Genetic reidentification of Saccharomyces strains associated with black knot disease of trees in Ontario and Drosophila species in California

1996 ◽  
Vol 42 (4) ◽  
pp. 335-339 ◽  
Author(s):  
Gennadi I. Naumov ◽  
Elena S. Naumova ◽  
Enrique D. Sancho
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sibling Species ◽  
Southern Hybridization ◽  
Hybridization Analysis ◽  
Drosophila Species ◽  
Saccharomyces Paradoxus ◽  
Electrophoretic Karyotyping ◽  
Saccharomyces Kluyveri ◽  
Saccharomyces Bayanus ◽  
Promoter Probe

Using genetic hybridization analysis, electrophoretic karyotyping, and Southern hybridization with the ADC1 promoter probe, three biological sibling species, Saccharomyces cerevisiae, Saccharomyces paradoxus, and Saccharomyces bayanus, have been identified in Ontario and California. Saccharomyces kluyveri strains were revealed by karyotyping.Key words: genetical taxonomy, sibling species, Saccharomyces complex, electrophoretic karyotyping.

Saccharomyces paradoxus and Saccharomyces cerevisiae are associated with exudates of North American oaks

1998 ◽  
Vol 44 (11) ◽  
pp. 1045-1050 ◽  
Author(s):  
Gennadi I Naumov ◽  
Elena S Naumova ◽  
Paul D Sniegowski
Keyword(s):  
Saccharomyces Cerevisiae ◽  
North American ◽  
Sensu Stricto ◽  
Biological Species ◽  
Saccharomyces Paradoxus ◽  
Saccharomyces Sensu Stricto ◽  
Ty Elements ◽  
Electrophoretic Karyotyping ◽  
Retrotransposable Element ◽  
First Time

Genetic hybridization and karyotypic analyses revealed the biological species Saccharomyces paradoxus and Saccharomyces cerevisiae in exudates from North American oaks for the first time. In addition, two strains collected from elm flux and from Drosophila by Phaff in 1961 and 1952 were reidentified as S. paradoxus. Each strain studied showed a unique profile of chromosomal hybridization with a probe for the retrotransposable element Ty1. The wild distribution of natural Saccharomyces sensu stricto yeasts is discussed.Key words: genetical taxonomy, Saccharomyces paradoxus, oak exudates, Ty elements, electrophoretic karyotyping.

scholarly journals Mitochondrial DNA duplication, recombination, and introgression during interspecific hybridization

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Silvia Bágeľová Poláková ◽  
Žaneta Lichtner ◽  
Tomáš Szemes ◽  
Martina Smolejová ◽  
Pavol Sulo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Interspecific Hybridization ◽  
Mobile Elements ◽  
Variable Length ◽  
Mitochondrial Genomes ◽  
Free Standing ◽  
Saccharomyces Paradoxus ◽  
Mtdna Recombination

AbstractmtDNA recombination events in yeasts are known, but altered mitochondrial genomes were not completed. Therefore, we analyzed recombined mtDNAs in six Saccharomyces cerevisiae × Saccharomyces paradoxus hybrids in detail. Assembled molecules contain mostly segments with variable length introgressed to other mtDNA. All recombination sites are in the vicinity of the mobile elements, introns in cox1, cob genes and free standing ORF1, ORF4. The transplaced regions involve co-converted proximal exon regions. Thus, these selfish elements are beneficial to the host if the mother molecule is challenged with another molecule for transmission to the progeny. They trigger mtDNA recombination ensuring the transfer of adjacent regions, into the progeny of recombinant molecules. The recombination of the large segments may result in mitotically stable duplication of several genes.

Isolation, screening, identification and tolerance of yeast in cherry wine lees

2020 ◽  
Vol 16 (9) ◽  
Author(s):  
Cheng Xu ◽  
Hui Xia ◽  
Shuwen Zhang ◽  
Yuping Zhao ◽  
Zhiqiang Qi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Potassium Chloride ◽  
Rose Bengal ◽  
Morphological Observation ◽  
Good Tolerance ◽  
Saccharomyces Paradoxus ◽  
Subsequent Application ◽  
Biological Identification ◽  
Tolerance Study ◽  
Saccharomyces Kudriavzevii

