scholarly journals Growth temperatures and electrophoretic karyotyping as tools for practical discrimination of saccharomyces bayanus and saccharomyces cerevisiae.

1995 ◽  
Vol 41 (3) ◽  
pp. 239-247 ◽  
Author(s):  
MUNEKAZU KISHIMOTO ◽  
SHOJI GOTO
1996 ◽  
Vol 42 (4) ◽  
pp. 335-339 ◽  
Author(s):  
Gennadi I. Naumov ◽  
Elena S. Naumova ◽  
Enrique D. Sancho

Using genetic hybridization analysis, electrophoretic karyotyping, and Southern hybridization with the ADC1 promoter probe, three biological sibling species, Saccharomyces cerevisiae, Saccharomyces paradoxus, and Saccharomyces bayanus, have been identified in Ontario and California. Saccharomyces kluyveri strains were revealed by karyotyping.Key words: genetical taxonomy, sibling species, Saccharomyces complex, electrophoretic karyotyping.


2002 ◽  
Vol 153 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Federico Sebastiani ◽  
Claudia Barberio ◽  
Enrico Casalone ◽  
Duccio Cavalieri ◽  
Mario Polsinelli

1998 ◽  
Vol 44 (11) ◽  
pp. 1045-1050 ◽  
Author(s):  
Gennadi I Naumov ◽  
Elena S Naumova ◽  
Paul D Sniegowski

Genetic hybridization and karyotypic analyses revealed the biological species Saccharomyces paradoxus and Saccharomyces cerevisiae in exudates from North American oaks for the first time. In addition, two strains collected from elm flux and from Drosophila by Phaff in 1961 and 1952 were reidentified as S. paradoxus. Each strain studied showed a unique profile of chromosomal hybridization with a probe for the retrotransposable element Ty1. The wild distribution of natural Saccharomyces sensu stricto yeasts is discussed.Key words: genetical taxonomy, Saccharomyces paradoxus, oak exudates, Ty elements, electrophoretic karyotyping.


2018 ◽  
Author(s):  
Ömur Kayikci ◽  
Paul M. Magwene

ABSTRACTThe cyclic AMP – Protein Kinase A (cAMP–PKA) pathway is an evolutionarily conserved eukaryotic signaling network that is essential for growth and development. In the fungi, cAMP–PKA signaling plays a critical role in regulating cellular physiology and morphological switches in response to nutrient availability. We undertook a comparative investigation of the role that cAMP-PKA signaling plays in the regulation of filamentous growth in two closely related budding yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus. Using chemical and genetic perturbations of this pathway and its downstream targets we discovered divergent roles for cAMP-PKA signaling in the regulation of filamentous growth. While cAMP-PKA signaling is required for the filamentous growth response in both species, increasing or decreasing the activity of this pathway leads to drastically different phenotypic outcomes. In S. cerevisiae, cAMP-PKA inhibition ameliorates the filamentous growth response while hyper-activation of the pathway leads to increased filamentous growth; the same perturbations in S. bayanus result in the obverse. Divergence in the regulation of filamentous growth between S. cerevisiae and S. bayanus extends to downstream targets of PKA, including several kinases, transcription factors, and effector proteins. Our findings highlight the potential for significant evolutionary divergence in gene network function, even when the constituent parts of such networks are well conserved.


OENO One ◽  
1980 ◽  
Vol 14 (3) ◽  
pp. 155
Author(s):  
Simone Sapis-domercq

<p style="text-align: justify;">A la suite de précédents travaux d'identification de la microflore des raisins traités par divers fongicides, on étudie dans ce travail l'influence de ces produits (notamment anti-mildiou et anti-<em>Botrytis</em>), sur les espèces de levures et de bactéries les plus fréquemment rencontrées au cours de la vinification. <em>Hanseniaspora uvarum</em>, <em>Saccharomyces cerevisiae</em>, <em>Saccharomyces bayanus</em> sont sensibles à l'action de l'Euparène, du Cuprosan et du Mycodifol. Par contre, les bactéries lactiques et surtout acétiques sont peu sensibles. Par ailleurs, les levures les plus résistantes ne sont pas suffisamment alcoogènes pour assurer l'achèvement de la fermentation alcoolique jusqu'à épuisement du sucre.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Following preceding work about microflora identification of pesticides treated grapes, the influence of some of these products (especially anti-mildiou and anti-<em>Botrytis</em>) on usual types of yeasts and bacteria involved in wine technology was studied. <em>Hanseniaspora uvarum</em>, <em>Saccharomyces cerevisiae</em>, <em>Saccharomyces bayanus</em> are sensitive to Euparène, Cuprosan and Mycodifol. Lactic and acetic bacteria are less sensitive. Howewer, the highest resistant yeasts have not an alcoholic power sufficient to achieve the fermentation of the sugar up to complete consumption.</p>


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