Correlation between Endoglucanase Secretion and Cell Wall Strength in Oomycete Hyphae: Implications for Growth and Morphogenesis

Mycologia ◽  
1997 ◽  
Vol 89 (5) ◽  
pp. 777 ◽  
Author(s):  
Nicholas P. Money ◽  
Terry W. Hill
Keyword(s):  
Cell Wall ◽  
Wall Strength

scholarly journals A Kinesin-Like Protein Is Essential for Oriented Deposition of Cellulose Microfibrils and Cell Wall Strength

2002 ◽  
Vol 14 (12) ◽  
pp. 3101-3117 ◽  
Author(s):  
Ruiqin Zhong ◽  
David H. Burk ◽  
W. Herbert Morrison ◽  
Zheng-Hua Ye
Keyword(s):  
Cell Wall ◽  
Cellulose Microfibrils ◽  
Wall Strength

Mutations in SNF1 complex genes affect yeast cell wall strength

2013 ◽  
Vol 92 (12) ◽  
pp. 383-395 ◽  
Author(s):  
Katja Backhaus ◽  
Dorthe Rippert ◽  
Clemens J. Heilmann ◽  
Alice G. Sorgo ◽  
Chris G. de Koster ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Yeast Cell Wall ◽  
Wall Strength

Cell wall strength of Hansenula polymorpha in continuous cultures in relation to the recovery of methanol oxidase (MOX)

1987 ◽  
Vol 27 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Marco L. F. Giuseppin ◽  
Hendrikus M. J. van Eijk ◽  
Marja Hellendoorn ◽  
José W. van Almkerk
Keyword(s):  
Cell Wall ◽  
Hansenula Polymorpha ◽  
Continuous Cultures ◽  
Wall Strength ◽  
Methanol Oxidase

scholarly journals Plant Biomass Recalcitrance: Effect of Hemicellulose Composition on Nanoscale Forces that Control Cell Wall Strength

2013 ◽  
Vol 135 (51) ◽  
pp. 19048-19051 ◽  
Author(s):  
Rodrigo L. Silveira ◽  
Stanislav R. Stoyanov ◽  
Sergey Gusarov ◽  
Munir S. Skaf ◽  
Andriy Kovalenko
Keyword(s):  
Cell Wall ◽  
Plant Biomass ◽  
Control Cell ◽  
Biomass Recalcitrance ◽  
Wall Strength

Visualization of the anionic sites in the cell wall of apple fruit using a cationic colloidal gold probe

1993 ◽  
Vol 51 ◽  
pp. 320-321
Author(s):  
Stéphane Roy ◽  
William S. Conway ◽  
Alley E. Watada ◽  
Christopher D. Pooley ◽  
William P. Wergin
Keyword(s):  
Cell Wall ◽  
Colloidal Gold ◽  
Apple Fruit ◽  
Gold Complex ◽  
Anionic Sites ◽  
Wall Strength ◽  
Anionic Binding Sites ◽  
Cationic Colloidal Gold ◽  
Softening Process ◽  
Gold Probe

The ripening of fleshy fruits involves a softening process that consists of biochemical changes in the cell wall and leads to cell separation. Calcium is an important constituent of the cell wall and plays roles in maintaining the firmness of fruit and in reducing postharvest decay. The modification of cell wall strength is believed to be influenced by calcium that interacts with acidic pectic polymers to form crossbridges. This study examined how the frequency and distribution of anionic binding sites in the cell walls of apple fruit were influenced by calcium infiltration.Mature “Golden Delicious” apple fruits were pressure infiltrated with either H2O or a 4% solution of CaCl2 and the pericarp was sampled and processed according to standard procedures. Cationic poly-Llysine colloidal gold complex was used in a one-step procedure to visualize anionic sites in muro. Observations were performed with light microscopy, following silver intensification, and with transmission electron microscopy.

scholarly journals Induced Plant Cell Wall Modifications: Use of Plant Cells with Altered Walls to Study Wall Structure, Growth and Potential for Genetic Modification

1995 ◽  
Author(s):  
Deborah Delmer ◽  
Nicholas Carpita ◽  
Abraham Marcus
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Hydrolytic Enzyme ◽  
Plant Cells ◽  
Wall Structure ◽  
Cross Linking ◽  
Cellulose Synthesis ◽  
Wall Strength ◽  
Saline Medium

Our previous work indicated that suspension-cultured plant cells show remarkable flexibility in altering cell wall structure in response either to growth on saline medium or in the presence of the cellulose synthesis inhibitor 2,-6-dichlorobenzonitrile (DCB). We have continued to analyze the structure of these modified cell walls to understand how the changes modify wall strength, porosity, and ability to expand. The major load-bearing network in the walls of DCB-adapted dicot cells that lack a substantial cellulose-xyloglucan network is comprised of Ca2+-bridged pectates; these cells also have an unusual and abundant soluble pectic fraction. By contrast, DCB-adapted barley, a graminaceous monocot achieves extra wall strength by enhanced cross-linking of its non-cellulosic polysaccharide network via phenolic residues. Our results have also shed new light on normal wall stucture: 1) the cellulose-xyloglucan network may be independent of other wall networks in dicot primary walls and accounts for about 70% of the total wall strength; 2) the pectic network in dicot walls is the primary determinant of wall porosity; 3) both wall strength and porosity in graminaceous monocot primary walls is greatly influenced by the degree of phenolic cross-linking between non-cellulosic polysaccharides; and 4) the fact that the monocot cells do not secrete excess glucuronoarabinoxylan and mixed-linked glucan in response to growth on DCB, suggests that these two non-cellulosic polymers do not normally interact with cellulose in a manner similar to xyloglucan. We also attempted to understand the factors which limit cell expansion during growth of cells in saline medium. Analyses of hydrolytic enzyme activities suggest that xyloglucan metabolism is not repressed during growth on NaCl. Unlike non-adapted cells, salt-adapted cells were found to lack pectin methyl esterase, but it is not clear how this difference could relate to alterations in wall expansibility. Salt-adaped cell walls contain reduced hyp and secrete two unique PRPP-related proteins suggesting that high NaCl inhibits the cross-linking of these proteins into the walls, a finding that might relate to their altered expansibility.

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