Reciprocal translocation at duplicated RPL2 loci might cause speciation of Saccharomyces bayanus and Saccharomyces cerevisiae

1998 ◽  
Vol 33 (5) ◽  
pp. 345-351 ◽  
Author(s):  
Seung-Lim Ryu ◽  
Yoshikatsu Murooka ◽  
Y. Kaneko
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Reciprocal Translocation ◽  
Saccharomyces Bayanus

scholarly journals Deletions extending from a single Ty1 element in Saccharomyces cerevisiae.

1985 ◽  
Vol 5 (12) ◽  
pp. 3451-3457 ◽  
Author(s):  
K M Downs ◽  
G Brennan ◽  
S W Liebman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome C ◽  
Reciprocal Translocation ◽  
Restriction Site ◽  
Chromosomal Rearrangements ◽  
Yeast Cells ◽  
Site Specific ◽  
Site Specific Recombination ◽  
The Third ◽  
Deletion Junction

Chromosomal rearrangements associated with one Ty1 element in the iso-1-cytochrome c (CYC1) region of Saccharomyces cerevisiae yeast cells were examined. Most of the rearrangements were deletions of the three linked genes, CYC1, OSM1, and RAD7, and resulted from recombination involving the single Ty1 element and a solo delta in the same orientation. These deletions differed by the number of Ty1 elements (zero, one, or two) remaining after deletion and by restriction site heterogeneities associated with these elements. A single Ty1 element remained at the deletion junction point much more frequently than no Ty1. Apparently the Ty1-associated delta element nearer to the solo delta was involved more often in recombination than the more distal Ty1-associated delta element. The restriction site data implicate gene conversion and suggest that site-specific recombination within the deltas, if occurring, is not the only mechanism of delta-delta recombination. Three other rearrangements bore deletions which began at the end of the Ty1 element and extended into regions not bearing Ty1 or delta sequences. Two of these deletions eliminated 7 kilobases of DNA, although they differed by an associated reciprocal translocation. The third involved a deletion of 14.7 kilobases of DNA associated with an overlapping inversion.

Crosses between Saccharomyces cerevisiae and Saccharomyces bayanus generate fertile hybrids

2002 ◽  
Vol 153 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Federico Sebastiani ◽  
Claudia Barberio ◽  
Enrico Casalone ◽  
Duccio Cavalieri ◽  
Mario Polsinelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Saccharomyces Bayanus ◽  
Fertile Hybrids

scholarly journals Divergent Roles for cAMP–PKA Signaling in the Regulation of Filamentous Growth in Saccharomyces cerevisiae and Saccharomyces bayanus

2018 ◽  
Author(s):  
Ömur Kayikci ◽  
Paul M. Magwene
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Response ◽  
Critical Role ◽  
Filamentous Growth ◽  
Comparative Investigation ◽  
Effector Proteins ◽  
Evolutionary Divergence ◽  
Downstream Targets ◽  
Network Function ◽  
Saccharomyces Bayanus

ABSTRACTThe cyclic AMP – Protein Kinase A (cAMP–PKA) pathway is an evolutionarily conserved eukaryotic signaling network that is essential for growth and development. In the fungi, cAMP–PKA signaling plays a critical role in regulating cellular physiology and morphological switches in response to nutrient availability. We undertook a comparative investigation of the role that cAMP-PKA signaling plays in the regulation of filamentous growth in two closely related budding yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus. Using chemical and genetic perturbations of this pathway and its downstream targets we discovered divergent roles for cAMP-PKA signaling in the regulation of filamentous growth. While cAMP-PKA signaling is required for the filamentous growth response in both species, increasing or decreasing the activity of this pathway leads to drastically different phenotypic outcomes. In S. cerevisiae, cAMP-PKA inhibition ameliorates the filamentous growth response while hyper-activation of the pathway leads to increased filamentous growth; the same perturbations in S. bayanus result in the obverse. Divergence in the regulation of filamentous growth between S. cerevisiae and S. bayanus extends to downstream targets of PKA, including several kinases, transcription factors, and effector proteins. Our findings highlight the potential for significant evolutionary divergence in gene network function, even when the constituent parts of such networks are well conserved.

scholarly journals Etude de l'influence des produits de traitement de la vigne sur la microflore des raisins et des vins. Expérimentation 1978-1979. Comparaison avec les résultats de 1975, 1976 et 1977

OENO One ◽  
1980 ◽  
Vol 14 (3) ◽  
pp. 155
Author(s):  
Simone Sapis-domercq
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hanseniaspora Uvarum ◽  
Saccharomyces Bayanus ◽  
Preceding Work

<p style="text-align: justify;">A la suite de précédents travaux d'identification de la microflore des raisins traités par divers fongicides, on étudie dans ce travail l'influence de ces produits (notamment anti-mildiou et anti-<em>Botrytis</em>), sur les espèces de levures et de bactéries les plus fréquemment rencontrées au cours de la vinification. <em>Hanseniaspora uvarum</em>, <em>Saccharomyces cerevisiae</em>, <em>Saccharomyces bayanus</em> sont sensibles à l'action de l'Euparène, du Cuprosan et du Mycodifol. Par contre, les bactéries lactiques et surtout acétiques sont peu sensibles. Par ailleurs, les levures les plus résistantes ne sont pas suffisamment alcoogènes pour assurer l'achèvement de la fermentation alcoolique jusqu'à épuisement du sucre.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Following preceding work about microflora identification of pesticides treated grapes, the influence of some of these products (especially anti-mildiou and anti-<em>Botrytis</em>) on usual types of yeasts and bacteria involved in wine technology was studied. <em>Hanseniaspora uvarum</em>, <em>Saccharomyces cerevisiae</em>, <em>Saccharomyces bayanus</em> are sensitive to Euparène, Cuprosan and Mycodifol. Lactic and acetic bacteria are less sensitive. Howewer, the highest resistant yeasts have not an alcoholic power sufficient to achieve the fermentation of the sugar up to complete consumption.</p>

FTIR spectroscopic discrimination of Saccharomyces cerevisiae and Saccharomyces bayanus strains

2010 ◽  
Vol 56 (9) ◽  
pp. 793-801 ◽  
Author(s):  
Isabelle Adt ◽  
Achim Kohler ◽  
Sabine Gognies ◽  
Julien Budin ◽  
Christophe Sandt ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gel Electrophoresis ◽  
Electrophoretic Pattern ◽  
Pulsed Field Gel Electrophoresis ◽  
Infrared Absorption Spectroscopy ◽  
Single Measurement ◽  
Saccharomyces Bayanus ◽  
Components Analysis ◽  
Yeast Classification ◽  
The One

In this study, we tested the potential of Fourier-transform infrared absorption spectroscopy to screen, on the one hand, Saccharomyces cerevisiae and non-S. cerevisiae strains and, on the other hand, to discriminate between S. cerevisiae and Saccharomyces bayanus strains. Principal components analysis (PCA), used to compare 20 S. cerevisiae and 21 non-Saccharomyces strains, showed only 2 misclassifications. The PCA model was then used to classify spectra from 14 Samos strains. All 14 Samos strains clustered together with the S. cerevisiae group. This result was confirmed by a routinely used electrophoretic pattern obtained by pulsed-field gel electrophoresis. The method was then tested to compare S. cerevisiae and S. bayanus strains. Our results indicate that identification at the strain level is possible. This first result shows that yeast classification and S. bayanus identification can be feasible in a single measurement.

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