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Rice Straw ◽  
Nutritive Value ◽  
Fungal Growth ◽  
Gas Production ◽  
Metabolizable Energy ◽  
In Vitro Digestibility ◽  
Crude Fibre ◽  
Physical Quality

An experiment of treated and ensiled wet rice straw with urea and molasses was performed to explore the chemical composition, physical quality, in vitro digestibility and its potentiality as a quality feed for ruminants. In this experiment, plastic containers were used to preserve chopped wet rice straw under airtight condition based on the treatment as T1 (wet rice straw only), T2 (straw with 5% molasses), T3 (straw with 5% urea), T4 (straw with 5% urea and 5% molasses) and T5 (straw with 10% urea and 5% molasses) to analyze chemical composition, physical quality, metabolizable energy (ME) content, in vitro organic matter digestibility (OMD) and in vitro gas production (IVGP) at five different ensiling times of 0, 30, 45, 60 and 90 days. The physical quality (color, smell, softness characteristics) of wet rice straw was improved with urea and molasses treatment. Treatments T5 was found better as there was no fungal growth till 90 days of ensiling. The addition of urea and molasses improved the physical quality, nutritive value and preservation quality of wet rice straw. Urea and molasses treated and ensiled (T5) straw showed better color, nutritional quality, softness and longer preservation capacity compared with all treatments followed by T4, T2 and T3. The crude protein (CP) content was increased (P<0.05) but the crude fibre (CF), dry matter (DM), ash contents and ether extract (EE) were decreased (P<0.05) in all of the treatments (T2, T3, T4 and T5) compared to control (T1). The OMD, IVGP and ME contents were increased in all of the treatments (T2, T3, T4 and T5) compared to control (T1). The highest OMD, IVGP and ME values were observed in treatment T5 and the lowest values were in control (T1) Through the Consideration of all the chemical and physical properties, among all of the treatments, 10% urea and 5% molasses are found acceptable for the preservation of rice straw. Thus, environment friendly and cost effective feed can be formulated.

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