AbstractIn this study, yeast was isolated from cherry wine lees by rose Bengal medium, and its species was identified through three-stage screening, morphological observation and molecular biological identification. Moreover, the tolerance of screened strains was studied. The results showed that 30 strains of yeast were isolated from cherry wine lees, and five strains of yeast were selected, which were named YJN10, YJN16, YJN18, YJN19 and YJN28. After preliminary appraisal, strain YJN10 was Saccharomyces kudriavzevii, strain YJN16 was Saccharomyces paradoxus, and strains YJN18, YJN19, YJN28 were Saccharomyces cerevisiae. In the tolerance study, the tolerable sugar concentrations of the five strains were 650, 650, 550, 600 and 600 g/L. The tolerable alcohol volume fractions were 20, 20, 16, 18 and 18%. The tolerable molar concentration of potassium chloride was 1.8, 1.8, 1.5, 1.5 and 1.5 mol/L. Finally, strains YJN10, YJN16, YJN19 and YJN28 showed good tolerance, which laid a foundation for subsequent application in cherry wine fermentation.

Substitutions in the hydrophobic core of the alpha-factor receptor of Saccharomyces cerevisiae permit response to Saccharomyces kluyveri alpha-factor and to antagonist

1992 ◽  
Vol 12 (9) ◽  
pp. 3959-3966
Author(s):  
L Marsh
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Receptor Gene ◽  
Hydrophobic Core ◽  
Ligand Specificity ◽  
Cycle Arrest ◽  
Saccharomyces Kluyveri ◽  
Alpha Factor ◽  
Mutant Receptors ◽  
In Vivo Screen

Mutations in the Saccharomyces cerevisiae alpha-factor receptor that lead to improved response to Saccharomyces kluyveri alpha-factor were identified and sequenced. Mutants were isolated from cells bearing randomly mutagenized receptor gene (STE2) plasmids by an in vivo screen. Five mutations lead to substitutions in hydrophobic segments in the core of the receptor (M54I, S145L, S145L-S219L, A229V, L255S-S288P). Remarkably, strains expressing these mutant receptors exhibited positive pheromone responses to desTrp1,Ala3-alpha-factor, an analog that normally blocks these responses. The M54I mutation appeared to affect only ligand specificity. The other mutations conferred additional effects on signaling or recovery. Two mutants were more sensitive to alpha-factor than wild type (S145L, A229V). One mutant was more sensitive to alpha-factor-induced cell cycle arrest initially, but then recovered more efficiently (S145L-S219L). One mutant (L255S-S288P) conferred positive pheromone responses to alpha-factor as assayed by FUS1-lacZ reporter induction, but did not display growth arrest. The hydrophobic receptor core thus appears to control activation by some ligands and to play roles in aspects of signal transduction and recovery.

S elements: a family of Tc1-like transposons in the genome of Drosophila melanogaster.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1425-1438 ◽  
Author(s):  
P J Merriman ◽  
C D Grimes ◽  
J Ambroziak ◽  
D A Hackett ◽  
P Skinner ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Amino Acid ◽  
Transposable Elements ◽  
Sibling Species ◽  
Inverted Repeat ◽  
Open Reading Frame ◽  
Drosophila Species ◽  
Insertion Mutation ◽  
Reading Frame ◽  
Diploid Genome

Abstract The S elements form a diverse family of long-inverted-repeat transposons within the genome of Drosophila melanogaster. These elements vary in size and sequence, the longest consisting of 1736 bp with 234-bp inverted terminal repeats. The longest open reading frame in an intact S element could encode a 345-amino acid polypeptide. This polypeptide is homologous to the transposases of the mariner-Tc1 superfamily of transposable elements. S elements are ubiquitous in D. melanogaster populations and also appear to be present in the genomes of two sibling species; however, they seem to be absent from 17 other Drosophila species that were examined. Within D. melanogaster strains, there are, on average, 37.4 cytologically detectable S elements per diploid genome. These elements are scattered throughout the chromosomes, but several sites in both the euchromatin and beta heterochromatin are consistently occupied. The discovery of an S-element-insertion mutation and a reversion of this mutation indicates that S elements are at least occasionally mobile in the D. melanogaster genome. These elements seem to insert at an AT dinucleotide within a short palindrome and apparently duplicate that dinucleotide upon insertion.

scholarly journals Suppressive Effect of Wild Saccharomyces cerevisiae and Saccharomyces paradoxus Strains on Ige Production by Mouse Spleen Cells

2013 ◽  
Vol 19 (6) ◽  
pp. 1019-1027 ◽  
Author(s):  
Takeshi KAWAHARA ◽  
Daichi NAKAYAMA ◽  
Keisuke TODA ◽  
Shyuichiro INAGAKI ◽  
Katsumi TANAKA ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Suppressive Effect ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Saccharomyces Paradoxus
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