Functional properties of Saccharomyces kluyveri Y97-fermented solo black garlic

Setiyoningrum F, Pribadi G, Afiati F, Herlina N, Solikhin A, Lisani N. 2018. Functional properties of Saccharomyces kluyveri Y97-fermented solo black garlic. Asian J Agric 2: 48-51. Saccharomyces kluyveri Y97-fermented solo black garlic was made by fermentation of fresh solo garlic in medium containing S. kluyveri aging in 70C and relative humidity close to 60%. The fermentation period of fresh solo garlic in the medium was 0, 2, 4 dan 6 days. The black aging period was 0 (fresh garlic), 7, 14 dan 21 days. Antioxidant capacity, flavonoid content and total poliphenol were observed. S. kluyveri Y97-fermented solo black garlic had higher antioxidant capacity, flavonoid content and total polifenol compared to solo black garlic without fermentation. Fermentation of fresh solo garlic by S. kluyveri Y97 before aging process could increase solo black garlic’s functional properties.

Saccharomyces Kluyveri Fungemia in a Patient with Human Immunodeficiency Virus Infection

We report a case of a young woman with acquired immune deficiency syndrome admitted with fever and abdominal pain. Saccharomyces kluyveri was isolated in blood culture. She was successfully treated with fluconazole. This case demonstrates the pathogenicity of Saccharomyces Kluyveri in immunocompromised host.

Global Expression Analysis of the Yeast Lachancea (Saccharomyces) kluyveri Reveals NewURCGenes Involved in Pyrimidine Catabolism

ABSTRACTPyrimidines are important nucleic acid precursors which are constantly synthesized, degraded, and rebuilt in the cell. Four degradation pathways, two of which are found in eukaryotes, have been described. One of them, theURCpathway, has been initially discovered in our laboratory in the yeastLachancea kluyveri. Here, we present the global changes in gene expression inL. kluyveriin response to different nitrogen sources, including uracil, uridine, dihydrouracil, and ammonia. The expression pattern of the knownURCgenes,URC1-6, helped to identify nine putative novelURCgenes with a similar expression pattern. The microarray analysis provided evidence that both theURCandPYDgenes are under nitrogen catabolite repression inL. kluyveriand are induced by uracil or dihydrouracil, respectively. We determined the function ofURC8, which was found to catalyze the reduction of malonate semialdehyde to 3-hydroxypropionate, the final degradation product of the pathway. The other eight genes studied were all putative permeases. Our analysis of double deletion strains showed that theL. kluyveriFui1p protein transported uridine, just like its homolog inSaccharomyces cerevisiae, but we demonstrated that is was not the only uridine transporter inL. kluyveri. We also showed that theL. kluyverihomologs ofDUR3andFUR4do not have the same function that they have inS. cerevisiae, where they transport urea and uracil, respectively. InL. kluyveri, both of these deletion strains grew normally on uracil and urea.

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.